Detection of oseltamivir‐resistant zoonotic and animal influenza A viruses using the rapid influenza antiviral resistance test
Mutations in the influenza virus neuraminidase (NA) that cause reduced susceptibility to the NA inhibitor (NAI) oseltamivir may occur naturally or following antiviral treatment. Currently, detection uses either a traditional NA inhibition assay or gene sequencing to identify known markers associated...
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Veröffentlicht in: | Influenza and other respiratory viruses 2019-06, Vol.13 (5), p.522-527 |
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description | Mutations in the influenza virus neuraminidase (NA) that cause reduced susceptibility to the NA inhibitor (NAI) oseltamivir may occur naturally or following antiviral treatment. Currently, detection uses either a traditional NA inhibition assay or gene sequencing to identify known markers associated with reduced inhibition by oseltamivir. Both methods are laborious and require trained personnel. The influenza antiviral resistance test (iART), a prototype system developed by Becton, Dickinson and Company for research use only, offers a rapid and simple method to identify such viruses. This study investigated application of iART to influenza A viruses isolated from non‐human hosts with a variety of NA subtypes (N1‐N9). |
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Currently, detection uses either a traditional NA inhibition assay or gene sequencing to identify known markers associated with reduced inhibition by oseltamivir. Both methods are laborious and require trained personnel. The influenza antiviral resistance test (iART), a prototype system developed by Becton, Dickinson and Company for research use only, offers a rapid and simple method to identify such viruses. This study investigated application of iART to influenza A viruses isolated from non‐human hosts with a variety of NA subtypes (N1‐N9).</description><identifier>ISSN: 1750-2640</identifier><identifier>EISSN: 1750-2659</identifier><identifier>DOI: 10.1111/irv.12661</identifier><identifier>PMID: 31187572</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Antiviral drugs ; avian ; Disease control ; Disease prevention ; Disease resistance ; Exo-a-sialidase ; Gene sequencing ; Health surveillance ; Identification methods ; Influenza ; Influenza A ; influenza A virus ; Influenza viruses ; Inhibition ; Medical laboratories ; Mutation ; Oseltamivir ; Oseltamivir phosphate ; Public health ; R&D ; Research & development ; resistance ; Respiratory diseases ; Short ; Viruses ; Zoonoses ; zoonotic</subject><ispartof>Influenza and other respiratory viruses, 2019-06, Vol.13 (5), p.522-527</ispartof><rights>2019 The Authors. 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subjects | Antiviral drugs avian Disease control Disease prevention Disease resistance Exo-a-sialidase Gene sequencing Health surveillance Identification methods Influenza Influenza A influenza A virus Influenza viruses Inhibition Medical laboratories Mutation Oseltamivir Oseltamivir phosphate Public health R&D Research & development resistance Respiratory diseases Short Viruses Zoonoses zoonotic |
title | Detection of oseltamivir‐resistant zoonotic and animal influenza A viruses using the rapid influenza antiviral resistance test |
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