Specialized membrane domains for water transport in glial cells: high-resolution immunogold cytochemistry of aquaporin-4 in rat brain
Membrane water transport is critically involved in brain volume homeostasis and in the pathogenesis of brain edema. The cDNA encoding aquaporin-4 (AQP4) water channel protein was recently isolated from rat brain. We used immunocytochemistry and high-resolution immunogold electron microscopy to ident...
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| Veröffentlicht in: | The Journal of neuroscience 1997-01, Vol.17 (1), p.171-180 |
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| creator | Nielsen, S Nagelhus, E A Amiry-Moghaddam, M Bourque, C Agre, P Ottersen, O P |
| description | Membrane water transport is critically involved in brain volume homeostasis and in the pathogenesis of brain edema. The cDNA encoding aquaporin-4 (AQP4) water channel protein was recently isolated from rat brain. We used immunocytochemistry and high-resolution immunogold electron microscopy to identify the cells and membrane domains that mediate water flux through AQP4. The AQP4 protein is abundant in glial cells bordering the subarachnoidal space, ventricles, and blood vessels. AQP4 is also abundant in osmosensory areas, including the supraoptic nucleus and subfornical organ. Immunogold analysis demonstrated that AQP4 is restricted to glial membranes and to subpopulations of ependymal cells. AQP4 is particularly strongly expressed in glial membranes that are in direct contact with capillaries and pia. The highly polarized AQP4 expression indicates that these cells are equipped with specific membrane domains that are specialized for water transport, thereby mediating the flow of water between glial cells and the cavities filled with CSF and the intravascular space. |
| doi_str_mv | 10.1523/jneurosci.17-01-00171.1997 |
| format | Article |
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The cDNA encoding aquaporin-4 (AQP4) water channel protein was recently isolated from rat brain. We used immunocytochemistry and high-resolution immunogold electron microscopy to identify the cells and membrane domains that mediate water flux through AQP4. The AQP4 protein is abundant in glial cells bordering the subarachnoidal space, ventricles, and blood vessels. AQP4 is also abundant in osmosensory areas, including the supraoptic nucleus and subfornical organ. Immunogold analysis demonstrated that AQP4 is restricted to glial membranes and to subpopulations of ependymal cells. AQP4 is particularly strongly expressed in glial membranes that are in direct contact with capillaries and pia. The highly polarized AQP4 expression indicates that these cells are equipped with specific membrane domains that are specialized for water transport, thereby mediating the flow of water between glial cells and the cavities filled with CSF and the intravascular space.</description><identifier>ISSN: 0270-6474</identifier><identifier>EISSN: 1529-2401</identifier><identifier>DOI: 10.1523/jneurosci.17-01-00171.1997</identifier><identifier>PMID: 8987746</identifier><language>eng</language><publisher>United States: Society for Neuroscience</publisher><subject>Animals ; Aquaporin 4 ; Aquaporins ; Astrocytes - metabolism ; Biological Transport ; Brain - metabolism ; Brain - ultrastructure ; Cell Membrane - metabolism ; Cell Polarity ; Ependyma - cytology ; Ependyma - metabolism ; Immunoblotting ; Immunohistochemistry ; Ion Channels - metabolism ; Male ; Microscopy, Electron ; Neuroglia - metabolism ; Rats ; Rats, Wistar ; Water - metabolism ; Water-Electrolyte Balance - physiology</subject><ispartof>The Journal of neuroscience, 1997-01, Vol.17 (1), p.171-180</ispartof><rights>Copyright © 1997 Society for Neuroscience 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c544t-852f1cc5b2ba03d3428690a9884420e9e5928c592b2c8c82b4c93b8f3132cc9a3</citedby><cites>FETCH-LOGICAL-c544t-852f1cc5b2ba03d3428690a9884420e9e5928c592b2c8c82b4c93b8f3132cc9a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6793699/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6793699/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8987746$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nielsen, S</creatorcontrib><creatorcontrib>Nagelhus, E A</creatorcontrib><creatorcontrib>Amiry-Moghaddam, M</creatorcontrib><creatorcontrib>Bourque, C</creatorcontrib><creatorcontrib>Agre, P</creatorcontrib><creatorcontrib>Ottersen, O P</creatorcontrib><title>Specialized membrane domains for water transport in glial cells: high-resolution immunogold cytochemistry of aquaporin-4 in rat brain</title><title>The Journal of neuroscience</title><addtitle>J Neurosci</addtitle><description>Membrane water transport is critically involved in brain volume homeostasis and in the pathogenesis of brain edema. The cDNA encoding aquaporin-4 (AQP4) water channel protein was recently isolated from rat brain. We used immunocytochemistry and high-resolution immunogold electron microscopy to identify the cells and membrane domains that mediate water flux through AQP4. The AQP4 protein is abundant in glial cells bordering the subarachnoidal space, ventricles, and blood vessels. AQP4 is also abundant in osmosensory areas, including the supraoptic nucleus and subfornical organ. Immunogold analysis demonstrated that AQP4 is restricted to glial membranes and to subpopulations of ependymal cells. AQP4 is particularly strongly expressed in glial membranes that are in direct contact with capillaries and pia. The highly polarized AQP4 expression indicates that these cells are equipped with specific membrane domains that are specialized for water transport, thereby mediating the flow of water between glial cells and the cavities filled with CSF and the intravascular space.</description><subject>Animals</subject><subject>Aquaporin 4</subject><subject>Aquaporins</subject><subject>Astrocytes - metabolism</subject><subject>Biological Transport</subject><subject>Brain - metabolism</subject><subject>Brain - ultrastructure</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Polarity</subject><subject>Ependyma - cytology</subject><subject>Ependyma - metabolism</subject><subject>Immunoblotting</subject><subject>Immunohistochemistry</subject><subject>Ion Channels - metabolism</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Neuroglia - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Water - metabolism</subject><subject>Water-Electrolyte Balance - physiology</subject><issn>0270-6474</issn><issn>1529-2401</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1vFCEcxomxqWv1I5gQD95YeZsBejAxm1prmjax9kwYhtmlmYEtMJr13u8tazdNe4EEnuf3f3kA-EjwkjSUfb4Lbk4xW78kAmGCMCaCLIlS4hVYVIVClGPyGiwwFRi1XPA34G3OdxhjUaXH4FgqKQRvF-DhZuusN6P_63o4ualLJjjYx8n4kOEQE_xjikuw1Pe8jalAH-B6rA5o3TjmU7jx6w1KLsdxLj4G6KdpDnEdxx7aXYl24yafS9rBOEBzP5sK8QHxPSeZAmtBH96Bo8GM2b0_3Cfg9tvZr9V3dHl9frH6eolsw3lBsqEDsbbpaGcw6xmnslXYKCk5p9gp1ygqbT06aqWVtONWsU4OjDBqrTLsBHx55G7nbnK9daHONept8pNJOx2N1y9_gt_odfytW6FYq1QFfDoAUryfXS66DrdfRN1anLMWUighZFOFp49CW4PKyQ1PRQjW-xD1j6uz25_XN6sLTYTGRP8PUe9DrOYPz9t8sh5SY_8AW0afLQ</recordid><startdate>19970101</startdate><enddate>19970101</enddate><creator>Nielsen, S</creator><creator>Nagelhus, E A</creator><creator>Amiry-Moghaddam, M</creator><creator>Bourque, C</creator><creator>Agre, P</creator><creator>Ottersen, O P</creator><general>Society for Neuroscience</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970101</creationdate><title>Specialized membrane domains for water transport in glial cells: high-resolution immunogold cytochemistry of aquaporin-4 in rat brain</title><author>Nielsen, S ; Nagelhus, E A ; Amiry-Moghaddam, M ; Bourque, C ; Agre, P ; Ottersen, O P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c544t-852f1cc5b2ba03d3428690a9884420e9e5928c592b2c8c82b4c93b8f3132cc9a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Aquaporin 4</topic><topic>Aquaporins</topic><topic>Astrocytes - metabolism</topic><topic>Biological Transport</topic><topic>Brain - metabolism</topic><topic>Brain - ultrastructure</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Polarity</topic><topic>Ependyma - cytology</topic><topic>Ependyma - metabolism</topic><topic>Immunoblotting</topic><topic>Immunohistochemistry</topic><topic>Ion Channels - metabolism</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>Neuroglia - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Water - metabolism</topic><topic>Water-Electrolyte Balance - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nielsen, S</creatorcontrib><creatorcontrib>Nagelhus, E A</creatorcontrib><creatorcontrib>Amiry-Moghaddam, M</creatorcontrib><creatorcontrib>Bourque, C</creatorcontrib><creatorcontrib>Agre, P</creatorcontrib><creatorcontrib>Ottersen, O P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nielsen, S</au><au>Nagelhus, E A</au><au>Amiry-Moghaddam, M</au><au>Bourque, C</au><au>Agre, P</au><au>Ottersen, O P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specialized membrane domains for water transport in glial cells: high-resolution immunogold cytochemistry of aquaporin-4 in rat brain</atitle><jtitle>The Journal of neuroscience</jtitle><addtitle>J Neurosci</addtitle><date>1997-01-01</date><risdate>1997</risdate><volume>17</volume><issue>1</issue><spage>171</spage><epage>180</epage><pages>171-180</pages><issn>0270-6474</issn><eissn>1529-2401</eissn><abstract>Membrane water transport is critically involved in brain volume homeostasis and in the pathogenesis of brain edema. The cDNA encoding aquaporin-4 (AQP4) water channel protein was recently isolated from rat brain. We used immunocytochemistry and high-resolution immunogold electron microscopy to identify the cells and membrane domains that mediate water flux through AQP4. The AQP4 protein is abundant in glial cells bordering the subarachnoidal space, ventricles, and blood vessels. AQP4 is also abundant in osmosensory areas, including the supraoptic nucleus and subfornical organ. Immunogold analysis demonstrated that AQP4 is restricted to glial membranes and to subpopulations of ependymal cells. AQP4 is particularly strongly expressed in glial membranes that are in direct contact with capillaries and pia. The highly polarized AQP4 expression indicates that these cells are equipped with specific membrane domains that are specialized for water transport, thereby mediating the flow of water between glial cells and the cavities filled with CSF and the intravascular space.</abstract><cop>United States</cop><pub>Society for Neuroscience</pub><pmid>8987746</pmid><doi>10.1523/jneurosci.17-01-00171.1997</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Aquaporin 4 Aquaporins Astrocytes - metabolism Biological Transport Brain - metabolism Brain - ultrastructure Cell Membrane - metabolism Cell Polarity Ependyma - cytology Ependyma - metabolism Immunoblotting Immunohistochemistry Ion Channels - metabolism Male Microscopy, Electron Neuroglia - metabolism Rats Rats, Wistar Water - metabolism Water-Electrolyte Balance - physiology |
| title | Specialized membrane domains for water transport in glial cells: high-resolution immunogold cytochemistry of aquaporin-4 in rat brain |
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