Embryonic Neurons Adapt to the Inhibitory Proteoglycan Aggrecan by Increasing Integrin Expression
The primary mediators of cell migration during development, wound healing and metastasis, are receptors of the integrin family. In the developing and regenerating nervous system, chondroitin sulfate proteoglycans (CSPGs) inhibit the integrin-dependent migration of neuronal growth cones. Here we repo...
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| Veröffentlicht in: | The Journal of neuroscience 1999-11, Vol.19 (22), p.10036-10043 |
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| creator | Condic, Maureen L Snow, Diane M Letourneau, Paul C |
| description | The primary mediators of cell migration during development, wound healing and metastasis, are receptors of the integrin family. In the developing and regenerating nervous system, chondroitin sulfate proteoglycans (CSPGs) inhibit the integrin-dependent migration of neuronal growth cones. Here we report that embryonic sensory neurons cultured on the growth-promoting molecule laminin in combination with the inhibitory CSPG aggrecan rapidly adapt to inhibition. Adaptation is associated with a two- to threefold increase in the levels of RNA and surface protein for two laminin receptors, integrin alpha6beta1 and alpha3beta1, indicating that integrin expression is regulated by aggrecan. Increased integrin expression is associated both with increases in neuronal cell adhesion/outgrowth and with decreases in the ability of aggrecan to inhibit cell adhesion. Directly increasing integrin expression by adenoviral infection is sufficient to eliminate the inhibitory effects of aggrecan, indicating that upregulation of integrin receptors may promote neuronal regeneration in the presence of inhibitory matrix components. |
| doi_str_mv | 10.1523/jneurosci.19-22-10036.1999 |
| format | Article |
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In the developing and regenerating nervous system, chondroitin sulfate proteoglycans (CSPGs) inhibit the integrin-dependent migration of neuronal growth cones. Here we report that embryonic sensory neurons cultured on the growth-promoting molecule laminin in combination with the inhibitory CSPG aggrecan rapidly adapt to inhibition. Adaptation is associated with a two- to threefold increase in the levels of RNA and surface protein for two laminin receptors, integrin alpha6beta1 and alpha3beta1, indicating that integrin expression is regulated by aggrecan. Increased integrin expression is associated both with increases in neuronal cell adhesion/outgrowth and with decreases in the ability of aggrecan to inhibit cell adhesion. Directly increasing integrin expression by adenoviral infection is sufficient to eliminate the inhibitory effects of aggrecan, indicating that upregulation of integrin receptors may promote neuronal regeneration in the presence of inhibitory matrix components.</description><identifier>ISSN: 0270-6474</identifier><identifier>EISSN: 1529-2401</identifier><identifier>DOI: 10.1523/jneurosci.19-22-10036.1999</identifier><identifier>PMID: 10559411</identifier><language>eng</language><publisher>United States: Soc Neuroscience</publisher><subject>aggrecan ; Aggrecans ; Animals ; Cells, Cultured ; Chick Embryo ; Chondroitin Sulfate Proteoglycans - pharmacology ; Extracellular Matrix Proteins ; Ganglia, Spinal - cytology ; Ganglia, Spinal - embryology ; Gene Expression Regulation - drug effects ; Gene Expression Regulation - physiology ; Integrin alpha3beta1 ; Integrin alpha6beta1 ; Integrins - genetics ; Lectins, C-Type ; Neurites - physiology ; Neurons - cytology ; Neurons - drug effects ; Neurons - physiology ; Protein Biosynthesis ; Proteoglycans - pharmacology ; Transcription, Genetic</subject><ispartof>The Journal of neuroscience, 1999-11, Vol.19 (22), p.10036-10043</ispartof><rights>Copyright © 1999 Society for Neuroscience 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-8be8cfbee2581badaf7eb1ca64d3b2ee43be83a0b4304f0666cb22adaf3832033</citedby><cites>FETCH-LOGICAL-c521t-8be8cfbee2581badaf7eb1ca64d3b2ee43be83a0b4304f0666cb22adaf3832033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6782993/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6782993/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,27907,27908,53774,53776</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10559411$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Condic, Maureen L</creatorcontrib><creatorcontrib>Snow, Diane M</creatorcontrib><creatorcontrib>Letourneau, Paul C</creatorcontrib><title>Embryonic Neurons Adapt to the Inhibitory Proteoglycan Aggrecan by Increasing Integrin Expression</title><title>The Journal of neuroscience</title><addtitle>J Neurosci</addtitle><description>The primary mediators of cell migration during development, wound healing and metastasis, are receptors of the integrin family. In the developing and regenerating nervous system, chondroitin sulfate proteoglycans (CSPGs) inhibit the integrin-dependent migration of neuronal growth cones. Here we report that embryonic sensory neurons cultured on the growth-promoting molecule laminin in combination with the inhibitory CSPG aggrecan rapidly adapt to inhibition. Adaptation is associated with a two- to threefold increase in the levels of RNA and surface protein for two laminin receptors, integrin alpha6beta1 and alpha3beta1, indicating that integrin expression is regulated by aggrecan. Increased integrin expression is associated both with increases in neuronal cell adhesion/outgrowth and with decreases in the ability of aggrecan to inhibit cell adhesion. Directly increasing integrin expression by adenoviral infection is sufficient to eliminate the inhibitory effects of aggrecan, indicating that upregulation of integrin receptors may promote neuronal regeneration in the presence of inhibitory matrix components.</description><subject>aggrecan</subject><subject>Aggrecans</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Chick Embryo</subject><subject>Chondroitin Sulfate Proteoglycans - pharmacology</subject><subject>Extracellular Matrix Proteins</subject><subject>Ganglia, Spinal - cytology</subject><subject>Ganglia, Spinal - embryology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene Expression Regulation - physiology</subject><subject>Integrin alpha3beta1</subject><subject>Integrin alpha6beta1</subject><subject>Integrins - genetics</subject><subject>Lectins, C-Type</subject><subject>Neurites - physiology</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>Protein Biosynthesis</subject><subject>Proteoglycans - pharmacology</subject><subject>Transcription, Genetic</subject><issn>0270-6474</issn><issn>1529-2401</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkV1v0zAUhi0EYmXjL6CIC8RNhr_yxQVSVRXoNG0TsGvLdk8ST4nd2S4l_x6HTlO58iv7Oc-x9CL0nuBLUlD26cHC3rugzSVpckpzgjErU26aF2iRiHTJMXmJFphWOC95xc_QmxAeMMYVJtVrdEZwUTSckAWS61H5yVmjs5vZakO23MpdzKLLYg_ZxvZGmej8lN15F8F1w6SlzZZd52EOakqM9iCDsV2KETpvbLb-s_MQgnH2Ar1q5RDg7dN5ju6_rn-tvufXt982q-V1rgtKYl4rqHWrAGhREyW3sq1AES1LvmWKAnCWACax4gzzFpdlqRWlM8dqRjFj5-jL0bvbqxG2Gmz0chA7b0bpJ-GkEf-_WNOLzv0WZVXTppkFH54E3j3uIUQxmqBhGKQFtw-CVBzXvMIJ_HwEdWoheGiflxAs5obE1c36_sftz9VGkEZQKv41JOaG0vC702-ejB4rScDHI9Cbrj8YDyKMchgSTsThcDgRsr9zb6Cm</recordid><startdate>19991115</startdate><enddate>19991115</enddate><creator>Condic, Maureen L</creator><creator>Snow, Diane M</creator><creator>Letourneau, Paul C</creator><general>Soc Neuroscience</general><general>Society for Neuroscience</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>5PM</scope></search><sort><creationdate>19991115</creationdate><title>Embryonic Neurons Adapt to the Inhibitory Proteoglycan Aggrecan by Increasing Integrin Expression</title><author>Condic, Maureen L ; Snow, Diane M ; Letourneau, Paul C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-8be8cfbee2581badaf7eb1ca64d3b2ee43be83a0b4304f0666cb22adaf3832033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>aggrecan</topic><topic>Aggrecans</topic><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Chick Embryo</topic><topic>Chondroitin Sulfate Proteoglycans - pharmacology</topic><topic>Extracellular Matrix Proteins</topic><topic>Ganglia, Spinal - cytology</topic><topic>Ganglia, Spinal - embryology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Gene Expression Regulation - physiology</topic><topic>Integrin alpha3beta1</topic><topic>Integrin alpha6beta1</topic><topic>Integrins - genetics</topic><topic>Lectins, C-Type</topic><topic>Neurites - physiology</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Neurons - physiology</topic><topic>Protein Biosynthesis</topic><topic>Proteoglycans - pharmacology</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Condic, Maureen L</creatorcontrib><creatorcontrib>Snow, Diane M</creatorcontrib><creatorcontrib>Letourneau, Paul C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Condic, Maureen L</au><au>Snow, Diane M</au><au>Letourneau, Paul C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Embryonic Neurons Adapt to the Inhibitory Proteoglycan Aggrecan by Increasing Integrin Expression</atitle><jtitle>The Journal of neuroscience</jtitle><addtitle>J Neurosci</addtitle><date>1999-11-15</date><risdate>1999</risdate><volume>19</volume><issue>22</issue><spage>10036</spage><epage>10043</epage><pages>10036-10043</pages><issn>0270-6474</issn><eissn>1529-2401</eissn><abstract>The primary mediators of cell migration during development, wound healing and metastasis, are receptors of the integrin family. In the developing and regenerating nervous system, chondroitin sulfate proteoglycans (CSPGs) inhibit the integrin-dependent migration of neuronal growth cones. Here we report that embryonic sensory neurons cultured on the growth-promoting molecule laminin in combination with the inhibitory CSPG aggrecan rapidly adapt to inhibition. Adaptation is associated with a two- to threefold increase in the levels of RNA and surface protein for two laminin receptors, integrin alpha6beta1 and alpha3beta1, indicating that integrin expression is regulated by aggrecan. Increased integrin expression is associated both with increases in neuronal cell adhesion/outgrowth and with decreases in the ability of aggrecan to inhibit cell adhesion. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | aggrecan Aggrecans Animals Cells, Cultured Chick Embryo Chondroitin Sulfate Proteoglycans - pharmacology Extracellular Matrix Proteins Ganglia, Spinal - cytology Ganglia, Spinal - embryology Gene Expression Regulation - drug effects Gene Expression Regulation - physiology Integrin alpha3beta1 Integrin alpha6beta1 Integrins - genetics Lectins, C-Type Neurites - physiology Neurons - cytology Neurons - drug effects Neurons - physiology Protein Biosynthesis Proteoglycans - pharmacology Transcription, Genetic |
| title | Embryonic Neurons Adapt to the Inhibitory Proteoglycan Aggrecan by Increasing Integrin Expression |
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