Significance of cerebrospinal fluid adenosine deaminase isoenzymes in tuberculous (TB) meningitis
Adenosine deaminase (ADA) exists as two isoenzymes, ADA1 and ADA2. It appears that the ADA2 isoenzyme originates mainly from monocytes and macrophages. In tuberculous pleural effusions most of the ADA activity consists of ADA2. The aim of this prospective study was to analyse ADA isoenzymes in the C...
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| Veröffentlicht in: | Journal of clinical laboratory analysis 2001, Vol.15 (5), p.236-238 |
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| creator | Schutte, C.-M. Ungerer, J.P.J. du Plessis, H. van der Meyden, C.H. |
| description | Adenosine deaminase (ADA) exists as two isoenzymes, ADA1 and ADA2. It appears that the ADA2 isoenzyme originates mainly from monocytes and macrophages. In tuberculous pleural effusions most of the ADA activity consists of ADA2. The aim of this prospective study was to analyse ADA isoenzymes in the CSF of patients with meningitis to investigate whether the expected rise of the ADA2 isoenzyme would occur in tuberculous meningitis. ADA isoenzyme analysis was performed on the CSF of 15 patients with tuberculous and 11 patients with bacterial meningitis by an automated kinetic enzyme coupled assay in the presence and absence of a specific ADA inhibitor. The ratio of ADA2/ADATotal was >0.8 in 14/15 patients with tuberculous meningitis. In bacterial meningitis the ratio was ≥0.8 in 10/11 patients. The ADA2 isoenzyme is the major contributor to increased ADA activity in the CSF of patients with tuberculous meningitis, probably reflecting the monocyte–macrophage origin of the ADA. J. Clin. Lab. Anal. 15:236–238, 2001. © 2001 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jcla.1033 |
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It appears that the ADA2 isoenzyme originates mainly from monocytes and macrophages. In tuberculous pleural effusions most of the ADA activity consists of ADA2. The aim of this prospective study was to analyse ADA isoenzymes in the CSF of patients with meningitis to investigate whether the expected rise of the ADA2 isoenzyme would occur in tuberculous meningitis. ADA isoenzyme analysis was performed on the CSF of 15 patients with tuberculous and 11 patients with bacterial meningitis by an automated kinetic enzyme coupled assay in the presence and absence of a specific ADA inhibitor. The ratio of ADA2/ADATotal was >0.8 in 14/15 patients with tuberculous meningitis. In bacterial meningitis the ratio was ≥0.8 in 10/11 patients. The ADA2 isoenzyme is the major contributor to increased ADA activity in the CSF of patients with tuberculous meningitis, probably reflecting the monocyte–macrophage origin of the ADA. J. Clin. Lab. Anal. 15:236–238, 2001. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 0887-8013</identifier><identifier>EISSN: 1098-2825</identifier><identifier>DOI: 10.1002/jcla.1033</identifier><identifier>PMID: 11574950</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Adenosine Deaminase - cerebrospinal fluid ; Adolescent ; Adult ; chronic meningitis ; diagnosis ; Female ; Humans ; Isoenzymes - cerebrospinal fluid ; Male ; Middle Aged ; Original ; tuberculosis ; Tuberculosis, Meningeal - cerebrospinal fluid ; Tuberculosis, Meningeal - enzymology</subject><ispartof>Journal of clinical laboratory analysis, 2001, Vol.15 (5), p.236-238</ispartof><rights>Copyright © 2001 Wiley‐Liss, Inc.</rights><rights>Copyright 2001 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4493-3dc74b2f09bf686b3dfd95b9b56d07ab799ee68f8166da3eb91541b4ec865a303</citedby><cites>FETCH-LOGICAL-c4493-3dc74b2f09bf686b3dfd95b9b56d07ab799ee68f8166da3eb91541b4ec865a303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6807738/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6807738/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,4010,27900,27901,27902,45550,45551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11574950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schutte, C.-M.</creatorcontrib><creatorcontrib>Ungerer, J.P.J.</creatorcontrib><creatorcontrib>du Plessis, H.</creatorcontrib><creatorcontrib>van der Meyden, C.H.</creatorcontrib><title>Significance of cerebrospinal fluid adenosine deaminase isoenzymes in tuberculous (TB) meningitis</title><title>Journal of clinical laboratory analysis</title><addtitle>J. Clin. Lab. Anal</addtitle><description>Adenosine deaminase (ADA) exists as two isoenzymes, ADA1 and ADA2. It appears that the ADA2 isoenzyme originates mainly from monocytes and macrophages. In tuberculous pleural effusions most of the ADA activity consists of ADA2. The aim of this prospective study was to analyse ADA isoenzymes in the CSF of patients with meningitis to investigate whether the expected rise of the ADA2 isoenzyme would occur in tuberculous meningitis. ADA isoenzyme analysis was performed on the CSF of 15 patients with tuberculous and 11 patients with bacterial meningitis by an automated kinetic enzyme coupled assay in the presence and absence of a specific ADA inhibitor. The ratio of ADA2/ADATotal was >0.8 in 14/15 patients with tuberculous meningitis. In bacterial meningitis the ratio was ≥0.8 in 10/11 patients. The ADA2 isoenzyme is the major contributor to increased ADA activity in the CSF of patients with tuberculous meningitis, probably reflecting the monocyte–macrophage origin of the ADA. J. Clin. Lab. Anal. 15:236–238, 2001. © 2001 Wiley‐Liss, Inc.</description><subject>Adenosine Deaminase - cerebrospinal fluid</subject><subject>Adolescent</subject><subject>Adult</subject><subject>chronic meningitis</subject><subject>diagnosis</subject><subject>Female</subject><subject>Humans</subject><subject>Isoenzymes - cerebrospinal fluid</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Original</subject><subject>tuberculosis</subject><subject>Tuberculosis, Meningeal - cerebrospinal fluid</subject><subject>Tuberculosis, Meningeal - enzymology</subject><issn>0887-8013</issn><issn>1098-2825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1v1DAQhi1ERZfCgT-AfEL0kNaO468LUruipbAsEhTBzbKdyeKSOIudFJZf36x2VeDAyZbmmWfGfhF6RskJJaQ8vfGtnW6MPUAzSrQqSlXyh2hGlJKFIpQdosc53xBClKbiETqklMtKczJD9lNYxdAEb6MH3DfYQwKX-rwO0ba4acdQY1tD7HOIgGuw3VTIgEPuIf7edJBxiHgYHSQ_tv2Y8cvr82PcQQxxFYaQn6CDxrYZnu7PI_T54vX1_E2x-HB5NT9bFL6qNCtY7WXlyoZo1wglHKubWnOnHRc1kdZJrQGEahQVorYMnKa8oq4CrwS3jLAj9GrnXY-ug9pDHJJtzTqFzqaN6W0w_1Zi-GZW_a0RikjJ1CR4sRek_scIeTBdyB7a1kaY3mUkLQnncjvpeAf66Z9yguZ-CCVmG4jZBmK2gUzs87-3-kPuE5iA0x3wM7Sw-b_JvJ0vzvbKYtcR8gC_7jts-m6EZJKbL8tLw_jy60fxfmnesTusFqeU</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Schutte, C.-M.</creator><creator>Ungerer, J.P.J.</creator><creator>du Plessis, H.</creator><creator>van der Meyden, C.H.</creator><general>John Wiley & Sons, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2001</creationdate><title>Significance of cerebrospinal fluid adenosine deaminase isoenzymes in tuberculous (TB) meningitis</title><author>Schutte, C.-M. ; Ungerer, J.P.J. ; du Plessis, H. ; van der Meyden, C.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4493-3dc74b2f09bf686b3dfd95b9b56d07ab799ee68f8166da3eb91541b4ec865a303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adenosine Deaminase - cerebrospinal fluid</topic><topic>Adolescent</topic><topic>Adult</topic><topic>chronic meningitis</topic><topic>diagnosis</topic><topic>Female</topic><topic>Humans</topic><topic>Isoenzymes - cerebrospinal fluid</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Original</topic><topic>tuberculosis</topic><topic>Tuberculosis, Meningeal - cerebrospinal fluid</topic><topic>Tuberculosis, Meningeal - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schutte, C.-M.</creatorcontrib><creatorcontrib>Ungerer, J.P.J.</creatorcontrib><creatorcontrib>du Plessis, H.</creatorcontrib><creatorcontrib>van der Meyden, C.H.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical laboratory analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schutte, C.-M.</au><au>Ungerer, J.P.J.</au><au>du Plessis, H.</au><au>van der Meyden, C.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Significance of cerebrospinal fluid adenosine deaminase isoenzymes in tuberculous (TB) meningitis</atitle><jtitle>Journal of clinical laboratory analysis</jtitle><addtitle>J. Clin. Lab. Anal</addtitle><date>2001</date><risdate>2001</risdate><volume>15</volume><issue>5</issue><spage>236</spage><epage>238</epage><pages>236-238</pages><issn>0887-8013</issn><eissn>1098-2825</eissn><abstract>Adenosine deaminase (ADA) exists as two isoenzymes, ADA1 and ADA2. It appears that the ADA2 isoenzyme originates mainly from monocytes and macrophages. In tuberculous pleural effusions most of the ADA activity consists of ADA2. The aim of this prospective study was to analyse ADA isoenzymes in the CSF of patients with meningitis to investigate whether the expected rise of the ADA2 isoenzyme would occur in tuberculous meningitis. ADA isoenzyme analysis was performed on the CSF of 15 patients with tuberculous and 11 patients with bacterial meningitis by an automated kinetic enzyme coupled assay in the presence and absence of a specific ADA inhibitor. The ratio of ADA2/ADATotal was >0.8 in 14/15 patients with tuberculous meningitis. In bacterial meningitis the ratio was ≥0.8 in 10/11 patients. The ADA2 isoenzyme is the major contributor to increased ADA activity in the CSF of patients with tuberculous meningitis, probably reflecting the monocyte–macrophage origin of the ADA. J. Clin. Lab. Anal. 15:236–238, 2001. © 2001 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11574950</pmid><doi>10.1002/jcla.1033</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adenosine Deaminase - cerebrospinal fluid Adolescent Adult chronic meningitis diagnosis Female Humans Isoenzymes - cerebrospinal fluid Male Middle Aged Original tuberculosis Tuberculosis, Meningeal - cerebrospinal fluid Tuberculosis, Meningeal - enzymology |
| title | Significance of cerebrospinal fluid adenosine deaminase isoenzymes in tuberculous (TB) meningitis |
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