Antiproliferative and Antitumour Effect of Nongenotoxic Silver Nanoparticles on Melanoma Models
During the last 3 decades, there has been a slow advance to obtain new treatments for malignant melanoma that improve patient survival. In this work, we present a systematic study focused on the antiproliferative and antitumour effect of AgNPs. These nanoparticles are fully characterized, are coated...
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| Veröffentlicht in: | Oxidative medicine and cellular longevity 2019, Vol.2019 (2019), p.1-12 |
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| creator | Bogdanchikova, Nina Toledano-Magaña, Yanis Pestryakov, Alexey Gómez, Claudia Torres-Bugarín, Olivia García-Ramos, Juan C. Girón-Vázquez, Nayeli G. Valenzuela-Salas, Lucía M. Villarreal-Gómez, Luis J. |
| description | During the last 3 decades, there has been a slow advance to obtain new treatments for malignant melanoma that improve patient survival. In this work, we present a systematic study focused on the antiproliferative and antitumour effect of AgNPs. These nanoparticles are fully characterized, are coated with polyvinylpyrrolidone (PVP), and have an average size of 35±15 nm and a metallic silver content of 1.2% wt. Main changes on cell viability, induction of apoptosis and necrosis, and ROS generation were found on B16-F10 cells after six hours of exposure to AgNPs (IC50=4.2 μg/mL) or Cisplatin (IC50=2.0 μg/mL). Despite the similar response for both AgNPs and Cisplatin on antiproliferative potency (cellular viability of 53.95±1.88 and 53.62±1.04) and ROS production (20.27±1.09% and 19.50±0.35%), significantly different cell death pathways were triggered. While AgNPs induce only apoptosis (45.98±1.88%), Cisplatin induces apoptosis and necrosis at the same rate (22.31±1.72% and 24.07±1.10%, respectively). In addition to their antiproliferative activity, in vivo experiments showed that treatments of 3, 6, and 12 mg/kg of AgNPs elicit a survival rate almost 4 times higher (P |
| doi_str_mv | 10.1155/2019/4528241 |
| format | Article |
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In this work, we present a systematic study focused on the antiproliferative and antitumour effect of AgNPs. These nanoparticles are fully characterized, are coated with polyvinylpyrrolidone (PVP), and have an average size of 35±15 nm and a metallic silver content of 1.2% wt. Main changes on cell viability, induction of apoptosis and necrosis, and ROS generation were found on B16-F10 cells after six hours of exposure to AgNPs (IC50=4.2 μg/mL) or Cisplatin (IC50=2.0 μg/mL). Despite the similar response for both AgNPs and Cisplatin on antiproliferative potency (cellular viability of 53.95±1.88 and 53.62±1.04) and ROS production (20.27±1.09% and 19.50±0.35%), significantly different cell death pathways were triggered. While AgNPs induce only apoptosis (45.98±1.88%), Cisplatin induces apoptosis and necrosis at the same rate (22.31±1.72% and 24.07±1.10%, respectively). In addition to their antiproliferative activity, in vivo experiments showed that treatments of 3, 6, and 12 mg/kg of AgNPs elicit a survival rate almost 4 times higher (P<0.05) compared with the survival rate obtained with Cisplatin (2 mg/kg). Furthermore, the survivor mice treated with AgNPs do not show genotoxic damage determined by micronuclei frequency quantification on peripheral blood cells. These results exhibit the remarkable antitumour activity of a nongenotoxic AgNP formulation and constitute the first advance toward the application of these AgNPs for melanoma treatment, which could considerably reduce adverse effects provoked by currently applied chemotherapeutics.</description><identifier>ISSN: 1942-0900</identifier><identifier>EISSN: 1942-0994</identifier><identifier>DOI: 10.1155/2019/4528241</identifier><identifier>PMID: 31428226</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Animals ; Apoptosis ; Apoptosis - drug effects ; Autophagy ; Cancer ; Cancer therapies ; Care and treatment ; Cell growth ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cisplatin ; Cisplatin - therapeutic use ; Cytotoxicity ; DNA Damage - drug effects ; Erythrocytes - cytology ; Erythrocytes - drug effects ; Erythrocytes - metabolism ; Hematology ; Iron ; Kaplan-Meier Estimate ; Male ; Melanoma ; Melanoma, Experimental - drug therapy ; Melanoma, Experimental - mortality ; Melanoma, Experimental - pathology ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - therapeutic use ; Metal Nanoparticles - toxicity ; Mice ; Mice, Inbred C57BL ; Nanomaterials ; Nanoparticles ; Povidone ; Reactive Oxygen Species - metabolism ; Silver ; Silver - chemistry ; Skin cancer ; Toxicology</subject><ispartof>Oxidative medicine and cellular longevity, 2019, Vol.2019 (2019), p.1-12</ispartof><rights>Copyright © 2019 Lucía M. Valenzuela-Salas et al.</rights><rights>COPYRIGHT 2019 John Wiley & Sons, Inc.</rights><rights>Copyright © 2019 Lucía M. Valenzuela-Salas et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2019 Lucía M. Valenzuela-Salas et al. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-f16ec3f0f46dd3df5a9f80ef304cdff7f628956d5d0e9439c237193b7bd203613</citedby><cites>FETCH-LOGICAL-c499t-f16ec3f0f46dd3df5a9f80ef304cdff7f628956d5d0e9439c237193b7bd203613</cites><orcidid>0000-0002-2595-7939 ; 0000-0002-4666-1408</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683800/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683800/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31428226$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kaushik, Nagendra K.</contributor><contributor>Nagendra K Kaushik</contributor><creatorcontrib>Bogdanchikova, Nina</creatorcontrib><creatorcontrib>Toledano-Magaña, Yanis</creatorcontrib><creatorcontrib>Pestryakov, Alexey</creatorcontrib><creatorcontrib>Gómez, Claudia</creatorcontrib><creatorcontrib>Torres-Bugarín, Olivia</creatorcontrib><creatorcontrib>García-Ramos, Juan C.</creatorcontrib><creatorcontrib>Girón-Vázquez, Nayeli G.</creatorcontrib><creatorcontrib>Valenzuela-Salas, Lucía M.</creatorcontrib><creatorcontrib>Villarreal-Gómez, Luis J.</creatorcontrib><title>Antiproliferative and Antitumour Effect of Nongenotoxic Silver Nanoparticles on Melanoma Models</title><title>Oxidative medicine and cellular longevity</title><addtitle>Oxid Med Cell Longev</addtitle><description>During the last 3 decades, there has been a slow advance to obtain new treatments for malignant melanoma that improve patient survival. In this work, we present a systematic study focused on the antiproliferative and antitumour effect of AgNPs. These nanoparticles are fully characterized, are coated with polyvinylpyrrolidone (PVP), and have an average size of 35±15 nm and a metallic silver content of 1.2% wt. Main changes on cell viability, induction of apoptosis and necrosis, and ROS generation were found on B16-F10 cells after six hours of exposure to AgNPs (IC50=4.2 μg/mL) or Cisplatin (IC50=2.0 μg/mL). Despite the similar response for both AgNPs and Cisplatin on antiproliferative potency (cellular viability of 53.95±1.88 and 53.62±1.04) and ROS production (20.27±1.09% and 19.50±0.35%), significantly different cell death pathways were triggered. While AgNPs induce only apoptosis (45.98±1.88%), Cisplatin induces apoptosis and necrosis at the same rate (22.31±1.72% and 24.07±1.10%, respectively). In addition to their antiproliferative activity, in vivo experiments showed that treatments of 3, 6, and 12 mg/kg of AgNPs elicit a survival rate almost 4 times higher (P<0.05) compared with the survival rate obtained with Cisplatin (2 mg/kg). Furthermore, the survivor mice treated with AgNPs do not show genotoxic damage determined by micronuclei frequency quantification on peripheral blood cells. These results exhibit the remarkable antitumour activity of a nongenotoxic AgNP formulation and constitute the first advance toward the application of these AgNPs for melanoma treatment, which could considerably reduce adverse effects provoked by currently applied chemotherapeutics.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Autophagy</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Care and treatment</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cisplatin</subject><subject>Cisplatin - therapeutic use</subject><subject>Cytotoxicity</subject><subject>DNA Damage - drug effects</subject><subject>Erythrocytes - cytology</subject><subject>Erythrocytes - drug effects</subject><subject>Erythrocytes - metabolism</subject><subject>Hematology</subject><subject>Iron</subject><subject>Kaplan-Meier Estimate</subject><subject>Male</subject><subject>Melanoma</subject><subject>Melanoma, Experimental - drug therapy</subject><subject>Melanoma, Experimental - mortality</subject><subject>Melanoma, Experimental - pathology</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metal Nanoparticles - therapeutic use</subject><subject>Metal Nanoparticles - toxicity</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Povidone</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Silver</subject><subject>Silver - chemistry</subject><subject>Skin cancer</subject><subject>Toxicology</subject><issn>1942-0900</issn><issn>1942-0994</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkc1vEzEQxS0EoqVw44wscSyh44911hekqCofUlsOwNly7HHqatcO9ibAf4-jhBRunGY089PTm3mEvGTwlrGuu-DA9IXseM8le0ROmZZ8BlrLx8ce4IQ8q_UeQIkGPSUngsnGc3VKzCJNcV3yEAMWO8UtUps83U2nzZg3hV6FgG6iOdDbnFaY8pR_Rke_xGGLhd7alNe2TNENWGlO9AaHNhotvckeh_qcPAl2qPjiUM_It_dXXy8_zq4_f_h0ubieOan1NAtMoRMBglTeCx86q0MPGARI50OYB8V73SnfeUAthXZczJkWy_nScxCKiTPybq-73ixH9A7TVOxg1iWOtvwy2Ubz7ybFO7PKW6NUL3qAJvD6IFDy9w3Wydy361PzbNqjeq4YCP5AreyAJqaQm5gbY3VmoYAB73veNerNnnIl11owHH0wMLvQzC40cwit4a_-9n6E_6TUgPM9cBeTtz_if8phYzDYB3r3qnbqbzqHqWM</recordid><startdate>2019</startdate><enddate>2019</enddate><creator>Bogdanchikova, Nina</creator><creator>Toledano-Magaña, Yanis</creator><creator>Pestryakov, Alexey</creator><creator>Gómez, Claudia</creator><creator>Torres-Bugarín, Olivia</creator><creator>García-Ramos, Juan C.</creator><creator>Girón-Vázquez, Nayeli G.</creator><creator>Valenzuela-Salas, Lucía M.</creator><creator>Villarreal-Gómez, Luis J.</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2595-7939</orcidid><orcidid>https://orcid.org/0000-0002-4666-1408</orcidid></search><sort><creationdate>2019</creationdate><title>Antiproliferative and Antitumour Effect of Nongenotoxic Silver Nanoparticles on Melanoma Models</title><author>Bogdanchikova, Nina ; Toledano-Magaña, Yanis ; Pestryakov, Alexey ; Gómez, Claudia ; Torres-Bugarín, Olivia ; García-Ramos, Juan C. ; Girón-Vázquez, Nayeli G. ; Valenzuela-Salas, Lucía M. ; Villarreal-Gómez, Luis J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-f16ec3f0f46dd3df5a9f80ef304cdff7f628956d5d0e9439c237193b7bd203613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Autophagy</topic><topic>Cancer</topic><topic>Cancer therapies</topic><topic>Care and treatment</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Cisplatin</topic><topic>Cisplatin - therapeutic use</topic><topic>Cytotoxicity</topic><topic>DNA Damage - drug effects</topic><topic>Erythrocytes - cytology</topic><topic>Erythrocytes - drug effects</topic><topic>Erythrocytes - metabolism</topic><topic>Hematology</topic><topic>Iron</topic><topic>Kaplan-Meier Estimate</topic><topic>Male</topic><topic>Melanoma</topic><topic>Melanoma, Experimental - drug therapy</topic><topic>Melanoma, Experimental - mortality</topic><topic>Melanoma, Experimental - pathology</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Metal Nanoparticles - therapeutic use</topic><topic>Metal Nanoparticles - toxicity</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Povidone</topic><topic>Reactive Oxygen Species - 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In this work, we present a systematic study focused on the antiproliferative and antitumour effect of AgNPs. These nanoparticles are fully characterized, are coated with polyvinylpyrrolidone (PVP), and have an average size of 35±15 nm and a metallic silver content of 1.2% wt. Main changes on cell viability, induction of apoptosis and necrosis, and ROS generation were found on B16-F10 cells after six hours of exposure to AgNPs (IC50=4.2 μg/mL) or Cisplatin (IC50=2.0 μg/mL). Despite the similar response for both AgNPs and Cisplatin on antiproliferative potency (cellular viability of 53.95±1.88 and 53.62±1.04) and ROS production (20.27±1.09% and 19.50±0.35%), significantly different cell death pathways were triggered. While AgNPs induce only apoptosis (45.98±1.88%), Cisplatin induces apoptosis and necrosis at the same rate (22.31±1.72% and 24.07±1.10%, respectively). In addition to their antiproliferative activity, in vivo experiments showed that treatments of 3, 6, and 12 mg/kg of AgNPs elicit a survival rate almost 4 times higher (P<0.05) compared with the survival rate obtained with Cisplatin (2 mg/kg). Furthermore, the survivor mice treated with AgNPs do not show genotoxic damage determined by micronuclei frequency quantification on peripheral blood cells. These results exhibit the remarkable antitumour activity of a nongenotoxic AgNP formulation and constitute the first advance toward the application of these AgNPs for melanoma treatment, which could considerably reduce adverse effects provoked by currently applied chemotherapeutics.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>31428226</pmid><doi>10.1155/2019/4528241</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-2595-7939</orcidid><orcidid>https://orcid.org/0000-0002-4666-1408</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Oxidative medicine and cellular longevity, 2019, Vol.2019 (2019), p.1-12 |
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| source | MEDLINE; Wiley Online Library Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Animals Apoptosis Apoptosis - drug effects Autophagy Cancer Cancer therapies Care and treatment Cell growth Cell Line, Tumor Cell Proliferation - drug effects Cisplatin Cisplatin - therapeutic use Cytotoxicity DNA Damage - drug effects Erythrocytes - cytology Erythrocytes - drug effects Erythrocytes - metabolism Hematology Iron Kaplan-Meier Estimate Male Melanoma Melanoma, Experimental - drug therapy Melanoma, Experimental - mortality Melanoma, Experimental - pathology Metal Nanoparticles - chemistry Metal Nanoparticles - therapeutic use Metal Nanoparticles - toxicity Mice Mice, Inbred C57BL Nanomaterials Nanoparticles Povidone Reactive Oxygen Species - metabolism Silver Silver - chemistry Skin cancer Toxicology |
| title | Antiproliferative and Antitumour Effect of Nongenotoxic Silver Nanoparticles on Melanoma Models |
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