The Functional Requirement for CD69 in Establishment of Resident Memory CD8 + T Cells Varies with Tissue Location

Recent studies have characterized populations of memory CD8 T cells that do not recirculate through the blood but are, instead, retained in nonlymphoid tissues. Such CD8 tissue resident memory T cells (T ) are critical for pathogen control at barrier sites. Identifying T and defining the basis for t...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Journal of immunology (1950) 2019-08, Vol.203 (4), p.946-955
Hauptverfasser: Walsh, Daniel A, Borges da Silva, Henrique, Beura, Lalit K, Peng, Changwei, Hamilton, Sara E, Masopust, David, Jameson, Stephen C
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 955
container_issue 4
container_start_page 946
container_title The Journal of immunology (1950)
container_volume 203
creator Walsh, Daniel A
Borges da Silva, Henrique
Beura, Lalit K
Peng, Changwei
Hamilton, Sara E
Masopust, David
Jameson, Stephen C
description Recent studies have characterized populations of memory CD8 T cells that do not recirculate through the blood but are, instead, retained in nonlymphoid tissues. Such CD8 tissue resident memory T cells (T ) are critical for pathogen control at barrier sites. Identifying T and defining the basis for their tissue residency is therefore of considerable importance for understanding protective immunity and improved vaccine design. Expression of the molecule CD69 is widely used as a definitive marker for T , yet it is unclear whether CD69 is universally required for producing or retaining T Using multiple mouse models of acute immunization, we found that the functional requirement for CD69 was highly variable, depending on the tissue examined, playing no detectable role in generation of T at some sites (such as the small intestine), whereas CD69 was critical for establishing resident cells in the kidney. Likewise, forced expression of CD69 (but not expression of a CD69 mutant unable to bind the egress factor S1PR1) promoted CD8 T generation in the kidney but not in other tissues. Our findings indicate that the functional relevance of CD69 in generation and maintenance of CD8 T varies considerably, chiefly dependent on the specific nonlymphoid tissue studied. Together with previous reports that suggest uncoupling of CD69 expression and tissue residency, these findings prompt caution in reliance on CD69 expression as a consistent marker of CD8 T .
doi_str_mv 10.4049/jimmunol.1900052
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6684481</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2248372723</sourcerecordid><originalsourceid>FETCH-LOGICAL-c396t-9e13843f3f3069e0769fd8745683ea7ae6d2971c45f515a1a016367c1caa970b3</originalsourceid><addsrcrecordid>eNpVkd9LHDEQx4NU6tX23aeSx4KsnfzYZPMiyFXbwokgp68hl5vtRXY3XrJb8b9vrp7SMg8zMJ_5zgxfQk4YnEmQ5utD6PtpiN0ZMwBQ8wMyY3UNlVKg3pEZAOcV00ofkQ85PxREAZfvyZFgXAowfEa2yw3Sq2nwY4iD6-gtbqeQsMdhpG1MdP5NGRoGeplHt-pC3vztxLaAOax39TX2MT0XsKGndEnn2HWZ3rsUMNOnMG7oMuQ8IV1E73ZLPpLD1nUZP-3zMbm7ulzOf1SLm-8_5xeLygujxsogE40UbQlQBkEr064bLWvVCHTaoVpzo5mXdVuz2jEHTAmlPfPOGQ0rcUzOX3Qfp1WPa19uTa6zjyn0Lj3b6IL9vzOEjf0Vf1ulGikbVgS-7AVS3E6YR9uH7Mt7bsA4Zcu5bITmmouCwgvqU8w5Yfu2hoHdOWVfnbJ7p8rI53_Pext4tUb8ASOFkTQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2248372723</pqid></control><display><type>article</type><title>The Functional Requirement for CD69 in Establishment of Resident Memory CD8 + T Cells Varies with Tissue Location</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Walsh, Daniel A ; Borges da Silva, Henrique ; Beura, Lalit K ; Peng, Changwei ; Hamilton, Sara E ; Masopust, David ; Jameson, Stephen C</creator><creatorcontrib>Walsh, Daniel A ; Borges da Silva, Henrique ; Beura, Lalit K ; Peng, Changwei ; Hamilton, Sara E ; Masopust, David ; Jameson, Stephen C</creatorcontrib><description>Recent studies have characterized populations of memory CD8 T cells that do not recirculate through the blood but are, instead, retained in nonlymphoid tissues. Such CD8 tissue resident memory T cells (T ) are critical for pathogen control at barrier sites. Identifying T and defining the basis for their tissue residency is therefore of considerable importance for understanding protective immunity and improved vaccine design. Expression of the molecule CD69 is widely used as a definitive marker for T , yet it is unclear whether CD69 is universally required for producing or retaining T Using multiple mouse models of acute immunization, we found that the functional requirement for CD69 was highly variable, depending on the tissue examined, playing no detectable role in generation of T at some sites (such as the small intestine), whereas CD69 was critical for establishing resident cells in the kidney. Likewise, forced expression of CD69 (but not expression of a CD69 mutant unable to bind the egress factor S1PR1) promoted CD8 T generation in the kidney but not in other tissues. Our findings indicate that the functional relevance of CD69 in generation and maintenance of CD8 T varies considerably, chiefly dependent on the specific nonlymphoid tissue studied. Together with previous reports that suggest uncoupling of CD69 expression and tissue residency, these findings prompt caution in reliance on CD69 expression as a consistent marker of CD8 T .</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.1900052</identifier><identifier>PMID: 31243092</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Antigens, CD - immunology ; Antigens, Differentiation, T-Lymphocyte - immunology ; CD8-Positive T-Lymphocytes - immunology ; Immunologic Memory - immunology ; Lectins, C-Type - immunology ; Mice ; T-Lymphocyte Subsets - immunology</subject><ispartof>The Journal of immunology (1950), 2019-08, Vol.203 (4), p.946-955</ispartof><rights>Copyright © 2019 by The American Association of Immunologists, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-9e13843f3f3069e0769fd8745683ea7ae6d2971c45f515a1a016367c1caa970b3</citedby><cites>FETCH-LOGICAL-c396t-9e13843f3f3069e0769fd8745683ea7ae6d2971c45f515a1a016367c1caa970b3</cites><orcidid>0000-0001-9137-1146 ; 0000-0002-0710-9573 ; 0000-0002-5021-6200 ; 0000-0002-8597-3949</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31243092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Walsh, Daniel A</creatorcontrib><creatorcontrib>Borges da Silva, Henrique</creatorcontrib><creatorcontrib>Beura, Lalit K</creatorcontrib><creatorcontrib>Peng, Changwei</creatorcontrib><creatorcontrib>Hamilton, Sara E</creatorcontrib><creatorcontrib>Masopust, David</creatorcontrib><creatorcontrib>Jameson, Stephen C</creatorcontrib><title>The Functional Requirement for CD69 in Establishment of Resident Memory CD8 + T Cells Varies with Tissue Location</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>Recent studies have characterized populations of memory CD8 T cells that do not recirculate through the blood but are, instead, retained in nonlymphoid tissues. Such CD8 tissue resident memory T cells (T ) are critical for pathogen control at barrier sites. Identifying T and defining the basis for their tissue residency is therefore of considerable importance for understanding protective immunity and improved vaccine design. Expression of the molecule CD69 is widely used as a definitive marker for T , yet it is unclear whether CD69 is universally required for producing or retaining T Using multiple mouse models of acute immunization, we found that the functional requirement for CD69 was highly variable, depending on the tissue examined, playing no detectable role in generation of T at some sites (such as the small intestine), whereas CD69 was critical for establishing resident cells in the kidney. Likewise, forced expression of CD69 (but not expression of a CD69 mutant unable to bind the egress factor S1PR1) promoted CD8 T generation in the kidney but not in other tissues. Our findings indicate that the functional relevance of CD69 in generation and maintenance of CD8 T varies considerably, chiefly dependent on the specific nonlymphoid tissue studied. Together with previous reports that suggest uncoupling of CD69 expression and tissue residency, these findings prompt caution in reliance on CD69 expression as a consistent marker of CD8 T .</description><subject>Animals</subject><subject>Antigens, CD - immunology</subject><subject>Antigens, Differentiation, T-Lymphocyte - immunology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Immunologic Memory - immunology</subject><subject>Lectins, C-Type - immunology</subject><subject>Mice</subject><subject>T-Lymphocyte Subsets - immunology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkd9LHDEQx4NU6tX23aeSx4KsnfzYZPMiyFXbwokgp68hl5vtRXY3XrJb8b9vrp7SMg8zMJ_5zgxfQk4YnEmQ5utD6PtpiN0ZMwBQ8wMyY3UNlVKg3pEZAOcV00ofkQ85PxREAZfvyZFgXAowfEa2yw3Sq2nwY4iD6-gtbqeQsMdhpG1MdP5NGRoGeplHt-pC3vztxLaAOax39TX2MT0XsKGndEnn2HWZ3rsUMNOnMG7oMuQ8IV1E73ZLPpLD1nUZP-3zMbm7ulzOf1SLm-8_5xeLygujxsogE40UbQlQBkEr064bLWvVCHTaoVpzo5mXdVuz2jEHTAmlPfPOGQ0rcUzOX3Qfp1WPa19uTa6zjyn0Lj3b6IL9vzOEjf0Vf1ulGikbVgS-7AVS3E6YR9uH7Mt7bsA4Zcu5bITmmouCwgvqU8w5Yfu2hoHdOWVfnbJ7p8rI53_Pext4tUb8ASOFkTQ</recordid><startdate>20190815</startdate><enddate>20190815</enddate><creator>Walsh, Daniel A</creator><creator>Borges da Silva, Henrique</creator><creator>Beura, Lalit K</creator><creator>Peng, Changwei</creator><creator>Hamilton, Sara E</creator><creator>Masopust, David</creator><creator>Jameson, Stephen C</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9137-1146</orcidid><orcidid>https://orcid.org/0000-0002-0710-9573</orcidid><orcidid>https://orcid.org/0000-0002-5021-6200</orcidid><orcidid>https://orcid.org/0000-0002-8597-3949</orcidid></search><sort><creationdate>20190815</creationdate><title>The Functional Requirement for CD69 in Establishment of Resident Memory CD8 + T Cells Varies with Tissue Location</title><author>Walsh, Daniel A ; Borges da Silva, Henrique ; Beura, Lalit K ; Peng, Changwei ; Hamilton, Sara E ; Masopust, David ; Jameson, Stephen C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-9e13843f3f3069e0769fd8745683ea7ae6d2971c45f515a1a016367c1caa970b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Antigens, CD - immunology</topic><topic>Antigens, Differentiation, T-Lymphocyte - immunology</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Immunologic Memory - immunology</topic><topic>Lectins, C-Type - immunology</topic><topic>Mice</topic><topic>T-Lymphocyte Subsets - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Walsh, Daniel A</creatorcontrib><creatorcontrib>Borges da Silva, Henrique</creatorcontrib><creatorcontrib>Beura, Lalit K</creatorcontrib><creatorcontrib>Peng, Changwei</creatorcontrib><creatorcontrib>Hamilton, Sara E</creatorcontrib><creatorcontrib>Masopust, David</creatorcontrib><creatorcontrib>Jameson, Stephen C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Walsh, Daniel A</au><au>Borges da Silva, Henrique</au><au>Beura, Lalit K</au><au>Peng, Changwei</au><au>Hamilton, Sara E</au><au>Masopust, David</au><au>Jameson, Stephen C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Functional Requirement for CD69 in Establishment of Resident Memory CD8 + T Cells Varies with Tissue Location</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>2019-08-15</date><risdate>2019</risdate><volume>203</volume><issue>4</issue><spage>946</spage><epage>955</epage><pages>946-955</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>Recent studies have characterized populations of memory CD8 T cells that do not recirculate through the blood but are, instead, retained in nonlymphoid tissues. Such CD8 tissue resident memory T cells (T ) are critical for pathogen control at barrier sites. Identifying T and defining the basis for their tissue residency is therefore of considerable importance for understanding protective immunity and improved vaccine design. Expression of the molecule CD69 is widely used as a definitive marker for T , yet it is unclear whether CD69 is universally required for producing or retaining T Using multiple mouse models of acute immunization, we found that the functional requirement for CD69 was highly variable, depending on the tissue examined, playing no detectable role in generation of T at some sites (such as the small intestine), whereas CD69 was critical for establishing resident cells in the kidney. Likewise, forced expression of CD69 (but not expression of a CD69 mutant unable to bind the egress factor S1PR1) promoted CD8 T generation in the kidney but not in other tissues. Our findings indicate that the functional relevance of CD69 in generation and maintenance of CD8 T varies considerably, chiefly dependent on the specific nonlymphoid tissue studied. Together with previous reports that suggest uncoupling of CD69 expression and tissue residency, these findings prompt caution in reliance on CD69 expression as a consistent marker of CD8 T .</abstract><cop>United States</cop><pmid>31243092</pmid><doi>10.4049/jimmunol.1900052</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-9137-1146</orcidid><orcidid>https://orcid.org/0000-0002-0710-9573</orcidid><orcidid>https://orcid.org/0000-0002-5021-6200</orcidid><orcidid>https://orcid.org/0000-0002-8597-3949</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0022-1767
ispartof The Journal of immunology (1950), 2019-08, Vol.203 (4), p.946-955
issn 0022-1767
1550-6606
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6684481
source MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Animals
Antigens, CD - immunology
Antigens, Differentiation, T-Lymphocyte - immunology
CD8-Positive T-Lymphocytes - immunology
Immunologic Memory - immunology
Lectins, C-Type - immunology
Mice
T-Lymphocyte Subsets - immunology
title The Functional Requirement for CD69 in Establishment of Resident Memory CD8 + T Cells Varies with Tissue Location
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T01%3A22%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Functional%20Requirement%20for%20CD69%20in%20Establishment%20of%20Resident%20Memory%20CD8%20+%20T%20Cells%20Varies%20with%20Tissue%20Location&rft.jtitle=The%20Journal%20of%20immunology%20(1950)&rft.au=Walsh,%20Daniel%20A&rft.date=2019-08-15&rft.volume=203&rft.issue=4&rft.spage=946&rft.epage=955&rft.pages=946-955&rft.issn=0022-1767&rft.eissn=1550-6606&rft_id=info:doi/10.4049/jimmunol.1900052&rft_dat=%3Cproquest_pubme%3E2248372723%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2248372723&rft_id=info:pmid/31243092&rfr_iscdi=true