Novel high-coverage targeted metabolomics method (SWATHtoMRM) for exploring follicular fluid metabolome alterations in women with recurrent spontaneous abortion undergoing in vitro fertilization
The complexity of follicular fluid metabolome presents a significant challenge for qualitative and quantitative metabolite profiling, and for discovering the comprehensive biomarkers. In order to address this challenge, a novel SWATHtoMRM metabolomics method was used for providing broad coverage and...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2019-07, Vol.9 (1), p.10873-12, Article 10873 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 12 |
|---|---|
| container_issue | 1 |
| container_start_page | 10873 |
| container_title | Scientific reports |
| container_volume | 9 |
| creator | Song, Jingyan Wang, Xiaoming Guo, Ying Yang, Yi Xu, Kaiyue Wang, Tianqi Sa, Yuanhong Yuan, Lihua Jiang, Huaying Guo, Jiayin Sun, Zhengao |
| description | The complexity of follicular fluid metabolome presents a significant challenge for qualitative and quantitative metabolite profiling, and for discovering the comprehensive biomarkers. In order to address this challenge, a novel SWATHtoMRM metabolomics method was used for providing broad coverage and excellent quantitative capability to discover the human follicular fluid metabolites related to recurrent spontaneous abortion (RSA) after
in vitro
fertilization and embryo transfer, and to evaluate their relationship with pregnancy outcome. The follicular fluid samples from the spontaneous abortion group (n = 22) and the control group (n = 22) were analyzed using ultra-performance liquid chromatography high-resolution mass spectrometry. A novel, high-coverage, targeted metabolomics method (SWATH to MRM) and a targeted metabolomics method were used to find and validate the differential metabolites between the two groups. A total of 18 follicular fluid metabolites, including amino acids, cholesterol, vitamins, fatty acids, cholic acid, lysophosphatidylcholine and other metabolites, were identified. In the RSA group, 8 metabolites, namely dehydroepiandrosterone, lysoPC(16:0), lysoPC(18:2), lysoPC(18:1), lysoPC(18:0), lysoPC(20:5), lysoPC(20:4), and lysoPC(20:3), were up-regulated, and 10 metabolites, namely phenylalanine, linoleate, oleic acid, docosahexaenoic acid, lithocholic acid, 25-hydroxyvitamin D3, hydroxycholesterol, 13-hydroxy-alpha-tocopherol, leucine, and tryptophan, were down-regulated. These differential metabolites related to RSA may provide a possible diagnostic basis and therapeutic target for RSA, as well as a scientific basis for elucidating the mechanism of RSA. |
| doi_str_mv | 10.1038/s41598-019-47370-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6659694</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2265795075</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-34779aa1d9e622be03424968c4ee0362af62b0b3ffe7930793e9f838e96582cb3</originalsourceid><addsrcrecordid>eNp9Ul9v1yAUbYzGLXNfwAdD4st8qFKgpbyYLIs6k00TnfGRUHrbstBSgf788_H8ZNJ1zp8-SALcyz3nwA0nyx4X-HmBaf0isKIUdY4LkTNOOc75veyQYFbmhBJyfy8-yI5DuMZplESwQjzMDmhBy1Tmh9nPd24HFg2mH3KdQq96QFH5HiK0aISoGmfdaHRYk8G16OTj59Or8-guP1w-Q53zCL7N1nkz9Smz1ujFKo86u5g9PiBlYxKPxk0BmQl9TWdpNXFAHvTiPUwRhdlNUU3gloASz69otEwt-N6t-om3M9E71EGqWfPjRu9R9qBTNsDx7X6UfXr96ursPL94_-bt2elFrhlnMaeMc6FU0QqoCGkAU0aYqGrNIMUVUV1FGtzQrgMuKE4TRFfTGkRV1kQ39Ch7uenOSzNCq9OLvbJy9mZU_rt0ysi_K5MZZO92sqpKUQmWBE5uBbz7skCIcjRBg7Vby5KQquSixLxM0Kf_QK_d4qfU3opipSAUi4QiG0p7F4KH7u4xBZarS-TmEplcIm9cInkiPdlv447y2xMJQDdAmNdfBf_n7v_I_gJS_M4m</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2264592309</pqid></control><display><type>article</type><title>Novel high-coverage targeted metabolomics method (SWATHtoMRM) for exploring follicular fluid metabolome alterations in women with recurrent spontaneous abortion undergoing in vitro fertilization</title><source>MEDLINE</source><source>Nature Free</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Directory of Open Access Journals</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature OA Free Journals</source><creator>Song, Jingyan ; Wang, Xiaoming ; Guo, Ying ; Yang, Yi ; Xu, Kaiyue ; Wang, Tianqi ; Sa, Yuanhong ; Yuan, Lihua ; Jiang, Huaying ; Guo, Jiayin ; Sun, Zhengao</creator><creatorcontrib>Song, Jingyan ; Wang, Xiaoming ; Guo, Ying ; Yang, Yi ; Xu, Kaiyue ; Wang, Tianqi ; Sa, Yuanhong ; Yuan, Lihua ; Jiang, Huaying ; Guo, Jiayin ; Sun, Zhengao</creatorcontrib><description>The complexity of follicular fluid metabolome presents a significant challenge for qualitative and quantitative metabolite profiling, and for discovering the comprehensive biomarkers. In order to address this challenge, a novel SWATHtoMRM metabolomics method was used for providing broad coverage and excellent quantitative capability to discover the human follicular fluid metabolites related to recurrent spontaneous abortion (RSA) after
in vitro
fertilization and embryo transfer, and to evaluate their relationship with pregnancy outcome. The follicular fluid samples from the spontaneous abortion group (n = 22) and the control group (n = 22) were analyzed using ultra-performance liquid chromatography high-resolution mass spectrometry. A novel, high-coverage, targeted metabolomics method (SWATH to MRM) and a targeted metabolomics method were used to find and validate the differential metabolites between the two groups. A total of 18 follicular fluid metabolites, including amino acids, cholesterol, vitamins, fatty acids, cholic acid, lysophosphatidylcholine and other metabolites, were identified. In the RSA group, 8 metabolites, namely dehydroepiandrosterone, lysoPC(16:0), lysoPC(18:2), lysoPC(18:1), lysoPC(18:0), lysoPC(20:5), lysoPC(20:4), and lysoPC(20:3), were up-regulated, and 10 metabolites, namely phenylalanine, linoleate, oleic acid, docosahexaenoic acid, lithocholic acid, 25-hydroxyvitamin D3, hydroxycholesterol, 13-hydroxy-alpha-tocopherol, leucine, and tryptophan, were down-regulated. These differential metabolites related to RSA may provide a possible diagnostic basis and therapeutic target for RSA, as well as a scientific basis for elucidating the mechanism of RSA.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-47370-7</identifier><identifier>PMID: 31350457</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647 ; 692/699 ; Abortion, Habitual - diagnosis ; Adult ; Amino acids ; Cholesterol ; Cholic acid ; Chromatography, High Pressure Liquid ; Dehydroepiandrosterone ; Dehydroepiandrosterone - metabolism ; Docosahexaenoic acid ; Down-Regulation ; Embryo transfer ; Fatty acids ; Female ; Fertilization in Vitro ; Follicular fluid ; Follicular Fluid - metabolism ; Humanities and Social Sciences ; Humans ; In vitro fertilization ; Leucine ; Liquid chromatography ; Lysophosphatidylcholine ; Mass Spectrometry ; Mass spectroscopy ; Metabolites ; Metabolome ; Metabolomics ; Metabolomics - methods ; Miscarriage ; multidisciplinary ; Oleic acid ; Phenylalanine ; Phenylalanine - metabolism ; Pregnancy ; Science ; Science (multidisciplinary) ; Therapeutic applications ; Tryptophan ; Up-Regulation ; Vitamins</subject><ispartof>Scientific reports, 2019-07, Vol.9 (1), p.10873-12, Article 10873</ispartof><rights>The Author(s) 2019</rights><rights>2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-34779aa1d9e622be03424968c4ee0362af62b0b3ffe7930793e9f838e96582cb3</citedby><cites>FETCH-LOGICAL-c474t-34779aa1d9e622be03424968c4ee0362af62b0b3ffe7930793e9f838e96582cb3</cites><orcidid>0000-0002-9723-1213</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659694/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659694/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31350457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Jingyan</creatorcontrib><creatorcontrib>Wang, Xiaoming</creatorcontrib><creatorcontrib>Guo, Ying</creatorcontrib><creatorcontrib>Yang, Yi</creatorcontrib><creatorcontrib>Xu, Kaiyue</creatorcontrib><creatorcontrib>Wang, Tianqi</creatorcontrib><creatorcontrib>Sa, Yuanhong</creatorcontrib><creatorcontrib>Yuan, Lihua</creatorcontrib><creatorcontrib>Jiang, Huaying</creatorcontrib><creatorcontrib>Guo, Jiayin</creatorcontrib><creatorcontrib>Sun, Zhengao</creatorcontrib><title>Novel high-coverage targeted metabolomics method (SWATHtoMRM) for exploring follicular fluid metabolome alterations in women with recurrent spontaneous abortion undergoing in vitro fertilization</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The complexity of follicular fluid metabolome presents a significant challenge for qualitative and quantitative metabolite profiling, and for discovering the comprehensive biomarkers. In order to address this challenge, a novel SWATHtoMRM metabolomics method was used for providing broad coverage and excellent quantitative capability to discover the human follicular fluid metabolites related to recurrent spontaneous abortion (RSA) after
in vitro
fertilization and embryo transfer, and to evaluate their relationship with pregnancy outcome. The follicular fluid samples from the spontaneous abortion group (n = 22) and the control group (n = 22) were analyzed using ultra-performance liquid chromatography high-resolution mass spectrometry. A novel, high-coverage, targeted metabolomics method (SWATH to MRM) and a targeted metabolomics method were used to find and validate the differential metabolites between the two groups. A total of 18 follicular fluid metabolites, including amino acids, cholesterol, vitamins, fatty acids, cholic acid, lysophosphatidylcholine and other metabolites, were identified. In the RSA group, 8 metabolites, namely dehydroepiandrosterone, lysoPC(16:0), lysoPC(18:2), lysoPC(18:1), lysoPC(18:0), lysoPC(20:5), lysoPC(20:4), and lysoPC(20:3), were up-regulated, and 10 metabolites, namely phenylalanine, linoleate, oleic acid, docosahexaenoic acid, lithocholic acid, 25-hydroxyvitamin D3, hydroxycholesterol, 13-hydroxy-alpha-tocopherol, leucine, and tryptophan, were down-regulated. These differential metabolites related to RSA may provide a possible diagnostic basis and therapeutic target for RSA, as well as a scientific basis for elucidating the mechanism of RSA.</description><subject>631/1647</subject><subject>692/699</subject><subject>Abortion, Habitual - diagnosis</subject><subject>Adult</subject><subject>Amino acids</subject><subject>Cholesterol</subject><subject>Cholic acid</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dehydroepiandrosterone</subject><subject>Dehydroepiandrosterone - metabolism</subject><subject>Docosahexaenoic acid</subject><subject>Down-Regulation</subject><subject>Embryo transfer</subject><subject>Fatty acids</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Follicular fluid</subject><subject>Follicular Fluid - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>In vitro fertilization</subject><subject>Leucine</subject><subject>Liquid chromatography</subject><subject>Lysophosphatidylcholine</subject><subject>Mass Spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Metabolome</subject><subject>Metabolomics</subject><subject>Metabolomics - methods</subject><subject>Miscarriage</subject><subject>multidisciplinary</subject><subject>Oleic acid</subject><subject>Phenylalanine</subject><subject>Phenylalanine - metabolism</subject><subject>Pregnancy</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Therapeutic applications</subject><subject>Tryptophan</subject><subject>Up-Regulation</subject><subject>Vitamins</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9Ul9v1yAUbYzGLXNfwAdD4st8qFKgpbyYLIs6k00TnfGRUHrbstBSgf788_H8ZNJ1zp8-SALcyz3nwA0nyx4X-HmBaf0isKIUdY4LkTNOOc75veyQYFbmhBJyfy8-yI5DuMZplESwQjzMDmhBy1Tmh9nPd24HFg2mH3KdQq96QFH5HiK0aISoGmfdaHRYk8G16OTj59Or8-guP1w-Q53zCL7N1nkz9Smz1ujFKo86u5g9PiBlYxKPxk0BmQl9TWdpNXFAHvTiPUwRhdlNUU3gloASz69otEwt-N6t-om3M9E71EGqWfPjRu9R9qBTNsDx7X6UfXr96ursPL94_-bt2elFrhlnMaeMc6FU0QqoCGkAU0aYqGrNIMUVUV1FGtzQrgMuKE4TRFfTGkRV1kQ39Ch7uenOSzNCq9OLvbJy9mZU_rt0ysi_K5MZZO92sqpKUQmWBE5uBbz7skCIcjRBg7Vby5KQquSixLxM0Kf_QK_d4qfU3opipSAUi4QiG0p7F4KH7u4xBZarS-TmEplcIm9cInkiPdlv447y2xMJQDdAmNdfBf_n7v_I_gJS_M4m</recordid><startdate>20190726</startdate><enddate>20190726</enddate><creator>Song, Jingyan</creator><creator>Wang, Xiaoming</creator><creator>Guo, Ying</creator><creator>Yang, Yi</creator><creator>Xu, Kaiyue</creator><creator>Wang, Tianqi</creator><creator>Sa, Yuanhong</creator><creator>Yuan, Lihua</creator><creator>Jiang, Huaying</creator><creator>Guo, Jiayin</creator><creator>Sun, Zhengao</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9723-1213</orcidid></search><sort><creationdate>20190726</creationdate><title>Novel high-coverage targeted metabolomics method (SWATHtoMRM) for exploring follicular fluid metabolome alterations in women with recurrent spontaneous abortion undergoing in vitro fertilization</title><author>Song, Jingyan ; Wang, Xiaoming ; Guo, Ying ; Yang, Yi ; Xu, Kaiyue ; Wang, Tianqi ; Sa, Yuanhong ; Yuan, Lihua ; Jiang, Huaying ; Guo, Jiayin ; Sun, Zhengao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-34779aa1d9e622be03424968c4ee0362af62b0b3ffe7930793e9f838e96582cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>631/1647</topic><topic>692/699</topic><topic>Abortion, Habitual - diagnosis</topic><topic>Adult</topic><topic>Amino acids</topic><topic>Cholesterol</topic><topic>Cholic acid</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dehydroepiandrosterone</topic><topic>Dehydroepiandrosterone - metabolism</topic><topic>Docosahexaenoic acid</topic><topic>Down-Regulation</topic><topic>Embryo transfer</topic><topic>Fatty acids</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Follicular fluid</topic><topic>Follicular Fluid - metabolism</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>In vitro fertilization</topic><topic>Leucine</topic><topic>Liquid chromatography</topic><topic>Lysophosphatidylcholine</topic><topic>Mass Spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolites</topic><topic>Metabolome</topic><topic>Metabolomics</topic><topic>Metabolomics - methods</topic><topic>Miscarriage</topic><topic>multidisciplinary</topic><topic>Oleic acid</topic><topic>Phenylalanine</topic><topic>Phenylalanine - metabolism</topic><topic>Pregnancy</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Therapeutic applications</topic><topic>Tryptophan</topic><topic>Up-Regulation</topic><topic>Vitamins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Jingyan</creatorcontrib><creatorcontrib>Wang, Xiaoming</creatorcontrib><creatorcontrib>Guo, Ying</creatorcontrib><creatorcontrib>Yang, Yi</creatorcontrib><creatorcontrib>Xu, Kaiyue</creatorcontrib><creatorcontrib>Wang, Tianqi</creatorcontrib><creatorcontrib>Sa, Yuanhong</creatorcontrib><creatorcontrib>Yuan, Lihua</creatorcontrib><creatorcontrib>Jiang, Huaying</creatorcontrib><creatorcontrib>Guo, Jiayin</creatorcontrib><creatorcontrib>Sun, Zhengao</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Jingyan</au><au>Wang, Xiaoming</au><au>Guo, Ying</au><au>Yang, Yi</au><au>Xu, Kaiyue</au><au>Wang, Tianqi</au><au>Sa, Yuanhong</au><au>Yuan, Lihua</au><au>Jiang, Huaying</au><au>Guo, Jiayin</au><au>Sun, Zhengao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel high-coverage targeted metabolomics method (SWATHtoMRM) for exploring follicular fluid metabolome alterations in women with recurrent spontaneous abortion undergoing in vitro fertilization</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-07-26</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>10873</spage><epage>12</epage><pages>10873-12</pages><artnum>10873</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The complexity of follicular fluid metabolome presents a significant challenge for qualitative and quantitative metabolite profiling, and for discovering the comprehensive biomarkers. In order to address this challenge, a novel SWATHtoMRM metabolomics method was used for providing broad coverage and excellent quantitative capability to discover the human follicular fluid metabolites related to recurrent spontaneous abortion (RSA) after
in vitro
fertilization and embryo transfer, and to evaluate their relationship with pregnancy outcome. The follicular fluid samples from the spontaneous abortion group (n = 22) and the control group (n = 22) were analyzed using ultra-performance liquid chromatography high-resolution mass spectrometry. A novel, high-coverage, targeted metabolomics method (SWATH to MRM) and a targeted metabolomics method were used to find and validate the differential metabolites between the two groups. A total of 18 follicular fluid metabolites, including amino acids, cholesterol, vitamins, fatty acids, cholic acid, lysophosphatidylcholine and other metabolites, were identified. In the RSA group, 8 metabolites, namely dehydroepiandrosterone, lysoPC(16:0), lysoPC(18:2), lysoPC(18:1), lysoPC(18:0), lysoPC(20:5), lysoPC(20:4), and lysoPC(20:3), were up-regulated, and 10 metabolites, namely phenylalanine, linoleate, oleic acid, docosahexaenoic acid, lithocholic acid, 25-hydroxyvitamin D3, hydroxycholesterol, 13-hydroxy-alpha-tocopherol, leucine, and tryptophan, were down-regulated. These differential metabolites related to RSA may provide a possible diagnostic basis and therapeutic target for RSA, as well as a scientific basis for elucidating the mechanism of RSA.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31350457</pmid><doi>10.1038/s41598-019-47370-7</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-9723-1213</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2019-07, Vol.9 (1), p.10873-12, Article 10873 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6659694 |
| source | MEDLINE; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Directory of Open Access Journals; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 631/1647 692/699 Abortion, Habitual - diagnosis Adult Amino acids Cholesterol Cholic acid Chromatography, High Pressure Liquid Dehydroepiandrosterone Dehydroepiandrosterone - metabolism Docosahexaenoic acid Down-Regulation Embryo transfer Fatty acids Female Fertilization in Vitro Follicular fluid Follicular Fluid - metabolism Humanities and Social Sciences Humans In vitro fertilization Leucine Liquid chromatography Lysophosphatidylcholine Mass Spectrometry Mass spectroscopy Metabolites Metabolome Metabolomics Metabolomics - methods Miscarriage multidisciplinary Oleic acid Phenylalanine Phenylalanine - metabolism Pregnancy Science Science (multidisciplinary) Therapeutic applications Tryptophan Up-Regulation Vitamins |
| title | Novel high-coverage targeted metabolomics method (SWATHtoMRM) for exploring follicular fluid metabolome alterations in women with recurrent spontaneous abortion undergoing in vitro fertilization |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T21%3A25%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Novel%20high-coverage%20targeted%20metabolomics%20method%20(SWATHtoMRM)%20for%20exploring%20follicular%20fluid%20metabolome%20alterations%20in%20women%20with%20recurrent%20spontaneous%20abortion%20undergoing%20in%20vitro%20fertilization&rft.jtitle=Scientific%20reports&rft.au=Song,%20Jingyan&rft.date=2019-07-26&rft.volume=9&rft.issue=1&rft.spage=10873&rft.epage=12&rft.pages=10873-12&rft.artnum=10873&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-019-47370-7&rft_dat=%3Cproquest_pubme%3E2265795075%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2264592309&rft_id=info:pmid/31350457&rfr_iscdi=true |