$Asymmetric-flow field-flow fractionation technology for exomere and small extracellular vesicle separation and characterization$

Asymmetric-flow field-flow fractionation technology for exomere and small extracellular vesicle separation and characterization

We describe the protocol development and optimization of asymmetric-flow field-flow fractionation (AF4) technology for separating and characterizing extracellular nanoparticles (ENPs), particularly small extracellular vesicles (sEVs), known as exosomes, and even smaller novel nanoparticles, known as...

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Veröffentlicht in:	Nature protocols 2019-04, Vol.14 (4), p.1027-1053
Hauptverfasser:	Zhang, Haiying, Lyden, David
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2196 631/1647/2230 631/57/2282 631/61/350/877 Analytical Chemistry Animals Atomic properties Biological Techniques Biomedical and Life Sciences Cell culture Cell fractionation Cell separation Cell-Derived Microparticles - chemistry Channel flow Composition Computational Biology/Bioinformatics Conditioning Cross flow Culture Media, Conditioned - chemistry Exosomes Exosomes - chemistry Fractionation Fractionation, Field Flow - instrumentation Fractionation, Field Flow - methods Humans Hydrodynamics Life Sciences Light scattering Melanoma, Experimental - chemistry Melanoma, Experimental - pathology Membranes, Artificial Methods Mice Microarrays Micrometers Molecular biology Nanoparticles Nanoparticles - chemistry Nanoparticles - ultrastructure Optimization Organelles Organic Chemistry Particle Size Photon correlation spectroscopy Protocol Separation Tumor Cells, Cultured Ultracentrifugation Ultraviolet radiation
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