Phosphorylation-Mediated Dynamics of Nitrate Transceptor NRT1.1 Regulate Auxin Flux and Nitrate Signaling in Lateral Root Growth1[OPEN]

Phosphorylation-Mediated Dynamics of Nitrate Transceptor NRT1.1 Regulate Auxin Flux and Nitrate Signaling in Lateral Root Growth1[OPEN]

The differential phosphorylation of nitrate transceptor NRT1.1 contributes to the modulation of lateral root development through spatiotemporal plasma membrane dynamics and cellular trafficking. The dual-affinity nitrate transceptor NITRATE TRANSPORTER1.1 (NRT1.1) has two modes of transport and sign...

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Veröffentlicht in:Plant physiology (Bethesda) 2019-08, Vol.181 (2), p.480-498
Hauptverfasser: Zhang, Xi, Cui, Yaning, Yu, Meng, Su, Bodan, Gong, Wei, Baluška, František, Komis, George, Šamaj, Jozef, Shan, Xiaoyi, Lin, Jinxing
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container_issue 2
container_start_page 480
container_title Plant physiology (Bethesda)
container_volume 181
creator Zhang, Xi
Cui, Yaning
Yu, Meng
Su, Bodan
Gong, Wei
Baluška, František
Komis, George
Šamaj, Jozef
Shan, Xiaoyi
Lin, Jinxing
description The differential phosphorylation of nitrate transceptor NRT1.1 contributes to the modulation of lateral root development through spatiotemporal plasma membrane dynamics and cellular trafficking. The dual-affinity nitrate transceptor NITRATE TRANSPORTER1.1 (NRT1.1) has two modes of transport and signaling, governed by Thr-101 (T101) phosphorylation. NRT1.1 regulates lateral root (LR) development by modulating nitrate-dependent basipetal auxin export and nitrate-mediated signal transduction. Here, using the Arabidopsis ( Arabidopsis thaliana ) NRT1.1 T101D phosphomimetic and NRT1.1 T101A nonphosphorylatable mutants, we found that the phosphorylation state of NRT1.1 plays a key role in NRT1.1 function during LR development. Single-particle tracking revealed that phosphorylation affected NRT1.1 spatiotemporal dynamics. The phosphomimetic NRT1.1 T101D form showed fast lateral mobility and membrane partitioning that facilitated auxin flux under low-nitrate conditions. By contrast, nonphosphorylatable NRT1.1 T101A showed low lateral mobility and oligomerized at the plasma membrane (PM), where it induced endocytosis via the clathrin-mediated endocytosis and microdomain-mediated endocytosis pathways under high-nitrate conditions. These behaviors promoted LR development by suppressing NRT1.1-controlled auxin transport on the PM and stimulating Ca 2+ -ARABIDOPSIS NITRATE REGULATED1 signaling from the endosome.
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title Phosphorylation-Mediated Dynamics of Nitrate Transceptor NRT1.1 Regulate Auxin Flux and Nitrate Signaling in Lateral Root Growth1[OPEN]
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