In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad
The study aimed to develop an efficient, rapid, and large-scale in vitro regeneration system for propagation, conservation, and restoration of an endemic and critically endangered herb, Ceropegia mohanramii . The cultures were established using nodal explants on Murashige and Skoog’s (MS) medium sup...
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| Veröffentlicht in: | Journal of Genetic Engineering and Biotechnology 2019-09, Vol.17 (1), p.2-5, Article 2 |
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| creator | Adsul, Avinash A. Chavan, Jaykumar J. Gaikwad, Nikhil B. Gurav, Rajaram V. Dixit, Ghansham B. Yadav, Shrirang R. |
| description | The study aimed to develop an efficient, rapid, and large-scale in vitro regeneration system for propagation, conservation, and restoration of an endemic and critically endangered herb,
Ceropegia mohanramii
. The cultures were established using nodal explants on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP: 1.0 mg/l). Nodal buds cultured on MS medium supplemented with BAP (2.0 mg/l) along with indole-3-butyric acid (IBA, 0.5 mg/l) resulted with production of maximum number of shoots (17.1 ± 1.2) in hundred percent of the cultures. MS medium supplemented with BAP (2.0 mg/l) along with diverse concentrations of indole-3acetic acid (IAA) promoted the in vitro flowering. In vitro regenerated shoots were transferred to one-half MS medium fortified with singular supplementation of auxins, where IBA (1.5 mg/l) served optimal for production of maximum number of roots (5.7 ± 0.6). In vitro derived plantlets were hardened under controlled conditions in a glasshouse and subsequently transferred to soil. Over 1200 saplings were transplanted to eight different localities of the Western Ghats where over 76% survival is recorded after 1 year of transplantation. |
| doi_str_mv | 10.1186/s43141-019-0003-6 |
| format | Article |
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Ceropegia mohanramii
. The cultures were established using nodal explants on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP: 1.0 mg/l). Nodal buds cultured on MS medium supplemented with BAP (2.0 mg/l) along with indole-3-butyric acid (IBA, 0.5 mg/l) resulted with production of maximum number of shoots (17.1 ± 1.2) in hundred percent of the cultures. MS medium supplemented with BAP (2.0 mg/l) along with diverse concentrations of indole-3acetic acid (IAA) promoted the in vitro flowering. In vitro regenerated shoots were transferred to one-half MS medium fortified with singular supplementation of auxins, where IBA (1.5 mg/l) served optimal for production of maximum number of roots (5.7 ± 0.6). In vitro derived plantlets were hardened under controlled conditions in a glasshouse and subsequently transferred to soil. Over 1200 saplings were transplanted to eight different localities of the Western Ghats where over 76% survival is recorded after 1 year of transplantation.</description><identifier>ISSN: 1687-157X</identifier><identifier>EISSN: 2090-5920</identifier><identifier>DOI: 10.1186/s43141-019-0003-6</identifier><identifier>PMID: 31659534</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Auxin ; Auxins ; Benzyladenine ; Biomedical Engineering and Bioengineering ; Butyric acid ; Ceropegia mohanramii ; Conservation ; Controlled conditions ; Cytokinins ; Drinking water ; Endangered ; Endangered species ; Endemic ; Engineering ; Explants ; Flowering ; Fungicides ; Greenhouses ; Indole-3-butyric acid ; Indoleacetic acid ; Laboratories ; Micropropagation ; Ornamental ; Plant growth ; Plantlets ; Propagation ; Regeneration ; Restoration ; Short Communications ; Transplantation</subject><ispartof>Journal of Genetic Engineering and Biotechnology, 2019-09, Vol.17 (1), p.2-5, Article 2</ispartof><rights>The Author(s) 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>The Author(s) 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c552t-7a88054c6b08298458832fde913ec56e7b14c0fc3712588bce2ca0bd33c0381c3</citedby><cites>FETCH-LOGICAL-c552t-7a88054c6b08298458832fde913ec56e7b14c0fc3712588bce2ca0bd33c0381c3</cites><orcidid>0000-0002-9565-0457</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821145/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821145/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27929,27930,53796,53798</link.rule.ids></links><search><creatorcontrib>Adsul, Avinash A.</creatorcontrib><creatorcontrib>Chavan, Jaykumar J.</creatorcontrib><creatorcontrib>Gaikwad, Nikhil B.</creatorcontrib><creatorcontrib>Gurav, Rajaram V.</creatorcontrib><creatorcontrib>Dixit, Ghansham B.</creatorcontrib><creatorcontrib>Yadav, Shrirang R.</creatorcontrib><title>In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad</title><title>Journal of Genetic Engineering and Biotechnology</title><addtitle>J Genet Eng Biotechnol</addtitle><description>The study aimed to develop an efficient, rapid, and large-scale in vitro regeneration system for propagation, conservation, and restoration of an endemic and critically endangered herb,
Ceropegia mohanramii
. The cultures were established using nodal explants on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP: 1.0 mg/l). Nodal buds cultured on MS medium supplemented with BAP (2.0 mg/l) along with indole-3-butyric acid (IBA, 0.5 mg/l) resulted with production of maximum number of shoots (17.1 ± 1.2) in hundred percent of the cultures. MS medium supplemented with BAP (2.0 mg/l) along with diverse concentrations of indole-3acetic acid (IAA) promoted the in vitro flowering. In vitro regenerated shoots were transferred to one-half MS medium fortified with singular supplementation of auxins, where IBA (1.5 mg/l) served optimal for production of maximum number of roots (5.7 ± 0.6). In vitro derived plantlets were hardened under controlled conditions in a glasshouse and subsequently transferred to soil. Over 1200 saplings were transplanted to eight different localities of the Western Ghats where over 76% survival is recorded after 1 year of transplantation.</description><subject>Auxin</subject><subject>Auxins</subject><subject>Benzyladenine</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Butyric acid</subject><subject>Ceropegia mohanramii</subject><subject>Conservation</subject><subject>Controlled conditions</subject><subject>Cytokinins</subject><subject>Drinking water</subject><subject>Endangered</subject><subject>Endangered species</subject><subject>Endemic</subject><subject>Engineering</subject><subject>Explants</subject><subject>Flowering</subject><subject>Fungicides</subject><subject>Greenhouses</subject><subject>Indole-3-butyric acid</subject><subject>Indoleacetic acid</subject><subject>Laboratories</subject><subject>Micropropagation</subject><subject>Ornamental</subject><subject>Plant growth</subject><subject>Plantlets</subject><subject>Propagation</subject><subject>Regeneration</subject><subject>Restoration</subject><subject>Short Communications</subject><subject>Transplantation</subject><issn>1687-157X</issn><issn>2090-5920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNp1Ustq3DAUNaWlGdJ8QHeGrp3qSrIsbwph6GMg0E0L3Ylr-dqjwZZc2ROYXT8iX9gvqSYeWrIoWgjueXB0dbLsLbBbAK3ez1KAhIJBXTDGRKFeZBvOalaUNWcvsw0oXRVQVj-uspt5PiQOK6WGEl5nVwJUWZdCbrLTzucPbokhj9STp4iLCz7HaYoB7Z7mvAsxYfMSLlDo8i3FMFHvMB_DHn3E0bnfvx7R5-RbGp3N0be5jW5xFofhdB6j7ylSm-NsB5octm-yVx0OM91c7uvs-6eP37Zfivuvn3fbu_vCliVfigq1TsGtapjmtZal1oJ3LdUgyJaKqgakZZ0VFfCENZa4Rda0QlgmNFhxne1W3zbgwUzRjRhPJqAzT4MQe4MxBR3IUMsQZS2lBS4bZZuKOGNaKZSaOtEmrw-r13RsRmot-SXi8Mz0OeLd3vThwSjNAWSZDN5dDGL4eUxbNYdwjD6933ANXEgJqkqs25XVY0rlfBeSmU3nabnBU-fS_E5V6Q-rSoskgFVgY5jnSN3fSMDMuS1mbYtJbTHnthiVNHzVzIl7_px_Uf4v-gNUiMOB</recordid><startdate>20190923</startdate><enddate>20190923</enddate><creator>Adsul, Avinash A.</creator><creator>Chavan, Jaykumar J.</creator><creator>Gaikwad, Nikhil B.</creator><creator>Gurav, Rajaram V.</creator><creator>Dixit, Ghansham B.</creator><creator>Yadav, Shrirang R.</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><general>Elsevier</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-9565-0457</orcidid></search><sort><creationdate>20190923</creationdate><title>In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad</title><author>Adsul, Avinash A. ; Chavan, Jaykumar J. ; Gaikwad, Nikhil B. ; Gurav, Rajaram V. ; Dixit, Ghansham B. ; Yadav, Shrirang R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c552t-7a88054c6b08298458832fde913ec56e7b14c0fc3712588bce2ca0bd33c0381c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Auxin</topic><topic>Auxins</topic><topic>Benzyladenine</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Butyric acid</topic><topic>Ceropegia mohanramii</topic><topic>Conservation</topic><topic>Controlled conditions</topic><topic>Cytokinins</topic><topic>Drinking water</topic><topic>Endangered</topic><topic>Endangered species</topic><topic>Endemic</topic><topic>Engineering</topic><topic>Explants</topic><topic>Flowering</topic><topic>Fungicides</topic><topic>Greenhouses</topic><topic>Indole-3-butyric acid</topic><topic>Indoleacetic acid</topic><topic>Laboratories</topic><topic>Micropropagation</topic><topic>Ornamental</topic><topic>Plant growth</topic><topic>Plantlets</topic><topic>Propagation</topic><topic>Regeneration</topic><topic>Restoration</topic><topic>Short Communications</topic><topic>Transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adsul, Avinash A.</creatorcontrib><creatorcontrib>Chavan, Jaykumar J.</creatorcontrib><creatorcontrib>Gaikwad, Nikhil B.</creatorcontrib><creatorcontrib>Gurav, Rajaram V.</creatorcontrib><creatorcontrib>Dixit, Ghansham B.</creatorcontrib><creatorcontrib>Yadav, Shrirang R.</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of Genetic Engineering and Biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adsul, Avinash A.</au><au>Chavan, Jaykumar J.</au><au>Gaikwad, Nikhil B.</au><au>Gurav, Rajaram V.</au><au>Dixit, Ghansham B.</au><au>Yadav, Shrirang R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad</atitle><jtitle>Journal of Genetic Engineering and Biotechnology</jtitle><stitle>J Genet Eng Biotechnol</stitle><date>2019-09-23</date><risdate>2019</risdate><volume>17</volume><issue>1</issue><spage>2</spage><epage>5</epage><pages>2-5</pages><artnum>2</artnum><issn>1687-157X</issn><eissn>2090-5920</eissn><abstract>The study aimed to develop an efficient, rapid, and large-scale in vitro regeneration system for propagation, conservation, and restoration of an endemic and critically endangered herb,
Ceropegia mohanramii
. The cultures were established using nodal explants on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP: 1.0 mg/l). Nodal buds cultured on MS medium supplemented with BAP (2.0 mg/l) along with indole-3-butyric acid (IBA, 0.5 mg/l) resulted with production of maximum number of shoots (17.1 ± 1.2) in hundred percent of the cultures. MS medium supplemented with BAP (2.0 mg/l) along with diverse concentrations of indole-3acetic acid (IAA) promoted the in vitro flowering. In vitro regenerated shoots were transferred to one-half MS medium fortified with singular supplementation of auxins, where IBA (1.5 mg/l) served optimal for production of maximum number of roots (5.7 ± 0.6). In vitro derived plantlets were hardened under controlled conditions in a glasshouse and subsequently transferred to soil. Over 1200 saplings were transplanted to eight different localities of the Western Ghats where over 76% survival is recorded after 1 year of transplantation.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31659534</pmid><doi>10.1186/s43141-019-0003-6</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-9565-0457</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Genetic Engineering and Biotechnology, 2019-09, Vol.17 (1), p.2-5, Article 2 |
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| source | DOAJ Directory of Open Access Journals; SpringerNature Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Auxin Auxins Benzyladenine Biomedical Engineering and Bioengineering Butyric acid Ceropegia mohanramii Conservation Controlled conditions Cytokinins Drinking water Endangered Endangered species Endemic Engineering Explants Flowering Fungicides Greenhouses Indole-3-butyric acid Indoleacetic acid Laboratories Micropropagation Ornamental Plant growth Plantlets Propagation Regeneration Restoration Short Communications Transplantation |
| title | In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad |
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