Decellularized liver scaffolds promote liver regeneration after partial hepatectomy
The resectable liver volume is strictly limited and this reduces the number of patients who may be treated. Recently, “tissue/organ decellularization”, a new approach in bioengineering, has been investigated for its ability to produce a native organ scaffold by removing all the viable cells. Such a...
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| Veröffentlicht in: | Scientific reports 2019-08, Vol.9 (1), p.12543-11, Article 12543 |
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| creator | Shimoda, Hirofumi Yagi, Hiroshi Higashi, Hisanobu Tajima, Kazuki Kuroda, Kohei Abe, Yuta Kitago, Minoru Shinoda, Masahiro Kitagawa, Yuko |
| description | The resectable liver volume is strictly limited and this reduces the number of patients who may be treated. Recently, “tissue/organ decellularization”, a new approach in bioengineering, has been investigated for its ability to produce a native organ scaffold by removing all the viable cells. Such a scaffold may support the repair of damaged or injured tissue. The purpose of this study was to evaluate the potential contribution of liver scaffolds to hepatic regeneration after hepatectomy. We sutured the partial liver scaffolds onto the surfaces of partially hepatectomized porcine livers and assessed their therapeutic potential by immune histological analysis at various time points. Animals were sacrificed after surgery and the implanted scaffolds were evaluated for the infiltration of various types of cells. Immune histochemical study showed that blood vessel-like structures, covered with CD31 positive endothelial cells and ALB positive cells, were present in all parts of the scaffolds at days 10 and 28. Blood inflow was observed in some of these ductal structures. More interestingly, CK19 and EpCAM positive cells appeared at day 10. These results suggest that the implantation of a decellularized organ scaffold could promote structural reorganization after liver resection. |
| doi_str_mv | 10.1038/s41598-019-48948-x |
| format | Article |
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Recently, “tissue/organ decellularization”, a new approach in bioengineering, has been investigated for its ability to produce a native organ scaffold by removing all the viable cells. Such a scaffold may support the repair of damaged or injured tissue. The purpose of this study was to evaluate the potential contribution of liver scaffolds to hepatic regeneration after hepatectomy. We sutured the partial liver scaffolds onto the surfaces of partially hepatectomized porcine livers and assessed their therapeutic potential by immune histological analysis at various time points. Animals were sacrificed after surgery and the implanted scaffolds were evaluated for the infiltration of various types of cells. Immune histochemical study showed that blood vessel-like structures, covered with CD31 positive endothelial cells and ALB positive cells, were present in all parts of the scaffolds at days 10 and 28. Blood inflow was observed in some of these ductal structures. More interestingly, CK19 and EpCAM positive cells appeared at day 10. These results suggest that the implantation of a decellularized organ scaffold could promote structural reorganization after liver resection.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-48948-x</identifier><identifier>PMID: 31467359</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/51 ; 14/28 ; 14/63 ; 639/301/54/990 ; 692/4020/4021/288 ; Animals ; Antigens, CD19 - metabolism ; Endothelial cells ; Epithelial Cell Adhesion Molecule - metabolism ; Extracellular Matrix - metabolism ; Hepatectomy ; Humanities and Social Sciences ; Liver ; Liver - physiology ; Liver - surgery ; Liver Regeneration ; multidisciplinary ; Science ; Science (multidisciplinary) ; Surgery ; Swine ; Tissue Engineering - instrumentation ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry</subject><ispartof>Scientific reports, 2019-08, Vol.9 (1), p.12543-11, Article 12543</ispartof><rights>The Author(s) 2019</rights><rights>2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c577t-feb61b2d254f46dbf0a506f537ebbc2ec2aeb7796745b5a1b07fbc118d74167c3</citedby><cites>FETCH-LOGICAL-c577t-feb61b2d254f46dbf0a506f537ebbc2ec2aeb7796745b5a1b07fbc118d74167c3</cites><orcidid>0000-0002-2663-471X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715632/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715632/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27922,27923,41118,42187,51574,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31467359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimoda, Hirofumi</creatorcontrib><creatorcontrib>Yagi, Hiroshi</creatorcontrib><creatorcontrib>Higashi, Hisanobu</creatorcontrib><creatorcontrib>Tajima, Kazuki</creatorcontrib><creatorcontrib>Kuroda, Kohei</creatorcontrib><creatorcontrib>Abe, Yuta</creatorcontrib><creatorcontrib>Kitago, Minoru</creatorcontrib><creatorcontrib>Shinoda, Masahiro</creatorcontrib><creatorcontrib>Kitagawa, Yuko</creatorcontrib><title>Decellularized liver scaffolds promote liver regeneration after partial hepatectomy</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The resectable liver volume is strictly limited and this reduces the number of patients who may be treated. Recently, “tissue/organ decellularization”, a new approach in bioengineering, has been investigated for its ability to produce a native organ scaffold by removing all the viable cells. Such a scaffold may support the repair of damaged or injured tissue. The purpose of this study was to evaluate the potential contribution of liver scaffolds to hepatic regeneration after hepatectomy. We sutured the partial liver scaffolds onto the surfaces of partially hepatectomized porcine livers and assessed their therapeutic potential by immune histological analysis at various time points. Animals were sacrificed after surgery and the implanted scaffolds were evaluated for the infiltration of various types of cells. Immune histochemical study showed that blood vessel-like structures, covered with CD31 positive endothelial cells and ALB positive cells, were present in all parts of the scaffolds at days 10 and 28. Blood inflow was observed in some of these ductal structures. More interestingly, CK19 and EpCAM positive cells appeared at day 10. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimoda, Hirofumi</au><au>Yagi, Hiroshi</au><au>Higashi, Hisanobu</au><au>Tajima, Kazuki</au><au>Kuroda, Kohei</au><au>Abe, Yuta</au><au>Kitago, Minoru</au><au>Shinoda, Masahiro</au><au>Kitagawa, Yuko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decellularized liver scaffolds promote liver regeneration after partial hepatectomy</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-08-29</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>12543</spage><epage>11</epage><pages>12543-11</pages><artnum>12543</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The resectable liver volume is strictly limited and this reduces the number of patients who may be treated. Recently, “tissue/organ decellularization”, a new approach in bioengineering, has been investigated for its ability to produce a native organ scaffold by removing all the viable cells. Such a scaffold may support the repair of damaged or injured tissue. The purpose of this study was to evaluate the potential contribution of liver scaffolds to hepatic regeneration after hepatectomy. We sutured the partial liver scaffolds onto the surfaces of partially hepatectomized porcine livers and assessed their therapeutic potential by immune histological analysis at various time points. Animals were sacrificed after surgery and the implanted scaffolds were evaluated for the infiltration of various types of cells. Immune histochemical study showed that blood vessel-like structures, covered with CD31 positive endothelial cells and ALB positive cells, were present in all parts of the scaffolds at days 10 and 28. Blood inflow was observed in some of these ductal structures. More interestingly, CK19 and EpCAM positive cells appeared at day 10. These results suggest that the implantation of a decellularized organ scaffold could promote structural reorganization after liver resection.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31467359</pmid><doi>10.1038/s41598-019-48948-x</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-2663-471X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 13/51 14/28 14/63 639/301/54/990 692/4020/4021/288 Animals Antigens, CD19 - metabolism Endothelial cells Epithelial Cell Adhesion Molecule - metabolism Extracellular Matrix - metabolism Hepatectomy Humanities and Social Sciences Liver Liver - physiology Liver - surgery Liver Regeneration multidisciplinary Science Science (multidisciplinary) Surgery Swine Tissue Engineering - instrumentation Tissue Engineering - methods Tissue Scaffolds - chemistry |
| title | Decellularized liver scaffolds promote liver regeneration after partial hepatectomy |
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