Clinical Evaluation of a Multiplex PCR for the Detection of Salmonella enterica Serovars Typhi and Paratyphi A from Blood Specimens in a High-Endemic Setting
Enteric fever is a major public health concern in endemic areas, particularly in infrastructure-limited countries where Paratyphi A has emerged in increasing proportion of cases. We aimed to evaluate a method to detect Typhi ( Typhi) and Paratyphi A ( Paratyphi A) in febrile patients in Bangladesh....
Gespeichert in:
Veröffentlicht in: | The American journal of tropical medicine and hygiene 2019-01, Vol.101 (3), p.513-520 |
---|---|
Hauptverfasser: | , , , , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 520 |
---|---|
container_issue | 3 |
container_start_page | 513 |
container_title | The American journal of tropical medicine and hygiene |
container_volume | 101 |
creator | Pouzol, Stephane Tanmoy, Arif Mohammad Ahmed, Dilruba Khanam, Farhana Brooks, W Abdullah Bhuyan, Golam Sarower Fabre, Laetitia Bryant, Juliet E Gustin, Marie-Paule Vanhems, Philippe Carman, Bill Weill, François-Xavier Qadri, Firdausi Saha, Samir Endtz, Hubert |
description | Enteric fever is a major public health concern in endemic areas, particularly in infrastructure-limited countries where
Paratyphi A has emerged in increasing proportion of cases. We aimed to evaluate a method to detect
Typhi (
Typhi) and
Paratyphi A (
Paratyphi A) in febrile patients in Bangladesh. We conducted a prospective study enrolling patients with fever > 38°C admitted to two large urban hospitals and two outpatient clinics located in Dhaka, Bangladesh. We developed and evaluated a method combining short culture with a new molecular assay to simultaneously detect and differentiate
Typhi and
Paratyphi A from other
directly from 2 to 4 mL of whole blood in febrile patients (
= 680). A total of 680 cases were enrolled from the four participating sites. An increase in the detection rate (+38.8%) in
Typhi and
. Paratyphi A was observed with a multiplex polymerase chain reaction (PCR) assay, and absence of non-typhoidal
detection was reported. All 45 healthy controls were culture and PCR negative, generating an estimated 92.9% of specificity on clinical samples. When clinical performance was assessed in the absence of blood volume prioritization for testing, a latent class model estimates clinical performance ≥ 95% in sensitivity and specificity with likelihood ratio (LR) LR+ > 10 and LR- < 0.1 for the multiplex PCR assay. The alternative method to blood culture we developed may be useful alone or in combination with culture or serological tests for epidemiological studies in high disease burden settings and should be considered as secondary endpoint test for future vaccine trials. |
doi_str_mv | 10.4269/ajtmh.18-0992 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6726943</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2306475935</sourcerecordid><originalsourceid>FETCH-LOGICAL-c453t-6df3f6ac27b2901b832260f287fc25d0812180ed42b05532ed9ff1ca169650253</originalsourceid><addsrcrecordid>eNpdkktvEzEUhUcIRNPCki2yxIbNFD_GnpkNUgiBIAVRkbK2HI-dceSxp7Ynoj-m_xWnL6Ary_Lnc--59xTFGwTPK8zaD2Kfhv4cNSVsW_ysmKGqZiViFX1ezCCEuGwZqU-K0xj3EKIGI_SyOCEINzWsmllxs7DGGSksWB6EnUQy3gGvgQDfJ5vMaNVvcLH4CbQPIPUKfFZJyQdoI-zgnbJWAOWSClkHbFTwBxEiuLweewOE68CFCCLd3uZABz-AT9b7DmxGJc2gXATG5Xors-vLpevUYGRWScm43avihRY2qtf351nx68vycrEq1z--flvM16WsKEkl6zTRTEhcb3EL0bYhGDOos0ctMe1ggzBqoOoqvIWUEqy6VmskBWItoxBTclZ8vNMdp-2gOpndBGH5GMwgwjX3wvD_X5zp-c4fOKvzDiqSBco7gf7Jt9V8zUcRk5oCh7hqMG3oAWX-_X3B4K8mFRMfTJTHSTrlp8gxphVFLWFNRt89Qfd-Ci6Pg2MCWVXTltC_DcjgYwxKP3aBID8Ghd8GhaOGH4OS-bf_On6kH5JB_gDskLrE</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2306475935</pqid></control><display><type>article</type><title>Clinical Evaluation of a Multiplex PCR for the Detection of Salmonella enterica Serovars Typhi and Paratyphi A from Blood Specimens in a High-Endemic Setting</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Pouzol, Stephane ; Tanmoy, Arif Mohammad ; Ahmed, Dilruba ; Khanam, Farhana ; Brooks, W Abdullah ; Bhuyan, Golam Sarower ; Fabre, Laetitia ; Bryant, Juliet E ; Gustin, Marie-Paule ; Vanhems, Philippe ; Carman, Bill ; Weill, François-Xavier ; Qadri, Firdausi ; Saha, Samir ; Endtz, Hubert</creator><creatorcontrib>Pouzol, Stephane ; Tanmoy, Arif Mohammad ; Ahmed, Dilruba ; Khanam, Farhana ; Brooks, W Abdullah ; Bhuyan, Golam Sarower ; Fabre, Laetitia ; Bryant, Juliet E ; Gustin, Marie-Paule ; Vanhems, Philippe ; Carman, Bill ; Weill, François-Xavier ; Qadri, Firdausi ; Saha, Samir ; Endtz, Hubert</creatorcontrib><description>Enteric fever is a major public health concern in endemic areas, particularly in infrastructure-limited countries where
Paratyphi A has emerged in increasing proportion of cases. We aimed to evaluate a method to detect
Typhi (
Typhi) and
Paratyphi A (
Paratyphi A) in febrile patients in Bangladesh. We conducted a prospective study enrolling patients with fever > 38°C admitted to two large urban hospitals and two outpatient clinics located in Dhaka, Bangladesh. We developed and evaluated a method combining short culture with a new molecular assay to simultaneously detect and differentiate
Typhi and
Paratyphi A from other
directly from 2 to 4 mL of whole blood in febrile patients (
= 680). A total of 680 cases were enrolled from the four participating sites. An increase in the detection rate (+38.8%) in
Typhi and
. Paratyphi A was observed with a multiplex polymerase chain reaction (PCR) assay, and absence of non-typhoidal
detection was reported. All 45 healthy controls were culture and PCR negative, generating an estimated 92.9% of specificity on clinical samples. When clinical performance was assessed in the absence of blood volume prioritization for testing, a latent class model estimates clinical performance ≥ 95% in sensitivity and specificity with likelihood ratio (LR) LR+ > 10 and LR- < 0.1 for the multiplex PCR assay. The alternative method to blood culture we developed may be useful alone or in combination with culture or serological tests for epidemiological studies in high disease burden settings and should be considered as secondary endpoint test for future vaccine trials.</description><identifier>ISSN: 0002-9637</identifier><identifier>EISSN: 1476-1645</identifier><identifier>DOI: 10.4269/ajtmh.18-0992</identifier><identifier>PMID: 31287048</identifier><language>eng</language><publisher>United States: Institute of Tropical Medicine</publisher><subject>Adolescent ; Adult ; Bacteriology ; Bangladesh ; Blood ; Child ; Child, Preschool ; Endemic Diseases ; Humans ; Life Sciences ; Microbiology and Parasitology ; Multiplex Polymerase Chain Reaction - standards ; Prospective Studies ; Salmonella ; Salmonella paratyphi A - immunology ; Salmonella paratyphi A - isolation & purification ; Salmonella typhi - immunology ; Salmonella typhi - isolation & purification ; Sensitivity and Specificity ; Serogroup ; Typhoid Fever - blood ; Typhoid Fever - diagnosis ; Typhoid Fever - microbiology ; Vaccines</subject><ispartof>The American journal of tropical medicine and hygiene, 2019-01, Vol.101 (3), p.513-520</ispartof><rights>Copyright Institute of Tropical Medicine 2019</rights><rights>Attribution</rights><rights>The American Society of Tropical Medicine and Hygiene 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-6df3f6ac27b2901b832260f287fc25d0812180ed42b05532ed9ff1ca169650253</citedby><orcidid>0000-0001-9941-5799 ; 0009-0003-9048-663X ; 0000-0003-3188-1456</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726943/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726943/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31287048$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://pasteur.hal.science/pasteur-02482585$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Pouzol, Stephane</creatorcontrib><creatorcontrib>Tanmoy, Arif Mohammad</creatorcontrib><creatorcontrib>Ahmed, Dilruba</creatorcontrib><creatorcontrib>Khanam, Farhana</creatorcontrib><creatorcontrib>Brooks, W Abdullah</creatorcontrib><creatorcontrib>Bhuyan, Golam Sarower</creatorcontrib><creatorcontrib>Fabre, Laetitia</creatorcontrib><creatorcontrib>Bryant, Juliet E</creatorcontrib><creatorcontrib>Gustin, Marie-Paule</creatorcontrib><creatorcontrib>Vanhems, Philippe</creatorcontrib><creatorcontrib>Carman, Bill</creatorcontrib><creatorcontrib>Weill, François-Xavier</creatorcontrib><creatorcontrib>Qadri, Firdausi</creatorcontrib><creatorcontrib>Saha, Samir</creatorcontrib><creatorcontrib>Endtz, Hubert</creatorcontrib><title>Clinical Evaluation of a Multiplex PCR for the Detection of Salmonella enterica Serovars Typhi and Paratyphi A from Blood Specimens in a High-Endemic Setting</title><title>The American journal of tropical medicine and hygiene</title><addtitle>Am J Trop Med Hyg</addtitle><description>Enteric fever is a major public health concern in endemic areas, particularly in infrastructure-limited countries where
Paratyphi A has emerged in increasing proportion of cases. We aimed to evaluate a method to detect
Typhi (
Typhi) and
Paratyphi A (
Paratyphi A) in febrile patients in Bangladesh. We conducted a prospective study enrolling patients with fever > 38°C admitted to two large urban hospitals and two outpatient clinics located in Dhaka, Bangladesh. We developed and evaluated a method combining short culture with a new molecular assay to simultaneously detect and differentiate
Typhi and
Paratyphi A from other
directly from 2 to 4 mL of whole blood in febrile patients (
= 680). A total of 680 cases were enrolled from the four participating sites. An increase in the detection rate (+38.8%) in
Typhi and
. Paratyphi A was observed with a multiplex polymerase chain reaction (PCR) assay, and absence of non-typhoidal
detection was reported. All 45 healthy controls were culture and PCR negative, generating an estimated 92.9% of specificity on clinical samples. When clinical performance was assessed in the absence of blood volume prioritization for testing, a latent class model estimates clinical performance ≥ 95% in sensitivity and specificity with likelihood ratio (LR) LR+ > 10 and LR- < 0.1 for the multiplex PCR assay. The alternative method to blood culture we developed may be useful alone or in combination with culture or serological tests for epidemiological studies in high disease burden settings and should be considered as secondary endpoint test for future vaccine trials.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Bacteriology</subject><subject>Bangladesh</subject><subject>Blood</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Endemic Diseases</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Microbiology and Parasitology</subject><subject>Multiplex Polymerase Chain Reaction - standards</subject><subject>Prospective Studies</subject><subject>Salmonella</subject><subject>Salmonella paratyphi A - immunology</subject><subject>Salmonella paratyphi A - isolation & purification</subject><subject>Salmonella typhi - immunology</subject><subject>Salmonella typhi - isolation & purification</subject><subject>Sensitivity and Specificity</subject><subject>Serogroup</subject><subject>Typhoid Fever - blood</subject><subject>Typhoid Fever - diagnosis</subject><subject>Typhoid Fever - microbiology</subject><subject>Vaccines</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkktvEzEUhUcIRNPCki2yxIbNFD_GnpkNUgiBIAVRkbK2HI-dceSxp7Ynoj-m_xWnL6Ary_Lnc--59xTFGwTPK8zaD2Kfhv4cNSVsW_ysmKGqZiViFX1ezCCEuGwZqU-K0xj3EKIGI_SyOCEINzWsmllxs7DGGSksWB6EnUQy3gGvgQDfJ5vMaNVvcLH4CbQPIPUKfFZJyQdoI-zgnbJWAOWSClkHbFTwBxEiuLweewOE68CFCCLd3uZABz-AT9b7DmxGJc2gXATG5Xors-vLpevUYGRWScm43avihRY2qtf351nx68vycrEq1z--flvM16WsKEkl6zTRTEhcb3EL0bYhGDOos0ctMe1ggzBqoOoqvIWUEqy6VmskBWItoxBTclZ8vNMdp-2gOpndBGH5GMwgwjX3wvD_X5zp-c4fOKvzDiqSBco7gf7Jt9V8zUcRk5oCh7hqMG3oAWX-_X3B4K8mFRMfTJTHSTrlp8gxphVFLWFNRt89Qfd-Ci6Pg2MCWVXTltC_DcjgYwxKP3aBID8Ghd8GhaOGH4OS-bf_On6kH5JB_gDskLrE</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Pouzol, Stephane</creator><creator>Tanmoy, Arif Mohammad</creator><creator>Ahmed, Dilruba</creator><creator>Khanam, Farhana</creator><creator>Brooks, W Abdullah</creator><creator>Bhuyan, Golam Sarower</creator><creator>Fabre, Laetitia</creator><creator>Bryant, Juliet E</creator><creator>Gustin, Marie-Paule</creator><creator>Vanhems, Philippe</creator><creator>Carman, Bill</creator><creator>Weill, François-Xavier</creator><creator>Qadri, Firdausi</creator><creator>Saha, Samir</creator><creator>Endtz, Hubert</creator><general>Institute of Tropical Medicine</general><general>American Society of Tropical Medicine and Hygiene</general><general>The American Society of Tropical Medicine and Hygiene</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9941-5799</orcidid><orcidid>https://orcid.org/0009-0003-9048-663X</orcidid><orcidid>https://orcid.org/0000-0003-3188-1456</orcidid></search><sort><creationdate>20190101</creationdate><title>Clinical Evaluation of a Multiplex PCR for the Detection of Salmonella enterica Serovars Typhi and Paratyphi A from Blood Specimens in a High-Endemic Setting</title><author>Pouzol, Stephane ; Tanmoy, Arif Mohammad ; Ahmed, Dilruba ; Khanam, Farhana ; Brooks, W Abdullah ; Bhuyan, Golam Sarower ; Fabre, Laetitia ; Bryant, Juliet E ; Gustin, Marie-Paule ; Vanhems, Philippe ; Carman, Bill ; Weill, François-Xavier ; Qadri, Firdausi ; Saha, Samir ; Endtz, Hubert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-6df3f6ac27b2901b832260f287fc25d0812180ed42b05532ed9ff1ca169650253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Bacteriology</topic><topic>Bangladesh</topic><topic>Blood</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Endemic Diseases</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Microbiology and Parasitology</topic><topic>Multiplex Polymerase Chain Reaction - standards</topic><topic>Prospective Studies</topic><topic>Salmonella</topic><topic>Salmonella paratyphi A - immunology</topic><topic>Salmonella paratyphi A - isolation & purification</topic><topic>Salmonella typhi - immunology</topic><topic>Salmonella typhi - isolation & purification</topic><topic>Sensitivity and Specificity</topic><topic>Serogroup</topic><topic>Typhoid Fever - blood</topic><topic>Typhoid Fever - diagnosis</topic><topic>Typhoid Fever - microbiology</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pouzol, Stephane</creatorcontrib><creatorcontrib>Tanmoy, Arif Mohammad</creatorcontrib><creatorcontrib>Ahmed, Dilruba</creatorcontrib><creatorcontrib>Khanam, Farhana</creatorcontrib><creatorcontrib>Brooks, W Abdullah</creatorcontrib><creatorcontrib>Bhuyan, Golam Sarower</creatorcontrib><creatorcontrib>Fabre, Laetitia</creatorcontrib><creatorcontrib>Bryant, Juliet E</creatorcontrib><creatorcontrib>Gustin, Marie-Paule</creatorcontrib><creatorcontrib>Vanhems, Philippe</creatorcontrib><creatorcontrib>Carman, Bill</creatorcontrib><creatorcontrib>Weill, François-Xavier</creatorcontrib><creatorcontrib>Qadri, Firdausi</creatorcontrib><creatorcontrib>Saha, Samir</creatorcontrib><creatorcontrib>Endtz, Hubert</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The American journal of tropical medicine and hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pouzol, Stephane</au><au>Tanmoy, Arif Mohammad</au><au>Ahmed, Dilruba</au><au>Khanam, Farhana</au><au>Brooks, W Abdullah</au><au>Bhuyan, Golam Sarower</au><au>Fabre, Laetitia</au><au>Bryant, Juliet E</au><au>Gustin, Marie-Paule</au><au>Vanhems, Philippe</au><au>Carman, Bill</au><au>Weill, François-Xavier</au><au>Qadri, Firdausi</au><au>Saha, Samir</au><au>Endtz, Hubert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical Evaluation of a Multiplex PCR for the Detection of Salmonella enterica Serovars Typhi and Paratyphi A from Blood Specimens in a High-Endemic Setting</atitle><jtitle>The American journal of tropical medicine and hygiene</jtitle><addtitle>Am J Trop Med Hyg</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>101</volume><issue>3</issue><spage>513</spage><epage>520</epage><pages>513-520</pages><issn>0002-9637</issn><eissn>1476-1645</eissn><abstract>Enteric fever is a major public health concern in endemic areas, particularly in infrastructure-limited countries where
Paratyphi A has emerged in increasing proportion of cases. We aimed to evaluate a method to detect
Typhi (
Typhi) and
Paratyphi A (
Paratyphi A) in febrile patients in Bangladesh. We conducted a prospective study enrolling patients with fever > 38°C admitted to two large urban hospitals and two outpatient clinics located in Dhaka, Bangladesh. We developed and evaluated a method combining short culture with a new molecular assay to simultaneously detect and differentiate
Typhi and
Paratyphi A from other
directly from 2 to 4 mL of whole blood in febrile patients (
= 680). A total of 680 cases were enrolled from the four participating sites. An increase in the detection rate (+38.8%) in
Typhi and
. Paratyphi A was observed with a multiplex polymerase chain reaction (PCR) assay, and absence of non-typhoidal
detection was reported. All 45 healthy controls were culture and PCR negative, generating an estimated 92.9% of specificity on clinical samples. When clinical performance was assessed in the absence of blood volume prioritization for testing, a latent class model estimates clinical performance ≥ 95% in sensitivity and specificity with likelihood ratio (LR) LR+ > 10 and LR- < 0.1 for the multiplex PCR assay. The alternative method to blood culture we developed may be useful alone or in combination with culture or serological tests for epidemiological studies in high disease burden settings and should be considered as secondary endpoint test for future vaccine trials.</abstract><cop>United States</cop><pub>Institute of Tropical Medicine</pub><pmid>31287048</pmid><doi>10.4269/ajtmh.18-0992</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-9941-5799</orcidid><orcidid>https://orcid.org/0009-0003-9048-663X</orcidid><orcidid>https://orcid.org/0000-0003-3188-1456</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0002-9637 |
ispartof | The American journal of tropical medicine and hygiene, 2019-01, Vol.101 (3), p.513-520 |
issn | 0002-9637 1476-1645 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6726943 |
source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
subjects | Adolescent Adult Bacteriology Bangladesh Blood Child Child, Preschool Endemic Diseases Humans Life Sciences Microbiology and Parasitology Multiplex Polymerase Chain Reaction - standards Prospective Studies Salmonella Salmonella paratyphi A - immunology Salmonella paratyphi A - isolation & purification Salmonella typhi - immunology Salmonella typhi - isolation & purification Sensitivity and Specificity Serogroup Typhoid Fever - blood Typhoid Fever - diagnosis Typhoid Fever - microbiology Vaccines |
title | Clinical Evaluation of a Multiplex PCR for the Detection of Salmonella enterica Serovars Typhi and Paratyphi A from Blood Specimens in a High-Endemic Setting |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T22%3A33%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clinical%20Evaluation%20of%20a%20Multiplex%20PCR%20for%20the%20Detection%20of%20Salmonella%20enterica%20Serovars%20Typhi%20and%20Paratyphi%20A%20from%20Blood%20Specimens%20in%20a%20High-Endemic%20Setting&rft.jtitle=The%20American%20journal%20of%20tropical%20medicine%20and%20hygiene&rft.au=Pouzol,%20Stephane&rft.date=2019-01-01&rft.volume=101&rft.issue=3&rft.spage=513&rft.epage=520&rft.pages=513-520&rft.issn=0002-9637&rft.eissn=1476-1645&rft_id=info:doi/10.4269/ajtmh.18-0992&rft_dat=%3Cproquest_pubme%3E2306475935%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2306475935&rft_id=info:pmid/31287048&rfr_iscdi=true |