The Use of Factorial Design, Image Analysis, and an Efficiency Calculation for Multiplex PCR Optimization

Background The quality of multiplex polymerase chain reaction (PCR) assays depends on several factors. Therefore, it is important to establish the optimal conditions to achieve efficient amplification. The objective of this study was to implement a 5 × 4 factorial design combined with image analysis...

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Veröffentlicht in:Journal of clinical laboratory analysis 2013-05, Vol.27 (3), p.249-252
Hauptverfasser: Camacho, José Luis Villarreal, Torres, Evelyn Mendoza, Cadena, Christian, Prieto, Julieth, Prieto, Lourdes Luz Varela, Torregroza, Daniel Antonio Villanueva
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Sprache:eng
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