Purification of Polyglutamine Aggregates and Identification of Elongation Factor-1α and Heat Shock Protein 84 as Aggregate-Interacting Proteins

Aggregates of green fluorescent protein (GFP)-fused truncated N-terminal huntingtin containing abnormally long polyglutamine tracts (150 repeats of glutamine residue) were purified from an ecdysone-inducible mutant neuro2A cell line (HD150Q-28) by using a fluorescence-activated cell sorter. To analy...

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Veröffentlicht in:	The Journal of neuroscience 2002-11, Vol.22 (21), p.9267-9277
Hauptverfasser:	Mitsui, Kenichi, Nakayama, Hiroshi, Akagi, Takumi, Nekooki, Munenori, Ohtawa, Kenji, Takio, Koji, Hashikawa, Tsutomu, Nukina, Nobuyuki
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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