Potent modulation of the CepR quorum sensing receptor and virulence in a Burkholderia cepacia complex member using non-native lactone ligands
The Burkholderia cepacia complex (Bcc) is a family of closely related bacterial pathogens that are the causative agent of deadly human infections. Virulence in Bcc species has been shown to be controlled by the CepI/CepR quorum sensing (QS) system, which is mediated by an N -acyl L-homoserine lacton...
Gespeichert in:
Veröffentlicht in: | Scientific reports 2019-09, Vol.9 (1), p.13449-12, Article 13449 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 12 |
---|---|
container_issue | 1 |
container_start_page | 13449 |
container_title | Scientific reports |
container_volume | 9 |
creator | Slinger, Betty L. Deay, Jacqueline J. Chandler, Josephine R. Blackwell, Helen E. |
description | The
Burkholderia cepacia
complex (Bcc) is a family of closely related bacterial pathogens that are the causative agent of deadly human infections. Virulence in Bcc species has been shown to be controlled by the CepI/CepR quorum sensing (QS) system, which is mediated by an
N
-acyl L-homoserine lactone (AHL) signal (C
8
-AHL) and its cognate LuxR-type receptor (CepR). Chemical strategies to block QS in Bcc members would represent an approach to intercept this bacterial communication process and further delineate its role in infection. In the current study, we sought to identify non-native AHLs capable of agonizing or antagonizing CepR, and thereby QS, in a Bcc member. We screened a library of AHL analogs in cell-based reporters for CepR, and identified numerous highly potent CepR agonists and antagonists. These compounds remain active in a Bcc member,
B
.
multivorans
, with one agonist 250-fold more potent than the native ligand C
8
-AHL, and can affect QS-controlled motility. Further, the CepR antagonists prolong
C
.
elegans
survival in an infection model. These AHL analogs are the first reported non-native molecules that both directly modulate CepR and impact QS-controlled phenotypes in a Bcc member, and represent valuable chemical tools to assess the role of QS in Bcc infections. |
doi_str_mv | 10.1038/s41598-019-49693-x |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6748986</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2292053639</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-c86239d47b078f19b31c55bf7c8af87f259b22affd39004eb0dccf8390a8b11c3</originalsourceid><addsrcrecordid>eNp9kU1v1DAQhiNERau2f4ADssSFS8BfSewLEqzKh1SpCMHZcpzxrktip3a8Wn4E_xlvt7SFA76MR_PMOx6_VfWc4NcEM_EmcdJIUWMiay5byerdk-qEYt7UlFH69NH9uDpP6RqX01DJiXxWHTPSMCwYP6l-fQkL-AVNYcijXlzwKFi0bACtYP6KbnKIeUIJfHJ-jSIYmJcQkfYD2rqYR_AGkPNIo_c5_tiEcYDoNCqYNvsYpnmEHZpg6iGifKvig699mbUFNGqzBF-iWxfJdFYdWT0mOL-Lp9X3DxffVp_qy6uPn1fvLmvDO77URrSUyYF3Pe6EJbJnxDRNbzsjtBWdpY3sKdXWDkxizKHHgzFWlESLnhDDTqu3B9059xMMpvxA1KOao5t0_KmCdurvincbtQ5b1XZcSNEWgVd3AjHcZEiLmlwyMI7aQ8hJUSoZJlQSUdCX_6DXIUdf1ttTFDesZbJQ9ECZGFKKYO8fQ7DaG64OhqtiuLo1XO1K04vHa9y3_LG3AOwApFLya4gPs_8j-xuEbbqi</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2292053639</pqid></control><display><type>article</type><title>Potent modulation of the CepR quorum sensing receptor and virulence in a Burkholderia cepacia complex member using non-native lactone ligands</title><source>Nature Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Springer Nature OA/Free Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Slinger, Betty L. ; Deay, Jacqueline J. ; Chandler, Josephine R. ; Blackwell, Helen E.</creator><creatorcontrib>Slinger, Betty L. ; Deay, Jacqueline J. ; Chandler, Josephine R. ; Blackwell, Helen E.</creatorcontrib><description>The
Burkholderia cepacia
complex (Bcc) is a family of closely related bacterial pathogens that are the causative agent of deadly human infections. Virulence in Bcc species has been shown to be controlled by the CepI/CepR quorum sensing (QS) system, which is mediated by an
N
-acyl L-homoserine lactone (AHL) signal (C
8
-AHL) and its cognate LuxR-type receptor (CepR). Chemical strategies to block QS in Bcc members would represent an approach to intercept this bacterial communication process and further delineate its role in infection. In the current study, we sought to identify non-native AHLs capable of agonizing or antagonizing CepR, and thereby QS, in a Bcc member. We screened a library of AHL analogs in cell-based reporters for CepR, and identified numerous highly potent CepR agonists and antagonists. These compounds remain active in a Bcc member,
B
.
multivorans
, with one agonist 250-fold more potent than the native ligand C
8
-AHL, and can affect QS-controlled motility. Further, the CepR antagonists prolong
C
.
elegans
survival in an infection model. These AHL analogs are the first reported non-native molecules that both directly modulate CepR and impact QS-controlled phenotypes in a Bcc member, and represent valuable chemical tools to assess the role of QS in Bcc infections.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-49693-x</identifier><identifier>PMID: 31530834</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/95 ; 38/39 ; 38/47 ; 631/326/421 ; 631/92/96 ; 64/11 ; Acyl-Butyrolactones - metabolism ; Agonists ; Animals ; Antibiotics ; Bacteria ; Bacterial Proteins - agonists ; Bacterial Proteins - antagonists & inhibitors ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; beta-Galactosidase - genetics ; Binding sites ; Biofilms ; Burkholderia cepacia ; Burkholderia cepacia complex - drug effects ; Burkholderia cepacia complex - pathogenicity ; Burkholderia Infections - microbiology ; Caenorhabditis elegans - drug effects ; Chemical communication ; Cystic fibrosis ; Drug Evaluation, Preclinical ; Escherichia coli - genetics ; Gene expression ; Genes, Reporter ; Homoserine lactones ; Humanities and Social Sciences ; Infections ; Laboratories ; Ligands ; Motility ; multidisciplinary ; Pathogens ; Phenotypes ; Quorum sensing ; Quorum Sensing - drug effects ; Quorum Sensing - physiology ; Science ; Science (multidisciplinary) ; Virulence</subject><ispartof>Scientific reports, 2019-09, Vol.9 (1), p.13449-12, Article 13449</ispartof><rights>The Author(s) 2019</rights><rights>2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-c86239d47b078f19b31c55bf7c8af87f259b22affd39004eb0dccf8390a8b11c3</citedby><cites>FETCH-LOGICAL-c474t-c86239d47b078f19b31c55bf7c8af87f259b22affd39004eb0dccf8390a8b11c3</cites><orcidid>0000-0003-2235-0522</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748986/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748986/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,27931,27932,41127,42196,51583,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31530834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Slinger, Betty L.</creatorcontrib><creatorcontrib>Deay, Jacqueline J.</creatorcontrib><creatorcontrib>Chandler, Josephine R.</creatorcontrib><creatorcontrib>Blackwell, Helen E.</creatorcontrib><title>Potent modulation of the CepR quorum sensing receptor and virulence in a Burkholderia cepacia complex member using non-native lactone ligands</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The
Burkholderia cepacia
complex (Bcc) is a family of closely related bacterial pathogens that are the causative agent of deadly human infections. Virulence in Bcc species has been shown to be controlled by the CepI/CepR quorum sensing (QS) system, which is mediated by an
N
-acyl L-homoserine lactone (AHL) signal (C
8
-AHL) and its cognate LuxR-type receptor (CepR). Chemical strategies to block QS in Bcc members would represent an approach to intercept this bacterial communication process and further delineate its role in infection. In the current study, we sought to identify non-native AHLs capable of agonizing or antagonizing CepR, and thereby QS, in a Bcc member. We screened a library of AHL analogs in cell-based reporters for CepR, and identified numerous highly potent CepR agonists and antagonists. These compounds remain active in a Bcc member,
B
.
multivorans
, with one agonist 250-fold more potent than the native ligand C
8
-AHL, and can affect QS-controlled motility. Further, the CepR antagonists prolong
C
.
elegans
survival in an infection model. These AHL analogs are the first reported non-native molecules that both directly modulate CepR and impact QS-controlled phenotypes in a Bcc member, and represent valuable chemical tools to assess the role of QS in Bcc infections.</description><subject>13/95</subject><subject>38/39</subject><subject>38/47</subject><subject>631/326/421</subject><subject>631/92/96</subject><subject>64/11</subject><subject>Acyl-Butyrolactones - metabolism</subject><subject>Agonists</subject><subject>Animals</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacterial Proteins - agonists</subject><subject>Bacterial Proteins - antagonists & inhibitors</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>beta-Galactosidase - genetics</subject><subject>Binding sites</subject><subject>Biofilms</subject><subject>Burkholderia cepacia</subject><subject>Burkholderia cepacia complex - drug effects</subject><subject>Burkholderia cepacia complex - pathogenicity</subject><subject>Burkholderia Infections - microbiology</subject><subject>Caenorhabditis elegans - drug effects</subject><subject>Chemical communication</subject><subject>Cystic fibrosis</subject><subject>Drug Evaluation, Preclinical</subject><subject>Escherichia coli - genetics</subject><subject>Gene expression</subject><subject>Genes, Reporter</subject><subject>Homoserine lactones</subject><subject>Humanities and Social Sciences</subject><subject>Infections</subject><subject>Laboratories</subject><subject>Ligands</subject><subject>Motility</subject><subject>multidisciplinary</subject><subject>Pathogens</subject><subject>Phenotypes</subject><subject>Quorum sensing</subject><subject>Quorum Sensing - drug effects</subject><subject>Quorum Sensing - physiology</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Virulence</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1v1DAQhiNERau2f4ADssSFS8BfSewLEqzKh1SpCMHZcpzxrktip3a8Wn4E_xlvt7SFA76MR_PMOx6_VfWc4NcEM_EmcdJIUWMiay5byerdk-qEYt7UlFH69NH9uDpP6RqX01DJiXxWHTPSMCwYP6l-fQkL-AVNYcijXlzwKFi0bACtYP6KbnKIeUIJfHJ-jSIYmJcQkfYD2rqYR_AGkPNIo_c5_tiEcYDoNCqYNvsYpnmEHZpg6iGifKvig699mbUFNGqzBF-iWxfJdFYdWT0mOL-Lp9X3DxffVp_qy6uPn1fvLmvDO77URrSUyYF3Pe6EJbJnxDRNbzsjtBWdpY3sKdXWDkxizKHHgzFWlESLnhDDTqu3B9059xMMpvxA1KOao5t0_KmCdurvincbtQ5b1XZcSNEWgVd3AjHcZEiLmlwyMI7aQ8hJUSoZJlQSUdCX_6DXIUdf1ttTFDesZbJQ9ECZGFKKYO8fQ7DaG64OhqtiuLo1XO1K04vHa9y3_LG3AOwApFLya4gPs_8j-xuEbbqi</recordid><startdate>20190917</startdate><enddate>20190917</enddate><creator>Slinger, Betty L.</creator><creator>Deay, Jacqueline J.</creator><creator>Chandler, Josephine R.</creator><creator>Blackwell, Helen E.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2235-0522</orcidid></search><sort><creationdate>20190917</creationdate><title>Potent modulation of the CepR quorum sensing receptor and virulence in a Burkholderia cepacia complex member using non-native lactone ligands</title><author>Slinger, Betty L. ; Deay, Jacqueline J. ; Chandler, Josephine R. ; Blackwell, Helen E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-c86239d47b078f19b31c55bf7c8af87f259b22affd39004eb0dccf8390a8b11c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>13/95</topic><topic>38/39</topic><topic>38/47</topic><topic>631/326/421</topic><topic>631/92/96</topic><topic>64/11</topic><topic>Acyl-Butyrolactones - metabolism</topic><topic>Agonists</topic><topic>Animals</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacterial Proteins - agonists</topic><topic>Bacterial Proteins - antagonists & inhibitors</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>beta-Galactosidase - genetics</topic><topic>Binding sites</topic><topic>Biofilms</topic><topic>Burkholderia cepacia</topic><topic>Burkholderia cepacia complex - drug effects</topic><topic>Burkholderia cepacia complex - pathogenicity</topic><topic>Burkholderia Infections - microbiology</topic><topic>Caenorhabditis elegans - drug effects</topic><topic>Chemical communication</topic><topic>Cystic fibrosis</topic><topic>Drug Evaluation, Preclinical</topic><topic>Escherichia coli - genetics</topic><topic>Gene expression</topic><topic>Genes, Reporter</topic><topic>Homoserine lactones</topic><topic>Humanities and Social Sciences</topic><topic>Infections</topic><topic>Laboratories</topic><topic>Ligands</topic><topic>Motility</topic><topic>multidisciplinary</topic><topic>Pathogens</topic><topic>Phenotypes</topic><topic>Quorum sensing</topic><topic>Quorum Sensing - drug effects</topic><topic>Quorum Sensing - physiology</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Slinger, Betty L.</creatorcontrib><creatorcontrib>Deay, Jacqueline J.</creatorcontrib><creatorcontrib>Chandler, Josephine R.</creatorcontrib><creatorcontrib>Blackwell, Helen E.</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Slinger, Betty L.</au><au>Deay, Jacqueline J.</au><au>Chandler, Josephine R.</au><au>Blackwell, Helen E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potent modulation of the CepR quorum sensing receptor and virulence in a Burkholderia cepacia complex member using non-native lactone ligands</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-09-17</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>13449</spage><epage>12</epage><pages>13449-12</pages><artnum>13449</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The
Burkholderia cepacia
complex (Bcc) is a family of closely related bacterial pathogens that are the causative agent of deadly human infections. Virulence in Bcc species has been shown to be controlled by the CepI/CepR quorum sensing (QS) system, which is mediated by an
N
-acyl L-homoserine lactone (AHL) signal (C
8
-AHL) and its cognate LuxR-type receptor (CepR). Chemical strategies to block QS in Bcc members would represent an approach to intercept this bacterial communication process and further delineate its role in infection. In the current study, we sought to identify non-native AHLs capable of agonizing or antagonizing CepR, and thereby QS, in a Bcc member. We screened a library of AHL analogs in cell-based reporters for CepR, and identified numerous highly potent CepR agonists and antagonists. These compounds remain active in a Bcc member,
B
.
multivorans
, with one agonist 250-fold more potent than the native ligand C
8
-AHL, and can affect QS-controlled motility. Further, the CepR antagonists prolong
C
.
elegans
survival in an infection model. These AHL analogs are the first reported non-native molecules that both directly modulate CepR and impact QS-controlled phenotypes in a Bcc member, and represent valuable chemical tools to assess the role of QS in Bcc infections.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31530834</pmid><doi>10.1038/s41598-019-49693-x</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-2235-0522</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2045-2322 |
ispartof | Scientific reports, 2019-09, Vol.9 (1), p.13449-12, Article 13449 |
issn | 2045-2322 2045-2322 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6748986 |
source | Nature Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Springer Nature OA/Free Journals; Free Full-Text Journals in Chemistry |
subjects | 13/95 38/39 38/47 631/326/421 631/92/96 64/11 Acyl-Butyrolactones - metabolism Agonists Animals Antibiotics Bacteria Bacterial Proteins - agonists Bacterial Proteins - antagonists & inhibitors Bacterial Proteins - genetics Bacterial Proteins - metabolism beta-Galactosidase - genetics Binding sites Biofilms Burkholderia cepacia Burkholderia cepacia complex - drug effects Burkholderia cepacia complex - pathogenicity Burkholderia Infections - microbiology Caenorhabditis elegans - drug effects Chemical communication Cystic fibrosis Drug Evaluation, Preclinical Escherichia coli - genetics Gene expression Genes, Reporter Homoserine lactones Humanities and Social Sciences Infections Laboratories Ligands Motility multidisciplinary Pathogens Phenotypes Quorum sensing Quorum Sensing - drug effects Quorum Sensing - physiology Science Science (multidisciplinary) Virulence |
title | Potent modulation of the CepR quorum sensing receptor and virulence in a Burkholderia cepacia complex member using non-native lactone ligands |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T04%3A09%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Potent%20modulation%20of%20the%20CepR%20quorum%20sensing%20receptor%20and%20virulence%20in%20a%20Burkholderia%20cepacia%20complex%20member%20using%20non-native%20lactone%20ligands&rft.jtitle=Scientific%20reports&rft.au=Slinger,%20Betty%20L.&rft.date=2019-09-17&rft.volume=9&rft.issue=1&rft.spage=13449&rft.epage=12&rft.pages=13449-12&rft.artnum=13449&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-019-49693-x&rft_dat=%3Cproquest_pubme%3E2292053639%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2292053639&rft_id=info:pmid/31530834&rfr_iscdi=true |