Cucumber mosaic virus-induced gene silencing in banana
Banana ( Musa spp.) is one of the world’s most important staple and cash crops. Despite accumulating genetic and transcriptomic data, low transformation efficiency in agronomically important Musa spp. render translational researches in banana difficult by using conventional knockout approaches. To d...
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| Veröffentlicht in: | Scientific reports 2019-08, Vol.9 (1), p.11553-9, Article 11553 |
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| creator | Tzean, Yuh Lee, Ming-Chi Jan, Hsiao-Hsuan Chiu, Yi-Shu Tu, Tsui-Chin Hou, Bo-Han Chen, Ho-Ming Chou, Chun-Nan Yeh, Hsin-Hung |
| description | Banana (
Musa
spp.) is one of the world’s most important staple and cash crops. Despite accumulating genetic and transcriptomic data, low transformation efficiency in agronomically important
Musa
spp. render translational researches in banana difficult by using conventional knockout approaches. To develop tools for translational research in bananas, we developed a virus induced-gene silencing (VIGS) system based on a banana-infecting cucumber mosaic virus (CMV) isolate, CMV 20. CMV 20 genomic RNA 1, 2, and 3, were separately cloned in
Agrobacterium
pJL89 binary vectors, and a cloning site was introduced on RNA 2 immediately after the 2a open reading frame to insert the gene targeted for silencing. An efficient
Agrobacterium
inoculation method was developed for banana, which enabled the CMV 20 VIGS vector infection rate to reach 95% in our experiments. CMV 20-based silencing of
Musa acuminata
cv. Cavendish (AAA group)
glutamate 1-semialdehyde aminotransferase
(
MaGSA
) produced a typical chlorotic phenotype and silencing of
M. acuminata phytoene desaturase
(
MaPDS
) produced a photobleachnig phenotype. We show this approach efficiently reduced
GSA
and
PDS
transcripts to 10% and 18% of the control, respectively. The high infection rate and extended silencing of this VIGS system will provide an invaluable tool to accelerate functional genomic studies in banana. |
| doi_str_mv | 10.1038/s41598-019-47962-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6689018</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2271834980</sourcerecordid><originalsourceid>FETCH-LOGICAL-c577t-e5fa13815a67771169d81d9b3c41fb33f91f81a0046a691b8e793595320ee7533</originalsourceid><addsrcrecordid>eNp9kU9P3DAQxa2qqKyAL8ChitRLLwGPHf-7VKpWFJCQuMDZcpzJ1qvE2dobpH57vF2gWw71HGxpfn6e50fIOdALoFxf5gaE0TUFUzfKSFbzD2TBaCNqxhn7eHA-Jmc5r2lZgpkGzCdyzIEbI0EviFzOfh5bTNU4ZRd89RTSnOsQu9ljV60wYpXDgNGHuKpCrFoXS52So94NGc9e9hPy-OPqYXlT391f3y6_39VeKLWtUfQOuAbhpFIKQJpOQ2da7hvoW857A70GR2kjnTTQalSGCyM4o4hKcH5Cvu11N3M7YucxbpMb7CaF0aXfdnLB_tuJ4addTU9WSm0o6CLw9UUgTb9mzFs7huxxGFzEac6WMVWoxmha0C_v0PU0p1js7SgqoCm_WSi2p3yack7Yvw0D1O6SsftkbEnG_knG7mx8PrTxduU1hwLwPZBLK64w_X37P7LPgJKXgA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2270514232</pqid></control><display><type>article</type><title>Cucumber mosaic virus-induced gene silencing in banana</title><source>MEDLINE</source><source>Nature Free</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Springer Nature OA/Free Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Tzean, Yuh ; Lee, Ming-Chi ; Jan, Hsiao-Hsuan ; Chiu, Yi-Shu ; Tu, Tsui-Chin ; Hou, Bo-Han ; Chen, Ho-Ming ; Chou, Chun-Nan ; Yeh, Hsin-Hung</creator><creatorcontrib>Tzean, Yuh ; Lee, Ming-Chi ; Jan, Hsiao-Hsuan ; Chiu, Yi-Shu ; Tu, Tsui-Chin ; Hou, Bo-Han ; Chen, Ho-Ming ; Chou, Chun-Nan ; Yeh, Hsin-Hung</creatorcontrib><description>Banana (
Musa
spp.) is one of the world’s most important staple and cash crops. Despite accumulating genetic and transcriptomic data, low transformation efficiency in agronomically important
Musa
spp. render translational researches in banana difficult by using conventional knockout approaches. To develop tools for translational research in bananas, we developed a virus induced-gene silencing (VIGS) system based on a banana-infecting cucumber mosaic virus (CMV) isolate, CMV 20. CMV 20 genomic RNA 1, 2, and 3, were separately cloned in
Agrobacterium
pJL89 binary vectors, and a cloning site was introduced on RNA 2 immediately after the 2a open reading frame to insert the gene targeted for silencing. An efficient
Agrobacterium
inoculation method was developed for banana, which enabled the CMV 20 VIGS vector infection rate to reach 95% in our experiments. CMV 20-based silencing of
Musa acuminata
cv. Cavendish (AAA group)
glutamate 1-semialdehyde aminotransferase
(
MaGSA
) produced a typical chlorotic phenotype and silencing of
M. acuminata phytoene desaturase
(
MaPDS
) produced a photobleachnig phenotype. We show this approach efficiently reduced
GSA
and
PDS
transcripts to 10% and 18% of the control, respectively. The high infection rate and extended silencing of this VIGS system will provide an invaluable tool to accelerate functional genomic studies in banana.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-47962-3</identifier><identifier>PMID: 31399618</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/77 ; 38/89 ; 38/90 ; 38/91 ; 45/41 ; 631/61/191/505 ; 631/61/32 ; Agrobacterium ; Agrobacterium - genetics ; Bananas ; Cloning ; Cloning vectors ; CRISPR ; Cucumovirus - genetics ; Desaturase ; Enzymes ; Gene expression ; Gene Expression Regulation, Plant ; Gene Silencing ; Genes, Plant ; Genetic transformation ; Genetic Vectors - genetics ; Genomes ; Humanities and Social Sciences ; Infections ; Inoculation ; multidisciplinary ; Musa - genetics ; Phenotypes ; Plant Proteins - genetics ; Plant virus diseases ; Proteins ; Ribonucleic acid ; RNA ; Science ; Science (multidisciplinary) ; Translation</subject><ispartof>Scientific reports, 2019-08, Vol.9 (1), p.11553-9, Article 11553</ispartof><rights>The Author(s) 2019</rights><rights>2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c577t-e5fa13815a67771169d81d9b3c41fb33f91f81a0046a691b8e793595320ee7533</citedby><cites>FETCH-LOGICAL-c577t-e5fa13815a67771169d81d9b3c41fb33f91f81a0046a691b8e793595320ee7533</cites><orcidid>0000-0001-5979-0121</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689018/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689018/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31399618$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tzean, Yuh</creatorcontrib><creatorcontrib>Lee, Ming-Chi</creatorcontrib><creatorcontrib>Jan, Hsiao-Hsuan</creatorcontrib><creatorcontrib>Chiu, Yi-Shu</creatorcontrib><creatorcontrib>Tu, Tsui-Chin</creatorcontrib><creatorcontrib>Hou, Bo-Han</creatorcontrib><creatorcontrib>Chen, Ho-Ming</creatorcontrib><creatorcontrib>Chou, Chun-Nan</creatorcontrib><creatorcontrib>Yeh, Hsin-Hung</creatorcontrib><title>Cucumber mosaic virus-induced gene silencing in banana</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Banana (
Musa
spp.) is one of the world’s most important staple and cash crops. Despite accumulating genetic and transcriptomic data, low transformation efficiency in agronomically important
Musa
spp. render translational researches in banana difficult by using conventional knockout approaches. To develop tools for translational research in bananas, we developed a virus induced-gene silencing (VIGS) system based on a banana-infecting cucumber mosaic virus (CMV) isolate, CMV 20. CMV 20 genomic RNA 1, 2, and 3, were separately cloned in
Agrobacterium
pJL89 binary vectors, and a cloning site was introduced on RNA 2 immediately after the 2a open reading frame to insert the gene targeted for silencing. An efficient
Agrobacterium
inoculation method was developed for banana, which enabled the CMV 20 VIGS vector infection rate to reach 95% in our experiments. CMV 20-based silencing of
Musa acuminata
cv. Cavendish (AAA group)
glutamate 1-semialdehyde aminotransferase
(
MaGSA
) produced a typical chlorotic phenotype and silencing of
M. acuminata phytoene desaturase
(
MaPDS
) produced a photobleachnig phenotype. We show this approach efficiently reduced
GSA
and
PDS
transcripts to 10% and 18% of the control, respectively. The high infection rate and extended silencing of this VIGS system will provide an invaluable tool to accelerate functional genomic studies in banana.</description><subject>38/77</subject><subject>38/89</subject><subject>38/90</subject><subject>38/91</subject><subject>45/41</subject><subject>631/61/191/505</subject><subject>631/61/32</subject><subject>Agrobacterium</subject><subject>Agrobacterium - genetics</subject><subject>Bananas</subject><subject>Cloning</subject><subject>Cloning vectors</subject><subject>CRISPR</subject><subject>Cucumovirus - genetics</subject><subject>Desaturase</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Silencing</subject><subject>Genes, Plant</subject><subject>Genetic transformation</subject><subject>Genetic Vectors - genetics</subject><subject>Genomes</subject><subject>Humanities and Social Sciences</subject><subject>Infections</subject><subject>Inoculation</subject><subject>multidisciplinary</subject><subject>Musa - genetics</subject><subject>Phenotypes</subject><subject>Plant Proteins - genetics</subject><subject>Plant virus diseases</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Translation</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU9P3DAQxa2qqKyAL8ChitRLLwGPHf-7VKpWFJCQuMDZcpzJ1qvE2dobpH57vF2gWw71HGxpfn6e50fIOdALoFxf5gaE0TUFUzfKSFbzD2TBaCNqxhn7eHA-Jmc5r2lZgpkGzCdyzIEbI0EviFzOfh5bTNU4ZRd89RTSnOsQu9ljV60wYpXDgNGHuKpCrFoXS52So94NGc9e9hPy-OPqYXlT391f3y6_39VeKLWtUfQOuAbhpFIKQJpOQ2da7hvoW857A70GR2kjnTTQalSGCyM4o4hKcH5Cvu11N3M7YucxbpMb7CaF0aXfdnLB_tuJ4addTU9WSm0o6CLw9UUgTb9mzFs7huxxGFzEac6WMVWoxmha0C_v0PU0p1js7SgqoCm_WSi2p3yack7Yvw0D1O6SsftkbEnG_knG7mx8PrTxduU1hwLwPZBLK64w_X37P7LPgJKXgA</recordid><startdate>20190809</startdate><enddate>20190809</enddate><creator>Tzean, Yuh</creator><creator>Lee, Ming-Chi</creator><creator>Jan, Hsiao-Hsuan</creator><creator>Chiu, Yi-Shu</creator><creator>Tu, Tsui-Chin</creator><creator>Hou, Bo-Han</creator><creator>Chen, Ho-Ming</creator><creator>Chou, Chun-Nan</creator><creator>Yeh, Hsin-Hung</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5979-0121</orcidid></search><sort><creationdate>20190809</creationdate><title>Cucumber mosaic virus-induced gene silencing in banana</title><author>Tzean, Yuh ; Lee, Ming-Chi ; Jan, Hsiao-Hsuan ; Chiu, Yi-Shu ; Tu, Tsui-Chin ; Hou, Bo-Han ; Chen, Ho-Ming ; Chou, Chun-Nan ; Yeh, Hsin-Hung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c577t-e5fa13815a67771169d81d9b3c41fb33f91f81a0046a691b8e793595320ee7533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>38/77</topic><topic>38/89</topic><topic>38/90</topic><topic>38/91</topic><topic>45/41</topic><topic>631/61/191/505</topic><topic>631/61/32</topic><topic>Agrobacterium</topic><topic>Agrobacterium - genetics</topic><topic>Bananas</topic><topic>Cloning</topic><topic>Cloning vectors</topic><topic>CRISPR</topic><topic>Cucumovirus - genetics</topic><topic>Desaturase</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Silencing</topic><topic>Genes, Plant</topic><topic>Genetic transformation</topic><topic>Genetic Vectors - genetics</topic><topic>Genomes</topic><topic>Humanities and Social Sciences</topic><topic>Infections</topic><topic>Inoculation</topic><topic>multidisciplinary</topic><topic>Musa - genetics</topic><topic>Phenotypes</topic><topic>Plant Proteins - genetics</topic><topic>Plant virus diseases</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Translation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tzean, Yuh</creatorcontrib><creatorcontrib>Lee, Ming-Chi</creatorcontrib><creatorcontrib>Jan, Hsiao-Hsuan</creatorcontrib><creatorcontrib>Chiu, Yi-Shu</creatorcontrib><creatorcontrib>Tu, Tsui-Chin</creatorcontrib><creatorcontrib>Hou, Bo-Han</creatorcontrib><creatorcontrib>Chen, Ho-Ming</creatorcontrib><creatorcontrib>Chou, Chun-Nan</creatorcontrib><creatorcontrib>Yeh, Hsin-Hung</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tzean, Yuh</au><au>Lee, Ming-Chi</au><au>Jan, Hsiao-Hsuan</au><au>Chiu, Yi-Shu</au><au>Tu, Tsui-Chin</au><au>Hou, Bo-Han</au><au>Chen, Ho-Ming</au><au>Chou, Chun-Nan</au><au>Yeh, Hsin-Hung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cucumber mosaic virus-induced gene silencing in banana</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-08-09</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>11553</spage><epage>9</epage><pages>11553-9</pages><artnum>11553</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Banana (
Musa
spp.) is one of the world’s most important staple and cash crops. Despite accumulating genetic and transcriptomic data, low transformation efficiency in agronomically important
Musa
spp. render translational researches in banana difficult by using conventional knockout approaches. To develop tools for translational research in bananas, we developed a virus induced-gene silencing (VIGS) system based on a banana-infecting cucumber mosaic virus (CMV) isolate, CMV 20. CMV 20 genomic RNA 1, 2, and 3, were separately cloned in
Agrobacterium
pJL89 binary vectors, and a cloning site was introduced on RNA 2 immediately after the 2a open reading frame to insert the gene targeted for silencing. An efficient
Agrobacterium
inoculation method was developed for banana, which enabled the CMV 20 VIGS vector infection rate to reach 95% in our experiments. CMV 20-based silencing of
Musa acuminata
cv. Cavendish (AAA group)
glutamate 1-semialdehyde aminotransferase
(
MaGSA
) produced a typical chlorotic phenotype and silencing of
M. acuminata phytoene desaturase
(
MaPDS
) produced a photobleachnig phenotype. We show this approach efficiently reduced
GSA
and
PDS
transcripts to 10% and 18% of the control, respectively. The high infection rate and extended silencing of this VIGS system will provide an invaluable tool to accelerate functional genomic studies in banana.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31399618</pmid><doi>10.1038/s41598-019-47962-3</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-5979-0121</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Nature Free; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; Springer Nature OA/Free Journals; Free Full-Text Journals in Chemistry |
| subjects | 38/77 38/89 38/90 38/91 45/41 631/61/191/505 631/61/32 Agrobacterium Agrobacterium - genetics Bananas Cloning Cloning vectors CRISPR Cucumovirus - genetics Desaturase Enzymes Gene expression Gene Expression Regulation, Plant Gene Silencing Genes, Plant Genetic transformation Genetic Vectors - genetics Genomes Humanities and Social Sciences Infections Inoculation multidisciplinary Musa - genetics Phenotypes Plant Proteins - genetics Plant virus diseases Proteins Ribonucleic acid RNA Science Science (multidisciplinary) Translation |
| title | Cucumber mosaic virus-induced gene silencing in banana |
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