Association of VDR‐FokI and VDBP‐Thr420Lys polymorphisms with cervical spondylotic myelopathy: A case‐control study in the population of China
Background Cervical spondylotic myelopathy (CSM), a common degenerative disorder, is characterized by chronic progressive compression of the cervical spinal cord. The present case‐control study aimed to explore the potential role of VDR‐FokI and VDBP‐Thr420Lys polymorphisms in the susceptibility to...
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| creator | Song, De‐Wei Wu, Yu‐Dong Tian, Dong‐Dong |
| description | Background
Cervical spondylotic myelopathy (CSM), a common degenerative disorder, is characterized by chronic progressive compression of the cervical spinal cord. The present case‐control study aimed to explore the potential role of VDR‐FokI and VDBP‐Thr420Lys polymorphisms in the susceptibility to CSM in the Chinese population.
Methods
The study enrolled 318 CSM patients and 282 healthy individuals whose clinical data were retrospectively analyzed. Polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) analysis was used to genotype VDR‐FokI and VDBP‐Thr420Lys polymorphisms. The severity of CSM was assessed using the Japanese Orthopaedic Association (JOA) score with magnetic resonance imaging (MRI) of cervical vertebra. A nonconditional binary logistic regression model was conducted for assessing the risk factors of CSM.
Results
Patients in the CSM group had longer time duration to bend over desk working than the control group. The ff genotype and f allele frequency of VDR‐FokI were elevated in CSM patients. Elevated Ff + ff genotype and f allele frequency of VDR‐FokI might increase the risk of CSM. The VDR‐FokI polymorphism was associated with nucleus pulposus capillary invasion, necrosis, hyaline degeneration and fibrosis, genesis and hyperplasia of cartilage‐like cells, and fibrocyst in the fibrous ring. The VDR‐FokI and VDBP‐Thr420Lys genotypes conformed to Hardy‐Weinberg equilibrium which showed that VDR‐FokI and VDBP‐Thr420Lys had group representation characteristics.
Conclusion
Binary logistic regression analysis confirmed that VDR‐FokI polymorphism and the time to bend over desk working were risk factors of CSM. Our results indicate that VDR‐FokI polymorphism may be closely associated with the risk of CSM. |
| doi_str_mv | 10.1002/jcla.22669 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_24P</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6818549</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2183425467</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4489-dab767e13fca905404991660cb81677a8a99f0874078b731074181199305c8303</originalsourceid><addsrcrecordid>eNp9kcFu1DAURS0EotPChg9AltggpJRnx0lsFpWGKYWikUCosLU8jkM8OHGwk1bZ8Qks-EK-BLdTRsCClfXko_Pu00XoEYFjAkCfb7VTx5SWpbiDFgQEzyinxV20AM6rjAPJD9BhjFsA4IKU99FBDqwkUNIF-rGM0WurRut77Bv86fTDz2_fz_yXc6z6Oo0v36f5og2MwnqOePBu7nwYWhu7iK_s2GJtwqXVyuE4-L6enR-txt1snB_U2M4v8BJrFU3SaN-PwSdwnOoZ2x6PrUnGYXL7_avW9uoButcoF83D2_cIfTx7dbF6k63fvT5fLdeZZoyLrFabqqwMyRutBBQMmEjnlaA3nJRVpbgSogFeMaj4psoJVIxwQoTIodA8h_wIney8w7TpTK1NiqecHILtVJilV1b-_dPbVn72l7LkhBdMJMHTW0HwXycTR9nZqI1zqjd-ipKSvCwKmlIm9Mk_6NZPoU_nJYrnjBbshnq2o3TwMQbT7MMQkNdly-uy5U3ZCX78Z_w9-rvdBJAdcGWdmf-jkm9X6-VO-gvtuLfZ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2183425467</pqid></control><display><type>article</type><title>Association of VDR‐FokI and VDBP‐Thr420Lys polymorphisms with cervical spondylotic myelopathy: A case‐control study in the population of China</title><source>Wiley-Blackwell Open Access Titles</source><creator>Song, De‐Wei ; Wu, Yu‐Dong ; Tian, Dong‐Dong</creator><creatorcontrib>Song, De‐Wei ; Wu, Yu‐Dong ; Tian, Dong‐Dong</creatorcontrib><description>Background
Cervical spondylotic myelopathy (CSM), a common degenerative disorder, is characterized by chronic progressive compression of the cervical spinal cord. The present case‐control study aimed to explore the potential role of VDR‐FokI and VDBP‐Thr420Lys polymorphisms in the susceptibility to CSM in the Chinese population.
Methods
The study enrolled 318 CSM patients and 282 healthy individuals whose clinical data were retrospectively analyzed. Polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) analysis was used to genotype VDR‐FokI and VDBP‐Thr420Lys polymorphisms. The severity of CSM was assessed using the Japanese Orthopaedic Association (JOA) score with magnetic resonance imaging (MRI) of cervical vertebra. A nonconditional binary logistic regression model was conducted for assessing the risk factors of CSM.
Results
Patients in the CSM group had longer time duration to bend over desk working than the control group. The ff genotype and f allele frequency of VDR‐FokI were elevated in CSM patients. Elevated Ff + ff genotype and f allele frequency of VDR‐FokI might increase the risk of CSM. The VDR‐FokI polymorphism was associated with nucleus pulposus capillary invasion, necrosis, hyaline degeneration and fibrosis, genesis and hyperplasia of cartilage‐like cells, and fibrocyst in the fibrous ring. The VDR‐FokI and VDBP‐Thr420Lys genotypes conformed to Hardy‐Weinberg equilibrium which showed that VDR‐FokI and VDBP‐Thr420Lys had group representation characteristics.
Conclusion
Binary logistic regression analysis confirmed that VDR‐FokI polymorphism and the time to bend over desk working were risk factors of CSM. Our results indicate that VDR‐FokI polymorphism may be closely associated with the risk of CSM.</description><identifier>ISSN: 0887-8013</identifier><identifier>EISSN: 1098-2825</identifier><identifier>DOI: 10.1002/jcla.22669</identifier><identifier>PMID: 30461062</identifier><language>eng</language><publisher>United States: John Wiley & Sons, Inc</publisher><subject>Aged ; Alleles ; Cartilage ; Case-Control Studies ; Central nervous system diseases ; cervical spondylotic myelopathy ; China - epidemiology ; Compression ; Data processing ; Degeneration ; Female ; Fibrosis ; FokI ; Gene frequency ; Gene polymorphism ; Genetic Predisposition to Disease - epidemiology ; Genetic Predisposition to Disease - genetics ; Genotype ; Genotype & phenotype ; Humans ; Hyperplasia ; Intervertebral discs ; Magnetic resonance imaging ; Male ; Middle Aged ; NMR ; Nuclear magnetic resonance ; Nucleus pulposus ; Polymerase chain reaction ; Polymorphism ; Polymorphism, Genetic ; Population studies ; Receptors, Calcitriol - genetics ; Restriction fragment length polymorphism ; Retrospective Studies ; Risk factors ; Spinal cord ; Spinal Cord Diseases - epidemiology ; Spinal Cord Diseases - genetics ; Spondylosis - epidemiology ; Spondylosis - genetics ; Thr420Lys ; VDBP ; VDR ; Vertebrae</subject><ispartof>Journal of clinical laboratory analysis, 2019-02, Vol.33 (2), p.e22669-n/a</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><rights>Copyright © 2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4489-dab767e13fca905404991660cb81677a8a99f0874078b731074181199305c8303</citedby><cites>FETCH-LOGICAL-c4489-dab767e13fca905404991660cb81677a8a99f0874078b731074181199305c8303</cites><orcidid>0000-0002-0427-6253</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6818549/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6818549/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1002%2Fjcla.22669$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30461062$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, De‐Wei</creatorcontrib><creatorcontrib>Wu, Yu‐Dong</creatorcontrib><creatorcontrib>Tian, Dong‐Dong</creatorcontrib><title>Association of VDR‐FokI and VDBP‐Thr420Lys polymorphisms with cervical spondylotic myelopathy: A case‐control study in the population of China</title><title>Journal of clinical laboratory analysis</title><addtitle>J Clin Lab Anal</addtitle><description>Background
Cervical spondylotic myelopathy (CSM), a common degenerative disorder, is characterized by chronic progressive compression of the cervical spinal cord. The present case‐control study aimed to explore the potential role of VDR‐FokI and VDBP‐Thr420Lys polymorphisms in the susceptibility to CSM in the Chinese population.
Methods
The study enrolled 318 CSM patients and 282 healthy individuals whose clinical data were retrospectively analyzed. Polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) analysis was used to genotype VDR‐FokI and VDBP‐Thr420Lys polymorphisms. The severity of CSM was assessed using the Japanese Orthopaedic Association (JOA) score with magnetic resonance imaging (MRI) of cervical vertebra. A nonconditional binary logistic regression model was conducted for assessing the risk factors of CSM.
Results
Patients in the CSM group had longer time duration to bend over desk working than the control group. The ff genotype and f allele frequency of VDR‐FokI were elevated in CSM patients. Elevated Ff + ff genotype and f allele frequency of VDR‐FokI might increase the risk of CSM. The VDR‐FokI polymorphism was associated with nucleus pulposus capillary invasion, necrosis, hyaline degeneration and fibrosis, genesis and hyperplasia of cartilage‐like cells, and fibrocyst in the fibrous ring. The VDR‐FokI and VDBP‐Thr420Lys genotypes conformed to Hardy‐Weinberg equilibrium which showed that VDR‐FokI and VDBP‐Thr420Lys had group representation characteristics.
Conclusion
Binary logistic regression analysis confirmed that VDR‐FokI polymorphism and the time to bend over desk working were risk factors of CSM. Our results indicate that VDR‐FokI polymorphism may be closely associated with the risk of CSM.</description><subject>Aged</subject><subject>Alleles</subject><subject>Cartilage</subject><subject>Case-Control Studies</subject><subject>Central nervous system diseases</subject><subject>cervical spondylotic myelopathy</subject><subject>China - epidemiology</subject><subject>Compression</subject><subject>Data processing</subject><subject>Degeneration</subject><subject>Female</subject><subject>Fibrosis</subject><subject>FokI</subject><subject>Gene frequency</subject><subject>Gene polymorphism</subject><subject>Genetic Predisposition to Disease - epidemiology</subject><subject>Genetic Predisposition to Disease - genetics</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Humans</subject><subject>Hyperplasia</subject><subject>Intervertebral discs</subject><subject>Magnetic resonance imaging</subject><subject>Male</subject><subject>Middle Aged</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Nucleus pulposus</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Polymorphism, Genetic</subject><subject>Population studies</subject><subject>Receptors, Calcitriol - genetics</subject><subject>Restriction fragment length polymorphism</subject><subject>Retrospective Studies</subject><subject>Risk factors</subject><subject>Spinal cord</subject><subject>Spinal Cord Diseases - epidemiology</subject><subject>Spinal Cord Diseases - genetics</subject><subject>Spondylosis - epidemiology</subject><subject>Spondylosis - genetics</subject><subject>Thr420Lys</subject><subject>VDBP</subject><subject>VDR</subject><subject>Vertebrae</subject><issn>0887-8013</issn><issn>1098-2825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAURS0EotPChg9AltggpJRnx0lsFpWGKYWikUCosLU8jkM8OHGwk1bZ8Qks-EK-BLdTRsCClfXko_Pu00XoEYFjAkCfb7VTx5SWpbiDFgQEzyinxV20AM6rjAPJD9BhjFsA4IKU99FBDqwkUNIF-rGM0WurRut77Bv86fTDz2_fz_yXc6z6Oo0v36f5og2MwnqOePBu7nwYWhu7iK_s2GJtwqXVyuE4-L6enR-txt1snB_U2M4v8BJrFU3SaN-PwSdwnOoZ2x6PrUnGYXL7_avW9uoButcoF83D2_cIfTx7dbF6k63fvT5fLdeZZoyLrFabqqwMyRutBBQMmEjnlaA3nJRVpbgSogFeMaj4psoJVIxwQoTIodA8h_wIney8w7TpTK1NiqecHILtVJilV1b-_dPbVn72l7LkhBdMJMHTW0HwXycTR9nZqI1zqjd-ipKSvCwKmlIm9Mk_6NZPoU_nJYrnjBbshnq2o3TwMQbT7MMQkNdly-uy5U3ZCX78Z_w9-rvdBJAdcGWdmf-jkm9X6-VO-gvtuLfZ</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Song, De‐Wei</creator><creator>Wu, Yu‐Dong</creator><creator>Tian, Dong‐Dong</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0427-6253</orcidid></search><sort><creationdate>201902</creationdate><title>Association of VDR‐FokI and VDBP‐Thr420Lys polymorphisms with cervical spondylotic myelopathy: A case‐control study in the population of China</title><author>Song, De‐Wei ; Wu, Yu‐Dong ; Tian, Dong‐Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4489-dab767e13fca905404991660cb81677a8a99f0874078b731074181199305c8303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aged</topic><topic>Alleles</topic><topic>Cartilage</topic><topic>Case-Control Studies</topic><topic>Central nervous system diseases</topic><topic>cervical spondylotic myelopathy</topic><topic>China - epidemiology</topic><topic>Compression</topic><topic>Data processing</topic><topic>Degeneration</topic><topic>Female</topic><topic>Fibrosis</topic><topic>FokI</topic><topic>Gene frequency</topic><topic>Gene polymorphism</topic><topic>Genetic Predisposition to Disease - epidemiology</topic><topic>Genetic Predisposition to Disease - genetics</topic><topic>Genotype</topic><topic>Genotype & phenotype</topic><topic>Humans</topic><topic>Hyperplasia</topic><topic>Intervertebral discs</topic><topic>Magnetic resonance imaging</topic><topic>Male</topic><topic>Middle Aged</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Nucleus pulposus</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism</topic><topic>Polymorphism, Genetic</topic><topic>Population studies</topic><topic>Receptors, Calcitriol - genetics</topic><topic>Restriction fragment length polymorphism</topic><topic>Retrospective Studies</topic><topic>Risk factors</topic><topic>Spinal cord</topic><topic>Spinal Cord Diseases - epidemiology</topic><topic>Spinal Cord Diseases - genetics</topic><topic>Spondylosis - epidemiology</topic><topic>Spondylosis - genetics</topic><topic>Thr420Lys</topic><topic>VDBP</topic><topic>VDR</topic><topic>Vertebrae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, De‐Wei</creatorcontrib><creatorcontrib>Wu, Yu‐Dong</creatorcontrib><creatorcontrib>Tian, Dong‐Dong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical laboratory analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Song, De‐Wei</au><au>Wu, Yu‐Dong</au><au>Tian, Dong‐Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of VDR‐FokI and VDBP‐Thr420Lys polymorphisms with cervical spondylotic myelopathy: A case‐control study in the population of China</atitle><jtitle>Journal of clinical laboratory analysis</jtitle><addtitle>J Clin Lab Anal</addtitle><date>2019-02</date><risdate>2019</risdate><volume>33</volume><issue>2</issue><spage>e22669</spage><epage>n/a</epage><pages>e22669-n/a</pages><issn>0887-8013</issn><eissn>1098-2825</eissn><abstract>Background
Cervical spondylotic myelopathy (CSM), a common degenerative disorder, is characterized by chronic progressive compression of the cervical spinal cord. The present case‐control study aimed to explore the potential role of VDR‐FokI and VDBP‐Thr420Lys polymorphisms in the susceptibility to CSM in the Chinese population.
Methods
The study enrolled 318 CSM patients and 282 healthy individuals whose clinical data were retrospectively analyzed. Polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) analysis was used to genotype VDR‐FokI and VDBP‐Thr420Lys polymorphisms. The severity of CSM was assessed using the Japanese Orthopaedic Association (JOA) score with magnetic resonance imaging (MRI) of cervical vertebra. A nonconditional binary logistic regression model was conducted for assessing the risk factors of CSM.
Results
Patients in the CSM group had longer time duration to bend over desk working than the control group. The ff genotype and f allele frequency of VDR‐FokI were elevated in CSM patients. Elevated Ff + ff genotype and f allele frequency of VDR‐FokI might increase the risk of CSM. The VDR‐FokI polymorphism was associated with nucleus pulposus capillary invasion, necrosis, hyaline degeneration and fibrosis, genesis and hyperplasia of cartilage‐like cells, and fibrocyst in the fibrous ring. The VDR‐FokI and VDBP‐Thr420Lys genotypes conformed to Hardy‐Weinberg equilibrium which showed that VDR‐FokI and VDBP‐Thr420Lys had group representation characteristics.
Conclusion
Binary logistic regression analysis confirmed that VDR‐FokI polymorphism and the time to bend over desk working were risk factors of CSM. Our results indicate that VDR‐FokI polymorphism may be closely associated with the risk of CSM.</abstract><cop>United States</cop><pub>John Wiley & Sons, Inc</pub><pmid>30461062</pmid><doi>10.1002/jcla.22669</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-0427-6253</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0887-8013 |
| ispartof | Journal of clinical laboratory analysis, 2019-02, Vol.33 (2), p.e22669-n/a |
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| source | Wiley-Blackwell Open Access Titles |
| subjects | Aged Alleles Cartilage Case-Control Studies Central nervous system diseases cervical spondylotic myelopathy China - epidemiology Compression Data processing Degeneration Female Fibrosis FokI Gene frequency Gene polymorphism Genetic Predisposition to Disease - epidemiology Genetic Predisposition to Disease - genetics Genotype Genotype & phenotype Humans Hyperplasia Intervertebral discs Magnetic resonance imaging Male Middle Aged NMR Nuclear magnetic resonance Nucleus pulposus Polymerase chain reaction Polymorphism Polymorphism, Genetic Population studies Receptors, Calcitriol - genetics Restriction fragment length polymorphism Retrospective Studies Risk factors Spinal cord Spinal Cord Diseases - epidemiology Spinal Cord Diseases - genetics Spondylosis - epidemiology Spondylosis - genetics Thr420Lys VDBP VDR Vertebrae |
| title | Association of VDR‐FokI and VDBP‐Thr420Lys polymorphisms with cervical spondylotic myelopathy: A case‐control study in the population of China |
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