Single-Cell Quantification of mRNA Expression in The Human Brain

RNA analysis at the cellular resolution in the human brain is challenging. Here, we describe an optimised approach for detecting single RNA transcripts in a cell-type specific manner in frozen human brain tissue using multiplexed fluorescent RNAscope probes. We developed a new robust analytical appr...

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Veröffentlicht in:	Scientific reports 2019-08, Vol.9 (1), p.12353-9, Article 12353
Hauptverfasser:	Jolly, Sarah, Lang, Verena, Koelzer, Viktor Hendrik, Sala Frigerio, Carlo, Magno, Lorenza, Salinas, Patricia C., Whiting, Paul, Palomer, Ernest
Format:	Artikel
Sprache:	eng
Schlagworte:	14/32 38/32 38/77 631/337/572 631/378/340 Alzheimer Disease - genetics Brain Brain - metabolism DNA probes Fluorescent indicators Freezing Gene expression Gene Expression Regulation HeLa Cells Hippocampus - metabolism Hippocampus - pathology Humanities and Social Sciences Humans Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Microglia - metabolism multidisciplinary Receptors, Immunologic - genetics Receptors, Immunologic - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Science Science (multidisciplinary) Single-Cell Analysis Spatial analysis Up-Regulation
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