Xerostomia Therapy Due to Ionized Radiation Using Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells
Abstract Objectives The aim of this study was to describe the process of regeneration of damaged salivary glands due to ionizing radiations by bone marrow mesenchymal stem cells (BM-MSCs) transplantation that have been given hypoxic preconditioning with 1% O 2 concentration. Materials and Methods St...
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| Veröffentlicht in: | European journal of dentistry 2019-05, Vol.13 (2), p.238-242 |
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| container_title | European journal of dentistry |
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| creator | Mulyani, Sri Wigati Mardi Astuti, Eha Renwi Wahyuni, Otty Ratna Ernawati, Diah Savitri Ramadhani, Nastiti Faradilla |
| description | Abstract
Objectives
The aim of this study was to describe the process of regeneration of damaged salivary glands due to ionizing radiations by bone marrow mesenchymal stem cells (BM-MSCs) transplantation that have been given hypoxic preconditioning with 1% O
2
concentration.
Materials and Methods
Stem cell culture was performed under normoxic (O
2
: 21%) and hypoxic conditions by incubating the cells for 48 hours in a low oxygen tension chamber consisting of 95% N
2
, 5% CO
2
, and 1% O
2
. Thirty male Wistar rats were divided into four groups: two groups of control and two groups of treatment. A single dose of 15 Gy radiation was provided to the ventral region of the neck in all treatment groups, damaging the salivary glands. BM-MSCs transplantation was performed in the treatment groups for normoxia and hypoxia 24-hour postradiation.
Statistical Analysis
Statistical analysis was done using normality test, followed by MANOVA test (
p
< 0.05).
Results
There was a significant difference in the expression of binding SDF1-CXCR4, Bcl-2 (
p
< 0.05) and also the activity of the enzyme α-amylase in all groups of hypoxia.
Conclusion
BM-MSCs transplantation with hypoxic precondition increases the expression of binding SDF1-CXCR4, Bcl-2 that contributes to cell migration, cell survival, and cell differentiation. |
| doi_str_mv | 10.1055/s-0039-1694697 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6777157</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2289572339</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4097-65b3d6d2ee664c4ad3b29c872105339fb64df9f79f8fa4293f0010b283567f163</originalsourceid><addsrcrecordid>eNp1kUtv3CAUhVGVqnm02y4rltk4AWPAbCKlk-YhJWrVJlJ3CNuXDKkNU7ATTX59GM10lC6yAXTvx7mPg9BnSo4o4fw4FYQwVVChKqHkO7RHGeGFrES1s31zsYv2U3ogRDCh1Ae0yygnqpZiD_35DTGkMQzO4Ns5RLNY4rMJ8BjwVfDuGTr803TOjC54fJecv8c_IrTBd24Vyumv-cQ3JsbwVJxBdI85dgMJfDtfDqbHv0YY8Az6Pn1E763pE3za3Afo7vzb7eyyuP5-cTU7vS7aiihZCN6wTnQlgBBVW5mONaVqa1nmgRlTthFVZ5WVytbWVKVilhBKmrJmXEhLBTtAJ2vdxdQM0LXgx2h6vYhuMHGpg3H6_4x3c30fHrWQUlIus8DhRiCGvxOkUQ8utXkE4yFMSZdlrbgsczMZPVqjbV5jimC3ZSjRK4d00iuH9Mah_OHL6-a2-D9LMlCsgXHuYAD9EKbo87reEnwBR4mbgw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2289572339</pqid></control><display><type>article</type><title>Xerostomia Therapy Due to Ionized Radiation Using Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells</title><source>Thieme Connect Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Mulyani, Sri Wigati Mardi ; Astuti, Eha Renwi ; Wahyuni, Otty Ratna ; Ernawati, Diah Savitri ; Ramadhani, Nastiti Faradilla</creator><creatorcontrib>Mulyani, Sri Wigati Mardi ; Astuti, Eha Renwi ; Wahyuni, Otty Ratna ; Ernawati, Diah Savitri ; Ramadhani, Nastiti Faradilla</creatorcontrib><description>Abstract
Objectives
The aim of this study was to describe the process of regeneration of damaged salivary glands due to ionizing radiations by bone marrow mesenchymal stem cells (BM-MSCs) transplantation that have been given hypoxic preconditioning with 1% O
2
concentration.
Materials and Methods
Stem cell culture was performed under normoxic (O
2
: 21%) and hypoxic conditions by incubating the cells for 48 hours in a low oxygen tension chamber consisting of 95% N
2
, 5% CO
2
, and 1% O
2
. Thirty male Wistar rats were divided into four groups: two groups of control and two groups of treatment. A single dose of 15 Gy radiation was provided to the ventral region of the neck in all treatment groups, damaging the salivary glands. BM-MSCs transplantation was performed in the treatment groups for normoxia and hypoxia 24-hour postradiation.
Statistical Analysis
Statistical analysis was done using normality test, followed by MANOVA test (
p
< 0.05).
Results
There was a significant difference in the expression of binding SDF1-CXCR4, Bcl-2 (
p
< 0.05) and also the activity of the enzyme α-amylase in all groups of hypoxia.
Conclusion
BM-MSCs transplantation with hypoxic precondition increases the expression of binding SDF1-CXCR4, Bcl-2 that contributes to cell migration, cell survival, and cell differentiation.</description><identifier>ISSN: 1305-7456</identifier><identifier>EISSN: 1305-7464</identifier><identifier>DOI: 10.1055/s-0039-1694697</identifier><identifier>PMID: 31509876</identifier><language>eng</language><publisher>A-12, Second Floor, Sector -2, NOIDA -201301, India: Thieme Medical and Scientific Publishers Private Ltd</publisher><subject>Original ; Original Article</subject><ispartof>European journal of dentistry, 2019-05, Vol.13 (2), p.238-242</ispartof><rights>Dental Investigation Society.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4097-65b3d6d2ee664c4ad3b29c872105339fb64df9f79f8fa4293f0010b283567f163</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777157/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777157/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,20891,27924,27925,53791,53793,54587,54615</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31509876$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mulyani, Sri Wigati Mardi</creatorcontrib><creatorcontrib>Astuti, Eha Renwi</creatorcontrib><creatorcontrib>Wahyuni, Otty Ratna</creatorcontrib><creatorcontrib>Ernawati, Diah Savitri</creatorcontrib><creatorcontrib>Ramadhani, Nastiti Faradilla</creatorcontrib><title>Xerostomia Therapy Due to Ionized Radiation Using Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells</title><title>European journal of dentistry</title><addtitle>Eur J Dent</addtitle><description>Abstract
Objectives
The aim of this study was to describe the process of regeneration of damaged salivary glands due to ionizing radiations by bone marrow mesenchymal stem cells (BM-MSCs) transplantation that have been given hypoxic preconditioning with 1% O
2
concentration.
Materials and Methods
Stem cell culture was performed under normoxic (O
2
: 21%) and hypoxic conditions by incubating the cells for 48 hours in a low oxygen tension chamber consisting of 95% N
2
, 5% CO
2
, and 1% O
2
. Thirty male Wistar rats were divided into four groups: two groups of control and two groups of treatment. A single dose of 15 Gy radiation was provided to the ventral region of the neck in all treatment groups, damaging the salivary glands. BM-MSCs transplantation was performed in the treatment groups for normoxia and hypoxia 24-hour postradiation.
Statistical Analysis
Statistical analysis was done using normality test, followed by MANOVA test (
p
< 0.05).
Results
There was a significant difference in the expression of binding SDF1-CXCR4, Bcl-2 (
p
< 0.05) and also the activity of the enzyme α-amylase in all groups of hypoxia.
Conclusion
BM-MSCs transplantation with hypoxic precondition increases the expression of binding SDF1-CXCR4, Bcl-2 that contributes to cell migration, cell survival, and cell differentiation.</description><subject>Original</subject><subject>Original Article</subject><issn>1305-7456</issn><issn>1305-7464</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>0U6</sourceid><recordid>eNp1kUtv3CAUhVGVqnm02y4rltk4AWPAbCKlk-YhJWrVJlJ3CNuXDKkNU7ATTX59GM10lC6yAXTvx7mPg9BnSo4o4fw4FYQwVVChKqHkO7RHGeGFrES1s31zsYv2U3ogRDCh1Ae0yygnqpZiD_35DTGkMQzO4Ns5RLNY4rMJ8BjwVfDuGTr803TOjC54fJecv8c_IrTBd24Vyumv-cQ3JsbwVJxBdI85dgMJfDtfDqbHv0YY8Az6Pn1E763pE3za3Afo7vzb7eyyuP5-cTU7vS7aiihZCN6wTnQlgBBVW5mONaVqa1nmgRlTthFVZ5WVytbWVKVilhBKmrJmXEhLBTtAJ2vdxdQM0LXgx2h6vYhuMHGpg3H6_4x3c30fHrWQUlIus8DhRiCGvxOkUQ8utXkE4yFMSZdlrbgsczMZPVqjbV5jimC3ZSjRK4d00iuH9Mah_OHL6-a2-D9LMlCsgXHuYAD9EKbo87reEnwBR4mbgw</recordid><startdate>201905</startdate><enddate>201905</enddate><creator>Mulyani, Sri Wigati Mardi</creator><creator>Astuti, Eha Renwi</creator><creator>Wahyuni, Otty Ratna</creator><creator>Ernawati, Diah Savitri</creator><creator>Ramadhani, Nastiti Faradilla</creator><general>Thieme Medical and Scientific Publishers Private Ltd</general><scope>0U6</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201905</creationdate><title>Xerostomia Therapy Due to Ionized Radiation Using Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells</title><author>Mulyani, Sri Wigati Mardi ; Astuti, Eha Renwi ; Wahyuni, Otty Ratna ; Ernawati, Diah Savitri ; Ramadhani, Nastiti Faradilla</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4097-65b3d6d2ee664c4ad3b29c872105339fb64df9f79f8fa4293f0010b283567f163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Original</topic><topic>Original Article</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mulyani, Sri Wigati Mardi</creatorcontrib><creatorcontrib>Astuti, Eha Renwi</creatorcontrib><creatorcontrib>Wahyuni, Otty Ratna</creatorcontrib><creatorcontrib>Ernawati, Diah Savitri</creatorcontrib><creatorcontrib>Ramadhani, Nastiti Faradilla</creatorcontrib><collection>Thieme Connect Journals Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>European journal of dentistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mulyani, Sri Wigati Mardi</au><au>Astuti, Eha Renwi</au><au>Wahyuni, Otty Ratna</au><au>Ernawati, Diah Savitri</au><au>Ramadhani, Nastiti Faradilla</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Xerostomia Therapy Due to Ionized Radiation Using Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells</atitle><jtitle>European journal of dentistry</jtitle><addtitle>Eur J Dent</addtitle><date>2019-05</date><risdate>2019</risdate><volume>13</volume><issue>2</issue><spage>238</spage><epage>242</epage><pages>238-242</pages><issn>1305-7456</issn><eissn>1305-7464</eissn><abstract>Abstract
Objectives
The aim of this study was to describe the process of regeneration of damaged salivary glands due to ionizing radiations by bone marrow mesenchymal stem cells (BM-MSCs) transplantation that have been given hypoxic preconditioning with 1% O
2
concentration.
Materials and Methods
Stem cell culture was performed under normoxic (O
2
: 21%) and hypoxic conditions by incubating the cells for 48 hours in a low oxygen tension chamber consisting of 95% N
2
, 5% CO
2
, and 1% O
2
. Thirty male Wistar rats were divided into four groups: two groups of control and two groups of treatment. A single dose of 15 Gy radiation was provided to the ventral region of the neck in all treatment groups, damaging the salivary glands. BM-MSCs transplantation was performed in the treatment groups for normoxia and hypoxia 24-hour postradiation.
Statistical Analysis
Statistical analysis was done using normality test, followed by MANOVA test (
p
< 0.05).
Results
There was a significant difference in the expression of binding SDF1-CXCR4, Bcl-2 (
p
< 0.05) and also the activity of the enzyme α-amylase in all groups of hypoxia.
Conclusion
BM-MSCs transplantation with hypoxic precondition increases the expression of binding SDF1-CXCR4, Bcl-2 that contributes to cell migration, cell survival, and cell differentiation.</abstract><cop>A-12, Second Floor, Sector -2, NOIDA -201301, India</cop><pub>Thieme Medical and Scientific Publishers Private Ltd</pub><pmid>31509876</pmid><doi>10.1055/s-0039-1694697</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | Thieme Connect Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Original Original Article |
| title | Xerostomia Therapy Due to Ionized Radiation Using Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells |
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