Regional Differences in Distribution and Functional Expression of Small-Conductance Ca2+-Activated K+ Channels in Rat Brain
Small-conductance Ca2+-activated K+ (SK) channels are important for excitability control and afterhyperpolarizations in vertebrate neurons and have been implicated in regulation of the functional state of the forebrain. We have examined the distribution, functional expression, and subunit compositio...
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| Veröffentlicht in: | The Journal of neuroscience 2002-11, Vol.22 (22), p.9698-9707 |
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| container_title | The Journal of neuroscience |
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| creator | Sailer, Claudia A Hu, Hua Kaufmann, Walter A Trieb, Maria Schwarzer, Christoph Storm, Johan F Knaus, Hans-Gunther |
| description | Small-conductance Ca2+-activated K+ (SK) channels are important for excitability control and afterhyperpolarizations in vertebrate neurons and have been implicated in regulation of the functional state of the forebrain. We have examined the distribution, functional expression, and subunit composition of SK channels in rat brain. Immunoprecipitation detected solely homotetrameric SK2 and SK3 channels in native tissue and their constitutive association with calmodulin. Immunohistochemistry revealed a restricted distribution of SK1 and SK2 protein with highest densities in subregions of the hippocampus and neocortex. In contrast, SK3 protein was distributed more diffusely in these brain regions and predominantly expressed in phylogenetically older brain regions. Whole-cell recording showed a sharp segregation of apamin-sensitive SK current within the hippocampal formation, in agreement with the SK2 distribution, suggesting that SK2 homotetramers underlie the apamin-sensitive medium afterhyperpolarizations in rat hippocampus. |
| doi_str_mv | 10.1523/jneurosci.22-22-09698.2002 |
| format | Article |
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We have examined the distribution, functional expression, and subunit composition of SK channels in rat brain. Immunoprecipitation detected solely homotetrameric SK2 and SK3 channels in native tissue and their constitutive association with calmodulin. Immunohistochemistry revealed a restricted distribution of SK1 and SK2 protein with highest densities in subregions of the hippocampus and neocortex. In contrast, SK3 protein was distributed more diffusely in these brain regions and predominantly expressed in phylogenetically older brain regions. Whole-cell recording showed a sharp segregation of apamin-sensitive SK current within the hippocampal formation, in agreement with the SK2 distribution, suggesting that SK2 homotetramers underlie the apamin-sensitive medium afterhyperpolarizations in rat hippocampus.</description><identifier>ISSN: 0270-6474</identifier><identifier>EISSN: 1529-2401</identifier><identifier>DOI: 10.1523/jneurosci.22-22-09698.2002</identifier><identifier>PMID: 12427825</identifier><language>eng</language><publisher>United States: Soc Neuroscience</publisher><subject>Animals ; Antibody Specificity ; Apamin - pharmacokinetics ; Binding, Competitive - physiology ; Brain - cytology ; Brain - metabolism ; Brain Chemistry ; Calmodulin - metabolism ; Cell Membrane - chemistry ; Cell Membrane - metabolism ; Cyclic AMP - metabolism ; Hippocampus - chemistry ; Hippocampus - cytology ; Hippocampus - metabolism ; Immunohistochemistry ; In Vitro Techniques ; Neocortex - chemistry ; Neocortex - cytology ; Neocortex - metabolism ; Oocytes - chemistry ; Oocytes - metabolism ; Organ Specificity - physiology ; Patch-Clamp Techniques ; Potassium Channels - analysis ; Potassium Channels - metabolism ; Potassium Channels, Calcium-Activated ; Precipitin Tests ; Rats ; Rats, Sprague-Dawley ; Small-Conductance Calcium-Activated Potassium Channels ; Xenopus</subject><ispartof>The Journal of neuroscience, 2002-11, Vol.22 (22), p.9698-9707</ispartof><rights>Copyright © 2002 Society for Neuroscience 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-a3b88d19fb4fe2590c65e8a675be4f6f06b628d6970dc0f19f7202dbe1e61e653</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757844/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757844/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12427825$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sailer, Claudia A</creatorcontrib><creatorcontrib>Hu, Hua</creatorcontrib><creatorcontrib>Kaufmann, Walter A</creatorcontrib><creatorcontrib>Trieb, Maria</creatorcontrib><creatorcontrib>Schwarzer, Christoph</creatorcontrib><creatorcontrib>Storm, Johan F</creatorcontrib><creatorcontrib>Knaus, Hans-Gunther</creatorcontrib><title>Regional Differences in Distribution and Functional Expression of Small-Conductance Ca2+-Activated K+ Channels in Rat Brain</title><title>The Journal of neuroscience</title><addtitle>J Neurosci</addtitle><description>Small-conductance Ca2+-activated K+ (SK) channels are important for excitability control and afterhyperpolarizations in vertebrate neurons and have been implicated in regulation of the functional state of the forebrain. We have examined the distribution, functional expression, and subunit composition of SK channels in rat brain. Immunoprecipitation detected solely homotetrameric SK2 and SK3 channels in native tissue and their constitutive association with calmodulin. Immunohistochemistry revealed a restricted distribution of SK1 and SK2 protein with highest densities in subregions of the hippocampus and neocortex. In contrast, SK3 protein was distributed more diffusely in these brain regions and predominantly expressed in phylogenetically older brain regions. Whole-cell recording showed a sharp segregation of apamin-sensitive SK current within the hippocampal formation, in agreement with the SK2 distribution, suggesting that SK2 homotetramers underlie the apamin-sensitive medium afterhyperpolarizations in rat hippocampus.</description><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Apamin - pharmacokinetics</subject><subject>Binding, Competitive - physiology</subject><subject>Brain - cytology</subject><subject>Brain - metabolism</subject><subject>Brain Chemistry</subject><subject>Calmodulin - metabolism</subject><subject>Cell Membrane - chemistry</subject><subject>Cell Membrane - metabolism</subject><subject>Cyclic AMP - metabolism</subject><subject>Hippocampus - chemistry</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - metabolism</subject><subject>Immunohistochemistry</subject><subject>In Vitro Techniques</subject><subject>Neocortex - chemistry</subject><subject>Neocortex - cytology</subject><subject>Neocortex - metabolism</subject><subject>Oocytes - chemistry</subject><subject>Oocytes - metabolism</subject><subject>Organ Specificity - physiology</subject><subject>Patch-Clamp Techniques</subject><subject>Potassium Channels - analysis</subject><subject>Potassium Channels - metabolism</subject><subject>Potassium Channels, Calcium-Activated</subject><subject>Precipitin Tests</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Small-Conductance Calcium-Activated Potassium Channels</subject><subject>Xenopus</subject><issn>0270-6474</issn><issn>1529-2401</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkVFrFDEUhYNU2rX2L0jwwT6UWTN3Z5LMS6EdWxULQtXnkMnc7KbMZrZJpqv45826tVg4EML5cg73hpC3JZuXNSze33mcwhiNmwMUWazhjZwDY_CCzDLRFFCx8oDMGAhW8EpUR-RVjHeMMcFKcUiOSqhASKhn5PctLt3o9UA_OGsxoDcYqfP5GlNw3ZSyS7Xv6fXkTdqjVz83AWPcOaOl39Z6GIp29P1kks7vaavhrLjI9INO2NMvZ7Rdae9x-Jt8qxO9DNr51-Sl1UPEk8fzmPy4vvrefipuvn783F7cFCaPkQq96KTsy8Z2lUWoG2Z4jVJzUXdYWW4Z7zjInjeC9YbZDApg0HdYIs-qF8fkfJ-7mbo19gZ9CnpQm-DWOvxSo3bquePdSi3HB5UrhKyqHPDuMSCM9xPGpNYuGhwG7XGcohLAJTRSZvDN_01PFf_2nYHTPbByy9XWBVRxt72Ml2q73QKorN13Lv4AotqVng</recordid><startdate>20021115</startdate><enddate>20021115</enddate><creator>Sailer, Claudia A</creator><creator>Hu, Hua</creator><creator>Kaufmann, Walter A</creator><creator>Trieb, Maria</creator><creator>Schwarzer, Christoph</creator><creator>Storm, Johan F</creator><creator>Knaus, Hans-Gunther</creator><general>Soc Neuroscience</general><general>Society for Neuroscience</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20021115</creationdate><title>Regional Differences in Distribution and Functional Expression of Small-Conductance Ca2+-Activated K+ Channels in Rat Brain</title><author>Sailer, Claudia A ; Hu, Hua ; Kaufmann, Walter A ; Trieb, Maria ; Schwarzer, Christoph ; Storm, Johan F ; Knaus, Hans-Gunther</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-a3b88d19fb4fe2590c65e8a675be4f6f06b628d6970dc0f19f7202dbe1e61e653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Apamin - pharmacokinetics</topic><topic>Binding, Competitive - physiology</topic><topic>Brain - cytology</topic><topic>Brain - metabolism</topic><topic>Brain Chemistry</topic><topic>Calmodulin - metabolism</topic><topic>Cell Membrane - chemistry</topic><topic>Cell Membrane - metabolism</topic><topic>Cyclic AMP - metabolism</topic><topic>Hippocampus - chemistry</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - metabolism</topic><topic>Immunohistochemistry</topic><topic>In Vitro Techniques</topic><topic>Neocortex - chemistry</topic><topic>Neocortex - cytology</topic><topic>Neocortex - metabolism</topic><topic>Oocytes - chemistry</topic><topic>Oocytes - metabolism</topic><topic>Organ Specificity - physiology</topic><topic>Patch-Clamp Techniques</topic><topic>Potassium Channels - analysis</topic><topic>Potassium Channels - metabolism</topic><topic>Potassium Channels, Calcium-Activated</topic><topic>Precipitin Tests</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Small-Conductance Calcium-Activated Potassium Channels</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sailer, Claudia A</creatorcontrib><creatorcontrib>Hu, Hua</creatorcontrib><creatorcontrib>Kaufmann, Walter A</creatorcontrib><creatorcontrib>Trieb, Maria</creatorcontrib><creatorcontrib>Schwarzer, Christoph</creatorcontrib><creatorcontrib>Storm, Johan F</creatorcontrib><creatorcontrib>Knaus, Hans-Gunther</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sailer, Claudia A</au><au>Hu, Hua</au><au>Kaufmann, Walter A</au><au>Trieb, Maria</au><au>Schwarzer, Christoph</au><au>Storm, Johan F</au><au>Knaus, Hans-Gunther</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regional Differences in Distribution and Functional Expression of Small-Conductance Ca2+-Activated K+ Channels in Rat Brain</atitle><jtitle>The Journal of neuroscience</jtitle><addtitle>J Neurosci</addtitle><date>2002-11-15</date><risdate>2002</risdate><volume>22</volume><issue>22</issue><spage>9698</spage><epage>9707</epage><pages>9698-9707</pages><issn>0270-6474</issn><eissn>1529-2401</eissn><abstract>Small-conductance Ca2+-activated K+ (SK) channels are important for excitability control and afterhyperpolarizations in vertebrate neurons and have been implicated in regulation of the functional state of the forebrain. We have examined the distribution, functional expression, and subunit composition of SK channels in rat brain. Immunoprecipitation detected solely homotetrameric SK2 and SK3 channels in native tissue and their constitutive association with calmodulin. Immunohistochemistry revealed a restricted distribution of SK1 and SK2 protein with highest densities in subregions of the hippocampus and neocortex. In contrast, SK3 protein was distributed more diffusely in these brain regions and predominantly expressed in phylogenetically older brain regions. 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| subjects | Animals Antibody Specificity Apamin - pharmacokinetics Binding, Competitive - physiology Brain - cytology Brain - metabolism Brain Chemistry Calmodulin - metabolism Cell Membrane - chemistry Cell Membrane - metabolism Cyclic AMP - metabolism Hippocampus - chemistry Hippocampus - cytology Hippocampus - metabolism Immunohistochemistry In Vitro Techniques Neocortex - chemistry Neocortex - cytology Neocortex - metabolism Oocytes - chemistry Oocytes - metabolism Organ Specificity - physiology Patch-Clamp Techniques Potassium Channels - analysis Potassium Channels - metabolism Potassium Channels, Calcium-Activated Precipitin Tests Rats Rats, Sprague-Dawley Small-Conductance Calcium-Activated Potassium Channels Xenopus |
| title | Regional Differences in Distribution and Functional Expression of Small-Conductance Ca2+-Activated K+ Channels in Rat Brain |
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