Surface Pre-Reacted Glass Filler Contributes to Tertiary Dentin Formation through a Mechanism Different Than That of Hydraulic Calcium-Silicate Cement
The induction of tissue mineralization and the mechanism by which surface pre-reacted glass-ionomer (S-PRG) cement influences pulpal healing remain unclear. We evaluated S-PRG cement-induced tertiary dentin formation in vivo, and its effect on the pulp cell healing process in vitro. Induced tertiary...
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| Veröffentlicht in: | Journal of clinical medicine 2019-09, Vol.8 (9), p.1440 |
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| creator | Okamoto, Motoki Ali, Manahil Komichi, Shungo Watanabe, Masakatsu Huang, Hailing Ito, Yuki Miura, Jiro Hirose, Yujiro Mizuhira, Manabu Takahashi, Yusuke Okuzaki, Daisuke Kawabata, Shigetada Imazato, Satoshi Hayashi, Mikako |
| description | The induction of tissue mineralization and the mechanism by which surface pre-reacted glass-ionomer (S-PRG) cement influences pulpal healing remain unclear. We evaluated S-PRG cement-induced tertiary dentin formation in vivo, and its effect on the pulp cell healing process in vitro. Induced tertiary dentin formation was evaluated with micro-computed tomography (μCT) and scanning electron microscopy (SEM). The distribution of elements from the S-PRG cement in pulpal tissue was confirmed by micro-X-ray fluorescence (μXRF). The effects of S-PRG cement on cytotoxicity, proliferation, formation of mineralized nodules, and gene expression in human dental pulp stem cells (hDPSCs) were assessed in vitro. μCT and SEM revealed that S-PRG induced tertiary dentin formation with similar characteristics to that induced by hydraulic calcium-silicate cement (ProRoot mineral trioxide aggregate (MTA)). μXRF showed Sr and Si ion transfer into pulpal tissue from S-PRG cement. Notably, S-PRG cement and MTA showed similar biocompatibility. A co-culture of hDPSCs and S-PRG discs promoted mineralized nodule formation on surrounding cells. Additionally, S-PRG cement regulated the expression of genes related to osteo/dentinogenic differentiation. MTA and S-PRG regulated gene expression in hDPSCs, but the patterns of regulation differed. S-PRG cement upregulated
and
gene expression. These findings showed that S-PRG and MTA exhibit similar effects on dental pulp through different mechanisms. |
| doi_str_mv | 10.3390/jcm8091440 |
| format | Article |
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and
gene expression. These findings showed that S-PRG and MTA exhibit similar effects on dental pulp through different mechanisms.</description><identifier>ISSN: 2077-0383</identifier><identifier>EISSN: 2077-0383</identifier><identifier>DOI: 10.3390/jcm8091440</identifier><identifier>PMID: 31514356</identifier><language>eng</language><publisher>Switzerland: MDPI</publisher><ispartof>Journal of clinical medicine, 2019-09, Vol.8 (9), p.1440</ispartof><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-92a47a4ba6219782f5f3e45bc2dd0e7a006199d5ab46a17e13cab9c5484bab333</citedby><cites>FETCH-LOGICAL-c378t-92a47a4ba6219782f5f3e45bc2dd0e7a006199d5ab46a17e13cab9c5484bab333</cites><orcidid>0000-0002-0000-025X ; 0000-0001-6338-4767 ; 0000-0002-3759-5031 ; 0000-0002-4552-783X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780685/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780685/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31514356$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okamoto, Motoki</creatorcontrib><creatorcontrib>Ali, Manahil</creatorcontrib><creatorcontrib>Komichi, Shungo</creatorcontrib><creatorcontrib>Watanabe, Masakatsu</creatorcontrib><creatorcontrib>Huang, Hailing</creatorcontrib><creatorcontrib>Ito, Yuki</creatorcontrib><creatorcontrib>Miura, Jiro</creatorcontrib><creatorcontrib>Hirose, Yujiro</creatorcontrib><creatorcontrib>Mizuhira, Manabu</creatorcontrib><creatorcontrib>Takahashi, Yusuke</creatorcontrib><creatorcontrib>Okuzaki, Daisuke</creatorcontrib><creatorcontrib>Kawabata, Shigetada</creatorcontrib><creatorcontrib>Imazato, Satoshi</creatorcontrib><creatorcontrib>Hayashi, Mikako</creatorcontrib><title>Surface Pre-Reacted Glass Filler Contributes to Tertiary Dentin Formation through a Mechanism Different Than That of Hydraulic Calcium-Silicate Cement</title><title>Journal of clinical medicine</title><addtitle>J Clin Med</addtitle><description>The induction of tissue mineralization and the mechanism by which surface pre-reacted glass-ionomer (S-PRG) cement influences pulpal healing remain unclear. We evaluated S-PRG cement-induced tertiary dentin formation in vivo, and its effect on the pulp cell healing process in vitro. Induced tertiary dentin formation was evaluated with micro-computed tomography (μCT) and scanning electron microscopy (SEM). The distribution of elements from the S-PRG cement in pulpal tissue was confirmed by micro-X-ray fluorescence (μXRF). The effects of S-PRG cement on cytotoxicity, proliferation, formation of mineralized nodules, and gene expression in human dental pulp stem cells (hDPSCs) were assessed in vitro. μCT and SEM revealed that S-PRG induced tertiary dentin formation with similar characteristics to that induced by hydraulic calcium-silicate cement (ProRoot mineral trioxide aggregate (MTA)). μXRF showed Sr and Si ion transfer into pulpal tissue from S-PRG cement. Notably, S-PRG cement and MTA showed similar biocompatibility. A co-culture of hDPSCs and S-PRG discs promoted mineralized nodule formation on surrounding cells. Additionally, S-PRG cement regulated the expression of genes related to osteo/dentinogenic differentiation. MTA and S-PRG regulated gene expression in hDPSCs, but the patterns of regulation differed. S-PRG cement upregulated
and
gene expression. These findings showed that S-PRG and MTA exhibit similar effects on dental pulp through different mechanisms.</description><issn>2077-0383</issn><issn>2077-0383</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpVkdFOHSEQhjdNTTXWmz5Aw2XTZFtY2AVumjRrjzax0ejpNZllZz0YdrHAmvgifd5itNbOBQMzH_9A_qp6x-gnzjX9fGNnRTUTgr6qDhoqZU254q9f7Pero5RuaAmlRMPkm2qfs5YJ3nYH1e-rNU5gkVxErC8RbMaRnHhIiWyc9xhJH5Yc3bBmTCQHssWYHcR7coxLdgvZhDhDdmEheRfDer0jQH6g3cHi0kyO3TRhLCTZlsrDkkmYyOn9GGH1zpIevHXrXF-5coKMpMe54G-rvQl8wqOnfFj93Hzb9qf12fnJ9_7rWW25VLnWDQgJYoCuYVqqZmonjqIdbDOOFCVQ2jGtxxYG0QGTyLiFQdtWqHJn4JwfVl8edW_XYcbRltERvLmNbi5_NAGc-b-zuJ25Dnemk4p2qi0CH54EYvi1Yspmdsmi97BgWJNpGk01pVKLgn58RG0MKUWcnscwah68NP-8LPD7lw97Rv86x_8Ao5Cc8A</recordid><startdate>20190911</startdate><enddate>20190911</enddate><creator>Okamoto, Motoki</creator><creator>Ali, Manahil</creator><creator>Komichi, Shungo</creator><creator>Watanabe, Masakatsu</creator><creator>Huang, Hailing</creator><creator>Ito, Yuki</creator><creator>Miura, Jiro</creator><creator>Hirose, Yujiro</creator><creator>Mizuhira, Manabu</creator><creator>Takahashi, Yusuke</creator><creator>Okuzaki, Daisuke</creator><creator>Kawabata, Shigetada</creator><creator>Imazato, Satoshi</creator><creator>Hayashi, Mikako</creator><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0000-025X</orcidid><orcidid>https://orcid.org/0000-0001-6338-4767</orcidid><orcidid>https://orcid.org/0000-0002-3759-5031</orcidid><orcidid>https://orcid.org/0000-0002-4552-783X</orcidid></search><sort><creationdate>20190911</creationdate><title>Surface Pre-Reacted Glass Filler Contributes to Tertiary Dentin Formation through a Mechanism Different Than That of Hydraulic Calcium-Silicate Cement</title><author>Okamoto, Motoki ; Ali, Manahil ; Komichi, Shungo ; Watanabe, Masakatsu ; Huang, Hailing ; Ito, Yuki ; Miura, Jiro ; Hirose, Yujiro ; Mizuhira, Manabu ; Takahashi, Yusuke ; Okuzaki, Daisuke ; Kawabata, Shigetada ; Imazato, Satoshi ; Hayashi, Mikako</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-92a47a4ba6219782f5f3e45bc2dd0e7a006199d5ab46a17e13cab9c5484bab333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okamoto, Motoki</creatorcontrib><creatorcontrib>Ali, Manahil</creatorcontrib><creatorcontrib>Komichi, Shungo</creatorcontrib><creatorcontrib>Watanabe, Masakatsu</creatorcontrib><creatorcontrib>Huang, Hailing</creatorcontrib><creatorcontrib>Ito, Yuki</creatorcontrib><creatorcontrib>Miura, Jiro</creatorcontrib><creatorcontrib>Hirose, Yujiro</creatorcontrib><creatorcontrib>Mizuhira, Manabu</creatorcontrib><creatorcontrib>Takahashi, Yusuke</creatorcontrib><creatorcontrib>Okuzaki, Daisuke</creatorcontrib><creatorcontrib>Kawabata, Shigetada</creatorcontrib><creatorcontrib>Imazato, Satoshi</creatorcontrib><creatorcontrib>Hayashi, Mikako</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okamoto, Motoki</au><au>Ali, Manahil</au><au>Komichi, Shungo</au><au>Watanabe, Masakatsu</au><au>Huang, Hailing</au><au>Ito, Yuki</au><au>Miura, Jiro</au><au>Hirose, Yujiro</au><au>Mizuhira, Manabu</au><au>Takahashi, Yusuke</au><au>Okuzaki, Daisuke</au><au>Kawabata, Shigetada</au><au>Imazato, Satoshi</au><au>Hayashi, Mikako</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surface Pre-Reacted Glass Filler Contributes to Tertiary Dentin Formation through a Mechanism Different Than That of Hydraulic Calcium-Silicate Cement</atitle><jtitle>Journal of clinical medicine</jtitle><addtitle>J Clin Med</addtitle><date>2019-09-11</date><risdate>2019</risdate><volume>8</volume><issue>9</issue><spage>1440</spage><pages>1440-</pages><issn>2077-0383</issn><eissn>2077-0383</eissn><abstract>The induction of tissue mineralization and the mechanism by which surface pre-reacted glass-ionomer (S-PRG) cement influences pulpal healing remain unclear. We evaluated S-PRG cement-induced tertiary dentin formation in vivo, and its effect on the pulp cell healing process in vitro. Induced tertiary dentin formation was evaluated with micro-computed tomography (μCT) and scanning electron microscopy (SEM). The distribution of elements from the S-PRG cement in pulpal tissue was confirmed by micro-X-ray fluorescence (μXRF). The effects of S-PRG cement on cytotoxicity, proliferation, formation of mineralized nodules, and gene expression in human dental pulp stem cells (hDPSCs) were assessed in vitro. μCT and SEM revealed that S-PRG induced tertiary dentin formation with similar characteristics to that induced by hydraulic calcium-silicate cement (ProRoot mineral trioxide aggregate (MTA)). μXRF showed Sr and Si ion transfer into pulpal tissue from S-PRG cement. Notably, S-PRG cement and MTA showed similar biocompatibility. A co-culture of hDPSCs and S-PRG discs promoted mineralized nodule formation on surrounding cells. Additionally, S-PRG cement regulated the expression of genes related to osteo/dentinogenic differentiation. MTA and S-PRG regulated gene expression in hDPSCs, but the patterns of regulation differed. S-PRG cement upregulated
and
gene expression. These findings showed that S-PRG and MTA exhibit similar effects on dental pulp through different mechanisms.</abstract><cop>Switzerland</cop><pub>MDPI</pub><pmid>31514356</pmid><doi>10.3390/jcm8091440</doi><orcidid>https://orcid.org/0000-0002-0000-025X</orcidid><orcidid>https://orcid.org/0000-0001-6338-4767</orcidid><orcidid>https://orcid.org/0000-0002-3759-5031</orcidid><orcidid>https://orcid.org/0000-0002-4552-783X</orcidid><oa>free_for_read</oa></addata></record> |
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| title | Surface Pre-Reacted Glass Filler Contributes to Tertiary Dentin Formation through a Mechanism Different Than That of Hydraulic Calcium-Silicate Cement |
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