Directional Guidance of Oligodendroglial Migration by Class 3 Semaphorins and Netrin-1

Oligodendrocytes, the myelin-forming cells of the CNS, are generated from multiple foci distributed along the developing neural tube. Little is known about the endogenous guidance cues controlling the migration of oligodendrocyte precursor cells (OPCs) from their site of emergence toward their final...

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Veröffentlicht in:The Journal of neuroscience 2002-07, Vol.22 (14), p.5992-6004
Hauptverfasser: Spassky, Nathalie, de Castro, Fernando, Le Bras, Barbara, Heydon, Katharina, Queraud-LeSaux, Francoise, Bloch-Gallego, Evelyne, Chedotal, Alain, Zalc, Bernard, Thomas, Jean-Leon
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container_end_page 6004
container_issue 14
container_start_page 5992
container_title The Journal of neuroscience
container_volume 22
creator Spassky, Nathalie
de Castro, Fernando
Le Bras, Barbara
Heydon, Katharina
Queraud-LeSaux, Francoise
Bloch-Gallego, Evelyne
Chedotal, Alain
Zalc, Bernard
Thomas, Jean-Leon
description Oligodendrocytes, the myelin-forming cells of the CNS, are generated from multiple foci distributed along the developing neural tube. Little is known about the endogenous guidance cues controlling the migration of oligodendrocyte precursor cells (OPCs) from their site of emergence toward their final destination, mainly the future white matter tracts. During embryonic development, the optic nerve is populated by OPCs originating in the diencephalon that migrate from the chiasm toward the retina. Here we show that OPCs migrating into the embryonic optic nerve express the semaphorin receptors neuropilin-1 and -2, as well as deleted in colorectal cancer (DCC) and, to a lesser extend unc5H1, two of the netrin-1 receptors. Using a functional migration assay, we provide evidence that Sema 3A and netrin-1 exert opposite chemotactic effects, repulsive or attractive, respectively, on embryonic OPCs. In addition, we show that Sema 3F has a dual effect, chemoattractive and mitogenic on embryonic OPCs. The localization of cells expressing Sema 3A, Sema 3F, and netrin-1 is consistent with a role for these ligands in the migration of OPCs in the embryonic optic nerve. Altogether, our results suggest that the migration of OPCs in the embryonic optic nerve is modulated by a balance of effects mediated by members of the semaphorin and netrin families.
doi_str_mv 10.1523/jneurosci.22-14-05992.2002
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The localization of cells expressing Sema 3A, Sema 3F, and netrin-1 is consistent with a role for these ligands in the migration of OPCs in the embryonic optic nerve. Altogether, our results suggest that the migration of OPCs in the embryonic optic nerve is modulated by a balance of effects mediated by members of the semaphorin and netrin families.</abstract><cop>United States</cop><pub>Soc Neuroscience</pub><pmid>12122061</pmid><doi>10.1523/jneurosci.22-14-05992.2002</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-3397-0292</orcidid><orcidid>https://orcid.org/0000-0001-7577-3794</orcidid><oa>free_for_read</oa></addata></record>
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subjects Animals
Cell Adhesion Molecules - biosynthesis
Cell Division - drug effects
Cell Division - physiology
Cell Line
Cell Lineage
Cell Movement - drug effects
Cell Movement - physiology
Chemotaxis - drug effects
Chemotaxis - physiology
Culture Techniques
DCC Receptor
Glycoproteins - metabolism
Glycoproteins - pharmacology
Humans
Life Sciences
Membrane Proteins - metabolism
Membrane Proteins - pharmacology
Mice
Nerve Growth Factors - metabolism
Nerve Growth Factors - pharmacology
Nerve Tissue Proteins - biosynthesis
Nerve Tissue Proteins - metabolism
Nerve Tissue Proteins - pharmacology
Netrin Receptors
Netrin-1
Neurons and Cognition
Neuropilin-1
Oligodendroglia - cytology
Oligodendroglia - drug effects
Oligodendroglia - metabolism
Optic Nerve - cytology
Optic Nerve - embryology
Optic Nerve - metabolism
Receptors, Cell Surface - biosynthesis
Semaphorin-3A
Stem Cells - cytology
Stem Cells - drug effects
Stem Cells - metabolism
Tumor Suppressor Proteins - biosynthesis
title Directional Guidance of Oligodendroglial Migration by Class 3 Semaphorins and Netrin-1
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