Impact of acetate- or citrate-acidified bicarbonate dialysate on ex vivo aorta wall calcification
Vascular calcification is highly prevalent in patients with chronic hemodialysis. Increased acetatemia during hemodialysis sessions using acetate-acidified bicarbonate has also been associated with several abnormalities, By contrast, these abnormalities were not induced by citrate-acidified bicarbon...
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| description | Vascular calcification is highly prevalent in patients with chronic hemodialysis. Increased acetatemia during hemodialysis sessions using acetate-acidified bicarbonate has also been associated with several abnormalities, By contrast, these abnormalities were not induced by citrate-acidified bicarbonate dialysis. Moreover, citrate is biocompatible alternative to acetate in dialysis fluid. However, the effects of citrate on vascular calcification during hemodialysis had not been studied in detail. This study analyzed herein the effects of acetate- or citrate-acidified bicarbonate dialysis on vascular calcification. Citrate has been shown to inhibit calcification in urine in hemodialysis patients. Therefore, our hypothesis is that citrate-acidified bicarbonate dialysis could reduce vascular calcification. Blood samples before and after hemodialysis from patients on acetate- or citrate-acidified bicarbonate dialysis were collected in heparin-containing tubes (n = 35 and n = 25 respectively). To explore the effect of pre- and post-dialysis plasmatic bicarbonate and citrate on vascular calcification, rats aortic rings cultured
ex vivo
in Minimum Essential Medium containing 0.1% FBS and 45-calcium as radiotracer were used (n = 24). After 7 days of incubation aortic rings were dried, weighed and radioactivity was measured via liquid scintillation counting. Bicarbonate levels increase calcium accumulation in rat aortic wall in a dose-response manner (pH = 7.4). Moreover, citrate prevents calcium accumulation, with a mean inhibitor concentration (IC
50
) value of 733 µmol/L. During acetate-acidified bicarbonate dialysis, bicarbonate and citrate levels in plasma increase (22.29 ± 3.59 versus 28.63 ± 3.56 mmol/L; p |
| doi_str_mv | 10.1038/s41598-019-47934-7 |
| format | Article |
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ex vivo
in Minimum Essential Medium containing 0.1% FBS and 45-calcium as radiotracer were used (n = 24). After 7 days of incubation aortic rings were dried, weighed and radioactivity was measured via liquid scintillation counting. Bicarbonate levels increase calcium accumulation in rat aortic wall in a dose-response manner (pH = 7.4). Moreover, citrate prevents calcium accumulation, with a mean inhibitor concentration (IC
50
) value of 733 µmol/L. During acetate-acidified bicarbonate dialysis, bicarbonate and citrate levels in plasma increase (22.29 ± 3.59 versus 28.63 ± 3.56 mmol/L; p < 0.001) and decrease (133.3 ± 53.6 versus 87.49 ± 32.3 µmol/L, p < 0.001), respectively. These changes in pos-hemodialysis plasma significantly (p < 0.001) alter calcium accumulation in the aortic wall (38.9% higher). Moreover, citrate-acidified bicarbonate dialysis increases post-hemodialysis citrate levels 5-fold (145 ± 79.8 versus 771.6 ± 184.3 µmol/L), reducing calcium accumulation in the aortic wall. Citrate-acidified bicarbonate dialysis reduces plasmatic calcium and pH variations during dialysis session (Δ[Ca
2+
] = −0.019 ± 0.089; ΔpH = 0.098 ± 0.043) respect to acetate-acidified bicarbonate dialysis (Δ[Ca
2+
] = 0.115 ± 0.118; ΔpH = 0.171 ± 0.078). To our knowledge, our study is the first to show that citrate protects against calcium accumulation in rat aortic walls
ex vivo
. Therefore, citrate-acidified bicarbonate dialysis may be an alternative approach to reduce calcification in hemodialysis patients without additional cost.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-47934-7</identifier><identifier>PMID: 31388059</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/106 ; 13/56 ; 631/443/592/2193 ; 631/443/7 ; Accumulation ; Acetates ; Acetic acid ; Animals ; Aorta ; Bicarbonates ; Calcification ; Calcification (ectopic) ; Calcium ; Citrates ; Citric acid ; Dialysis ; Dialysis Solutions - adverse effects ; Dialysis Solutions - chemistry ; Hemodialysis ; Heparin ; Humanities and Social Sciences ; Humans ; Male ; multidisciplinary ; pH effects ; Radioactive tracers ; Radioactivity ; Rats ; Rats, Sprague-Dawley ; Renal Dialysis - adverse effects ; Science ; Science (multidisciplinary) ; Urine ; Vascular Calcification - chemically induced ; Vascular Calcification - prevention & control</subject><ispartof>Scientific reports, 2019-08, Vol.9 (1), p.11374-7, Article 11374</ispartof><rights>The Author(s) 2019</rights><rights>2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-7682755446080f5e657b0ed48aa76369fb0700d3db11a4b37c37ca77db8e7e383</citedby><cites>FETCH-LOGICAL-c540t-7682755446080f5e657b0ed48aa76369fb0700d3db11a4b37c37ca77db8e7e383</cites><orcidid>0000-0002-1680-552X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6684644/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6684644/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31388059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Villa-Bellosta, Ricardo</creatorcontrib><creatorcontrib>Hernández-Martínez, Eduardo</creatorcontrib><creatorcontrib>Mérida-Herrero, Eva</creatorcontrib><creatorcontrib>González-Parra, Emilio</creatorcontrib><title>Impact of acetate- or citrate-acidified bicarbonate dialysate on ex vivo aorta wall calcification</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Vascular calcification is highly prevalent in patients with chronic hemodialysis. Increased acetatemia during hemodialysis sessions using acetate-acidified bicarbonate has also been associated with several abnormalities, By contrast, these abnormalities were not induced by citrate-acidified bicarbonate dialysis. Moreover, citrate is biocompatible alternative to acetate in dialysis fluid. However, the effects of citrate on vascular calcification during hemodialysis had not been studied in detail. This study analyzed herein the effects of acetate- or citrate-acidified bicarbonate dialysis on vascular calcification. Citrate has been shown to inhibit calcification in urine in hemodialysis patients. Therefore, our hypothesis is that citrate-acidified bicarbonate dialysis could reduce vascular calcification. Blood samples before and after hemodialysis from patients on acetate- or citrate-acidified bicarbonate dialysis were collected in heparin-containing tubes (n = 35 and n = 25 respectively). To explore the effect of pre- and post-dialysis plasmatic bicarbonate and citrate on vascular calcification, rats aortic rings cultured
ex vivo
in Minimum Essential Medium containing 0.1% FBS and 45-calcium as radiotracer were used (n = 24). After 7 days of incubation aortic rings were dried, weighed and radioactivity was measured via liquid scintillation counting. Bicarbonate levels increase calcium accumulation in rat aortic wall in a dose-response manner (pH = 7.4). Moreover, citrate prevents calcium accumulation, with a mean inhibitor concentration (IC
50
) value of 733 µmol/L. During acetate-acidified bicarbonate dialysis, bicarbonate and citrate levels in plasma increase (22.29 ± 3.59 versus 28.63 ± 3.56 mmol/L; p < 0.001) and decrease (133.3 ± 53.6 versus 87.49 ± 32.3 µmol/L, p < 0.001), respectively. These changes in pos-hemodialysis plasma significantly (p < 0.001) alter calcium accumulation in the aortic wall (38.9% higher). Moreover, citrate-acidified bicarbonate dialysis increases post-hemodialysis citrate levels 5-fold (145 ± 79.8 versus 771.6 ± 184.3 µmol/L), reducing calcium accumulation in the aortic wall. Citrate-acidified bicarbonate dialysis reduces plasmatic calcium and pH variations during dialysis session (Δ[Ca
2+
] = −0.019 ± 0.089; ΔpH = 0.098 ± 0.043) respect to acetate-acidified bicarbonate dialysis (Δ[Ca
2+
] = 0.115 ± 0.118; ΔpH = 0.171 ± 0.078). To our knowledge, our study is the first to show that citrate protects against calcium accumulation in rat aortic walls
ex vivo
. Therefore, citrate-acidified bicarbonate dialysis may be an alternative approach to reduce calcification in hemodialysis patients without additional cost.</description><subject>13</subject><subject>13/106</subject><subject>13/56</subject><subject>631/443/592/2193</subject><subject>631/443/7</subject><subject>Accumulation</subject><subject>Acetates</subject><subject>Acetic acid</subject><subject>Animals</subject><subject>Aorta</subject><subject>Bicarbonates</subject><subject>Calcification</subject><subject>Calcification (ectopic)</subject><subject>Calcium</subject><subject>Citrates</subject><subject>Citric acid</subject><subject>Dialysis</subject><subject>Dialysis Solutions - adverse effects</subject><subject>Dialysis Solutions - chemistry</subject><subject>Hemodialysis</subject><subject>Heparin</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Male</subject><subject>multidisciplinary</subject><subject>pH effects</subject><subject>Radioactive tracers</subject><subject>Radioactivity</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Renal Dialysis - adverse effects</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Urine</subject><subject>Vascular Calcification - chemically induced</subject><subject>Vascular Calcification - prevention & control</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9UctKBDEQDKKoqD_gQQKeR_NO5iKI-FgQvOg59GQya2R2siazq_69WdfnxRBI0V1V3aEQOqTkhBJuTrOgsjYVoXUldM1FpTfQLiNCVowztvkL76CDnJ9IOZLVgtbbaIdTbgyR9S6CyWwObsSxw-D8CKOvcEzYhTGtMLjQhi74FjfBQWriUKq4DdC_5RWKA_aveBmWEUNMI-AX6HvsoHdF5WAMcdhHWx302R98vnvo4ery_uKmur27nlyc31ZOCjJWWhmmpRRCEUM66ZXUDfGtMABacVV3DdGEtLxtKAXRcO3KBa3bxnjtueF76GztO180M986P5Qv9HaewgzSm40Q7N_OEB7tNC6tUkYoIYrB8adBis8Ln0f7FBdpKDtbxpTRNaNyxWJrlksx5-S77wmU2FUydp2MLcnYj2SsLqKj37t9S75yKAS-JuTSGqY-_cz-x_YdrXuaSQ</recordid><startdate>20190806</startdate><enddate>20190806</enddate><creator>Villa-Bellosta, Ricardo</creator><creator>Hernández-Martínez, Eduardo</creator><creator>Mérida-Herrero, Eva</creator><creator>González-Parra, Emilio</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1680-552X</orcidid></search><sort><creationdate>20190806</creationdate><title>Impact of acetate- or citrate-acidified bicarbonate dialysate on ex vivo aorta wall calcification</title><author>Villa-Bellosta, Ricardo ; Hernández-Martínez, Eduardo ; Mérida-Herrero, Eva ; González-Parra, Emilio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-7682755446080f5e657b0ed48aa76369fb0700d3db11a4b37c37ca77db8e7e383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>13</topic><topic>13/106</topic><topic>13/56</topic><topic>631/443/592/2193</topic><topic>631/443/7</topic><topic>Accumulation</topic><topic>Acetates</topic><topic>Acetic acid</topic><topic>Animals</topic><topic>Aorta</topic><topic>Bicarbonates</topic><topic>Calcification</topic><topic>Calcification (ectopic)</topic><topic>Calcium</topic><topic>Citrates</topic><topic>Citric acid</topic><topic>Dialysis</topic><topic>Dialysis Solutions - adverse effects</topic><topic>Dialysis Solutions - chemistry</topic><topic>Hemodialysis</topic><topic>Heparin</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Male</topic><topic>multidisciplinary</topic><topic>pH effects</topic><topic>Radioactive tracers</topic><topic>Radioactivity</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Renal Dialysis - adverse effects</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Urine</topic><topic>Vascular Calcification - chemically induced</topic><topic>Vascular Calcification - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Villa-Bellosta, Ricardo</creatorcontrib><creatorcontrib>Hernández-Martínez, Eduardo</creatorcontrib><creatorcontrib>Mérida-Herrero, Eva</creatorcontrib><creatorcontrib>González-Parra, Emilio</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Villa-Bellosta, Ricardo</au><au>Hernández-Martínez, Eduardo</au><au>Mérida-Herrero, Eva</au><au>González-Parra, Emilio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of acetate- or citrate-acidified bicarbonate dialysate on ex vivo aorta wall calcification</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-08-06</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>11374</spage><epage>7</epage><pages>11374-7</pages><artnum>11374</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Vascular calcification is highly prevalent in patients with chronic hemodialysis. Increased acetatemia during hemodialysis sessions using acetate-acidified bicarbonate has also been associated with several abnormalities, By contrast, these abnormalities were not induced by citrate-acidified bicarbonate dialysis. Moreover, citrate is biocompatible alternative to acetate in dialysis fluid. However, the effects of citrate on vascular calcification during hemodialysis had not been studied in detail. This study analyzed herein the effects of acetate- or citrate-acidified bicarbonate dialysis on vascular calcification. Citrate has been shown to inhibit calcification in urine in hemodialysis patients. Therefore, our hypothesis is that citrate-acidified bicarbonate dialysis could reduce vascular calcification. Blood samples before and after hemodialysis from patients on acetate- or citrate-acidified bicarbonate dialysis were collected in heparin-containing tubes (n = 35 and n = 25 respectively). To explore the effect of pre- and post-dialysis plasmatic bicarbonate and citrate on vascular calcification, rats aortic rings cultured
ex vivo
in Minimum Essential Medium containing 0.1% FBS and 45-calcium as radiotracer were used (n = 24). After 7 days of incubation aortic rings were dried, weighed and radioactivity was measured via liquid scintillation counting. Bicarbonate levels increase calcium accumulation in rat aortic wall in a dose-response manner (pH = 7.4). Moreover, citrate prevents calcium accumulation, with a mean inhibitor concentration (IC
50
) value of 733 µmol/L. During acetate-acidified bicarbonate dialysis, bicarbonate and citrate levels in plasma increase (22.29 ± 3.59 versus 28.63 ± 3.56 mmol/L; p < 0.001) and decrease (133.3 ± 53.6 versus 87.49 ± 32.3 µmol/L, p < 0.001), respectively. These changes in pos-hemodialysis plasma significantly (p < 0.001) alter calcium accumulation in the aortic wall (38.9% higher). Moreover, citrate-acidified bicarbonate dialysis increases post-hemodialysis citrate levels 5-fold (145 ± 79.8 versus 771.6 ± 184.3 µmol/L), reducing calcium accumulation in the aortic wall. Citrate-acidified bicarbonate dialysis reduces plasmatic calcium and pH variations during dialysis session (Δ[Ca
2+
] = −0.019 ± 0.089; ΔpH = 0.098 ± 0.043) respect to acetate-acidified bicarbonate dialysis (Δ[Ca
2+
] = 0.115 ± 0.118; ΔpH = 0.171 ± 0.078). To our knowledge, our study is the first to show that citrate protects against calcium accumulation in rat aortic walls
ex vivo
. Therefore, citrate-acidified bicarbonate dialysis may be an alternative approach to reduce calcification in hemodialysis patients without additional cost.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31388059</pmid><doi>10.1038/s41598-019-47934-7</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-1680-552X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Nature Free; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 13 13/106 13/56 631/443/592/2193 631/443/7 Accumulation Acetates Acetic acid Animals Aorta Bicarbonates Calcification Calcification (ectopic) Calcium Citrates Citric acid Dialysis Dialysis Solutions - adverse effects Dialysis Solutions - chemistry Hemodialysis Heparin Humanities and Social Sciences Humans Male multidisciplinary pH effects Radioactive tracers Radioactivity Rats Rats, Sprague-Dawley Renal Dialysis - adverse effects Science Science (multidisciplinary) Urine Vascular Calcification - chemically induced Vascular Calcification - prevention & control |
| title | Impact of acetate- or citrate-acidified bicarbonate dialysate on ex vivo aorta wall calcification |
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