High-affinity free ubiquitin sensors for quantifying ubiquitin homeostasis and deubiquitination

Ubiquitin (Ub) conjugation is an essential post-translational modification that affects nearly all proteins in eukaryotes. The functions and mechanisms of ubiquitination are areas of extensive study, and yet the dynamics and regulation of even free (that is, unconjugated) Ub are poorly understood. A...

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Veröffentlicht in:	Nature methods 2019-08, Vol.16 (8), p.771-777
Hauptverfasser:	Choi, Yun-Seok, Bollinger, Sarah A., Prada, Luisa F., Scavone, Francesco, Yao, Tingting, Cohen, Robert E.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1888 631/45 631/45/612 Analysis Avidity Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biosensing Techniques Conjugates Conjugation Eukaryotes Fluorescence HeLa Cells Homeostasis Humans Life Sciences Lysates Post-translation Post-translational modification Protein Binding Protein Conformation Protein Processing, Post-Translational Proteins Proteins - chemistry Proteins - metabolism Proteomics Sensors Structure Ubiquitin Ubiquitin - metabolism Ubiquitination Workflow
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