Enrichment of CD146 + Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration
Heterogeneity of mesenchymal stem cells (MSCs) influences the cell therapy outcome and the application in tissue engineering. Also, the application of subpopulations of MSCs in cartilage regeneration remains poorly characterized. CD146+ MSCs are identified as the natural ancestors of MSCs and the ex...
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| Veröffentlicht in: | Theranostics 2019-01, Vol.9 (17), p.5105-5121 |
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| creator | Li, Xu Guo, Weimin Zha, Kangkang Jing, Xiaoguang Wang, Mingjie Zhang, Yu Hao, Chunxiang Gao, Shuang Chen, Mingxue Yuan, Zhiguo Wang, Zhenyong Zhang, Xueliang Shen, Shi Li, Haojiang Zhang, Bin Xian, Hai Zhang, Yuan Sui, Xiang Qin, Ling Peng, Jiang Liu, Shuyun Lu, Shibi Guo, Quanyi |
| description | Heterogeneity of mesenchymal stem cells (MSCs) influences the cell therapy outcome and the application in tissue engineering. Also, the application of subpopulations of MSCs in cartilage regeneration remains poorly characterized. CD146+ MSCs are identified as the natural ancestors of MSCs and the expression of CD146 are indicative of greater pluripotency and self-renewal potential. Here, we sorted a CD146
subpopulation from adipose-derived mesenchymal stem cells (ADSCs) for cartilage regeneration.
: CD146
ADSCs were sorted using magnetic activated cell sorting (MACS). Cell surface markers, viability, apoptosis and proliferation were evaluated
. The molecular signatures were analyzed by mRNA and protein expression profiling. By intra-articular injections of cells in a rat osteochondral defect model, we assessed the role of the specific subpopulation in cartilage microenvironment. Finally, CD146
ADSCs were combined with articular cartilage extracellular matrix (ACECM) scaffold for long term (3, 6 months) cartilage repair.
: The enriched CD146
ADSCs showed a high expression of stem cell and pericyte markers, good viability, and immune characteristics to avoid allogeneic rejection. Gene and protein expression profiles revealed that the CD146
ADSCs had different cellular functions especially in regulation inflammation. In a rat model, CD146
ADSCs showed a better inflammation-modulating property in the early stage of intra-articular injections. Importantly, CD146
ADSCs exhibited good biocompatibility with the ACECM scaffold and the CD146
cell-scaffold composites produced less subcutaneous inflammation. The combination of CD146
ADSCs with ACECM scaffold can promote better cartilage regeneration in the long term.
: Our data elucidated the function of the CD146
ADSC subpopulation, established their role in promoting cartilage repair, and highlighted the significance of cell subpopulations as a novel therapeutic for cartilage regeneration. |
| doi_str_mv | 10.7150/thno.33904 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6691381</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2598268491</sourcerecordid><originalsourceid>FETCH-LOGICAL-c406t-778962802e65ad5c2019ce1a03f7e0c80cc7c407032c2bff98bb3987a1653d883</originalsourceid><addsrcrecordid>eNpdkdtu1DAQhiMEolXpDQ-ALHGDQCk-JI59g7RKl4LUCkTh2nKcya6rxN7aTikvwvPWuz1oYW5m5Pnm14z_onhN8ElDavwxrZ0_YUzi6llxSAQTZcMr_HyvPiiOY7zCOSpMJZEviwNGKoIprg6Lv0sXrFlP4BLyA2pPScXRB7To7cZHKE8h2Bvo0WWCCbUwjhFZh1o_ddbpZL1Dv21ao0VI1syjDqjVuRz1CtDyNgVt8sju_UKnYG_RpdHD4McefQ9-8gni3sAPWIGDsJN9VbwY9Bjh-CEfFb8-L3-2X8rzb2df28V5aSrMU9k0QnIqMAVe6742FBNpgGjMhgawEdiYJpMNZtTQbhik6DomRaMJr1kvBDsqPt3rbuZugt7kbwh6VJtgJx3-KK-t-rfj7Fqt_I3iXBImSBZ49yAQ_PUMManJxu3V2oGfo6K0YZTwHBl9-x965efg8nmK1lJQLiq5FXx_T5ngYwwwPC1DsNo6rraOq53jGX6zv_4T-ugvuwPACag-</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2598268491</pqid></control><display><type>article</type><title>Enrichment of CD146 + Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Li, Xu ; Guo, Weimin ; Zha, Kangkang ; Jing, Xiaoguang ; Wang, Mingjie ; Zhang, Yu ; Hao, Chunxiang ; Gao, Shuang ; Chen, Mingxue ; Yuan, Zhiguo ; Wang, Zhenyong ; Zhang, Xueliang ; Shen, Shi ; Li, Haojiang ; Zhang, Bin ; Xian, Hai ; Zhang, Yuan ; Sui, Xiang ; Qin, Ling ; Peng, Jiang ; Liu, Shuyun ; Lu, Shibi ; Guo, Quanyi</creator><creatorcontrib>Li, Xu ; Guo, Weimin ; Zha, Kangkang ; Jing, Xiaoguang ; Wang, Mingjie ; Zhang, Yu ; Hao, Chunxiang ; Gao, Shuang ; Chen, Mingxue ; Yuan, Zhiguo ; Wang, Zhenyong ; Zhang, Xueliang ; Shen, Shi ; Li, Haojiang ; Zhang, Bin ; Xian, Hai ; Zhang, Yuan ; Sui, Xiang ; Qin, Ling ; Peng, Jiang ; Liu, Shuyun ; Lu, Shibi ; Guo, Quanyi</creatorcontrib><description>Heterogeneity of mesenchymal stem cells (MSCs) influences the cell therapy outcome and the application in tissue engineering. Also, the application of subpopulations of MSCs in cartilage regeneration remains poorly characterized. CD146+ MSCs are identified as the natural ancestors of MSCs and the expression of CD146 are indicative of greater pluripotency and self-renewal potential. Here, we sorted a CD146
subpopulation from adipose-derived mesenchymal stem cells (ADSCs) for cartilage regeneration.
: CD146
ADSCs were sorted using magnetic activated cell sorting (MACS). Cell surface markers, viability, apoptosis and proliferation were evaluated
. The molecular signatures were analyzed by mRNA and protein expression profiling. By intra-articular injections of cells in a rat osteochondral defect model, we assessed the role of the specific subpopulation in cartilage microenvironment. Finally, CD146
ADSCs were combined with articular cartilage extracellular matrix (ACECM) scaffold for long term (3, 6 months) cartilage repair.
: The enriched CD146
ADSCs showed a high expression of stem cell and pericyte markers, good viability, and immune characteristics to avoid allogeneic rejection. Gene and protein expression profiles revealed that the CD146
ADSCs had different cellular functions especially in regulation inflammation. In a rat model, CD146
ADSCs showed a better inflammation-modulating property in the early stage of intra-articular injections. Importantly, CD146
ADSCs exhibited good biocompatibility with the ACECM scaffold and the CD146
cell-scaffold composites produced less subcutaneous inflammation. The combination of CD146
ADSCs with ACECM scaffold can promote better cartilage regeneration in the long term.
: Our data elucidated the function of the CD146
ADSC subpopulation, established their role in promoting cartilage repair, and highlighted the significance of cell subpopulations as a novel therapeutic for cartilage regeneration.</description><identifier>ISSN: 1838-7640</identifier><identifier>EISSN: 1838-7640</identifier><identifier>DOI: 10.7150/thno.33904</identifier><identifier>PMID: 31410204</identifier><language>eng</language><publisher>Australia: Ivyspring International Publisher Pty Ltd</publisher><subject>Adipose Tissue - cytology ; Animals ; Antibodies ; Apoptosis ; Arthritis ; Bone marrow ; Cartilage ; Cartilage, Articular - metabolism ; Cartilage, Articular - physiology ; CD146 Antigen - genetics ; CD146 Antigen - metabolism ; Cells, Cultured ; Extracellular matrix ; Extracellular Matrix - chemistry ; Extracellular Matrix - metabolism ; Humans ; Mesenchymal Stem Cell Transplantation - methods ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - metabolism ; Proteins ; Rabbits ; Rats ; Rats, Sprague-Dawley ; Regeneration ; Research Paper ; Software ; Stem cells ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry ; Umbilical cord</subject><ispartof>Theranostics, 2019-01, Vol.9 (17), p.5105-5121</ispartof><rights>2019. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The author(s) 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-778962802e65ad5c2019ce1a03f7e0c80cc7c407032c2bff98bb3987a1653d883</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691381/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691381/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31410204$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xu</creatorcontrib><creatorcontrib>Guo, Weimin</creatorcontrib><creatorcontrib>Zha, Kangkang</creatorcontrib><creatorcontrib>Jing, Xiaoguang</creatorcontrib><creatorcontrib>Wang, Mingjie</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Hao, Chunxiang</creatorcontrib><creatorcontrib>Gao, Shuang</creatorcontrib><creatorcontrib>Chen, Mingxue</creatorcontrib><creatorcontrib>Yuan, Zhiguo</creatorcontrib><creatorcontrib>Wang, Zhenyong</creatorcontrib><creatorcontrib>Zhang, Xueliang</creatorcontrib><creatorcontrib>Shen, Shi</creatorcontrib><creatorcontrib>Li, Haojiang</creatorcontrib><creatorcontrib>Zhang, Bin</creatorcontrib><creatorcontrib>Xian, Hai</creatorcontrib><creatorcontrib>Zhang, Yuan</creatorcontrib><creatorcontrib>Sui, Xiang</creatorcontrib><creatorcontrib>Qin, Ling</creatorcontrib><creatorcontrib>Peng, Jiang</creatorcontrib><creatorcontrib>Liu, Shuyun</creatorcontrib><creatorcontrib>Lu, Shibi</creatorcontrib><creatorcontrib>Guo, Quanyi</creatorcontrib><title>Enrichment of CD146 + Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration</title><title>Theranostics</title><addtitle>Theranostics</addtitle><description>Heterogeneity of mesenchymal stem cells (MSCs) influences the cell therapy outcome and the application in tissue engineering. Also, the application of subpopulations of MSCs in cartilage regeneration remains poorly characterized. CD146+ MSCs are identified as the natural ancestors of MSCs and the expression of CD146 are indicative of greater pluripotency and self-renewal potential. Here, we sorted a CD146
subpopulation from adipose-derived mesenchymal stem cells (ADSCs) for cartilage regeneration.
: CD146
ADSCs were sorted using magnetic activated cell sorting (MACS). Cell surface markers, viability, apoptosis and proliferation were evaluated
. The molecular signatures were analyzed by mRNA and protein expression profiling. By intra-articular injections of cells in a rat osteochondral defect model, we assessed the role of the specific subpopulation in cartilage microenvironment. Finally, CD146
ADSCs were combined with articular cartilage extracellular matrix (ACECM) scaffold for long term (3, 6 months) cartilage repair.
: The enriched CD146
ADSCs showed a high expression of stem cell and pericyte markers, good viability, and immune characteristics to avoid allogeneic rejection. Gene and protein expression profiles revealed that the CD146
ADSCs had different cellular functions especially in regulation inflammation. In a rat model, CD146
ADSCs showed a better inflammation-modulating property in the early stage of intra-articular injections. Importantly, CD146
ADSCs exhibited good biocompatibility with the ACECM scaffold and the CD146
cell-scaffold composites produced less subcutaneous inflammation. The combination of CD146
ADSCs with ACECM scaffold can promote better cartilage regeneration in the long term.
: Our data elucidated the function of the CD146
ADSC subpopulation, established their role in promoting cartilage repair, and highlighted the significance of cell subpopulations as a novel therapeutic for cartilage regeneration.</description><subject>Adipose Tissue - cytology</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Apoptosis</subject><subject>Arthritis</subject><subject>Bone marrow</subject><subject>Cartilage</subject><subject>Cartilage, Articular - metabolism</subject><subject>Cartilage, Articular - physiology</subject><subject>CD146 Antigen - genetics</subject><subject>CD146 Antigen - metabolism</subject><subject>Cells, Cultured</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - chemistry</subject><subject>Extracellular Matrix - metabolism</subject><subject>Humans</subject><subject>Mesenchymal Stem Cell Transplantation - methods</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regeneration</subject><subject>Research Paper</subject><subject>Software</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds - chemistry</subject><subject>Umbilical cord</subject><issn>1838-7640</issn><issn>1838-7640</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkdtu1DAQhiMEolXpDQ-ALHGDQCk-JI59g7RKl4LUCkTh2nKcya6rxN7aTikvwvPWuz1oYW5m5Pnm14z_onhN8ElDavwxrZ0_YUzi6llxSAQTZcMr_HyvPiiOY7zCOSpMJZEviwNGKoIprg6Lv0sXrFlP4BLyA2pPScXRB7To7cZHKE8h2Bvo0WWCCbUwjhFZh1o_ddbpZL1Dv21ao0VI1syjDqjVuRz1CtDyNgVt8sju_UKnYG_RpdHD4McefQ9-8gni3sAPWIGDsJN9VbwY9Bjh-CEfFb8-L3-2X8rzb2df28V5aSrMU9k0QnIqMAVe6742FBNpgGjMhgawEdiYJpMNZtTQbhik6DomRaMJr1kvBDsqPt3rbuZugt7kbwh6VJtgJx3-KK-t-rfj7Fqt_I3iXBImSBZ49yAQ_PUMManJxu3V2oGfo6K0YZTwHBl9-x965efg8nmK1lJQLiq5FXx_T5ngYwwwPC1DsNo6rraOq53jGX6zv_4T-ugvuwPACag-</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Li, Xu</creator><creator>Guo, Weimin</creator><creator>Zha, Kangkang</creator><creator>Jing, Xiaoguang</creator><creator>Wang, Mingjie</creator><creator>Zhang, Yu</creator><creator>Hao, Chunxiang</creator><creator>Gao, Shuang</creator><creator>Chen, Mingxue</creator><creator>Yuan, Zhiguo</creator><creator>Wang, Zhenyong</creator><creator>Zhang, Xueliang</creator><creator>Shen, Shi</creator><creator>Li, Haojiang</creator><creator>Zhang, Bin</creator><creator>Xian, Hai</creator><creator>Zhang, Yuan</creator><creator>Sui, Xiang</creator><creator>Qin, Ling</creator><creator>Peng, Jiang</creator><creator>Liu, Shuyun</creator><creator>Lu, Shibi</creator><creator>Guo, Quanyi</creator><general>Ivyspring International Publisher Pty Ltd</general><general>Ivyspring International Publisher</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190101</creationdate><title>Enrichment of CD146 + Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration</title><author>Li, Xu ; Guo, Weimin ; Zha, Kangkang ; Jing, Xiaoguang ; Wang, Mingjie ; Zhang, Yu ; Hao, Chunxiang ; Gao, Shuang ; Chen, Mingxue ; Yuan, Zhiguo ; Wang, Zhenyong ; Zhang, Xueliang ; Shen, Shi ; Li, Haojiang ; Zhang, Bin ; Xian, Hai ; Zhang, Yuan ; Sui, Xiang ; Qin, Ling ; Peng, Jiang ; Liu, Shuyun ; Lu, Shibi ; Guo, Quanyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-778962802e65ad5c2019ce1a03f7e0c80cc7c407032c2bff98bb3987a1653d883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adipose Tissue - cytology</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Apoptosis</topic><topic>Arthritis</topic><topic>Bone marrow</topic><topic>Cartilage</topic><topic>Cartilage, Articular - metabolism</topic><topic>Cartilage, Articular - physiology</topic><topic>CD146 Antigen - genetics</topic><topic>CD146 Antigen - metabolism</topic><topic>Cells, Cultured</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - chemistry</topic><topic>Extracellular Matrix - metabolism</topic><topic>Humans</topic><topic>Mesenchymal Stem Cell Transplantation - methods</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>Proteins</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Regeneration</topic><topic>Research Paper</topic><topic>Software</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds - chemistry</topic><topic>Umbilical cord</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xu</creatorcontrib><creatorcontrib>Guo, Weimin</creatorcontrib><creatorcontrib>Zha, Kangkang</creatorcontrib><creatorcontrib>Jing, Xiaoguang</creatorcontrib><creatorcontrib>Wang, Mingjie</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Hao, Chunxiang</creatorcontrib><creatorcontrib>Gao, Shuang</creatorcontrib><creatorcontrib>Chen, Mingxue</creatorcontrib><creatorcontrib>Yuan, Zhiguo</creatorcontrib><creatorcontrib>Wang, Zhenyong</creatorcontrib><creatorcontrib>Zhang, Xueliang</creatorcontrib><creatorcontrib>Shen, Shi</creatorcontrib><creatorcontrib>Li, Haojiang</creatorcontrib><creatorcontrib>Zhang, Bin</creatorcontrib><creatorcontrib>Xian, Hai</creatorcontrib><creatorcontrib>Zhang, Yuan</creatorcontrib><creatorcontrib>Sui, Xiang</creatorcontrib><creatorcontrib>Qin, Ling</creatorcontrib><creatorcontrib>Peng, Jiang</creatorcontrib><creatorcontrib>Liu, Shuyun</creatorcontrib><creatorcontrib>Lu, Shibi</creatorcontrib><creatorcontrib>Guo, Quanyi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Theranostics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xu</au><au>Guo, Weimin</au><au>Zha, Kangkang</au><au>Jing, Xiaoguang</au><au>Wang, Mingjie</au><au>Zhang, Yu</au><au>Hao, Chunxiang</au><au>Gao, Shuang</au><au>Chen, Mingxue</au><au>Yuan, Zhiguo</au><au>Wang, Zhenyong</au><au>Zhang, Xueliang</au><au>Shen, Shi</au><au>Li, Haojiang</au><au>Zhang, Bin</au><au>Xian, Hai</au><au>Zhang, Yuan</au><au>Sui, Xiang</au><au>Qin, Ling</au><au>Peng, Jiang</au><au>Liu, Shuyun</au><au>Lu, Shibi</au><au>Guo, Quanyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enrichment of CD146 + Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration</atitle><jtitle>Theranostics</jtitle><addtitle>Theranostics</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>9</volume><issue>17</issue><spage>5105</spage><epage>5121</epage><pages>5105-5121</pages><issn>1838-7640</issn><eissn>1838-7640</eissn><abstract>Heterogeneity of mesenchymal stem cells (MSCs) influences the cell therapy outcome and the application in tissue engineering. Also, the application of subpopulations of MSCs in cartilage regeneration remains poorly characterized. CD146+ MSCs are identified as the natural ancestors of MSCs and the expression of CD146 are indicative of greater pluripotency and self-renewal potential. Here, we sorted a CD146
subpopulation from adipose-derived mesenchymal stem cells (ADSCs) for cartilage regeneration.
: CD146
ADSCs were sorted using magnetic activated cell sorting (MACS). Cell surface markers, viability, apoptosis and proliferation were evaluated
. The molecular signatures were analyzed by mRNA and protein expression profiling. By intra-articular injections of cells in a rat osteochondral defect model, we assessed the role of the specific subpopulation in cartilage microenvironment. Finally, CD146
ADSCs were combined with articular cartilage extracellular matrix (ACECM) scaffold for long term (3, 6 months) cartilage repair.
: The enriched CD146
ADSCs showed a high expression of stem cell and pericyte markers, good viability, and immune characteristics to avoid allogeneic rejection. Gene and protein expression profiles revealed that the CD146
ADSCs had different cellular functions especially in regulation inflammation. In a rat model, CD146
ADSCs showed a better inflammation-modulating property in the early stage of intra-articular injections. Importantly, CD146
ADSCs exhibited good biocompatibility with the ACECM scaffold and the CD146
cell-scaffold composites produced less subcutaneous inflammation. The combination of CD146
ADSCs with ACECM scaffold can promote better cartilage regeneration in the long term.
: Our data elucidated the function of the CD146
ADSC subpopulation, established their role in promoting cartilage repair, and highlighted the significance of cell subpopulations as a novel therapeutic for cartilage regeneration.</abstract><cop>Australia</cop><pub>Ivyspring International Publisher Pty Ltd</pub><pmid>31410204</pmid><doi>10.7150/thno.33904</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Adipose Tissue - cytology Animals Antibodies Apoptosis Arthritis Bone marrow Cartilage Cartilage, Articular - metabolism Cartilage, Articular - physiology CD146 Antigen - genetics CD146 Antigen - metabolism Cells, Cultured Extracellular matrix Extracellular Matrix - chemistry Extracellular Matrix - metabolism Humans Mesenchymal Stem Cell Transplantation - methods Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - metabolism Proteins Rabbits Rats Rats, Sprague-Dawley Regeneration Research Paper Software Stem cells Tissue engineering Tissue Engineering - methods Tissue Scaffolds - chemistry Umbilical cord |
| title | Enrichment of CD146 + Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration |
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