Biological characterization of the UW402, UW473, ONS-76 and DAOY pediatric medulloblastoma cell lines
Medulloblastoma (MB) is the most common malignant brain tumor in children. Recent advances in molecular technologies allowed to classify MB in 4 major molecular subgroups: WNT, SHH, Group 3 and Group 4. In cancer research, cancer cell lines are important for examining and manipulating molecular and...
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| Veröffentlicht in: | Cytotechnology (Dordrecht) 2019-10, Vol.71 (5), p.893-903 |
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| creator | Bonfim-Silva, Ricardo Salomão, Karina Bezerra Pimentel, Thais Valéria Costa de Andrade Menezes, Camila Cristina Branquinho de Oliveira Palma, Patrícia Vianna Bonini Fontes, Aparecida Maria |
| description | Medulloblastoma (MB) is the most common malignant brain tumor in children. Recent advances in molecular technologies allowed to classify MB in 4 major molecular subgroups: WNT, SHH, Group 3 and Group 4. In cancer research, cancer cell lines are important for examining and manipulating molecular and cellular process. However, it is important to know the characteristics of each cancer cell line prior to use, because there are some differences among them, even if they originate from the same cancer type. This study aimed to evaluate the similarities and differences among four human medulloblastoma cell lines, UW402, UW473, DAOY and ONS-76. The medulloblastoma cell lines were analyzed for (1) cell morphology, (2) immunophenotyping by flow cytometry for some specifics surface proteins, (3) expression level of adhesion molecules by RT-qPCR, (4) proliferative potential, (5) cell migration, and (6) in vivo tumorigenic potential. It was observed a relationship between cell growth and
CDH1
(E-chaderin) adhesion molecule expression and all MB cell lines showed higher levels of
CDH2
(N-chaderin) when compared to other adhesion molecule. ONS-76 showed higher gene expression of
CDH5
(VE-chaderin) and higher percentage of CD144/VE-chaderin positive cells when compared to other MB cell lines. All MB cell lines showed low percentage of CD34, CD45, CD31, CD133 positive cells and high percentage of CD44, CD105, CD106 and CD29 positive cells. The DAOY cell line showed the highest migration potential, the ONS-76 cell line showed the highest proliferative potential and only DAOY and ONS-76 cell lines showed tumorigenic potential in vivo. MB cell lines showed functional and molecular differences among them, which it should be considered by the researchers in choosing the most suitable cellular model according to the study proposal. |
| doi_str_mv | 10.1007/s10616-019-00332-3 |
| format | Article |
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CDH1
(E-chaderin) adhesion molecule expression and all MB cell lines showed higher levels of
CDH2
(N-chaderin) when compared to other adhesion molecule. ONS-76 showed higher gene expression of
CDH5
(VE-chaderin) and higher percentage of CD144/VE-chaderin positive cells when compared to other MB cell lines. All MB cell lines showed low percentage of CD34, CD45, CD31, CD133 positive cells and high percentage of CD44, CD105, CD106 and CD29 positive cells. The DAOY cell line showed the highest migration potential, the ONS-76 cell line showed the highest proliferative potential and only DAOY and ONS-76 cell lines showed tumorigenic potential in vivo. MB cell lines showed functional and molecular differences among them, which it should be considered by the researchers in choosing the most suitable cellular model according to the study proposal.</description><identifier>ISSN: 0920-9069</identifier><identifier>EISSN: 1573-0778</identifier><identifier>DOI: 10.1007/s10616-019-00332-3</identifier><identifier>PMID: 31346954</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biochemistry ; Biomedicine ; Biotechnology ; Brain tumors ; Cancer ; CD105 antigen ; CD29 antigen ; CD34 antigen ; CD44 antigen ; CD45 antigen ; Cell adhesion & migration ; Cell adhesion molecules ; Cell growth ; Cell migration ; Chemistry ; Chemistry and Materials Science ; Cytology ; E-cadherin ; Flow cytometry ; Gene expression ; Histology ; Medulloblastoma ; Microscopy ; Morphology ; Oncology ; Original ; Original Article ; Pediatrics ; Standard deviation ; Tumor cell lines ; Wnt protein</subject><ispartof>Cytotechnology (Dordrecht), 2019-10, Vol.71 (5), p.893-903</ispartof><rights>Springer Nature B.V. 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><rights>Springer Nature B.V. 2019.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c539t-55572b156ac1db87c1b17b48fc673026ceda2449d49f2f50571af3c816e0e66d3</citedby><cites>FETCH-LOGICAL-c539t-55572b156ac1db87c1b17b48fc673026ceda2449d49f2f50571af3c816e0e66d3</cites><orcidid>0000-0002-4128-0497</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787134/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918256424?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,21367,27901,27902,33721,33722,41464,42533,43781,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31346954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bonfim-Silva, Ricardo</creatorcontrib><creatorcontrib>Salomão, Karina Bezerra</creatorcontrib><creatorcontrib>Pimentel, Thais Valéria Costa de Andrade</creatorcontrib><creatorcontrib>Menezes, Camila Cristina Branquinho de Oliveira</creatorcontrib><creatorcontrib>Palma, Patrícia Vianna Bonini</creatorcontrib><creatorcontrib>Fontes, Aparecida Maria</creatorcontrib><title>Biological characterization of the UW402, UW473, ONS-76 and DAOY pediatric medulloblastoma cell lines</title><title>Cytotechnology (Dordrecht)</title><addtitle>Cytotechnology</addtitle><addtitle>Cytotechnology</addtitle><description>Medulloblastoma (MB) is the most common malignant brain tumor in children. Recent advances in molecular technologies allowed to classify MB in 4 major molecular subgroups: WNT, SHH, Group 3 and Group 4. In cancer research, cancer cell lines are important for examining and manipulating molecular and cellular process. However, it is important to know the characteristics of each cancer cell line prior to use, because there are some differences among them, even if they originate from the same cancer type. This study aimed to evaluate the similarities and differences among four human medulloblastoma cell lines, UW402, UW473, DAOY and ONS-76. The medulloblastoma cell lines were analyzed for (1) cell morphology, (2) immunophenotyping by flow cytometry for some specifics surface proteins, (3) expression level of adhesion molecules by RT-qPCR, (4) proliferative potential, (5) cell migration, and (6) in vivo tumorigenic potential. It was observed a relationship between cell growth and
CDH1
(E-chaderin) adhesion molecule expression and all MB cell lines showed higher levels of
CDH2
(N-chaderin) when compared to other adhesion molecule. ONS-76 showed higher gene expression of
CDH5
(VE-chaderin) and higher percentage of CD144/VE-chaderin positive cells when compared to other MB cell lines. All MB cell lines showed low percentage of CD34, CD45, CD31, CD133 positive cells and high percentage of CD44, CD105, CD106 and CD29 positive cells. The DAOY cell line showed the highest migration potential, the ONS-76 cell line showed the highest proliferative potential and only DAOY and ONS-76 cell lines showed tumorigenic potential in vivo. MB cell lines showed functional and molecular differences among them, which it should be considered by the researchers in choosing the most suitable cellular model according to the study proposal.</description><subject>Biochemistry</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Brain tumors</subject><subject>Cancer</subject><subject>CD105 antigen</subject><subject>CD29 antigen</subject><subject>CD34 antigen</subject><subject>CD44 antigen</subject><subject>CD45 antigen</subject><subject>Cell adhesion & migration</subject><subject>Cell adhesion molecules</subject><subject>Cell growth</subject><subject>Cell migration</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cytology</subject><subject>E-cadherin</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Histology</subject><subject>Medulloblastoma</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Oncology</subject><subject>Original</subject><subject>Original Article</subject><subject>Pediatrics</subject><subject>Standard deviation</subject><subject>Tumor cell lines</subject><subject>Wnt protein</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kTtvFDEUhS1ERJbAH6BAlmgo4nD9HjdIIbwiRWwBEaKyPB7PriPveLFnkODXM5sNgVCkusX9fHzOPQg9o3BCAfSrSkFRRYAaAsA5I_wBWlCpOQGtm4doAYYBMaDMIXpc6xUAGE35I3TIKRfKSLFA4U3MKa-idwn7tSvOj6HEX26MecC5x-M64MuvAtjxbmh-jJefPhOtsBs6_PZ0-Q1vQxfdWKLHm9BNKeU2uTrmjcM-pIRTHEJ9gg56l2p4ejOP0OX7d1_OPpKL5Yfzs9ML4iU3I5FSatZSqZynXdtoT1uqW9H0XmkOTPnQOSaE6YTpWS9Baup67huqAgSlOn6EXu91t1M7u_FhGItLdlvixpWfNrto726GuLar_MMq3cyXEbPAyxuBkr9PoY52E-suhxtCnqplTEktjGj4jL74D73KUxnmeJYZ2jCpBBP3Uhx4Q4FdU2xP-ZJrLaG_tUzB7rq2-67t3LW97truDDz_N-ztkz_lzgDfA3VeDatQ_v59j-xvlYWxvg</recordid><startdate>20191001</startdate><enddate>20191001</enddate><creator>Bonfim-Silva, Ricardo</creator><creator>Salomão, Karina Bezerra</creator><creator>Pimentel, Thais Valéria Costa de Andrade</creator><creator>Menezes, Camila Cristina Branquinho de Oliveira</creator><creator>Palma, Patrícia Vianna Bonini</creator><creator>Fontes, Aparecida Maria</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4128-0497</orcidid></search><sort><creationdate>20191001</creationdate><title>Biological characterization of the UW402, UW473, ONS-76 and DAOY pediatric medulloblastoma cell lines</title><author>Bonfim-Silva, Ricardo ; Salomão, Karina Bezerra ; Pimentel, Thais Valéria Costa de Andrade ; Menezes, Camila Cristina Branquinho de Oliveira ; Palma, Patrícia Vianna Bonini ; Fontes, Aparecida Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c539t-55572b156ac1db87c1b17b48fc673026ceda2449d49f2f50571af3c816e0e66d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Biochemistry</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Brain tumors</topic><topic>Cancer</topic><topic>CD105 antigen</topic><topic>CD29 antigen</topic><topic>CD34 antigen</topic><topic>CD44 antigen</topic><topic>CD45 antigen</topic><topic>Cell adhesion & migration</topic><topic>Cell adhesion molecules</topic><topic>Cell growth</topic><topic>Cell migration</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cytology</topic><topic>E-cadherin</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Histology</topic><topic>Medulloblastoma</topic><topic>Microscopy</topic><topic>Morphology</topic><topic>Oncology</topic><topic>Original</topic><topic>Original Article</topic><topic>Pediatrics</topic><topic>Standard deviation</topic><topic>Tumor cell lines</topic><topic>Wnt protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bonfim-Silva, Ricardo</creatorcontrib><creatorcontrib>Salomão, Karina Bezerra</creatorcontrib><creatorcontrib>Pimentel, Thais Valéria Costa de Andrade</creatorcontrib><creatorcontrib>Menezes, Camila Cristina Branquinho de Oliveira</creatorcontrib><creatorcontrib>Palma, Patrícia Vianna Bonini</creatorcontrib><creatorcontrib>Fontes, Aparecida Maria</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytotechnology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bonfim-Silva, Ricardo</au><au>Salomão, Karina Bezerra</au><au>Pimentel, Thais Valéria Costa de Andrade</au><au>Menezes, Camila Cristina Branquinho de Oliveira</au><au>Palma, Patrícia Vianna Bonini</au><au>Fontes, Aparecida Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biological characterization of the UW402, UW473, ONS-76 and DAOY pediatric medulloblastoma cell lines</atitle><jtitle>Cytotechnology (Dordrecht)</jtitle><stitle>Cytotechnology</stitle><addtitle>Cytotechnology</addtitle><date>2019-10-01</date><risdate>2019</risdate><volume>71</volume><issue>5</issue><spage>893</spage><epage>903</epage><pages>893-903</pages><issn>0920-9069</issn><eissn>1573-0778</eissn><abstract>Medulloblastoma (MB) is the most common malignant brain tumor in children. Recent advances in molecular technologies allowed to classify MB in 4 major molecular subgroups: WNT, SHH, Group 3 and Group 4. In cancer research, cancer cell lines are important for examining and manipulating molecular and cellular process. However, it is important to know the characteristics of each cancer cell line prior to use, because there are some differences among them, even if they originate from the same cancer type. This study aimed to evaluate the similarities and differences among four human medulloblastoma cell lines, UW402, UW473, DAOY and ONS-76. The medulloblastoma cell lines were analyzed for (1) cell morphology, (2) immunophenotyping by flow cytometry for some specifics surface proteins, (3) expression level of adhesion molecules by RT-qPCR, (4) proliferative potential, (5) cell migration, and (6) in vivo tumorigenic potential. It was observed a relationship between cell growth and
CDH1
(E-chaderin) adhesion molecule expression and all MB cell lines showed higher levels of
CDH2
(N-chaderin) when compared to other adhesion molecule. ONS-76 showed higher gene expression of
CDH5
(VE-chaderin) and higher percentage of CD144/VE-chaderin positive cells when compared to other MB cell lines. All MB cell lines showed low percentage of CD34, CD45, CD31, CD133 positive cells and high percentage of CD44, CD105, CD106 and CD29 positive cells. The DAOY cell line showed the highest migration potential, the ONS-76 cell line showed the highest proliferative potential and only DAOY and ONS-76 cell lines showed tumorigenic potential in vivo. MB cell lines showed functional and molecular differences among them, which it should be considered by the researchers in choosing the most suitable cellular model according to the study proposal.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>31346954</pmid><doi>10.1007/s10616-019-00332-3</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4128-0497</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Biochemistry Biomedicine Biotechnology Brain tumors Cancer CD105 antigen CD29 antigen CD34 antigen CD44 antigen CD45 antigen Cell adhesion & migration Cell adhesion molecules Cell growth Cell migration Chemistry Chemistry and Materials Science Cytology E-cadherin Flow cytometry Gene expression Histology Medulloblastoma Microscopy Morphology Oncology Original Original Article Pediatrics Standard deviation Tumor cell lines Wnt protein |
| title | Biological characterization of the UW402, UW473, ONS-76 and DAOY pediatric medulloblastoma cell lines |
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