Detection of oseltamivir‐resistant zoonotic and animal influenza A viruses using the rapid influenza antiviral resistance test

Mutations in the influenza virus neuraminidase (NA) that cause reduced susceptibility to the NA inhibitor (NAI) oseltamivir may occur naturally or following antiviral treatment. Currently, detection uses either a traditional NA inhibition assay or gene sequencing to identify known markers associated...

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Veröffentlicht in:Influenza and other respiratory viruses 2019-06, Vol.13 (5), p.522-527
Hauptverfasser: Hodges, Erin N., Mishin, Vasiliy P., De la Cruz, Juan, Guo, Zhu, Nguyen, Ha T., Fallows, Eric, Stevens, James, Wentworth, David E., Davis, Charles Todd, Gubareva, Larisa V.
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container_end_page 527
container_issue 5
container_start_page 522
container_title Influenza and other respiratory viruses
container_volume 13
creator Hodges, Erin N.
Mishin, Vasiliy P.
De la Cruz, Juan
Guo, Zhu
Nguyen, Ha T.
Fallows, Eric
Stevens, James
Wentworth, David E.
Davis, Charles Todd
Gubareva, Larisa V.
description Mutations in the influenza virus neuraminidase (NA) that cause reduced susceptibility to the NA inhibitor (NAI) oseltamivir may occur naturally or following antiviral treatment. Currently, detection uses either a traditional NA inhibition assay or gene sequencing to identify known markers associated with reduced inhibition by oseltamivir. Both methods are laborious and require trained personnel. The influenza antiviral resistance test (iART), a prototype system developed by Becton, Dickinson and Company for research use only, offers a rapid and simple method to identify such viruses. This study investigated application of iART to influenza A viruses isolated from non‐human hosts with a variety of NA subtypes (N1‐N9).
doi_str_mv 10.1111/irv.12661
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source Wiley-Blackwell Journals; Wiley Open Access; PubMed Central; Directory of Open Access Journals; EZB Electronic Journals Library
subjects Antiviral drugs
avian
Disease control
Disease prevention
Disease resistance
Exo-a-sialidase
Gene sequencing
Health surveillance
Identification methods
Influenza
Influenza A
influenza A virus
Influenza viruses
Inhibition
Medical laboratories
Mutation
Oseltamivir
Oseltamivir phosphate
Public health
R&D
Research & development
resistance
Respiratory diseases
Short
Viruses
Zoonoses
zoonotic
title Detection of oseltamivir‐resistant zoonotic and animal influenza A viruses using the rapid influenza antiviral resistance test
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