A Subpopulation of Amygdala Neurons Mediates the Affective Component of Itch

Itch consists of both sensory and affective components. For chronic itch patients, the affective component of itch affects both quality of life (leading to psychological comorbidities) and disease prognosis (by promoting scratching of itchy skin). We found that acute itch stimuli, such as histamine,...

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Veröffentlicht in:The Journal of neuroscience 2019-04, Vol.39 (17), p.3345-3356
Hauptverfasser: Sanders, Kristen M, Sakai, Kent, Henry, Tyler D, Hashimoto, Takashi, Akiyama, Tasuku
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container_issue 17
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container_title The Journal of neuroscience
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creator Sanders, Kristen M
Sakai, Kent
Henry, Tyler D
Hashimoto, Takashi
Akiyama, Tasuku
description Itch consists of both sensory and affective components. For chronic itch patients, the affective component of itch affects both quality of life (leading to psychological comorbidities) and disease prognosis (by promoting scratching of itchy skin). We found that acute itch stimuli, such as histamine, induced anxiety-like behavior and increased activity (c-Fos expression) in the amygdala in adult male C57BL/6 mice. Itch stimuli also increased activity in projection areas to the amygdala, suggesting that these regions form a circuit for affective itch processing. Electrophysiological characterization of histamine-responsive amygdala neurons showed that this population was active on a behaviorally relevant timescale and partially overlapped with pain signaling. Selective optogenetic activation of histamine-responsive amygdala neurons in adult male and female Fos:CreER ;R26 mice using the Targeted Recombination in Active Populations system enhanced both scratching and anxiety-like behavior. These results highlight the importance of itch-responsive amygdala neurons in modulating itch-related affect and behavior. The sensation of itch includes an affective component that leads to stress and anxiety in chronic itch patients. We investigated the neuronal basis of affective itch in mice, with a focus on the amygdala, the key brain region for the generation of anxiety. A subpopulation of amygdala neurons responded to itch stimuli such as histamine. Optogenetic activation of histamine-responsive amygdala neurons affected both scratching and anxiety-like behavior. Therefore, this population appears to be important for mediating the affective component of itch.
doi_str_mv 10.1523/JNEUROSCI.2759-18.2019
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For chronic itch patients, the affective component of itch affects both quality of life (leading to psychological comorbidities) and disease prognosis (by promoting scratching of itchy skin). We found that acute itch stimuli, such as histamine, induced anxiety-like behavior and increased activity (c-Fos expression) in the amygdala in adult male C57BL/6 mice. Itch stimuli also increased activity in projection areas to the amygdala, suggesting that these regions form a circuit for affective itch processing. Electrophysiological characterization of histamine-responsive amygdala neurons showed that this population was active on a behaviorally relevant timescale and partially overlapped with pain signaling. Selective optogenetic activation of histamine-responsive amygdala neurons in adult male and female Fos:CreER ;R26 mice using the Targeted Recombination in Active Populations system enhanced both scratching and anxiety-like behavior. These results highlight the importance of itch-responsive amygdala neurons in modulating itch-related affect and behavior. The sensation of itch includes an affective component that leads to stress and anxiety in chronic itch patients. We investigated the neuronal basis of affective itch in mice, with a focus on the amygdala, the key brain region for the generation of anxiety. A subpopulation of amygdala neurons responded to itch stimuli such as histamine. Optogenetic activation of histamine-responsive amygdala neurons affected both scratching and anxiety-like behavior. 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subjects Amygdala
Amygdala - drug effects
Amygdala - physiopathology
Animals
Anxiety
Avoidance Learning - physiology
c-Fos protein
Chloroquine - pharmacology
Female
Histamine
Histamine - pharmacology
Male
Mice
Mice, Inbred C57BL
Neurons
Neurons - drug effects
Neurons - physiology
Optogenetics
Pain
Pruritus - physiopathology
Quality of life
Recombination
Scratching
Serotonin - pharmacology
Stimuli
title A Subpopulation of Amygdala Neurons Mediates the Affective Component of Itch
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