CRISPR/Cas9-Assisted Seamless Genome Editing in Lactobacillus plantarum and Its Application in N -Acetylglucosamine Production

is a potential starter and health-promoting probiotic bacterium. Effective, precise, and diverse genome editing of without introducing exogenous genes or plasmids is of great importance. In this study, CRISPR/Cas9-assisted double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) recombineering wa...

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