Genetically Diversity of Pseudomonas aeruginosa Isolated from Chronic Suppurative Otitis Media with Respect to Their Antibiotic Sensitivity Pattern
Pseudomonas aeruginosa is an important chronic suppurative otitis media (CSOM) pathogen that exhibits multiple resistances to antibiotics with increasing frequency, making patient treatment more difficult. The aim of the study is to ascertain the genetically diversity of this clinically isolated P....
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| Veröffentlicht in: | Indian journal of otolaryngology, and head, and neck surgery and head, and neck surgery, 2019-11, Vol.71 (Suppl 2), p.1300-1308 |
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| creator | Sahu, Mahesh Chandra Swain, Santosh Kumar Kar, Subrat Kumar |
| description | Pseudomonas aeruginosa
is an important chronic suppurative otitis media (CSOM) pathogen that exhibits multiple resistances to antibiotics with increasing frequency, making patient treatment more difficult. The aim of the study is to ascertain the genetically diversity of this clinically isolated
P. aeruginosa
with inter simple sequence repeat (ISSR) markers. All 25
P. aeruginosa
were isolated from CSOM patients by taking their ear swabs and culturing on blood agar and MacConkey agar. All strains were identified with morphological characters and biochemical testing. The antimicrobial susceptibility testing was carried out according to National Committee for Clinical Laboratory Standards. ISSR was used to study the genetic diversity of
P. aeruginosa
. Clinically CSOM isolated 25
P. aeruginosa
were 88% Ciprofloxacin resistant and similarly resistant to other antibiotics were documented. The study has been made using ISSR marker to find out the genomic relation among the strains/populations of
P. aeruginosa
. The result was shown that maximum similarity (80%) was between S-11 and S-13 and minimum (28.2%) was between S-4 and S-16 with an average similarity of 53.2%. The dendogram showed a distinct separation in between all the strains/populations of
P. aeruginosa
. The strains/populations were broken up into two main clusters in which small one bear two strains/populations (S-4 and S-9) and another cluster have another 23 strains/populations. These 23 strains were also separated to form subcluster by having different range of small clades. The genetically diversity of pathogenic
P. aeruginosa
present in CSOM at our hospital indicates the coexistence different strains due to different antibiotic sensitivity patterns. The conventional culture and sensitivity methods are time consuming whereas in PCR, it will detect within 4–6 h for effective antibiotic. Basing upon the banding pattern with ISSR primers, clinicians can prescribe the effective antibiotics accordingly for CSOM patients in the same day. |
| doi_str_mv | 10.1007/s12070-018-1358-8 |
| format | Article |
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is an important chronic suppurative otitis media (CSOM) pathogen that exhibits multiple resistances to antibiotics with increasing frequency, making patient treatment more difficult. The aim of the study is to ascertain the genetically diversity of this clinically isolated
P. aeruginosa
with inter simple sequence repeat (ISSR) markers. All 25
P. aeruginosa
were isolated from CSOM patients by taking their ear swabs and culturing on blood agar and MacConkey agar. All strains were identified with morphological characters and biochemical testing. The antimicrobial susceptibility testing was carried out according to National Committee for Clinical Laboratory Standards. ISSR was used to study the genetic diversity of
P. aeruginosa
. Clinically CSOM isolated 25
P. aeruginosa
were 88% Ciprofloxacin resistant and similarly resistant to other antibiotics were documented. The study has been made using ISSR marker to find out the genomic relation among the strains/populations of
P. aeruginosa
. The result was shown that maximum similarity (80%) was between S-11 and S-13 and minimum (28.2%) was between S-4 and S-16 with an average similarity of 53.2%. The dendogram showed a distinct separation in between all the strains/populations of
P. aeruginosa
. The strains/populations were broken up into two main clusters in which small one bear two strains/populations (S-4 and S-9) and another cluster have another 23 strains/populations. These 23 strains were also separated to form subcluster by having different range of small clades. The genetically diversity of pathogenic
P. aeruginosa
present in CSOM at our hospital indicates the coexistence different strains due to different antibiotic sensitivity patterns. The conventional culture and sensitivity methods are time consuming whereas in PCR, it will detect within 4–6 h for effective antibiotic. Basing upon the banding pattern with ISSR primers, clinicians can prescribe the effective antibiotics accordingly for CSOM patients in the same day.</description><identifier>ISSN: 2231-3796</identifier><identifier>EISSN: 0973-7707</identifier><identifier>DOI: 10.1007/s12070-018-1358-8</identifier><identifier>PMID: 31750169</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Antibiotics ; Bacterial infections ; Drug resistance ; Ear diseases ; Genetic diversity ; Gram-negative bacteria ; Head and Neck Surgery ; Medicine ; Medicine & Public Health ; Original ; Original Article ; Otorhinolaryngology ; Pathogens</subject><ispartof>Indian journal of otolaryngology, and head, and neck surgery, 2019-11, Vol.71 (Suppl 2), p.1300-1308</ispartof><rights>Association of Otolaryngologists of India 2018</rights><rights>Indian Journal of Otolaryngology and Head & Neck Surgery is a copyright of Springer, (2018). All Rights Reserved.</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-49f674c363ca6cb83e37ceafed59eeed694384149d7cb6cc706e763796de88553</citedby><cites>FETCH-LOGICAL-c475t-49f674c363ca6cb83e37ceafed59eeed694384149d7cb6cc706e763796de88553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6841806/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6841806/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids></links><search><creatorcontrib>Sahu, Mahesh Chandra</creatorcontrib><creatorcontrib>Swain, Santosh Kumar</creatorcontrib><creatorcontrib>Kar, Subrat Kumar</creatorcontrib><title>Genetically Diversity of Pseudomonas aeruginosa Isolated from Chronic Suppurative Otitis Media with Respect to Their Antibiotic Sensitivity Pattern</title><title>Indian journal of otolaryngology, and head, and neck surgery</title><addtitle>Indian J Otolaryngol Head Neck Surg</addtitle><description>Pseudomonas aeruginosa
is an important chronic suppurative otitis media (CSOM) pathogen that exhibits multiple resistances to antibiotics with increasing frequency, making patient treatment more difficult. The aim of the study is to ascertain the genetically diversity of this clinically isolated
P. aeruginosa
with inter simple sequence repeat (ISSR) markers. All 25
P. aeruginosa
were isolated from CSOM patients by taking their ear swabs and culturing on blood agar and MacConkey agar. All strains were identified with morphological characters and biochemical testing. The antimicrobial susceptibility testing was carried out according to National Committee for Clinical Laboratory Standards. ISSR was used to study the genetic diversity of
P. aeruginosa
. Clinically CSOM isolated 25
P. aeruginosa
were 88% Ciprofloxacin resistant and similarly resistant to other antibiotics were documented. The study has been made using ISSR marker to find out the genomic relation among the strains/populations of
P. aeruginosa
. The result was shown that maximum similarity (80%) was between S-11 and S-13 and minimum (28.2%) was between S-4 and S-16 with an average similarity of 53.2%. The dendogram showed a distinct separation in between all the strains/populations of
P. aeruginosa
. The strains/populations were broken up into two main clusters in which small one bear two strains/populations (S-4 and S-9) and another cluster have another 23 strains/populations. These 23 strains were also separated to form subcluster by having different range of small clades. The genetically diversity of pathogenic
P. aeruginosa
present in CSOM at our hospital indicates the coexistence different strains due to different antibiotic sensitivity patterns. The conventional culture and sensitivity methods are time consuming whereas in PCR, it will detect within 4–6 h for effective antibiotic. Basing upon the banding pattern with ISSR primers, clinicians can prescribe the effective antibiotics accordingly for CSOM patients in the same day.</description><subject>Antibiotics</subject><subject>Bacterial infections</subject><subject>Drug resistance</subject><subject>Ear diseases</subject><subject>Genetic diversity</subject><subject>Gram-negative bacteria</subject><subject>Head and Neck Surgery</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Original</subject><subject>Original Article</subject><subject>Otorhinolaryngology</subject><subject>Pathogens</subject><issn>2231-3796</issn><issn>0973-7707</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp9ksFu1DAURSMEokPhA9hZYsMmYMeJn7NBqgYolYpaQVlbHudlxlViB9sZNN_RH67DVCCQYOWFzz3PfrpF8ZLRN4xSeBtZRYGWlMmS8UaW8lGxoi3wEoDC42JVVZyVHFpxUjyL8ZZS3jCgT4sTzqChTLSr4u4cHSZr9DAcyHu7xxBtOhDfk-uIc-dH73QkGsO8tc5HTS6iH3TCjvTBj2S9C95ZQ77O0zQHnbKAXCWbbCSfsbOa_LBpR75gnNAkkjy52aEN5Mwlu7E-LUl0eaLdL1OvdUoY3PPiSa-HiC8eztPi28cPN-tP5eXV-cX67LI0NTSprNteQG244EYLs5EcORjUPXZNi4idaGsua1a3HZiNMAaoQBDLOjqUsmn4afHu6J3mzYidQZeCHtQU7KjDQXlt1Z83zu7U1u-VyFpJRRa8fhAE_33GmNRoo8Fh0A79HFVevwCQTbugr_5Cb_0cXP7eQnEKVXb-l6JVLYBVP13sSJngYwzY_3oyo2ophjoWQ-ViqKUYSuZMdczEzLotht_mf4fuAYPSvRE</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Sahu, Mahesh Chandra</creator><creator>Swain, Santosh Kumar</creator><creator>Kar, Subrat Kumar</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>04Q</scope><scope>04T</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20191101</creationdate><title>Genetically Diversity of Pseudomonas aeruginosa Isolated from Chronic Suppurative Otitis Media with Respect to Their Antibiotic Sensitivity Pattern</title><author>Sahu, Mahesh Chandra ; Swain, Santosh Kumar ; Kar, Subrat Kumar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-49f674c363ca6cb83e37ceafed59eeed694384149d7cb6cc706e763796de88553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antibiotics</topic><topic>Bacterial infections</topic><topic>Drug resistance</topic><topic>Ear diseases</topic><topic>Genetic diversity</topic><topic>Gram-negative bacteria</topic><topic>Head and Neck Surgery</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Original</topic><topic>Original Article</topic><topic>Otorhinolaryngology</topic><topic>Pathogens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sahu, Mahesh Chandra</creatorcontrib><creatorcontrib>Swain, Santosh Kumar</creatorcontrib><creatorcontrib>Kar, Subrat Kumar</creatorcontrib><collection>CrossRef</collection><collection>India Database</collection><collection>India Database: Health & Medicine</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Indian journal of otolaryngology, and head, and neck surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sahu, Mahesh Chandra</au><au>Swain, Santosh Kumar</au><au>Kar, Subrat Kumar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetically Diversity of Pseudomonas aeruginosa Isolated from Chronic Suppurative Otitis Media with Respect to Their Antibiotic Sensitivity Pattern</atitle><jtitle>Indian journal of otolaryngology, and head, and neck surgery</jtitle><stitle>Indian J Otolaryngol Head Neck Surg</stitle><date>2019-11-01</date><risdate>2019</risdate><volume>71</volume><issue>Suppl 2</issue><spage>1300</spage><epage>1308</epage><pages>1300-1308</pages><issn>2231-3796</issn><eissn>0973-7707</eissn><abstract>Pseudomonas aeruginosa
is an important chronic suppurative otitis media (CSOM) pathogen that exhibits multiple resistances to antibiotics with increasing frequency, making patient treatment more difficult. The aim of the study is to ascertain the genetically diversity of this clinically isolated
P. aeruginosa
with inter simple sequence repeat (ISSR) markers. All 25
P. aeruginosa
were isolated from CSOM patients by taking their ear swabs and culturing on blood agar and MacConkey agar. All strains were identified with morphological characters and biochemical testing. The antimicrobial susceptibility testing was carried out according to National Committee for Clinical Laboratory Standards. ISSR was used to study the genetic diversity of
P. aeruginosa
. Clinically CSOM isolated 25
P. aeruginosa
were 88% Ciprofloxacin resistant and similarly resistant to other antibiotics were documented. The study has been made using ISSR marker to find out the genomic relation among the strains/populations of
P. aeruginosa
. The result was shown that maximum similarity (80%) was between S-11 and S-13 and minimum (28.2%) was between S-4 and S-16 with an average similarity of 53.2%. The dendogram showed a distinct separation in between all the strains/populations of
P. aeruginosa
. The strains/populations were broken up into two main clusters in which small one bear two strains/populations (S-4 and S-9) and another cluster have another 23 strains/populations. These 23 strains were also separated to form subcluster by having different range of small clades. The genetically diversity of pathogenic
P. aeruginosa
present in CSOM at our hospital indicates the coexistence different strains due to different antibiotic sensitivity patterns. The conventional culture and sensitivity methods are time consuming whereas in PCR, it will detect within 4–6 h for effective antibiotic. Basing upon the banding pattern with ISSR primers, clinicians can prescribe the effective antibiotics accordingly for CSOM patients in the same day.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>31750169</pmid><doi>10.1007/s12070-018-1358-8</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibiotics Bacterial infections Drug resistance Ear diseases Genetic diversity Gram-negative bacteria Head and Neck Surgery Medicine Medicine & Public Health Original Original Article Otorhinolaryngology Pathogens |
| title | Genetically Diversity of Pseudomonas aeruginosa Isolated from Chronic Suppurative Otitis Media with Respect to Their Antibiotic Sensitivity Pattern |
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