Genotyping of samples lacking expected antibodies in ABO blood group
We report nine donations with ABO inconsistency in reverse typing caused by partly or entirely missing antibodies. A and B antigens and antibodies were examined by serological blood typing, and ABO deoxyribonucleic acid (DNA) analyses were performed by sequence‐specific priming and sequencing. A B10...
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| Veröffentlicht in: | Journal of clinical laboratory analysis 2007, Vol.21 (6), p.363-366 |
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| container_title | Journal of clinical laboratory analysis |
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| creator | Deng, Zhi-Hui Zeng, Jian-Qiang Yu, Qiong Su, Yu-Qing Liang, Yan-Lian Lu, Liang Zhu, Wei-Gang Yang, Bao-Cheng |
| description | We report nine donations with ABO inconsistency in reverse typing caused by partly or entirely missing antibodies. A and B antigens and antibodies were examined by serological blood typing, and ABO deoxyribonucleic acid (DNA) analyses were performed by sequence‐specific priming and sequencing. A B101 allele was demonstrable in a case with O phenotype. The molecular mechanisms in deficiency of natural ABO antibody could be partly clarified. The ABO genotyping technique is an accurate method for determining the blood samples involved in ABO grouping discrepancies and is a valuable complement to serology for correct determination of donor blood status. The mechanisms involved in the absence of potent natural antibodies directed against A and B antigen lacking on an individual's own red cell membranes remain to be further investigated. J. Clin. Lab. Anal. 21:363–366, 2007. © 2007 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jcla.20196 |
| format | Article |
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A and B antigens and antibodies were examined by serological blood typing, and ABO deoxyribonucleic acid (DNA) analyses were performed by sequence‐specific priming and sequencing. A B101 allele was demonstrable in a case with O phenotype. The molecular mechanisms in deficiency of natural ABO antibody could be partly clarified. The ABO genotyping technique is an accurate method for determining the blood samples involved in ABO grouping discrepancies and is a valuable complement to serology for correct determination of donor blood status. The mechanisms involved in the absence of potent natural antibodies directed against A and B antigen lacking on an individual's own red cell membranes remain to be further investigated. J. Clin. Lab. Anal. 21:363–366, 2007. © 2007 Wiley‐Liss, Inc.</description><identifier>ISSN: 0887-8013</identifier><identifier>EISSN: 1098-2825</identifier><identifier>DOI: 10.1002/jcla.20196</identifier><identifier>PMID: 18022920</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>ABO ; ABO allele ; ABO Blood-Group System - genetics ; Adolescent ; Adult ; Antibodies ; antibody ; Female ; Genotype ; Humans ; Male ; Original ; Phenotype</subject><ispartof>Journal of clinical laboratory analysis, 2007, Vol.21 (6), p.363-366</ispartof><rights>2007 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4466-9c8297bd208a591403b256c2b0f9bea4a4fb0b5a429ee6890fa1ceaba9dfb4f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6648999/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6648999/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,4024,27923,27924,27925,45574,45575,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18022920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deng, Zhi-Hui</creatorcontrib><creatorcontrib>Zeng, Jian-Qiang</creatorcontrib><creatorcontrib>Yu, Qiong</creatorcontrib><creatorcontrib>Su, Yu-Qing</creatorcontrib><creatorcontrib>Liang, Yan-Lian</creatorcontrib><creatorcontrib>Lu, Liang</creatorcontrib><creatorcontrib>Zhu, Wei-Gang</creatorcontrib><creatorcontrib>Yang, Bao-Cheng</creatorcontrib><title>Genotyping of samples lacking expected antibodies in ABO blood group</title><title>Journal of clinical laboratory analysis</title><addtitle>J. Clin. Lab. Anal</addtitle><description>We report nine donations with ABO inconsistency in reverse typing caused by partly or entirely missing antibodies. A and B antigens and antibodies were examined by serological blood typing, and ABO deoxyribonucleic acid (DNA) analyses were performed by sequence‐specific priming and sequencing. A B101 allele was demonstrable in a case with O phenotype. The molecular mechanisms in deficiency of natural ABO antibody could be partly clarified. The ABO genotyping technique is an accurate method for determining the blood samples involved in ABO grouping discrepancies and is a valuable complement to serology for correct determination of donor blood status. The mechanisms involved in the absence of potent natural antibodies directed against A and B antigen lacking on an individual's own red cell membranes remain to be further investigated. J. Clin. Lab. Anal. 21:363–366, 2007. © 2007 Wiley‐Liss, Inc.</description><subject>ABO</subject><subject>ABO allele</subject><subject>ABO Blood-Group System - genetics</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Antibodies</subject><subject>antibody</subject><subject>Female</subject><subject>Genotype</subject><subject>Humans</subject><subject>Male</subject><subject>Original</subject><subject>Phenotype</subject><issn>0887-8013</issn><issn>1098-2825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi1ERZeWCw-AcuKAlHbsOI59QVq2sKVaUZCKOFq2M1nceuMQZ6H79s2ySwsXehpp5vs_jfQT8pLCCQVgp9cumBMGVIknZEJByZxJVj4lE5CyyiXQ4pA8T-kaAKSi4hk5pBIYUwwm5GyObRw2nW-XWWyyZFZdwJQF4262K7zt0A1YZ6YdvI21H2--zabvLjMbYqyzZR_X3TE5aExI-GI_j8jXD--vZuf54nL-cTZd5I5zIXLlJFOVrRlIUyrKobCsFI5ZaJRFww1vLNjScKYQhVTQGOrQWKPqxvIGiiPyduft1naFtcN26E3QXe9Xpt_oaLz-99L673oZf2ohuFRKjYLXe0Eff6wxDXrlk8MQTItxnbSQJS-ULB8FGTAlSlaN4Jsd6PqYUo_N_TcU9LYdvW1H_25nhF_9_f8Duq9jBOgO-OUDbv6j0hezxfSPNN9lfBrw9j5j-hstqqIq9bdPc32mPl9J-UXoqrgDpwOqig</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Deng, Zhi-Hui</creator><creator>Zeng, Jian-Qiang</creator><creator>Yu, Qiong</creator><creator>Su, Yu-Qing</creator><creator>Liang, Yan-Lian</creator><creator>Lu, Liang</creator><creator>Zhu, Wei-Gang</creator><creator>Yang, Bao-Cheng</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2007</creationdate><title>Genotyping of samples lacking expected antibodies in ABO blood group</title><author>Deng, Zhi-Hui ; Zeng, Jian-Qiang ; Yu, Qiong ; Su, Yu-Qing ; Liang, Yan-Lian ; Lu, Liang ; Zhu, Wei-Gang ; Yang, Bao-Cheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4466-9c8297bd208a591403b256c2b0f9bea4a4fb0b5a429ee6890fa1ceaba9dfb4f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>ABO</topic><topic>ABO allele</topic><topic>ABO Blood-Group System - genetics</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Antibodies</topic><topic>antibody</topic><topic>Female</topic><topic>Genotype</topic><topic>Humans</topic><topic>Male</topic><topic>Original</topic><topic>Phenotype</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deng, Zhi-Hui</creatorcontrib><creatorcontrib>Zeng, Jian-Qiang</creatorcontrib><creatorcontrib>Yu, Qiong</creatorcontrib><creatorcontrib>Su, Yu-Qing</creatorcontrib><creatorcontrib>Liang, Yan-Lian</creatorcontrib><creatorcontrib>Lu, Liang</creatorcontrib><creatorcontrib>Zhu, Wei-Gang</creatorcontrib><creatorcontrib>Yang, Bao-Cheng</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical laboratory analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Zhi-Hui</au><au>Zeng, Jian-Qiang</au><au>Yu, Qiong</au><au>Su, Yu-Qing</au><au>Liang, Yan-Lian</au><au>Lu, Liang</au><au>Zhu, Wei-Gang</au><au>Yang, Bao-Cheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotyping of samples lacking expected antibodies in ABO blood group</atitle><jtitle>Journal of clinical laboratory analysis</jtitle><addtitle>J. Clin. Lab. Anal</addtitle><date>2007</date><risdate>2007</risdate><volume>21</volume><issue>6</issue><spage>363</spage><epage>366</epage><pages>363-366</pages><issn>0887-8013</issn><eissn>1098-2825</eissn><abstract>We report nine donations with ABO inconsistency in reverse typing caused by partly or entirely missing antibodies. A and B antigens and antibodies were examined by serological blood typing, and ABO deoxyribonucleic acid (DNA) analyses were performed by sequence‐specific priming and sequencing. A B101 allele was demonstrable in a case with O phenotype. The molecular mechanisms in deficiency of natural ABO antibody could be partly clarified. The ABO genotyping technique is an accurate method for determining the blood samples involved in ABO grouping discrepancies and is a valuable complement to serology for correct determination of donor blood status. The mechanisms involved in the absence of potent natural antibodies directed against A and B antigen lacking on an individual's own red cell membranes remain to be further investigated. J. Clin. Lab. Anal. 21:363–366, 2007. © 2007 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18022920</pmid><doi>10.1002/jcla.20196</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals; PubMed Central; EZB Electronic Journals Library |
| subjects | ABO ABO allele ABO Blood-Group System - genetics Adolescent Adult Antibodies antibody Female Genotype Humans Male Original Phenotype |
| title | Genotyping of samples lacking expected antibodies in ABO blood group |
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