ChIP and Chips: Introducing the WormPharm for correlative studies employing pharmacology and genome-wide analyses in C. elegans
We present the WormPharm, an automated microfluidic platform that utilizes an axenic medium to culture . The WormPharm is capable of sustaining for extended periods, while recording worm development and growth with high temporal resolution ranging from seconds to minutes over several days to months....
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| Veröffentlicht in: | Journal of biological methods 2016-06, Vol.3 (2), p.e44-e44 |
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| container_title | Journal of biological methods |
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| creator | Doh, Jung H Moore, Andrew B Çelen, İrem Moore, Michael T Sabanayagam, Chandram R |
| description | We present the WormPharm, an automated microfluidic platform that utilizes an axenic medium to culture
. The WormPharm is capable of sustaining
for extended periods, while recording worm development and growth with high temporal resolution ranging from seconds to minutes over several days to months. We demonstrate the utility of the device to monitor
growth in the presence of varying doses of nicotine and alcohol. Furthermore, we show that
cultured in the WormPharm are amendable for high-throughput genomic assays,
chromatin-immunoprecipitation followed by next generation sequencing, and confirm that nematodes grown in monoxenic and axenic cultures exhibit genetic modifications that correlate with observed phenotypes. The WormPharm is a powerful tool for analyzing the effects of chemical, nutritional and environmental variations on organism level responses in conjunction with genome-wide changes in
. |
| doi_str_mv | 10.14440/jbm.2016.109 |
| format | Article |
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. The WormPharm is capable of sustaining
for extended periods, while recording worm development and growth with high temporal resolution ranging from seconds to minutes over several days to months. We demonstrate the utility of the device to monitor
growth in the presence of varying doses of nicotine and alcohol. Furthermore, we show that
cultured in the WormPharm are amendable for high-throughput genomic assays,
chromatin-immunoprecipitation followed by next generation sequencing, and confirm that nematodes grown in monoxenic and axenic cultures exhibit genetic modifications that correlate with observed phenotypes. The WormPharm is a powerful tool for analyzing the effects of chemical, nutritional and environmental variations on organism level responses in conjunction with genome-wide changes in
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. The WormPharm is capable of sustaining
for extended periods, while recording worm development and growth with high temporal resolution ranging from seconds to minutes over several days to months. We demonstrate the utility of the device to monitor
growth in the presence of varying doses of nicotine and alcohol. Furthermore, we show that
cultured in the WormPharm are amendable for high-throughput genomic assays,
chromatin-immunoprecipitation followed by next generation sequencing, and confirm that nematodes grown in monoxenic and axenic cultures exhibit genetic modifications that correlate with observed phenotypes. The WormPharm is a powerful tool for analyzing the effects of chemical, nutritional and environmental variations on organism level responses in conjunction with genome-wide changes in
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. The WormPharm is capable of sustaining
for extended periods, while recording worm development and growth with high temporal resolution ranging from seconds to minutes over several days to months. We demonstrate the utility of the device to monitor
growth in the presence of varying doses of nicotine and alcohol. Furthermore, we show that
cultured in the WormPharm are amendable for high-throughput genomic assays,
chromatin-immunoprecipitation followed by next generation sequencing, and confirm that nematodes grown in monoxenic and axenic cultures exhibit genetic modifications that correlate with observed phenotypes. The WormPharm is a powerful tool for analyzing the effects of chemical, nutritional and environmental variations on organism level responses in conjunction with genome-wide changes in
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| title | ChIP and Chips: Introducing the WormPharm for correlative studies employing pharmacology and genome-wide analyses in C. elegans |
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