Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties
A straightforward and green method for the synthesis of gold, silver, and silver chloride nanoparticles (Au NPs and Ag/AgCl NPs) was developed using three different microbial exopolymers (EP) as reducing and stabilizing agents. The exopolysaccharides and and the poly- -glutamic acid were produced by...
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| Veröffentlicht in: | Polymers 2019-07, Vol.11 (7), p.1157 |
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| creator | Scala, Angela Piperno, Anna Hada, Alexandru Astilean, Simion Vulpoi, Adriana Ginestra, Giovanna Marino, Andreana Nostro, Antonia Zammuto, Vincenzo Gugliandolo, Concetta |
| description | A straightforward and green method for the synthesis of gold, silver, and silver chloride nanoparticles (Au NPs and Ag/AgCl NPs) was developed using three different microbial exopolymers (EP) as reducing and stabilizing agents. The exopolysaccharides
and
and the poly-
-glutamic acid
were produced by thermophilic bacteria isolated from shallow hydrothermal vents off the Eolian Islands (Italy) in the Mediterranean Sea. The production of metal NPs was monitored by UV-Vis measurements by the typical plasmon resonance absorption peak and their antimicrobial activity towards Gram-positive and Gram- negative bacteria (
and
), as well as fungi (
) was investigated. The biological evaluation showed no activity for EP-Au NPs, except against
, whereas EP-Ag NPs exhibited a broad-spectrum of activity. The chemical composition, morphology, and size of EP-Ag NPs were investigated by UV-Vis, zeta potential (ζ), dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM). The best antimicrobial results were obtained for
and
(Minimum Inhibitory Concentration, MIC: 9.37-45 µg/mL; Minimum Bactericidal Concentration/Minimum Fungicidal Concentration, MBC/MFC: 11.25-75 µg/mL). |
| doi_str_mv | 10.3390/polym11071157 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6680601</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2557220708</sourcerecordid><originalsourceid>FETCH-LOGICAL-c415t-c90c74fe519f4c3f3d8c2e7aa04aee198917ab2ccb495182546655c0cb8da7ab3</originalsourceid><addsrcrecordid>eNpdkc1P3DAQxa2KqqAtx16RpV64hNrxR5JLJVhtAYndIrU9W44zYY0SO9he6P73mI-uoL6MpfebNx4_hL5QcsJYQ75NftiOlJKKUlF9QAclqVjBmSR7b-776DDGW5IPF1LS6hPaZ7SsuRT0AE1LHawDfKZNgmD1gBd__bMthFgsobM6QYfPA4DDv7YurSHaiH2PV74dAC8h5Z6Vdn7SIVkzQMQPNq3xqUt2tCb49sn0OvgJsg7xM_rY6yHC4WudoT8_Fr_nF8XVz_PL-elVYTgVqTANMRXvQdCm54b1rKtNCZXWhGsA2tQNrXRbGtPyRtC6FFxKIQwxbd3prLAZ-v7iO23aEToDLgU9qCnYUYet8tqq94qza3Xj75WUNZGEZoPjV4Pg7zYQkxptNDAM2oHfRFXmmYJIzpqMfv0PvfWb4PJ6qhSiKnMUpM5U8ULlT4kxQL97DCXqKU71Ls7MH73dYEf_C489At88nls</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2557220708</pqid></control><display><type>article</type><title>Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>PubMed Central</source><creator>Scala, Angela ; Piperno, Anna ; Hada, Alexandru ; Astilean, Simion ; Vulpoi, Adriana ; Ginestra, Giovanna ; Marino, Andreana ; Nostro, Antonia ; Zammuto, Vincenzo ; Gugliandolo, Concetta</creator><creatorcontrib>Scala, Angela ; Piperno, Anna ; Hada, Alexandru ; Astilean, Simion ; Vulpoi, Adriana ; Ginestra, Giovanna ; Marino, Andreana ; Nostro, Antonia ; Zammuto, Vincenzo ; Gugliandolo, Concetta</creatorcontrib><description>A straightforward and green method for the synthesis of gold, silver, and silver chloride nanoparticles (Au NPs and Ag/AgCl NPs) was developed using three different microbial exopolymers (EP) as reducing and stabilizing agents. The exopolysaccharides
and
and the poly-
-glutamic acid
were produced by thermophilic bacteria isolated from shallow hydrothermal vents off the Eolian Islands (Italy) in the Mediterranean Sea. The production of metal NPs was monitored by UV-Vis measurements by the typical plasmon resonance absorption peak and their antimicrobial activity towards Gram-positive and Gram- negative bacteria (
and
), as well as fungi (
) was investigated. The biological evaluation showed no activity for EP-Au NPs, except against
, whereas EP-Ag NPs exhibited a broad-spectrum of activity. The chemical composition, morphology, and size of EP-Ag NPs were investigated by UV-Vis, zeta potential (ζ), dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM). The best antimicrobial results were obtained for
and
(Minimum Inhibitory Concentration, MIC: 9.37-45 µg/mL; Minimum Bactericidal Concentration/Minimum Fungicidal Concentration, MBC/MFC: 11.25-75 µg/mL).</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym11071157</identifier><identifier>PMID: 31284651</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Amino acids ; Antiinfectives and antibacterials ; Antimicrobial agents ; Bacteria ; Bioassays ; Biological activity ; Carbohydrates ; Chemical activity ; Chemical composition ; Coliforms ; E coli ; Fungicides ; Glucose ; Glutamic acid ; Gold ; Lasers ; Microorganisms ; Morphology ; Nanoparticles ; Nanotechnology ; Noble metals ; Photon correlation spectroscopy ; Proteins ; Pseudomonas aeruginosa ; Resonance absorption ; Silver chloride ; Stabilizers (agents) ; Surfactants ; Synthesis ; Thermophilic bacteria ; Transmission electron microscopy ; Vents ; Zeta potential</subject><ispartof>Polymers, 2019-07, Vol.11 (7), p.1157</ispartof><rights>2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-c90c74fe519f4c3f3d8c2e7aa04aee198917ab2ccb495182546655c0cb8da7ab3</citedby><cites>FETCH-LOGICAL-c415t-c90c74fe519f4c3f3d8c2e7aa04aee198917ab2ccb495182546655c0cb8da7ab3</cites><orcidid>0000-0001-8751-8811 ; 0000-0002-9975-5651 ; 0000-0001-5334-4980 ; 0000-0002-9125-3999 ; 0000-0001-6004-5196 ; 0000-0003-2171-9033 ; 0000-0003-2382-5905</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680601/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680601/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31284651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Scala, Angela</creatorcontrib><creatorcontrib>Piperno, Anna</creatorcontrib><creatorcontrib>Hada, Alexandru</creatorcontrib><creatorcontrib>Astilean, Simion</creatorcontrib><creatorcontrib>Vulpoi, Adriana</creatorcontrib><creatorcontrib>Ginestra, Giovanna</creatorcontrib><creatorcontrib>Marino, Andreana</creatorcontrib><creatorcontrib>Nostro, Antonia</creatorcontrib><creatorcontrib>Zammuto, Vincenzo</creatorcontrib><creatorcontrib>Gugliandolo, Concetta</creatorcontrib><title>Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties</title><title>Polymers</title><addtitle>Polymers (Basel)</addtitle><description>A straightforward and green method for the synthesis of gold, silver, and silver chloride nanoparticles (Au NPs and Ag/AgCl NPs) was developed using three different microbial exopolymers (EP) as reducing and stabilizing agents. The exopolysaccharides
and
and the poly-
-glutamic acid
were produced by thermophilic bacteria isolated from shallow hydrothermal vents off the Eolian Islands (Italy) in the Mediterranean Sea. The production of metal NPs was monitored by UV-Vis measurements by the typical plasmon resonance absorption peak and their antimicrobial activity towards Gram-positive and Gram- negative bacteria (
and
), as well as fungi (
) was investigated. The biological evaluation showed no activity for EP-Au NPs, except against
, whereas EP-Ag NPs exhibited a broad-spectrum of activity. The chemical composition, morphology, and size of EP-Ag NPs were investigated by UV-Vis, zeta potential (ζ), dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM). The best antimicrobial results were obtained for
and
(Minimum Inhibitory Concentration, MIC: 9.37-45 µg/mL; Minimum Bactericidal Concentration/Minimum Fungicidal Concentration, MBC/MFC: 11.25-75 µg/mL).</description><subject>Amino acids</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Bioassays</subject><subject>Biological activity</subject><subject>Carbohydrates</subject><subject>Chemical activity</subject><subject>Chemical composition</subject><subject>Coliforms</subject><subject>E coli</subject><subject>Fungicides</subject><subject>Glucose</subject><subject>Glutamic acid</subject><subject>Gold</subject><subject>Lasers</subject><subject>Microorganisms</subject><subject>Morphology</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Noble metals</subject><subject>Photon correlation spectroscopy</subject><subject>Proteins</subject><subject>Pseudomonas aeruginosa</subject><subject>Resonance absorption</subject><subject>Silver chloride</subject><subject>Stabilizers (agents)</subject><subject>Surfactants</subject><subject>Synthesis</subject><subject>Thermophilic bacteria</subject><subject>Transmission electron microscopy</subject><subject>Vents</subject><subject>Zeta potential</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkc1P3DAQxa2KqqAtx16RpV64hNrxR5JLJVhtAYndIrU9W44zYY0SO9he6P73mI-uoL6MpfebNx4_hL5QcsJYQ75NftiOlJKKUlF9QAclqVjBmSR7b-776DDGW5IPF1LS6hPaZ7SsuRT0AE1LHawDfKZNgmD1gBd__bMthFgsobM6QYfPA4DDv7YurSHaiH2PV74dAC8h5Z6Vdn7SIVkzQMQPNq3xqUt2tCb49sn0OvgJsg7xM_rY6yHC4WudoT8_Fr_nF8XVz_PL-elVYTgVqTANMRXvQdCm54b1rKtNCZXWhGsA2tQNrXRbGtPyRtC6FFxKIQwxbd3prLAZ-v7iO23aEToDLgU9qCnYUYet8tqq94qza3Xj75WUNZGEZoPjV4Pg7zYQkxptNDAM2oHfRFXmmYJIzpqMfv0PvfWb4PJ6qhSiKnMUpM5U8ULlT4kxQL97DCXqKU71Ls7MH73dYEf_C489At88nls</recordid><startdate>20190707</startdate><enddate>20190707</enddate><creator>Scala, Angela</creator><creator>Piperno, Anna</creator><creator>Hada, Alexandru</creator><creator>Astilean, Simion</creator><creator>Vulpoi, Adriana</creator><creator>Ginestra, Giovanna</creator><creator>Marino, Andreana</creator><creator>Nostro, Antonia</creator><creator>Zammuto, Vincenzo</creator><creator>Gugliandolo, Concetta</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8751-8811</orcidid><orcidid>https://orcid.org/0000-0002-9975-5651</orcidid><orcidid>https://orcid.org/0000-0001-5334-4980</orcidid><orcidid>https://orcid.org/0000-0002-9125-3999</orcidid><orcidid>https://orcid.org/0000-0001-6004-5196</orcidid><orcidid>https://orcid.org/0000-0003-2171-9033</orcidid><orcidid>https://orcid.org/0000-0003-2382-5905</orcidid></search><sort><creationdate>20190707</creationdate><title>Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties</title><author>Scala, Angela ; Piperno, Anna ; Hada, Alexandru ; Astilean, Simion ; Vulpoi, Adriana ; Ginestra, Giovanna ; Marino, Andreana ; Nostro, Antonia ; Zammuto, Vincenzo ; Gugliandolo, Concetta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-c90c74fe519f4c3f3d8c2e7aa04aee198917ab2ccb495182546655c0cb8da7ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Amino acids</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Bioassays</topic><topic>Biological activity</topic><topic>Carbohydrates</topic><topic>Chemical activity</topic><topic>Chemical composition</topic><topic>Coliforms</topic><topic>E coli</topic><topic>Fungicides</topic><topic>Glucose</topic><topic>Glutamic acid</topic><topic>Gold</topic><topic>Lasers</topic><topic>Microorganisms</topic><topic>Morphology</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Noble metals</topic><topic>Photon correlation spectroscopy</topic><topic>Proteins</topic><topic>Pseudomonas aeruginosa</topic><topic>Resonance absorption</topic><topic>Silver chloride</topic><topic>Stabilizers (agents)</topic><topic>Surfactants</topic><topic>Synthesis</topic><topic>Thermophilic bacteria</topic><topic>Transmission electron microscopy</topic><topic>Vents</topic><topic>Zeta potential</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scala, Angela</creatorcontrib><creatorcontrib>Piperno, Anna</creatorcontrib><creatorcontrib>Hada, Alexandru</creatorcontrib><creatorcontrib>Astilean, Simion</creatorcontrib><creatorcontrib>Vulpoi, Adriana</creatorcontrib><creatorcontrib>Ginestra, Giovanna</creatorcontrib><creatorcontrib>Marino, Andreana</creatorcontrib><creatorcontrib>Nostro, Antonia</creatorcontrib><creatorcontrib>Zammuto, Vincenzo</creatorcontrib><creatorcontrib>Gugliandolo, Concetta</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scala, Angela</au><au>Piperno, Anna</au><au>Hada, Alexandru</au><au>Astilean, Simion</au><au>Vulpoi, Adriana</au><au>Ginestra, Giovanna</au><au>Marino, Andreana</au><au>Nostro, Antonia</au><au>Zammuto, Vincenzo</au><au>Gugliandolo, Concetta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties</atitle><jtitle>Polymers</jtitle><addtitle>Polymers (Basel)</addtitle><date>2019-07-07</date><risdate>2019</risdate><volume>11</volume><issue>7</issue><spage>1157</spage><pages>1157-</pages><issn>2073-4360</issn><eissn>2073-4360</eissn><abstract>A straightforward and green method for the synthesis of gold, silver, and silver chloride nanoparticles (Au NPs and Ag/AgCl NPs) was developed using three different microbial exopolymers (EP) as reducing and stabilizing agents. The exopolysaccharides
and
and the poly-
-glutamic acid
were produced by thermophilic bacteria isolated from shallow hydrothermal vents off the Eolian Islands (Italy) in the Mediterranean Sea. The production of metal NPs was monitored by UV-Vis measurements by the typical plasmon resonance absorption peak and their antimicrobial activity towards Gram-positive and Gram- negative bacteria (
and
), as well as fungi (
) was investigated. The biological evaluation showed no activity for EP-Au NPs, except against
, whereas EP-Ag NPs exhibited a broad-spectrum of activity. The chemical composition, morphology, and size of EP-Ag NPs were investigated by UV-Vis, zeta potential (ζ), dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM). The best antimicrobial results were obtained for
and
(Minimum Inhibitory Concentration, MIC: 9.37-45 µg/mL; Minimum Bactericidal Concentration/Minimum Fungicidal Concentration, MBC/MFC: 11.25-75 µg/mL).</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>31284651</pmid><doi>10.3390/polym11071157</doi><orcidid>https://orcid.org/0000-0001-8751-8811</orcidid><orcidid>https://orcid.org/0000-0002-9975-5651</orcidid><orcidid>https://orcid.org/0000-0001-5334-4980</orcidid><orcidid>https://orcid.org/0000-0002-9125-3999</orcidid><orcidid>https://orcid.org/0000-0001-6004-5196</orcidid><orcidid>https://orcid.org/0000-0003-2171-9033</orcidid><orcidid>https://orcid.org/0000-0003-2382-5905</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Amino acids Antiinfectives and antibacterials Antimicrobial agents Bacteria Bioassays Biological activity Carbohydrates Chemical activity Chemical composition Coliforms E coli Fungicides Glucose Glutamic acid Gold Lasers Microorganisms Morphology Nanoparticles Nanotechnology Noble metals Photon correlation spectroscopy Proteins Pseudomonas aeruginosa Resonance absorption Silver chloride Stabilizers (agents) Surfactants Synthesis Thermophilic bacteria Transmission electron microscopy Vents Zeta potential |
| title | Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties |
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