The Interactome analysis of the Respiratory Syncytial Virus protein M2-1 suggests a new role in viral mRNA metabolism post-transcription
Human respiratory syncytial virus (RSV) is a globally prevalent negative-stranded RNA virus, which can cause life-threatening respiratory infections in young children, elderly people and immunocompromised patients. Its transcription termination factor M2-1 plays an essential role in viral transcript...
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| creator | Bouillier, Camille Cosentino, Gina Léger, Thibaut Rincheval, Vincent Richard, Charles-Adrien Desquesnes, Aurore Sitterlin, Delphine Blouquit-Laye, Sabine Eléouët, Jean-Francois Gault, Elyanne Rameix-Welti, Marie-Anne |
| description | Human respiratory syncytial virus (RSV) is a globally prevalent negative-stranded RNA virus, which can cause life-threatening respiratory infections in young children, elderly people and immunocompromised patients. Its transcription termination factor M2-1 plays an essential role in viral transcription, but the mechanisms underpinning its function are still unclear. We investigated the cellular interactome of M2-1 using green fluorescent protein (GFP)-trap immunoprecipitation on RSV infected cells coupled with mass spectrometry analysis. We identified 137 potential cellular partners of M2-1, among which many proteins associated with mRNA metabolism, and particularly mRNA maturation, translation and stabilization. Among these, the cytoplasmic polyA-binding protein 1 (PABPC1), a candidate with a major role in both translation and mRNA stabilization, was confirmed to interact with M2-1 using protein complementation assay and specific immunoprecipitation. PABPC1 was also shown to colocalize with M2-1 from its accumulation in inclusion bodies associated granules (IBAGs) to its liberation in the cytoplasm. Altogether, these results strongly suggest that M2-1 interacts with viral mRNA and mRNA metabolism factors from transcription to translation, and imply that M2-1 may have an additional role in the fate of viral mRNA downstream of transcription. |
| doi_str_mv | 10.1038/s41598-019-51746-0 |
| format | Article |
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Its transcription termination factor M2-1 plays an essential role in viral transcription, but the mechanisms underpinning its function are still unclear. We investigated the cellular interactome of M2-1 using green fluorescent protein (GFP)-trap immunoprecipitation on RSV infected cells coupled with mass spectrometry analysis. We identified 137 potential cellular partners of M2-1, among which many proteins associated with mRNA metabolism, and particularly mRNA maturation, translation and stabilization. Among these, the cytoplasmic polyA-binding protein 1 (PABPC1), a candidate with a major role in both translation and mRNA stabilization, was confirmed to interact with M2-1 using protein complementation assay and specific immunoprecipitation. PABPC1 was also shown to colocalize with M2-1 from its accumulation in inclusion bodies associated granules (IBAGs) to its liberation in the cytoplasm. Altogether, these results strongly suggest that M2-1 interacts with viral mRNA and mRNA metabolism factors from transcription to translation, and imply that M2-1 may have an additional role in the fate of viral mRNA downstream of transcription.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-51746-0</identifier><identifier>PMID: 31649314</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14/19 ; 14/35 ; 42/109 ; 42/44 ; 42/89 ; 631/326/596/2557 ; 631/337/475 ; 82/111 ; 82/58 ; Cytoplasm ; Geriatrics ; Green fluorescent protein ; Humanities and Social Sciences ; Humans ; Immunocompromised hosts ; Immunoprecipitation ; Inclusion bodies ; Life Sciences ; Mass spectrometry ; Mass spectroscopy ; Metabolism ; multidisciplinary ; Post-transcription ; Protein Interaction Maps - physiology ; Proteins ; Respiratory syncytial virus ; Respiratory Syncytial Virus Infections - virology ; Respiratory Syncytial Virus, Human - metabolism ; RNA viruses ; RNA, Viral - metabolism ; Santé publique et épidémiologie ; Science ; Science (multidisciplinary) ; Transcription factors ; Transcription termination ; Translation ; Viral Proteins - metabolism</subject><ispartof>Scientific reports, 2019-10, Vol.9 (1), p.15258-13, Article 15258</ispartof><rights>The Author(s) 2019</rights><rights>2019. 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Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bouillier, Camille</au><au>Cosentino, Gina</au><au>Léger, Thibaut</au><au>Rincheval, Vincent</au><au>Richard, Charles-Adrien</au><au>Desquesnes, Aurore</au><au>Sitterlin, Delphine</au><au>Blouquit-Laye, Sabine</au><au>Eléouët, Jean-Francois</au><au>Gault, Elyanne</au><au>Rameix-Welti, Marie-Anne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Interactome analysis of the Respiratory Syncytial Virus protein M2-1 suggests a new role in viral mRNA metabolism post-transcription</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-10-24</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>15258</spage><epage>13</epage><pages>15258-13</pages><artnum>15258</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Human respiratory syncytial virus (RSV) is a globally prevalent negative-stranded RNA virus, which can cause life-threatening respiratory infections in young children, elderly people and immunocompromised patients. Its transcription termination factor M2-1 plays an essential role in viral transcription, but the mechanisms underpinning its function are still unclear. We investigated the cellular interactome of M2-1 using green fluorescent protein (GFP)-trap immunoprecipitation on RSV infected cells coupled with mass spectrometry analysis. We identified 137 potential cellular partners of M2-1, among which many proteins associated with mRNA metabolism, and particularly mRNA maturation, translation and stabilization. Among these, the cytoplasmic polyA-binding protein 1 (PABPC1), a candidate with a major role in both translation and mRNA stabilization, was confirmed to interact with M2-1 using protein complementation assay and specific immunoprecipitation. PABPC1 was also shown to colocalize with M2-1 from its accumulation in inclusion bodies associated granules (IBAGs) to its liberation in the cytoplasm. Altogether, these results strongly suggest that M2-1 interacts with viral mRNA and mRNA metabolism factors from transcription to translation, and imply that M2-1 may have an additional role in the fate of viral mRNA downstream of transcription.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31649314</pmid><doi>10.1038/s41598-019-51746-0</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-5901-3856</orcidid><orcidid>https://orcid.org/0000-0001-5306-2297</orcidid><orcidid>https://orcid.org/0000-0002-6304-3433</orcidid><orcidid>https://orcid.org/0000-0002-7361-4885</orcidid><orcidid>https://orcid.org/0000-0002-2114-9926</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 14/19 14/35 42/109 42/44 42/89 631/326/596/2557 631/337/475 82/111 82/58 Cytoplasm Geriatrics Green fluorescent protein Humanities and Social Sciences Humans Immunocompromised hosts Immunoprecipitation Inclusion bodies Life Sciences Mass spectrometry Mass spectroscopy Metabolism multidisciplinary Post-transcription Protein Interaction Maps - physiology Proteins Respiratory syncytial virus Respiratory Syncytial Virus Infections - virology Respiratory Syncytial Virus, Human - metabolism RNA viruses RNA, Viral - metabolism Santé publique et épidémiologie Science Science (multidisciplinary) Transcription factors Transcription termination Translation Viral Proteins - metabolism |
| title | The Interactome analysis of the Respiratory Syncytial Virus protein M2-1 suggests a new role in viral mRNA metabolism post-transcription |
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