Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues

Spatial mapping of proteins in tissues is hindered by limitations in multiplexing, sensitivity and throughput. Here we report immunostaining with signal amplification by exchange reaction (Immuno-SABER), which achieves highly multiplexed signal amplification via DNA-barcoded antibodies and orthogona...

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Veröffentlicht in:	Nature biotechnology 2019-09, Vol.37 (9), p.1080-1090
Hauptverfasser:	Saka, Sinem K., Wang, Yu, Kishi, Jocelyn Y., Zhu, Allen, Zeng, Yitian, Xie, Wenxin, Kirli, Koray, Yapp, Clarence, Cicconet, Marcelo, Beliveau, Brian J., Lapan, Sylvain W., Yin, Siyuan, Lin, Millicent, Boyden, Edward S., Kaeser, Pascal S., Pihan, German, Church, George M., Yin, Peng
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2163 631/1647/245/2226 631/1647/664/1257 631/80/2373/2238 639/624/399/54/992 Agriculture Amplification Animals Antibodies Antibodies - immunology Antibodies - metabolism Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Cell Line Concatamers Deoxyribonucleic acid Diagnostic imaging DNA DNA - analysis DNA Barcoding, Taxonomic Exchanging Fluorescence Fluorescent Dyes Humans Image resolution Immunohistochemistry - methods In Situ Hybridization, Fluorescence - methods Life Sciences Methods Mice Microscopy, Fluorescence - methods Multiplexing Paraffin Paraffins Peptide mapping Proteins Proteins - metabolism Retina - cytology Sensitivity Staining and Labeling
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