Complete genome and plasmid sequence of a novel Bacillus sp. BD59S, a parasporal protein synthesizing bacterium
Parasporal crystalline inclusion proteins of some Bacillus spp. are of paramount importance due to their insecticidal, nematocidal, and cancer cell killing capabilities. Here, we present a brief report of the complete genome sequence of Bacillus sp. BD59S, a bacterium that produced HeLa cell-killing...
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| Veröffentlicht in: | 3 Biotech 2019-09, Vol.9 (9), p.318-8, Article 318 |
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| creator | Hasan, Md. Mahmud Khan, Shakila Nargis Karim, Muhammad Manjurul Begum, Anowara Hoq, Md. Mozammel |
| description | Parasporal crystalline inclusion proteins of some
Bacillus
spp. are of paramount importance due to their insecticidal, nematocidal, and cancer cell killing capabilities. Here, we present a brief report of the complete genome sequence of
Bacillus
sp. BD59S, a bacterium that produced HeLa cell-killing parasporal crystalline inclusion proteins. From genome sequencing and assembly, we found that the bacterium has one circular chromosome and two large plasmids, pBTBD59S1 and pBTBD59S2. The size of the chromosome is 5283,933 bp with a 35.4% GC content, consisting of 5938 genes and 5550 protein-coding sequences (CDSs), 25 complete rRNAs (5S, 16S, 23S), 98 tRNAs, 5 ncRNAs, 260 pseudo-genes, and 356 subsystems. Complete plasmid sequence of pBTBD59S1 comprises a total size of 162,149 bp with 33.4% GC content, 192 CDSs, and 13 subsystems. The other plasmid pBTBD59S2, is 199,209 bp long with 32.9% GC content, 179 CDSs, and 11 subsystems. Analyses by NCBI microbial genome BLAST, phylogenetic genome tree, and BLAST ring image generator (BRIG) revealed that BD59S belongs to
Bacillus cereus
group, and is more close to
B. thuringiensis
. Further, the strain possesses 57.04 kDa and 54.42 kDa Cry protein-coding genes, which show significant similarities with cancer cell-killing parasporin proteins of
B. thuringiensis
strains. |
| doi_str_mv | 10.1007/s13205-019-1849-7 |
| format | Article |
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Bacillus
spp. are of paramount importance due to their insecticidal, nematocidal, and cancer cell killing capabilities. Here, we present a brief report of the complete genome sequence of
Bacillus
sp. BD59S, a bacterium that produced HeLa cell-killing parasporal crystalline inclusion proteins. From genome sequencing and assembly, we found that the bacterium has one circular chromosome and two large plasmids, pBTBD59S1 and pBTBD59S2. The size of the chromosome is 5283,933 bp with a 35.4% GC content, consisting of 5938 genes and 5550 protein-coding sequences (CDSs), 25 complete rRNAs (5S, 16S, 23S), 98 tRNAs, 5 ncRNAs, 260 pseudo-genes, and 356 subsystems. Complete plasmid sequence of pBTBD59S1 comprises a total size of 162,149 bp with 33.4% GC content, 192 CDSs, and 13 subsystems. The other plasmid pBTBD59S2, is 199,209 bp long with 32.9% GC content, 179 CDSs, and 11 subsystems. Analyses by NCBI microbial genome BLAST, phylogenetic genome tree, and BLAST ring image generator (BRIG) revealed that BD59S belongs to
Bacillus cereus
group, and is more close to
B. thuringiensis
. Further, the strain possesses 57.04 kDa and 54.42 kDa Cry protein-coding genes, which show significant similarities with cancer cell-killing parasporin proteins of
B. thuringiensis
strains.</description><identifier>ISSN: 2190-572X</identifier><identifier>EISSN: 2190-5738</identifier><identifier>DOI: 10.1007/s13205-019-1849-7</identifier><identifier>PMID: 31406640</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Agriculture ; Bacillus ; Bacillus cereus ; Bacillus thuringiensis ; Bacteria ; Bioinformatics ; Biomaterials ; Biotechnology ; Cancer ; Cancer Research ; Chemistry ; Chemistry and Materials Science ; Chromosomes ; CRY protein ; Crystal structure ; Crystallinity ; Gene sequencing ; Genes ; Genome Reports ; Genomes ; Image processors ; Microorganisms ; neoplasm cells ; neoplasms ; Nucleotide sequence ; nucleotide sequences ; Phylogeny ; Plasmids ; Proteins ; ribosomal RNA ; Rings (mathematics) ; sequence analysis ; Stem Cells ; Subsystems ; transfer RNA</subject><ispartof>3 Biotech, 2019-09, Vol.9 (9), p.318-8, Article 318</ispartof><rights>King Abdulaziz City for Science and Technology 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-61e4c80c1d1670f7cbe621744befae087907b44ef538180bc1593d1d587e2fdc3</citedby><cites>FETCH-LOGICAL-c470t-61e4c80c1d1670f7cbe621744befae087907b44ef538180bc1593d1d587e2fdc3</cites><orcidid>0000-0001-7104-990X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682183/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682183/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27915,27916,41479,42548,51310,53782,53784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31406640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hasan, Md. Mahmud</creatorcontrib><creatorcontrib>Khan, Shakila Nargis</creatorcontrib><creatorcontrib>Karim, Muhammad Manjurul</creatorcontrib><creatorcontrib>Begum, Anowara</creatorcontrib><creatorcontrib>Hoq, Md. Mozammel</creatorcontrib><title>Complete genome and plasmid sequence of a novel Bacillus sp. BD59S, a parasporal protein synthesizing bacterium</title><title>3 Biotech</title><addtitle>3 Biotech</addtitle><addtitle>3 Biotech</addtitle><description>Parasporal crystalline inclusion proteins of some
Bacillus
spp. are of paramount importance due to their insecticidal, nematocidal, and cancer cell killing capabilities. Here, we present a brief report of the complete genome sequence of
Bacillus
sp. BD59S, a bacterium that produced HeLa cell-killing parasporal crystalline inclusion proteins. From genome sequencing and assembly, we found that the bacterium has one circular chromosome and two large plasmids, pBTBD59S1 and pBTBD59S2. The size of the chromosome is 5283,933 bp with a 35.4% GC content, consisting of 5938 genes and 5550 protein-coding sequences (CDSs), 25 complete rRNAs (5S, 16S, 23S), 98 tRNAs, 5 ncRNAs, 260 pseudo-genes, and 356 subsystems. Complete plasmid sequence of pBTBD59S1 comprises a total size of 162,149 bp with 33.4% GC content, 192 CDSs, and 13 subsystems. The other plasmid pBTBD59S2, is 199,209 bp long with 32.9% GC content, 179 CDSs, and 11 subsystems. Analyses by NCBI microbial genome BLAST, phylogenetic genome tree, and BLAST ring image generator (BRIG) revealed that BD59S belongs to
Bacillus cereus
group, and is more close to
B. thuringiensis
. Further, the strain possesses 57.04 kDa and 54.42 kDa Cry protein-coding genes, which show significant similarities with cancer cell-killing parasporin proteins of
B. thuringiensis
strains.</description><subject>Agriculture</subject><subject>Bacillus</subject><subject>Bacillus cereus</subject><subject>Bacillus thuringiensis</subject><subject>Bacteria</subject><subject>Bioinformatics</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>Cancer Research</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromosomes</subject><subject>CRY protein</subject><subject>Crystal structure</subject><subject>Crystallinity</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genome Reports</subject><subject>Genomes</subject><subject>Image processors</subject><subject>Microorganisms</subject><subject>neoplasm cells</subject><subject>neoplasms</subject><subject>Nucleotide sequence</subject><subject>nucleotide sequences</subject><subject>Phylogeny</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>ribosomal RNA</subject><subject>Rings (mathematics)</subject><subject>sequence analysis</subject><subject>Stem Cells</subject><subject>Subsystems</subject><subject>transfer RNA</subject><issn>2190-572X</issn><issn>2190-5738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kUtr3TAQhUVpaUKaH9BNEXTTRZxqJNuSN4Xm9pFCIIu20J2Q5fGNgiy5kh1Ifn0Vbnr7gGgjwfnmaGYOIS-BnQJj8m0GwVlTMegqUHVXySfkkEPHqkYK9XT_5j8OyHHO16ycBpoO2HNyIKBmbVuzQxI3cZo9Lki3GOKE1ISBzt7kyQ00488Vg0UaR2poiDfo6Zmxzvs10zyf0rMPTff1pGizSSbPMRlP5xQXdIHm27BcYXZ3Lmxpb-yCya3TC_JsND7j8cN9RL5_-vhtc15dXH7-snl_UdlasqVqAWurmIUBWslGaXtsOci67nE0yJTsmOzrGsdGKFCst2UyMcDQKIl8HKw4Iu92vvPaTzhYDEtpTs_JTSbd6mic_lcJ7kpv441uW8VBiWLw5sEgxbKFvOjJZYvem4BxzZqLss6ua6Qq6Ov_0Ou4plDG05y3CqTgsi0U7CibYs4Jx30zwPR9onqXqC6J6vtEtSw1r_6eYl_xO78C8B2QixS2mP58_bjrL0horGg</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Hasan, Md. Mahmud</creator><creator>Khan, Shakila Nargis</creator><creator>Karim, Muhammad Manjurul</creator><creator>Begum, Anowara</creator><creator>Hoq, Md. Mozammel</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7104-990X</orcidid></search><sort><creationdate>20190901</creationdate><title>Complete genome and plasmid sequence of a novel Bacillus sp. BD59S, a parasporal protein synthesizing bacterium</title><author>Hasan, Md. Mahmud ; Khan, Shakila Nargis ; Karim, Muhammad Manjurul ; Begum, Anowara ; Hoq, Md. Mozammel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-61e4c80c1d1670f7cbe621744befae087907b44ef538180bc1593d1d587e2fdc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Agriculture</topic><topic>Bacillus</topic><topic>Bacillus cereus</topic><topic>Bacillus thuringiensis</topic><topic>Bacteria</topic><topic>Bioinformatics</topic><topic>Biomaterials</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>Cancer Research</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromosomes</topic><topic>CRY protein</topic><topic>Crystal structure</topic><topic>Crystallinity</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genome Reports</topic><topic>Genomes</topic><topic>Image processors</topic><topic>Microorganisms</topic><topic>neoplasm cells</topic><topic>neoplasms</topic><topic>Nucleotide sequence</topic><topic>nucleotide sequences</topic><topic>Phylogeny</topic><topic>Plasmids</topic><topic>Proteins</topic><topic>ribosomal RNA</topic><topic>Rings (mathematics)</topic><topic>sequence analysis</topic><topic>Stem Cells</topic><topic>Subsystems</topic><topic>transfer RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hasan, Md. Mahmud</creatorcontrib><creatorcontrib>Khan, Shakila Nargis</creatorcontrib><creatorcontrib>Karim, Muhammad Manjurul</creatorcontrib><creatorcontrib>Begum, Anowara</creatorcontrib><creatorcontrib>Hoq, Md. Mozammel</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>3 Biotech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hasan, Md. Mahmud</au><au>Khan, Shakila Nargis</au><au>Karim, Muhammad Manjurul</au><au>Begum, Anowara</au><au>Hoq, Md. Mozammel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete genome and plasmid sequence of a novel Bacillus sp. BD59S, a parasporal protein synthesizing bacterium</atitle><jtitle>3 Biotech</jtitle><stitle>3 Biotech</stitle><addtitle>3 Biotech</addtitle><date>2019-09-01</date><risdate>2019</risdate><volume>9</volume><issue>9</issue><spage>318</spage><epage>8</epage><pages>318-8</pages><artnum>318</artnum><issn>2190-572X</issn><eissn>2190-5738</eissn><abstract>Parasporal crystalline inclusion proteins of some
Bacillus
spp. are of paramount importance due to their insecticidal, nematocidal, and cancer cell killing capabilities. Here, we present a brief report of the complete genome sequence of
Bacillus
sp. BD59S, a bacterium that produced HeLa cell-killing parasporal crystalline inclusion proteins. From genome sequencing and assembly, we found that the bacterium has one circular chromosome and two large plasmids, pBTBD59S1 and pBTBD59S2. The size of the chromosome is 5283,933 bp with a 35.4% GC content, consisting of 5938 genes and 5550 protein-coding sequences (CDSs), 25 complete rRNAs (5S, 16S, 23S), 98 tRNAs, 5 ncRNAs, 260 pseudo-genes, and 356 subsystems. Complete plasmid sequence of pBTBD59S1 comprises a total size of 162,149 bp with 33.4% GC content, 192 CDSs, and 13 subsystems. The other plasmid pBTBD59S2, is 199,209 bp long with 32.9% GC content, 179 CDSs, and 11 subsystems. Analyses by NCBI microbial genome BLAST, phylogenetic genome tree, and BLAST ring image generator (BRIG) revealed that BD59S belongs to
Bacillus cereus
group, and is more close to
B. thuringiensis
. Further, the strain possesses 57.04 kDa and 54.42 kDa Cry protein-coding genes, which show significant similarities with cancer cell-killing parasporin proteins of
B. thuringiensis
strains.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>31406640</pmid><doi>10.1007/s13205-019-1849-7</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-7104-990X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | EZB-FREE-00999 freely available EZB journals; PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Bacillus Bacillus cereus Bacillus thuringiensis Bacteria Bioinformatics Biomaterials Biotechnology Cancer Cancer Research Chemistry Chemistry and Materials Science Chromosomes CRY protein Crystal structure Crystallinity Gene sequencing Genes Genome Reports Genomes Image processors Microorganisms neoplasm cells neoplasms Nucleotide sequence nucleotide sequences Phylogeny Plasmids Proteins ribosomal RNA Rings (mathematics) sequence analysis Stem Cells Subsystems transfer RNA |
| title | Complete genome and plasmid sequence of a novel Bacillus sp. BD59S, a parasporal protein synthesizing bacterium |
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