Ribonucleic Acid Extraction From Archival Formalin Fixed Paraffin Embedded Myocardial Tissues for Gene Expression and Pathogen Detection

Introduction Archival tissue samples preserved in formalin are a great source of treasure for biomedical research and diagnostics. Formalin, though is a good preservative, causes the modification of nucleic acid limiting the application of fixed tissues. The present study evaluated three methods of...

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Veröffentlicht in:	Journal of clinical laboratory analysis 2012-07, Vol.26 (4), p.279-285
Hauptverfasser:	Sharma, Mirnalini, Mishra, Baijayantimala, Vandana, Saikia, Uma N., Bahl, Ajay, Ratho, Radha K., Talwar, Kewal K.
Format:	Artikel
Sprache:	eng
Schlagworte:	archival tissues Biomedical research Dilated cardiomyopathy Enterovirus Enterovirus - genetics Enterovirus - isolation & purification Formaldehyde formalin-fixed paraffin-embedded Gene expression Gene Expression Profiling - methods Genome, Viral Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - genetics Heart - virology Humans Myocardium - chemistry Paraffin Embedding - methods Pathology Real-Time Polymerase Chain Reaction Ribonucleic acid RNA RNA - isolation & purification RNA extraction Statistics, Nonparametric virus detection
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