LncRNA RHPN1-AS1 Targeting miR-625/REG3A Promotes Cell Proliferation And Invasion Of Glioma Cells
Glioma arises from the proliferation of neuroglial cells differentiated from the ectoderm. Evidence has confirmed that differentially expressed long non-coding RNAs (lncRNAs) may be involved in the development and progression of various tumors. The present study aimed to explore the biological funct...
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| Veröffentlicht in: | OncoTargets and therapy 2019-01, Vol.12, p.7911-7921 |
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| creator | Cui, Peng Su, Jichun Li, Qingmin Xu, Guangming Zhu, Ningxi |
| description | Glioma arises from the proliferation of neuroglial cells differentiated from the ectoderm. Evidence has confirmed that differentially expressed long non-coding RNAs (lncRNAs) may be involved in the development and progression of various tumors. The present study aimed to explore the biological function of lncRNA RHPN1-AS1 in glioma.
The expressions of RHPN1-AS1 in glioma tissues and cells were examined using RT-PCR. Colony formation assay, MTT assay, wound healing assay and transwell assay were performed to detect cell cloning efficiency, proliferation, migration and invasion of glioma cells, respectively. Western blot was applied to assess the expression levels of migration-related and invasion-related proteins. Online bioinformatic tools and luciferase reporter assay were, respectively, employed to predict and verify the downstream target microRNA/gene of RHPN1-AS1.
RHPN1-AS1 was up-regulated in glioma tissues and cells. The cell proliferation, migration and invasion of glioma were inhibited when the expression of RHPN1-AS1 was down-regulated in glioma cells. The expressions of migration-related and invasion-related proteins were also suppressed in siRHPN1-AS1 groups. Furthermore, we predicted and verified that RHPN1-AS1 was directly targeted to miR-625-5p/REG3A. Our study demonstrated that the knockdown of RHPN1-AS1 inhibited the proliferation, migration and invasion activity of glioma cells via regulating miR-625-5p/REG3A expression.
The results revealed that the lncRNA RHPN1-AS1 may be a molecular target in glioma therapy. |
| doi_str_mv | 10.2147/OTT.S209563 |
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The expressions of RHPN1-AS1 in glioma tissues and cells were examined using RT-PCR. Colony formation assay, MTT assay, wound healing assay and transwell assay were performed to detect cell cloning efficiency, proliferation, migration and invasion of glioma cells, respectively. Western blot was applied to assess the expression levels of migration-related and invasion-related proteins. Online bioinformatic tools and luciferase reporter assay were, respectively, employed to predict and verify the downstream target microRNA/gene of RHPN1-AS1.
RHPN1-AS1 was up-regulated in glioma tissues and cells. The cell proliferation, migration and invasion of glioma were inhibited when the expression of RHPN1-AS1 was down-regulated in glioma cells. The expressions of migration-related and invasion-related proteins were also suppressed in siRHPN1-AS1 groups. Furthermore, we predicted and verified that RHPN1-AS1 was directly targeted to miR-625-5p/REG3A. Our study demonstrated that the knockdown of RHPN1-AS1 inhibited the proliferation, migration and invasion activity of glioma cells via regulating miR-625-5p/REG3A expression.
The results revealed that the lncRNA RHPN1-AS1 may be a molecular target in glioma therapy.</description><identifier>ISSN: 1178-6930</identifier><identifier>EISSN: 1178-6930</identifier><identifier>DOI: 10.2147/OTT.S209563</identifier><identifier>PMID: 31576148</identifier><language>eng</language><publisher>New Zealand: Taylor & Francis Ltd</publisher><subject>Apoptosis ; Brain tumors ; Cancer therapies ; Cell adhesion & migration ; Cell differentiation ; Cell growth ; Cell migration ; Cell proliferation ; Chemotherapy ; Colorectal cancer ; Ectoderm ; Gastric cancer ; Gene expression ; Glial cells ; Glioma ; Glioma cells ; Hospitals ; Lung cancer ; Metastasis ; MicroRNAs ; miRNA ; Neurosurgery ; Non-coding RNA ; Original Research ; Pathogenesis ; Polymerase chain reaction ; RNA polymerase ; Stem cells ; Studies ; Tumors ; Wound healing</subject><ispartof>OncoTargets and therapy, 2019-01, Vol.12, p.7911-7921</ispartof><rights>2019 Cui et al.</rights><rights>2019. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Cui et al. 2019 Cui et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-f6b5760761abbe8c92c0872729b5aa8136a8715bd258c2caca291424dcff6fde3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769163/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769163/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,3849,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31576148$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cui, Peng</creatorcontrib><creatorcontrib>Su, Jichun</creatorcontrib><creatorcontrib>Li, Qingmin</creatorcontrib><creatorcontrib>Xu, Guangming</creatorcontrib><creatorcontrib>Zhu, Ningxi</creatorcontrib><title>LncRNA RHPN1-AS1 Targeting miR-625/REG3A Promotes Cell Proliferation And Invasion Of Glioma Cells</title><title>OncoTargets and therapy</title><addtitle>Onco Targets Ther</addtitle><description>Glioma arises from the proliferation of neuroglial cells differentiated from the ectoderm. Evidence has confirmed that differentially expressed long non-coding RNAs (lncRNAs) may be involved in the development and progression of various tumors. The present study aimed to explore the biological function of lncRNA RHPN1-AS1 in glioma.
The expressions of RHPN1-AS1 in glioma tissues and cells were examined using RT-PCR. Colony formation assay, MTT assay, wound healing assay and transwell assay were performed to detect cell cloning efficiency, proliferation, migration and invasion of glioma cells, respectively. Western blot was applied to assess the expression levels of migration-related and invasion-related proteins. Online bioinformatic tools and luciferase reporter assay were, respectively, employed to predict and verify the downstream target microRNA/gene of RHPN1-AS1.
RHPN1-AS1 was up-regulated in glioma tissues and cells. The cell proliferation, migration and invasion of glioma were inhibited when the expression of RHPN1-AS1 was down-regulated in glioma cells. The expressions of migration-related and invasion-related proteins were also suppressed in siRHPN1-AS1 groups. Furthermore, we predicted and verified that RHPN1-AS1 was directly targeted to miR-625-5p/REG3A. Our study demonstrated that the knockdown of RHPN1-AS1 inhibited the proliferation, migration and invasion activity of glioma cells via regulating miR-625-5p/REG3A expression.
The results revealed that the lncRNA RHPN1-AS1 may be a molecular target in glioma therapy.</description><subject>Apoptosis</subject><subject>Brain tumors</subject><subject>Cancer therapies</subject><subject>Cell adhesion & migration</subject><subject>Cell differentiation</subject><subject>Cell growth</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Chemotherapy</subject><subject>Colorectal cancer</subject><subject>Ectoderm</subject><subject>Gastric cancer</subject><subject>Gene expression</subject><subject>Glial cells</subject><subject>Glioma</subject><subject>Glioma cells</subject><subject>Hospitals</subject><subject>Lung cancer</subject><subject>Metastasis</subject><subject>MicroRNAs</subject><subject>miRNA</subject><subject>Neurosurgery</subject><subject>Non-coding RNA</subject><subject>Original Research</subject><subject>Pathogenesis</subject><subject>Polymerase chain reaction</subject><subject>RNA polymerase</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Tumors</subject><subject>Wound healing</subject><issn>1178-6930</issn><issn>1178-6930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc1LxDAQxYMofqyevEvBiyBd89Em6UUoi67C4sq6nkOapmukTTTpCv73prqKepoZ5sfjzTwAjhEcY5Sxi_lyOX7AsMgp2QL7CDGe0oLA7V_9HjgI4RlCSjnOdsEeQTmjKOP7QM6sWtyVyeLm_g6l5QNKltKvdG_sKunMIqU4v1hcTUmZ3HvXuV6HZKLbdpha02gve-NsUto6ubVvMgzDvEmmrXGd_CTDIdhpZBv00aaOwOP11XJyk87m09tJOUtVBos-bWgVPcFoS1aV5qrACnKGGS6qXEqOCJWcobyqcc4VVlJJXKAMZ7VqGtrUmozA5Zfuy7rqdK207b1sxYs3nfTvwkkj_m6seRIr9yYoowWiJAqcbQS8e13r0IvOBBVPkFa7dRCYQBgfyvmAnv5Dn93a23jeQFHMMSMsUudflPIuBK-bHzMIiiE6EaMTm-giffLb_w_7nRX5AIEzkfI</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Cui, Peng</creator><creator>Su, Jichun</creator><creator>Li, Qingmin</creator><creator>Xu, Guangming</creator><creator>Zhu, Ningxi</creator><general>Taylor & Francis Ltd</general><general>Dove</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190101</creationdate><title>LncRNA RHPN1-AS1 Targeting miR-625/REG3A Promotes Cell Proliferation And Invasion Of Glioma Cells</title><author>Cui, Peng ; Su, Jichun ; Li, Qingmin ; Xu, Guangming ; Zhu, Ningxi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-f6b5760761abbe8c92c0872729b5aa8136a8715bd258c2caca291424dcff6fde3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Apoptosis</topic><topic>Brain tumors</topic><topic>Cancer therapies</topic><topic>Cell adhesion & migration</topic><topic>Cell differentiation</topic><topic>Cell growth</topic><topic>Cell migration</topic><topic>Cell proliferation</topic><topic>Chemotherapy</topic><topic>Colorectal cancer</topic><topic>Ectoderm</topic><topic>Gastric cancer</topic><topic>Gene expression</topic><topic>Glial cells</topic><topic>Glioma</topic><topic>Glioma cells</topic><topic>Hospitals</topic><topic>Lung cancer</topic><topic>Metastasis</topic><topic>MicroRNAs</topic><topic>miRNA</topic><topic>Neurosurgery</topic><topic>Non-coding RNA</topic><topic>Original Research</topic><topic>Pathogenesis</topic><topic>Polymerase chain reaction</topic><topic>RNA polymerase</topic><topic>Stem cells</topic><topic>Studies</topic><topic>Tumors</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cui, Peng</creatorcontrib><creatorcontrib>Su, Jichun</creatorcontrib><creatorcontrib>Li, Qingmin</creatorcontrib><creatorcontrib>Xu, Guangming</creatorcontrib><creatorcontrib>Zhu, Ningxi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>OncoTargets and therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cui, Peng</au><au>Su, Jichun</au><au>Li, Qingmin</au><au>Xu, Guangming</au><au>Zhu, Ningxi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LncRNA RHPN1-AS1 Targeting miR-625/REG3A Promotes Cell Proliferation And Invasion Of Glioma Cells</atitle><jtitle>OncoTargets and therapy</jtitle><addtitle>Onco Targets Ther</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>12</volume><spage>7911</spage><epage>7921</epage><pages>7911-7921</pages><issn>1178-6930</issn><eissn>1178-6930</eissn><abstract>Glioma arises from the proliferation of neuroglial cells differentiated from the ectoderm. Evidence has confirmed that differentially expressed long non-coding RNAs (lncRNAs) may be involved in the development and progression of various tumors. The present study aimed to explore the biological function of lncRNA RHPN1-AS1 in glioma.
The expressions of RHPN1-AS1 in glioma tissues and cells were examined using RT-PCR. Colony formation assay, MTT assay, wound healing assay and transwell assay were performed to detect cell cloning efficiency, proliferation, migration and invasion of glioma cells, respectively. Western blot was applied to assess the expression levels of migration-related and invasion-related proteins. Online bioinformatic tools and luciferase reporter assay were, respectively, employed to predict and verify the downstream target microRNA/gene of RHPN1-AS1.
RHPN1-AS1 was up-regulated in glioma tissues and cells. The cell proliferation, migration and invasion of glioma were inhibited when the expression of RHPN1-AS1 was down-regulated in glioma cells. The expressions of migration-related and invasion-related proteins were also suppressed in siRHPN1-AS1 groups. Furthermore, we predicted and verified that RHPN1-AS1 was directly targeted to miR-625-5p/REG3A. Our study demonstrated that the knockdown of RHPN1-AS1 inhibited the proliferation, migration and invasion activity of glioma cells via regulating miR-625-5p/REG3A expression.
The results revealed that the lncRNA RHPN1-AS1 may be a molecular target in glioma therapy.</abstract><cop>New Zealand</cop><pub>Taylor & Francis Ltd</pub><pmid>31576148</pmid><doi>10.2147/OTT.S209563</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | Taylor & Francis Open Access; DOVE Medical Press Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Apoptosis Brain tumors Cancer therapies Cell adhesion & migration Cell differentiation Cell growth Cell migration Cell proliferation Chemotherapy Colorectal cancer Ectoderm Gastric cancer Gene expression Glial cells Glioma Glioma cells Hospitals Lung cancer Metastasis MicroRNAs miRNA Neurosurgery Non-coding RNA Original Research Pathogenesis Polymerase chain reaction RNA polymerase Stem cells Studies Tumors Wound healing |
| title | LncRNA RHPN1-AS1 Targeting miR-625/REG3A Promotes Cell Proliferation And Invasion Of Glioma Cells |
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