L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cells
Among cancers, acute lymphoblastic leukemia (ALL) occurs in the children
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| Veröffentlicht in: | Saudi journal of biological sciences 2019-09, Vol.26 (6), p.1146-1153 |
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| container_title | Saudi journal of biological sciences |
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| creator | Fatima, Nuzhath Khan, Mohd. Mazharuddin Khan, Imran Ali |
| description | Among cancers, acute lymphoblastic leukemia (ALL) occurs in the children |
| doi_str_mv | 10.1016/j.sjbs.2019.05.001 |
| format | Article |
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Mazharuddin ; Khan, Imran Ali</creator><creatorcontrib>Fatima, Nuzhath ; Khan, Mohd. Mazharuddin ; Khan, Imran Ali</creatorcontrib><description>Among cancers, acute lymphoblastic leukemia (ALL) occurs in the children <15 years of age. L-asparaginase is an important therapeutic enzyme used for treating ALL. Owing to its therapeutic use and demand, microorganisms have been in use for many years to produce L-asparaginase on an industrial scale. Gram-negative bacteria (Serratia, Erwinia and Escherichia coli) species were used in L-asparaginase. However, earlier studies have documented that the long-term use of enzymes produced from these commercial strains induces hypersensitivity in patients. Therefore, there is a need to discover novel microbial strains producing L-asparaginase with anti-cancer properties, which can be employed for the commercial production of the enzyme. In this study, three strains of Pseudomonas aeruginosa (accession numbers LC425424 (P31), LC425425 (P32), and LC425426 (P34)) isolated from garden soil were screened for the invention of L-asparaginase. Fermented production media was dialyzed to attain the purified enzyme, thus showed a dose-depended cytotoxic effect on HeLa cells, as determined by MTT assay. The IC50s of the different isolates were 86.73, 57.65, and 40.34 µg/mL. These results indicate that pseudomonal L-asparaginase may be used for cancer treatment.</description><identifier>ISSN: 1319-562X</identifier><identifier>EISSN: 2213-7106</identifier><identifier>DOI: 10.1016/j.sjbs.2019.05.001</identifier><identifier>PMID: 31516343</identifier><language>eng</language><publisher>Riyadh, Saudi Arabia: Elsevier B.V</publisher><subject>ALL ; HeLa cells ; L-asparaginase ; Pseudomonas aeruginosa ; الأدوية المضادة للأورام ; الزائفة الزنجارية ; السرطان ; العلاج المناعي</subject><ispartof>Saudi journal of biological sciences, 2019-09, Vol.26 (6), p.1146-1153</ispartof><rights>2019</rights><rights>2019 Production and hosting by Elsevier B.V. on behalf of King Saud University. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-71a71f664cd57f5f050d641aa0e3a3d80614406ed3bb15e39b46f90c700ee3ff3</citedby><cites>FETCH-LOGICAL-c477t-71a71f664cd57f5f050d641aa0e3a3d80614406ed3bb15e39b46f90c700ee3ff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737276/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1319562X19300749$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,3537,27901,27902,53766,53768,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31516343$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fatima, Nuzhath</creatorcontrib><creatorcontrib>Khan, Mohd. Mazharuddin</creatorcontrib><creatorcontrib>Khan, Imran Ali</creatorcontrib><title>L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cells</title><title>Saudi journal of biological sciences</title><addtitle>Saudi J Biol Sci</addtitle><description>Among cancers, acute lymphoblastic leukemia (ALL) occurs in the children <15 years of age. L-asparaginase is an important therapeutic enzyme used for treating ALL. Owing to its therapeutic use and demand, microorganisms have been in use for many years to produce L-asparaginase on an industrial scale. Gram-negative bacteria (Serratia, Erwinia and Escherichia coli) species were used in L-asparaginase. However, earlier studies have documented that the long-term use of enzymes produced from these commercial strains induces hypersensitivity in patients. Therefore, there is a need to discover novel microbial strains producing L-asparaginase with anti-cancer properties, which can be employed for the commercial production of the enzyme. In this study, three strains of Pseudomonas aeruginosa (accession numbers LC425424 (P31), LC425425 (P32), and LC425426 (P34)) isolated from garden soil were screened for the invention of L-asparaginase. Fermented production media was dialyzed to attain the purified enzyme, thus showed a dose-depended cytotoxic effect on HeLa cells, as determined by MTT assay. The IC50s of the different isolates were 86.73, 57.65, and 40.34 µg/mL. These results indicate that pseudomonal L-asparaginase may be used for cancer treatment.</description><subject>ALL</subject><subject>HeLa cells</subject><subject>L-asparaginase</subject><subject>Pseudomonas aeruginosa</subject><subject>الأدوية المضادة للأورام</subject><subject>الزائفة الزنجارية</subject><subject>السرطان</subject><subject>العلاج المناعي</subject><issn>1319-562X</issn><issn>2213-7106</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kV-L1DAUxYso7rj6BQQlj750vGnatAURZNFdYUAfFHwLd9Kb3QxtU3Pbkf32ZpzZQV98CuT-zrl_Tpa9lLCWIPXb3Zp3W14XINs1VGsA-ShbFYVUeS1BP85WUsk2r3Tx4yJ7xrwD0I1q5NPsQslKalWqVTZvcuQJI976EZnEFEO3WOqEi2EQHHwvPIceZ2IRnPjKtHRhCIkVSHFJqsAo-C78YjGFmcZZ4Dj73OJoKQq0s9_7-V6EUdzQBoWlvufn2ROHPdOL03uZff_08dvVTb75cv356sMmt2Vdz2kLrKXTurRdVbvKQQWdLiUikELVNaBlWYKmTm23siLVbkvtWrA1AJFyTl1m74--07IdqLNpuoi9maIfMN6bgN78Wxn9nbkNe6NrVRe1TgZvTgYx_FyIZzN4PqyAI4WFTVG00OgipZHQ4ojaGJgjuXMbCeYQl9mZQ1zmEJeBysAf0eu_BzxLHvJJwKsjQOmfHJ6Jpmll06T6u1M9nXHvKRq2ntLpOx_JzqYL_n_9fwMswbOz</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Fatima, Nuzhath</creator><creator>Khan, Mohd. Mazharuddin</creator><creator>Khan, Imran Ali</creator><general>Elsevier B.V</general><general>Saudi Biological Society</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>ADJCN</scope><scope>AHFXO</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190901</creationdate><title>L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cells</title><author>Fatima, Nuzhath ; Khan, Mohd. Mazharuddin ; Khan, Imran Ali</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-71a71f664cd57f5f050d641aa0e3a3d80614406ed3bb15e39b46f90c700ee3ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>ALL</topic><topic>HeLa cells</topic><topic>L-asparaginase</topic><topic>Pseudomonas aeruginosa</topic><topic>الأدوية المضادة للأورام</topic><topic>الزائفة الزنجارية</topic><topic>السرطان</topic><topic>العلاج المناعي</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fatima, Nuzhath</creatorcontrib><creatorcontrib>Khan, Mohd. Mazharuddin</creatorcontrib><creatorcontrib>Khan, Imran Ali</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Saudi journal of biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fatima, Nuzhath</au><au>Khan, Mohd. Mazharuddin</au><au>Khan, Imran Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cells</atitle><jtitle>Saudi journal of biological sciences</jtitle><addtitle>Saudi J Biol Sci</addtitle><date>2019-09-01</date><risdate>2019</risdate><volume>26</volume><issue>6</issue><spage>1146</spage><epage>1153</epage><pages>1146-1153</pages><issn>1319-562X</issn><eissn>2213-7106</eissn><abstract>Among cancers, acute lymphoblastic leukemia (ALL) occurs in the children <15 years of age. L-asparaginase is an important therapeutic enzyme used for treating ALL. Owing to its therapeutic use and demand, microorganisms have been in use for many years to produce L-asparaginase on an industrial scale. Gram-negative bacteria (Serratia, Erwinia and Escherichia coli) species were used in L-asparaginase. However, earlier studies have documented that the long-term use of enzymes produced from these commercial strains induces hypersensitivity in patients. Therefore, there is a need to discover novel microbial strains producing L-asparaginase with anti-cancer properties, which can be employed for the commercial production of the enzyme. In this study, three strains of Pseudomonas aeruginosa (accession numbers LC425424 (P31), LC425425 (P32), and LC425426 (P34)) isolated from garden soil were screened for the invention of L-asparaginase. Fermented production media was dialyzed to attain the purified enzyme, thus showed a dose-depended cytotoxic effect on HeLa cells, as determined by MTT assay. The IC50s of the different isolates were 86.73, 57.65, and 40.34 µg/mL. 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| subjects | ALL HeLa cells L-asparaginase Pseudomonas aeruginosa الأدوية المضادة للأورام الزائفة الزنجارية السرطان العلاج المناعي |
| title | L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cells |
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