Actinobacillus species isolated from Japanese Thoroughbred racehorses in the last two decades
Actinobacillus species are known to be pathogenic to horses. To clarify etiological agents of actinobacillosis in Japanese adult horses, 27 isolates from Japanese Thoroughbred racehorses putatively identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry as Actinoba...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2019, Vol.81(9), pp.1234-1237 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | UCHIDA-FUJII, Eri NIWA, Hidekazu KINOSHITA, Yuta NUKADA, Toshio |
| description | Actinobacillus species are known to be pathogenic to horses. To clarify etiological agents of actinobacillosis in Japanese adult horses, 27 isolates from Japanese Thoroughbred racehorses putatively identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry as Actinobacillus were further identified by PCR of the A. equuli toxin gene, by CAMP test, and by 16S rRNA sequencing analysis. Actinobacillus equuli subsp. haemolyticus was isolated most frequently (16/27) and was related to respiratory infections. Actinobacillus equuli subsp. equuli (4/27) was isolated from chronic cases or concomitant with other bacterial infections. The remainder were A. pleuropneumoniae, unclassified Actinobacillus species and Pasteurella caballi. Actinobacillus equuli including subsp. haemolyticus and subsp. equuli were the species most frequently isolated from equine actinobacillosis in Japan. |
| doi_str_mv | 10.1292/jvms.19-0192 |
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To clarify etiological agents of actinobacillosis in Japanese adult horses, 27 isolates from Japanese Thoroughbred racehorses putatively identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry as Actinobacillus were further identified by PCR of the A. equuli toxin gene, by CAMP test, and by 16S rRNA sequencing analysis. Actinobacillus equuli subsp. haemolyticus was isolated most frequently (16/27) and was related to respiratory infections. Actinobacillus equuli subsp. equuli (4/27) was isolated from chronic cases or concomitant with other bacterial infections. The remainder were A. pleuropneumoniae, unclassified Actinobacillus species and Pasteurella caballi. Actinobacillus equuli including subsp. haemolyticus and subsp. equuli were the species most frequently isolated from equine actinobacillosis in Japan.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.19-0192</identifier><identifier>PMID: 31292334</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>16S rRNA ; Actinobacillosis ; Actinobacillus ; Actinobacillus - classification ; Actinobacillus - genetics ; Actinobacillus - isolation & purification ; Actinobacillus Infections - veterinary ; Animals ; Bacterial Proteins - genetics ; Bacteriology ; Camp test ; DNA, Bacterial - analysis ; Horse Diseases - etiology ; Horse Diseases - microbiology ; Horses ; Infections ; Ionization ; Ions ; Japan ; Mass spectrometry ; Mass spectroscopy ; matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) ; Pasteurella - isolation & purification ; Polymerase Chain Reaction - veterinary ; Racehorses ; repeats-in-toxin (RTX) ; RNA, Ribosomal, 16S ; rRNA 16S ; Species ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary ; Thoroughbred</subject><ispartof>Journal of Veterinary Medical Science, 2019, Vol.81(9), pp.1234-1237</ispartof><rights>2019 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency Sep 2019</rights><rights>2019 The Japanese Society of Veterinary Science 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c662t-e3c52e7dc588a2b943cbb162239b66b8ec59aa1765b8ffafe870a21fcb91daf33</citedby><cites>FETCH-LOGICAL-c662t-e3c52e7dc588a2b943cbb162239b66b8ec59aa1765b8ffafe870a21fcb91daf33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6785623/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6785623/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1876,4009,27902,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31292334$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>UCHIDA-FUJII, Eri</creatorcontrib><creatorcontrib>NIWA, Hidekazu</creatorcontrib><creatorcontrib>KINOSHITA, Yuta</creatorcontrib><creatorcontrib>NUKADA, Toshio</creatorcontrib><title>Actinobacillus species isolated from Japanese Thoroughbred racehorses in the last two decades</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Actinobacillus species are known to be pathogenic to horses. To clarify etiological agents of actinobacillosis in Japanese adult horses, 27 isolates from Japanese Thoroughbred racehorses putatively identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry as Actinobacillus were further identified by PCR of the A. equuli toxin gene, by CAMP test, and by 16S rRNA sequencing analysis. Actinobacillus equuli subsp. haemolyticus was isolated most frequently (16/27) and was related to respiratory infections. Actinobacillus equuli subsp. equuli (4/27) was isolated from chronic cases or concomitant with other bacterial infections. The remainder were A. pleuropneumoniae, unclassified Actinobacillus species and Pasteurella caballi. Actinobacillus equuli including subsp. haemolyticus and subsp. equuli were the species most frequently isolated from equine actinobacillosis in Japan.</description><subject>16S rRNA</subject><subject>Actinobacillosis</subject><subject>Actinobacillus</subject><subject>Actinobacillus - classification</subject><subject>Actinobacillus - genetics</subject><subject>Actinobacillus - isolation & purification</subject><subject>Actinobacillus Infections - veterinary</subject><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Camp test</subject><subject>DNA, Bacterial - analysis</subject><subject>Horse Diseases - etiology</subject><subject>Horse Diseases - microbiology</subject><subject>Horses</subject><subject>Infections</subject><subject>Ionization</subject><subject>Ions</subject><subject>Japan</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS)</subject><subject>Pasteurella - isolation & purification</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Racehorses</subject><subject>repeats-in-toxin (RTX)</subject><subject>RNA, Ribosomal, 16S</subject><subject>rRNA 16S</subject><subject>Species</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary</subject><subject>Thoroughbred</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1v1DAQxS1ERZfCjTOKxIUDKf5InPiCVCqgRZW4lCOyxs5k45UTL7ZTxH9Pol1WlItH1vvNk58fIa8YvWRc8fe7hzFdMlVSpvgTsmGiasqmEuop2VDFZNnwmp6T5yntKOWskuoZORfrphDVhvy4stlNwYB13s-pSHu0DlPhUvCQsSv6GMbiK-xhwoTF_RBimLeDiYsUweJyTys-FXnAwkPKRf4Vig4tdJhekLMefMKXx3lBvn_-dH99U959-3J7fXVXWil5LlHYmmPT2bptgRtVCWsMk5wLZaQ0LdpaAbBG1qbte-ixbShw1lujWAe9EBfkw8F3P5sRO4tTjuD1ProR4m8dwOnHyuQGvQ0PWjZtLflq8PZoEMPPGVPWo0sWvV9ihzlpzmvJqGxrvqBv_kN3YY7TEm-hFFWUNYwt1LsDZWNIKWJ_egyjev19vfammdJrbwv--t8AJ_hvUQvw8QDsUoYtngCI2VmPB7eWabUeR9eTaAeIGifxB650ro0</recordid><startdate>2019</startdate><enddate>2019</enddate><creator>UCHIDA-FUJII, Eri</creator><creator>NIWA, Hidekazu</creator><creator>KINOSHITA, Yuta</creator><creator>NUKADA, Toshio</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2019</creationdate><title>Actinobacillus species isolated from Japanese Thoroughbred racehorses in the last two decades</title><author>UCHIDA-FUJII, Eri ; NIWA, Hidekazu ; KINOSHITA, Yuta ; NUKADA, Toshio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c662t-e3c52e7dc588a2b943cbb162239b66b8ec59aa1765b8ffafe870a21fcb91daf33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>16S rRNA</topic><topic>Actinobacillosis</topic><topic>Actinobacillus</topic><topic>Actinobacillus - classification</topic><topic>Actinobacillus - genetics</topic><topic>Actinobacillus - isolation & purification</topic><topic>Actinobacillus Infections - veterinary</topic><topic>Animals</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Camp test</topic><topic>DNA, Bacterial - analysis</topic><topic>Horse Diseases - etiology</topic><topic>Horse Diseases - microbiology</topic><topic>Horses</topic><topic>Infections</topic><topic>Ionization</topic><topic>Ions</topic><topic>Japan</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS)</topic><topic>Pasteurella - isolation & purification</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Racehorses</topic><topic>repeats-in-toxin (RTX)</topic><topic>RNA, Ribosomal, 16S</topic><topic>rRNA 16S</topic><topic>Species</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary</topic><topic>Thoroughbred</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>UCHIDA-FUJII, Eri</creatorcontrib><creatorcontrib>NIWA, Hidekazu</creatorcontrib><creatorcontrib>KINOSHITA, Yuta</creatorcontrib><creatorcontrib>NUKADA, Toshio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>UCHIDA-FUJII, Eri</au><au>NIWA, Hidekazu</au><au>KINOSHITA, Yuta</au><au>NUKADA, Toshio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Actinobacillus species isolated from Japanese Thoroughbred racehorses in the last two decades</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2019</date><risdate>2019</risdate><volume>81</volume><issue>9</issue><spage>1234</spage><epage>1237</epage><pages>1234-1237</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Actinobacillus species are known to be pathogenic to horses. To clarify etiological agents of actinobacillosis in Japanese adult horses, 27 isolates from Japanese Thoroughbred racehorses putatively identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry as Actinobacillus were further identified by PCR of the A. equuli toxin gene, by CAMP test, and by 16S rRNA sequencing analysis. Actinobacillus equuli subsp. haemolyticus was isolated most frequently (16/27) and was related to respiratory infections. Actinobacillus equuli subsp. equuli (4/27) was isolated from chronic cases or concomitant with other bacterial infections. The remainder were A. pleuropneumoniae, unclassified Actinobacillus species and Pasteurella caballi. Actinobacillus equuli including subsp. haemolyticus and subsp. equuli were the species most frequently isolated from equine actinobacillosis in Japan.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>31292334</pmid><doi>10.1292/jvms.19-0192</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Veterinary Medical Science, 2019, Vol.81(9), pp.1234-1237 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; PubMed Central |
| subjects | 16S rRNA Actinobacillosis Actinobacillus Actinobacillus - classification Actinobacillus - genetics Actinobacillus - isolation & purification Actinobacillus Infections - veterinary Animals Bacterial Proteins - genetics Bacteriology Camp test DNA, Bacterial - analysis Horse Diseases - etiology Horse Diseases - microbiology Horses Infections Ionization Ions Japan Mass spectrometry Mass spectroscopy matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) Pasteurella - isolation & purification Polymerase Chain Reaction - veterinary Racehorses repeats-in-toxin (RTX) RNA, Ribosomal, 16S rRNA 16S Species Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary Thoroughbred |
| title | Actinobacillus species isolated from Japanese Thoroughbred racehorses in the last two decades |
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