The Conserved IgSF9 Protein Borderless Regulates Axonal Transport of Presynaptic Components and Color Vision in Drosophila
Normal brain function requires proper targeting of synaptic-vesicle (SV) and active-zone components for presynaptic assembly and function. Whether and how synaptogenic signals (e.g., adhesion) at axo-dendritic contact sites promote axonal transport of presynaptic components for synapse formation, ho...
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creator | Shaw, Hunter S Cameron, Scott A Chang, Wen-Tzu Rao, Yong |
description | Normal brain function requires proper targeting of synaptic-vesicle (SV) and active-zone components for presynaptic assembly and function. Whether and how synaptogenic signals (e.g., adhesion) at axo-dendritic contact sites promote axonal transport of presynaptic components for synapse formation, however, remain unclear. In this study, we show that Borderless (Bdl), a member of the conserved IgSF9-family trans-synaptic cell adhesion molecules, plays a novel and specific role in regulating axonal transport of SV components. Loss of
disrupts axonal transport of SV components in photoreceptor R8 axons, but does not affect the transport of mitochondria. Genetic mosaic analysis, transgene rescue and cell-type-specific knockdown indicate that Bdl is required both presynaptically and postsynaptically for delivering SV components in R8 axons. Consistent with a role for Bdl in R8 axons, loss of
causes a failure of R8-dependent phototaxis response to green light.
interacts genetically with
encoding for a member of the UNC-104/Imac/KIF1A-family motor proteins, and is required for proper localization of Imac in R8 presynaptic terminals. Our results support a model in which Bdl mediates specific axo-dendritic interactions in a homophilic manner, which upregulates the Imac motor in promoting axonal transport of SV components for R8 presynaptic assembly and function.
Whether and how synaptogenic adhesion at axo-dendritic contact sites regulates axonal transport of presynaptic components remain unknown. Here we show for the first time that a trans-synaptic adhesion molecule mediates specific interactions at axo-dendritic contact sites, which is required for upregulating the UNC-104/Imac/KIF1A motor in promoting axonal transport of synaptic-vesicle components for presynaptic assembly and function. |
doi_str_mv | 10.1523/JNEUROSCI.0075-19.2019 |
format | Article |
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disrupts axonal transport of SV components in photoreceptor R8 axons, but does not affect the transport of mitochondria. Genetic mosaic analysis, transgene rescue and cell-type-specific knockdown indicate that Bdl is required both presynaptically and postsynaptically for delivering SV components in R8 axons. Consistent with a role for Bdl in R8 axons, loss of
causes a failure of R8-dependent phototaxis response to green light.
interacts genetically with
encoding for a member of the UNC-104/Imac/KIF1A-family motor proteins, and is required for proper localization of Imac in R8 presynaptic terminals. Our results support a model in which Bdl mediates specific axo-dendritic interactions in a homophilic manner, which upregulates the Imac motor in promoting axonal transport of SV components for R8 presynaptic assembly and function.
Whether and how synaptogenic adhesion at axo-dendritic contact sites regulates axonal transport of presynaptic components remain unknown. Here we show for the first time that a trans-synaptic adhesion molecule mediates specific interactions at axo-dendritic contact sites, which is required for upregulating the UNC-104/Imac/KIF1A motor in promoting axonal transport of synaptic-vesicle components for presynaptic assembly and function.</description><identifier>ISSN: 0270-6474</identifier><identifier>EISSN: 1529-2401</identifier><identifier>DOI: 10.1523/JNEUROSCI.0075-19.2019</identifier><identifier>PMID: 31235647</identifier><language>eng</language><publisher>United States: Society for Neuroscience</publisher><subject>Adhesion ; Assembly ; Axonal transport ; Axons ; Cell adhesion ; Cell adhesion & migration ; Cell adhesion molecules ; Color vision ; Drosophila ; Fruit flies ; Genetic analysis ; Light effects ; Localization ; Mitochondria ; Phototaxis ; Protein transport ; Proteins ; Synaptogenesis ; Transport buildings, stations and terminals</subject><ispartof>The Journal of neuroscience, 2019-08, Vol.39 (35), p.6817-6828</ispartof><rights>Copyright © 2019 the authors.</rights><rights>Copyright Society for Neuroscience Aug 28, 2019</rights><rights>Copyright © 2019 the authors 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-9bffc986f909edd627b4f3d14fb26acfbf4b97a242d098734046f5e024aaaafc3</citedby><orcidid>0000-0003-2137-5971</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733566/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733566/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31235647$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shaw, Hunter S</creatorcontrib><creatorcontrib>Cameron, Scott A</creatorcontrib><creatorcontrib>Chang, Wen-Tzu</creatorcontrib><creatorcontrib>Rao, Yong</creatorcontrib><title>The Conserved IgSF9 Protein Borderless Regulates Axonal Transport of Presynaptic Components and Color Vision in Drosophila</title><title>The Journal of neuroscience</title><addtitle>J Neurosci</addtitle><description>Normal brain function requires proper targeting of synaptic-vesicle (SV) and active-zone components for presynaptic assembly and function. Whether and how synaptogenic signals (e.g., adhesion) at axo-dendritic contact sites promote axonal transport of presynaptic components for synapse formation, however, remain unclear. In this study, we show that Borderless (Bdl), a member of the conserved IgSF9-family trans-synaptic cell adhesion molecules, plays a novel and specific role in regulating axonal transport of SV components. Loss of
disrupts axonal transport of SV components in photoreceptor R8 axons, but does not affect the transport of mitochondria. Genetic mosaic analysis, transgene rescue and cell-type-specific knockdown indicate that Bdl is required both presynaptically and postsynaptically for delivering SV components in R8 axons. Consistent with a role for Bdl in R8 axons, loss of
causes a failure of R8-dependent phototaxis response to green light.
interacts genetically with
encoding for a member of the UNC-104/Imac/KIF1A-family motor proteins, and is required for proper localization of Imac in R8 presynaptic terminals. Our results support a model in which Bdl mediates specific axo-dendritic interactions in a homophilic manner, which upregulates the Imac motor in promoting axonal transport of SV components for R8 presynaptic assembly and function.
Whether and how synaptogenic adhesion at axo-dendritic contact sites regulates axonal transport of presynaptic components remain unknown. Here we show for the first time that a trans-synaptic adhesion molecule mediates specific interactions at axo-dendritic contact sites, which is required for upregulating the UNC-104/Imac/KIF1A motor in promoting axonal transport of synaptic-vesicle components for presynaptic assembly and function.</description><subject>Adhesion</subject><subject>Assembly</subject><subject>Axonal transport</subject><subject>Axons</subject><subject>Cell adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell adhesion molecules</subject><subject>Color vision</subject><subject>Drosophila</subject><subject>Fruit flies</subject><subject>Genetic analysis</subject><subject>Light effects</subject><subject>Localization</subject><subject>Mitochondria</subject><subject>Phototaxis</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>Synaptogenesis</subject><subject>Transport buildings, stations and terminals</subject><issn>0270-6474</issn><issn>1529-2401</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpdkc1uEzEUhS0EoqHwCpUlNmwm-G888QaphBaCKoralK3lmblOXDn21J6pKE-Po5YI8MayznePrs9B6ISSOa0Zf__129nN1eX1cjUnpKkrquaMUPUMzYqqKiYIfY5mhDWkkqIRR-hVzrekoIQ2L9ERp4zXRZihX-st4GUMGdI99Hi1uT5X-HuKI7iAP8bUQ_KQM76CzeTNCBmf_ozBeLxOJuQhphFHWwYgPwQzjK4rZrshBghjxib05eljwj9cdjHg4vkpxRyHrfPmNXphjc_w5uk-RjfnZ-vll-ri8vNqeXpRdUKwsVKttZ1aSKuIgr6XrGmF5T0VtmXSdLa1olWNYYL1RC0aLoiQtgbChCnHdvwYfXj0HaZ2B31XVkvG6yG5nUkPOhqn_1WC2-pNvNey4SUlWQzePRmkeDdBHvXO5Q68NwHilDVjQqoSraQFffsfehunVPLaU4uaNJILVSj5SHUljJzAHpahRO_r1Yd69b5eTZXe11sGT_7-ymHsT5_8N-FapMg</recordid><startdate>20190828</startdate><enddate>20190828</enddate><creator>Shaw, Hunter S</creator><creator>Cameron, Scott A</creator><creator>Chang, Wen-Tzu</creator><creator>Rao, Yong</creator><general>Society for Neuroscience</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2137-5971</orcidid></search><sort><creationdate>20190828</creationdate><title>The Conserved IgSF9 Protein Borderless Regulates Axonal Transport of Presynaptic Components and Color Vision in Drosophila</title><author>Shaw, Hunter S ; Cameron, Scott A ; Chang, Wen-Tzu ; Rao, Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-9bffc986f909edd627b4f3d14fb26acfbf4b97a242d098734046f5e024aaaafc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adhesion</topic><topic>Assembly</topic><topic>Axonal transport</topic><topic>Axons</topic><topic>Cell adhesion</topic><topic>Cell adhesion & migration</topic><topic>Cell adhesion molecules</topic><topic>Color vision</topic><topic>Drosophila</topic><topic>Fruit flies</topic><topic>Genetic analysis</topic><topic>Light effects</topic><topic>Localization</topic><topic>Mitochondria</topic><topic>Phototaxis</topic><topic>Protein transport</topic><topic>Proteins</topic><topic>Synaptogenesis</topic><topic>Transport buildings, stations and terminals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shaw, Hunter S</creatorcontrib><creatorcontrib>Cameron, Scott A</creatorcontrib><creatorcontrib>Chang, Wen-Tzu</creatorcontrib><creatorcontrib>Rao, Yong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shaw, Hunter S</au><au>Cameron, Scott A</au><au>Chang, Wen-Tzu</au><au>Rao, Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Conserved IgSF9 Protein Borderless Regulates Axonal Transport of Presynaptic Components and Color Vision in Drosophila</atitle><jtitle>The Journal of neuroscience</jtitle><addtitle>J Neurosci</addtitle><date>2019-08-28</date><risdate>2019</risdate><volume>39</volume><issue>35</issue><spage>6817</spage><epage>6828</epage><pages>6817-6828</pages><issn>0270-6474</issn><eissn>1529-2401</eissn><abstract>Normal brain function requires proper targeting of synaptic-vesicle (SV) and active-zone components for presynaptic assembly and function. Whether and how synaptogenic signals (e.g., adhesion) at axo-dendritic contact sites promote axonal transport of presynaptic components for synapse formation, however, remain unclear. In this study, we show that Borderless (Bdl), a member of the conserved IgSF9-family trans-synaptic cell adhesion molecules, plays a novel and specific role in regulating axonal transport of SV components. Loss of
disrupts axonal transport of SV components in photoreceptor R8 axons, but does not affect the transport of mitochondria. Genetic mosaic analysis, transgene rescue and cell-type-specific knockdown indicate that Bdl is required both presynaptically and postsynaptically for delivering SV components in R8 axons. Consistent with a role for Bdl in R8 axons, loss of
causes a failure of R8-dependent phototaxis response to green light.
interacts genetically with
encoding for a member of the UNC-104/Imac/KIF1A-family motor proteins, and is required for proper localization of Imac in R8 presynaptic terminals. Our results support a model in which Bdl mediates specific axo-dendritic interactions in a homophilic manner, which upregulates the Imac motor in promoting axonal transport of SV components for R8 presynaptic assembly and function.
Whether and how synaptogenic adhesion at axo-dendritic contact sites regulates axonal transport of presynaptic components remain unknown. Here we show for the first time that a trans-synaptic adhesion molecule mediates specific interactions at axo-dendritic contact sites, which is required for upregulating the UNC-104/Imac/KIF1A motor in promoting axonal transport of synaptic-vesicle components for presynaptic assembly and function.</abstract><cop>United States</cop><pub>Society for Neuroscience</pub><pmid>31235647</pmid><doi>10.1523/JNEUROSCI.0075-19.2019</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-2137-5971</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Adhesion Assembly Axonal transport Axons Cell adhesion Cell adhesion & migration Cell adhesion molecules Color vision Drosophila Fruit flies Genetic analysis Light effects Localization Mitochondria Phototaxis Protein transport Proteins Synaptogenesis Transport buildings, stations and terminals |
title | The Conserved IgSF9 Protein Borderless Regulates Axonal Transport of Presynaptic Components and Color Vision in Drosophila |
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