CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective
Filamentous fungi play an important role in human health and industrial/agricultural production. With the increasing number of full genomes available for fungal species, the study of filamentous fungi has brought about a wider range of genetic manipulation opportunities. However, the utilization of...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2019-09, Vol.103 (17), p.6919-6932 |
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| container_title | Applied microbiology and biotechnology |
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| creator | Song, Runjie Zhai, Qing Sun, Lu Huang, Enxia Zhang, Yu Zhu, Yanli Guo, Qingyun Tian, Yanan Zhao, Baoyu Lu, Hao |
| description | Filamentous fungi play an important role in human health and industrial/agricultural production. With the increasing number of full genomes available for fungal species, the study of filamentous fungi has brought about a wider range of genetic manipulation opportunities. However, the utilization of traditional methods to study fungi is time consuming and laborious. Recent rapid progress and wide application of a versatile genome editing technology, i.e., the CRISPR (clustered regularly interspaced short palindromic repeat)–Cas9 (CRISPR-related nuclease 9) system, has revolutionized biological research and has many innovative applications in a wide range of fields showing great promise in research and application of filamentous fungi. In this review, we introduce the CRISPR/Cas9 genome editing technology focusing on its application in research of filamentous fungi and we discuss the general considerations of genome editing using CRISPR/Cas9 system illustrating vector construction, multiple editing strategies, technical consideration of different sizes of homology arms on genome editing efficiency, off-target effects, and different transformation methodologies. In addition, we discuss the challenges encountered using CRISPR/Cas9 technology and give the perspectives of future applications of CRISPR/Cas9 technology for basic research and practical application of filamentous fungi. |
| doi_str_mv | 10.1007/s00253-019-10007-w |
| format | Article |
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With the increasing number of full genomes available for fungal species, the study of filamentous fungi has brought about a wider range of genetic manipulation opportunities. However, the utilization of traditional methods to study fungi is time consuming and laborious. Recent rapid progress and wide application of a versatile genome editing technology, i.e., the CRISPR (clustered regularly interspaced short palindromic repeat)–Cas9 (CRISPR-related nuclease 9) system, has revolutionized biological research and has many innovative applications in a wide range of fields showing great promise in research and application of filamentous fungi. In this review, we introduce the CRISPR/Cas9 genome editing technology focusing on its application in research of filamentous fungi and we discuss the general considerations of genome editing using CRISPR/Cas9 system illustrating vector construction, multiple editing strategies, technical consideration of different sizes of homology arms on genome editing efficiency, off-target effects, and different transformation methodologies. In addition, we discuss the challenges encountered using CRISPR/Cas9 technology and give the perspectives of future applications of CRISPR/Cas9 technology for basic research and practical application of filamentous fungi.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-019-10007-w</identifier><identifier>PMID: 31332488</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Agricultural production ; Biological research ; Biomedical and Life Sciences ; Biotechnology ; CRISPR ; CRISPR-Associated Protein 9 - genetics ; CRISPR-Cas systems ; CRISPR-Cas Systems - genetics ; Editing ; enzymes ; Fungi ; Fungi - genetics ; Gene Editing ; Gene Targeting ; Genetic engineering ; Genetic research ; Genetic transformation ; genome ; Genome editing ; Genome, Fungal - genetics ; Genomes ; Genomics ; Homology ; human health ; Industrial Microbiology ; Life Sciences ; Microbial Genetics and Genomics ; Microbiology ; Mini-Review ; Mutation ; Nuclease ; RNA, Guide, CRISPR-Cas Systems - genetics ; Technology application ; Transformation, Genetic</subject><ispartof>Applied microbiology and biotechnology, 2019-09, Vol.103 (17), p.6919-6932</ispartof><rights>The Author(s) 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Applied Microbiology and Biotechnology is a copyright of Springer, (2019). All Rights Reserved. © 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c645t-4f431e166d14176c0265a27a97fde9b7427e54cea956a4d471cc6014dbe7fca13</citedby><cites>FETCH-LOGICAL-c645t-4f431e166d14176c0265a27a97fde9b7427e54cea956a4d471cc6014dbe7fca13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-019-10007-w$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-019-10007-w$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31332488$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Runjie</creatorcontrib><creatorcontrib>Zhai, Qing</creatorcontrib><creatorcontrib>Sun, Lu</creatorcontrib><creatorcontrib>Huang, Enxia</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Zhu, Yanli</creatorcontrib><creatorcontrib>Guo, Qingyun</creatorcontrib><creatorcontrib>Tian, Yanan</creatorcontrib><creatorcontrib>Zhao, Baoyu</creatorcontrib><creatorcontrib>Lu, Hao</creatorcontrib><title>CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Filamentous fungi play an important role in human health and industrial/agricultural production. With the increasing number of full genomes available for fungal species, the study of filamentous fungi has brought about a wider range of genetic manipulation opportunities. However, the utilization of traditional methods to study fungi is time consuming and laborious. Recent rapid progress and wide application of a versatile genome editing technology, i.e., the CRISPR (clustered regularly interspaced short palindromic repeat)–Cas9 (CRISPR-related nuclease 9) system, has revolutionized biological research and has many innovative applications in a wide range of fields showing great promise in research and application of filamentous fungi. In this review, we introduce the CRISPR/Cas9 genome editing technology focusing on its application in research of filamentous fungi and we discuss the general considerations of genome editing using CRISPR/Cas9 system illustrating vector construction, multiple editing strategies, technical consideration of different sizes of homology arms on genome editing efficiency, off-target effects, and different transformation methodologies. In addition, we discuss the challenges encountered using CRISPR/Cas9 technology and give the perspectives of future applications of CRISPR/Cas9 technology for basic research and practical application of filamentous fungi.</description><subject>Agricultural production</subject><subject>Biological research</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>CRISPR</subject><subject>CRISPR-Associated Protein 9 - genetics</subject><subject>CRISPR-Cas systems</subject><subject>CRISPR-Cas Systems - genetics</subject><subject>Editing</subject><subject>enzymes</subject><subject>Fungi</subject><subject>Fungi - genetics</subject><subject>Gene Editing</subject><subject>Gene Targeting</subject><subject>Genetic engineering</subject><subject>Genetic research</subject><subject>Genetic transformation</subject><subject>genome</subject><subject>Genome editing</subject><subject>Genome, Fungal - genetics</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Homology</subject><subject>human health</subject><subject>Industrial Microbiology</subject><subject>Life Sciences</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Mini-Review</subject><subject>Mutation</subject><subject>Nuclease</subject><subject>RNA, Guide, CRISPR-Cas Systems - 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With the increasing number of full genomes available for fungal species, the study of filamentous fungi has brought about a wider range of genetic manipulation opportunities. However, the utilization of traditional methods to study fungi is time consuming and laborious. Recent rapid progress and wide application of a versatile genome editing technology, i.e., the CRISPR (clustered regularly interspaced short palindromic repeat)–Cas9 (CRISPR-related nuclease 9) system, has revolutionized biological research and has many innovative applications in a wide range of fields showing great promise in research and application of filamentous fungi. In this review, we introduce the CRISPR/Cas9 genome editing technology focusing on its application in research of filamentous fungi and we discuss the general considerations of genome editing using CRISPR/Cas9 system illustrating vector construction, multiple editing strategies, technical consideration of different sizes of homology arms on genome editing efficiency, off-target effects, and different transformation methodologies. In addition, we discuss the challenges encountered using CRISPR/Cas9 technology and give the perspectives of future applications of CRISPR/Cas9 technology for basic research and practical application of filamentous fungi.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31332488</pmid><doi>10.1007/s00253-019-10007-w</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied microbiology and biotechnology, 2019-09, Vol.103 (17), p.6919-6932 |
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| subjects | Agricultural production Biological research Biomedical and Life Sciences Biotechnology CRISPR CRISPR-Associated Protein 9 - genetics CRISPR-Cas systems CRISPR-Cas Systems - genetics Editing enzymes Fungi Fungi - genetics Gene Editing Gene Targeting Genetic engineering Genetic research Genetic transformation genome Genome editing Genome, Fungal - genetics Genomes Genomics Homology human health Industrial Microbiology Life Sciences Microbial Genetics and Genomics Microbiology Mini-Review Mutation Nuclease RNA, Guide, CRISPR-Cas Systems - genetics Technology application Transformation, Genetic |
| title | CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective |
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