Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci
Subclinical mastitis by Staphylococcus aureus (SAU) and by non-aureus staphylococci (NAS) is a major issue in the water buffalo. To understand its impact on milk, 6 quarter samples with >3,000,000 cells/mL (3 SAU-positive and 3 NAS-positive) and 6 culture-negative quarter samples with
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| Veröffentlicht in: | Scientific reports 2019-11, Vol.9 (1), p.15850-14, Article 15850 |
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| creator | Pisanu, Salvatore Cacciotto, Carla Pagnozzi, Daniela Puggioni, Giulia Maria Grazia Uzzau, Sergio Ciaramella, Paolo Guccione, Jacopo Penati, Martina Pollera, Claudia Moroni, Paolo Bronzo, Valerio Addis, Maria Filippa |
| description | Subclinical mastitis by
Staphylococcus aureus
(SAU) and by non-aureus staphylococci (NAS) is a major issue in the water buffalo. To understand its impact on milk, 6 quarter samples with >3,000,000 cells/mL (3 SAU-positive and 3 NAS-positive) and 6 culture-negative quarter samples with |
| doi_str_mv | 10.1038/s41598-019-52063-2 |
| format | Article |
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Staphylococcus aureus
(SAU) and by non-aureus staphylococci (NAS) is a major issue in the water buffalo. To understand its impact on milk, 6 quarter samples with >3,000,000 cells/mL (3 SAU-positive and 3 NAS-positive) and 6 culture-negative quarter samples with <50,000 cells/mL were investigated by shotgun proteomics and label-free quantitation. A total of 1530 proteins were identified, of which 152 were significantly changed. SAU was more impacting, with 162
vs
127 differential proteins and higher abundance changes (P < 0.0005). The 119 increased proteins had mostly structural (n = 43, 28.29%) or innate immune defence functions (n = 39, 25.66%) and included vimentin, cathelicidins, histones, S100 and neutrophil granule proteins, haptoglobin, and lysozyme. The 33 decreased proteins were mainly involved in lipid metabolism (n = 13, 59.10%) and included butyrophilin, xanthine dehydrogenase/oxidase, and lipid biosynthetic enzymes. The same biological processes were significantly affected also upon STRING analysis. Cathelicidins were the most increased family, as confirmed by western immunoblotting, with a stronger reactivity in SAU mastitis. S100A8 and haptoglobin were also validated by western immunoblotting. In conclusion, we generated a detailed buffalo milk protein dataset and defined the changes occurring in SAU and NAS mastitis, with potential for improving detection (ProteomeXchange identifier PXD012355).</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-52063-2</identifier><identifier>PMID: 31676851</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/326/2521 ; 631/45/475 ; 82 ; 82/29 ; 82/58 ; 82/80 ; Animals ; Bubalus bubalis ; Buffalo milk ; Buffaloes - metabolism ; Buffaloes - microbiology ; Butyrophilin ; Cathelicidins ; Cattle ; Cell culture ; Haptoglobin ; Histones ; Humanities and Social Sciences ; Immune system ; Immunoblotting ; Lipid metabolism ; Lysozyme ; Mastitis ; Mastitis, Bovine - metabolism ; Mastitis, Bovine - microbiology ; Milk ; Milk - metabolism ; Milk - microbiology ; Milk Proteins - biosynthesis ; multidisciplinary ; Proteins ; Proteomics ; Quantitation ; Science ; Science (multidisciplinary) ; Staphylococcal Infections - metabolism ; Staphylococcal Infections - microbiology ; Staphylococcal Infections - veterinary ; Staphylococcus aureus ; Staphylococcus aureus - metabolism ; Vimentin ; Xanthine dehydrogenase</subject><ispartof>Scientific reports, 2019-11, Vol.9 (1), p.15850-14, Article 15850</ispartof><rights>The Author(s) 2019</rights><rights>2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-c03d2bd12cfaf149d53008c86c2103d358d3e6edbcc53a9340903ee3ea537b2e3</citedby><cites>FETCH-LOGICAL-c474t-c03d2bd12cfaf149d53008c86c2103d358d3e6edbcc53a9340903ee3ea537b2e3</cites><orcidid>0000-0001-6246-2794 ; 0000-0001-7725-9430 ; 0000-0002-8174-046X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825138/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825138/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31676851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pisanu, Salvatore</creatorcontrib><creatorcontrib>Cacciotto, Carla</creatorcontrib><creatorcontrib>Pagnozzi, Daniela</creatorcontrib><creatorcontrib>Puggioni, Giulia Maria Grazia</creatorcontrib><creatorcontrib>Uzzau, Sergio</creatorcontrib><creatorcontrib>Ciaramella, Paolo</creatorcontrib><creatorcontrib>Guccione, Jacopo</creatorcontrib><creatorcontrib>Penati, Martina</creatorcontrib><creatorcontrib>Pollera, Claudia</creatorcontrib><creatorcontrib>Moroni, Paolo</creatorcontrib><creatorcontrib>Bronzo, Valerio</creatorcontrib><creatorcontrib>Addis, Maria Filippa</creatorcontrib><title>Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Subclinical mastitis by
Staphylococcus aureus
(SAU) and by non-aureus staphylococci (NAS) is a major issue in the water buffalo. To understand its impact on milk, 6 quarter samples with >3,000,000 cells/mL (3 SAU-positive and 3 NAS-positive) and 6 culture-negative quarter samples with <50,000 cells/mL were investigated by shotgun proteomics and label-free quantitation. A total of 1530 proteins were identified, of which 152 were significantly changed. SAU was more impacting, with 162
vs
127 differential proteins and higher abundance changes (P < 0.0005). The 119 increased proteins had mostly structural (n = 43, 28.29%) or innate immune defence functions (n = 39, 25.66%) and included vimentin, cathelicidins, histones, S100 and neutrophil granule proteins, haptoglobin, and lysozyme. The 33 decreased proteins were mainly involved in lipid metabolism (n = 13, 59.10%) and included butyrophilin, xanthine dehydrogenase/oxidase, and lipid biosynthetic enzymes. The same biological processes were significantly affected also upon STRING analysis. Cathelicidins were the most increased family, as confirmed by western immunoblotting, with a stronger reactivity in SAU mastitis. S100A8 and haptoglobin were also validated by western immunoblotting. In conclusion, we generated a detailed buffalo milk protein dataset and defined the changes occurring in SAU and NAS mastitis, with potential for improving detection (ProteomeXchange identifier PXD012355).</description><subject>631/326/2521</subject><subject>631/45/475</subject><subject>82</subject><subject>82/29</subject><subject>82/58</subject><subject>82/80</subject><subject>Animals</subject><subject>Bubalus bubalis</subject><subject>Buffalo milk</subject><subject>Buffaloes - metabolism</subject><subject>Buffaloes - microbiology</subject><subject>Butyrophilin</subject><subject>Cathelicidins</subject><subject>Cattle</subject><subject>Cell culture</subject><subject>Haptoglobin</subject><subject>Histones</subject><subject>Humanities and Social Sciences</subject><subject>Immune system</subject><subject>Immunoblotting</subject><subject>Lipid metabolism</subject><subject>Lysozyme</subject><subject>Mastitis</subject><subject>Mastitis, Bovine - metabolism</subject><subject>Mastitis, Bovine - microbiology</subject><subject>Milk</subject><subject>Milk - metabolism</subject><subject>Milk - microbiology</subject><subject>Milk Proteins - biosynthesis</subject><subject>multidisciplinary</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Quantitation</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Staphylococcal Infections - metabolism</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcal Infections - veterinary</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - metabolism</subject><subject>Vimentin</subject><subject>Xanthine dehydrogenase</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9Ustu1DAUtRCIVkN_gAWyxKYsAn7EmWSDBBWPSpWoBKwtx7lpXBx78INqvosfxOkM7cCi3lz7nnOPfayD0HNKXlPC2zexpqJrK0K7SjDS8Io9QseM1KJinLHHB_sjdBLjNSlLsK6m3VN0xGmzblpBj9Hvy-AT-NlorCflriBi43CaAM_G_sB-xDcqQcB9HkdlfYFP3-de2RxLq1QTX-EbkyYcc6-tcUYri2cVk0km4iEDTr4opqBmNc8qbMthBJ2Md7jf4q9Jbaat9dprXSRVDrAUNyyg867ad-IBzzxDT8pbIpzs6wp9__jh29nn6uLLp_OzdxeVrtd1qjThA-sHyvSoRlp3g-CEtLptNCs_OHDRDhwaGHqtBVcdr0lHOAAHJfi6Z8BX6O1Od5P7GQYNiw0rN8EsRqRXRv6LODPJK_9LNi0TlLdF4HQvEPzPDDHJ2UQN1ioHPkfJOKVNvSZ0ob78j3rtc3DF3i2LMSYaUlhsx9LBxxhgvHsMJXKJhdzFQpZYyNtYlOkVenFo427kbwgKge8IsUAlAuH-7gdk_wD0Usgf</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Pisanu, Salvatore</creator><creator>Cacciotto, Carla</creator><creator>Pagnozzi, Daniela</creator><creator>Puggioni, Giulia Maria Grazia</creator><creator>Uzzau, Sergio</creator><creator>Ciaramella, Paolo</creator><creator>Guccione, Jacopo</creator><creator>Penati, Martina</creator><creator>Pollera, Claudia</creator><creator>Moroni, Paolo</creator><creator>Bronzo, Valerio</creator><creator>Addis, Maria Filippa</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6246-2794</orcidid><orcidid>https://orcid.org/0000-0001-7725-9430</orcidid><orcidid>https://orcid.org/0000-0002-8174-046X</orcidid></search><sort><creationdate>20191101</creationdate><title>Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci</title><author>Pisanu, Salvatore ; Cacciotto, Carla ; Pagnozzi, Daniela ; Puggioni, Giulia Maria Grazia ; Uzzau, Sergio ; Ciaramella, Paolo ; Guccione, Jacopo ; Penati, Martina ; Pollera, Claudia ; Moroni, Paolo ; Bronzo, Valerio ; Addis, Maria Filippa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-c03d2bd12cfaf149d53008c86c2103d358d3e6edbcc53a9340903ee3ea537b2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>631/326/2521</topic><topic>631/45/475</topic><topic>82</topic><topic>82/29</topic><topic>82/58</topic><topic>82/80</topic><topic>Animals</topic><topic>Bubalus bubalis</topic><topic>Buffalo milk</topic><topic>Buffaloes - 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metabolism</topic><topic>Vimentin</topic><topic>Xanthine dehydrogenase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pisanu, Salvatore</creatorcontrib><creatorcontrib>Cacciotto, Carla</creatorcontrib><creatorcontrib>Pagnozzi, Daniela</creatorcontrib><creatorcontrib>Puggioni, Giulia Maria Grazia</creatorcontrib><creatorcontrib>Uzzau, Sergio</creatorcontrib><creatorcontrib>Ciaramella, Paolo</creatorcontrib><creatorcontrib>Guccione, Jacopo</creatorcontrib><creatorcontrib>Penati, Martina</creatorcontrib><creatorcontrib>Pollera, Claudia</creatorcontrib><creatorcontrib>Moroni, Paolo</creatorcontrib><creatorcontrib>Bronzo, Valerio</creatorcontrib><creatorcontrib>Addis, Maria Filippa</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pisanu, Salvatore</au><au>Cacciotto, Carla</au><au>Pagnozzi, Daniela</au><au>Puggioni, Giulia Maria Grazia</au><au>Uzzau, Sergio</au><au>Ciaramella, Paolo</au><au>Guccione, Jacopo</au><au>Penati, Martina</au><au>Pollera, Claudia</au><au>Moroni, Paolo</au><au>Bronzo, Valerio</au><au>Addis, Maria Filippa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-11-01</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>15850</spage><epage>14</epage><pages>15850-14</pages><artnum>15850</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Subclinical mastitis by
Staphylococcus aureus
(SAU) and by non-aureus staphylococci (NAS) is a major issue in the water buffalo. To understand its impact on milk, 6 quarter samples with >3,000,000 cells/mL (3 SAU-positive and 3 NAS-positive) and 6 culture-negative quarter samples with <50,000 cells/mL were investigated by shotgun proteomics and label-free quantitation. A total of 1530 proteins were identified, of which 152 were significantly changed. SAU was more impacting, with 162
vs
127 differential proteins and higher abundance changes (P < 0.0005). The 119 increased proteins had mostly structural (n = 43, 28.29%) or innate immune defence functions (n = 39, 25.66%) and included vimentin, cathelicidins, histones, S100 and neutrophil granule proteins, haptoglobin, and lysozyme. The 33 decreased proteins were mainly involved in lipid metabolism (n = 13, 59.10%) and included butyrophilin, xanthine dehydrogenase/oxidase, and lipid biosynthetic enzymes. The same biological processes were significantly affected also upon STRING analysis. Cathelicidins were the most increased family, as confirmed by western immunoblotting, with a stronger reactivity in SAU mastitis. S100A8 and haptoglobin were also validated by western immunoblotting. In conclusion, we generated a detailed buffalo milk protein dataset and defined the changes occurring in SAU and NAS mastitis, with potential for improving detection (ProteomeXchange identifier PXD012355).</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31676851</pmid><doi>10.1038/s41598-019-52063-2</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-6246-2794</orcidid><orcidid>https://orcid.org/0000-0001-7725-9430</orcidid><orcidid>https://orcid.org/0000-0002-8174-046X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 631/326/2521 631/45/475 82 82/29 82/58 82/80 Animals Bubalus bubalis Buffalo milk Buffaloes - metabolism Buffaloes - microbiology Butyrophilin Cathelicidins Cattle Cell culture Haptoglobin Histones Humanities and Social Sciences Immune system Immunoblotting Lipid metabolism Lysozyme Mastitis Mastitis, Bovine - metabolism Mastitis, Bovine - microbiology Milk Milk - metabolism Milk - microbiology Milk Proteins - biosynthesis multidisciplinary Proteins Proteomics Quantitation Science Science (multidisciplinary) Staphylococcal Infections - metabolism Staphylococcal Infections - microbiology Staphylococcal Infections - veterinary Staphylococcus aureus Staphylococcus aureus - metabolism Vimentin Xanthine dehydrogenase |
| title | Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci |
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