Nuclease dead Cas9 is a programmable roadblock for DNA replication

Limited experimental tools are available to study the consequences of collisions between DNA-bound molecular machines. Here, we repurpose a catalytically inactivated Cas9 (dCas9) construct as a generic, novel, targetable protein–DNA roadblock for studying mechanisms underlying enzymatic activities o...

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Veröffentlicht in:	Scientific reports 2019-09, Vol.9 (1), p.13292-9, Article 13292
Hauptverfasser:	Whinn, Kelsey S., Kaur, Gurleen, Lewis, Jacob S., Schauer, Grant D., Mueller, Stefan H., Jergic, Slobodan, Maynard, Hamish, Gan, Zhong Yan, Naganbabu, Matharishwan, Bruchez, Marcel P., O’Donnell, Michael E., Dixon, Nicholas E., van Oijen, Antoine M., Ghodke, Harshad
Format:	Artikel
Sprache:	eng
Schlagworte:	14/34 45/29 631/337/151/2353 631/337/151/2356 631/45/173 631/57/2265 82/103 CRISPR-Associated Protein 9 - genetics CRISPR-Cas Systems - genetics Deoxyribonucleic acid DNA DNA biosynthesis DNA Replication - genetics DNA, Bacterial - genetics Enzymatic activity Escherichia coli - genetics Humanities and Social Sciences multidisciplinary Nuclease Replication forks RNA, Guide, CRISPR-Cas Systems Science Science (multidisciplinary) Streptococcus pyogenes - enzymology
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