Cytokine gene expression in monocytes of patients undergoing cardiopulmonary bypass surgery evaluated by real‐time PCR

Cardiopulmonary bypass (CPB) surgery induces systemic release of proinflammatory cytokines causing unspecific inflammatory reactions. This study deals with the development of a sensitive technique for detecting changes at the mRNA level in monocytes of patients undergoing CPB surgery, by using real‐...

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Veröffentlicht in:	Journal of cellular and molecular medicine 2003-04, Vol.7 (2), p.146-156
Hauptverfasser:	Zimmermann, Anja K., Simon, P., Seeburger, J., Hoffmann, J., Ziemer, G., Aebert, H., Wendel, H. P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Analysis of Variance cardiopulmonary bypass Cardiopulmonary Bypass - methods Coronary artery cytokines Cytokines - genetics Cytokines - metabolism Gene expression Gene Expression Regulation housekeeping gene Humans Inflammation Inflammation - immunology Inflammation - metabolism Interleukin 8 Mathematics Middle Aged Monocytes Monocytes - physiology Polymerase Chain Reaction real time PCR RNA, Messenger - metabolism RNA-directed DNA polymerase Surgery
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