Establishment of hepatocellular carcinoma patient-derived xenografts from image-guided percutaneous biopsies
While patient-derived xenograft (PDX) models of hepatocellular carcinoma (HCC) have been successfully generated from resected tissues, no reliable methods have been reported for the generation of PDXs from patients who are not candidates for resection and represent the vast majority of patients with...
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| Veröffentlicht in: | Scientific reports 2019-07, Vol.9 (1), p.10546-8, Article 10546 |
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| creator | Tischfield, David J. Ackerman, Daniel Noji, Michael Chen, James X. Johnson, Omar Perkons, Nicholas R. Nadolski, Gregory J. Hunt, Stephen J. Soulen, Michael C. Furth, Emma E. Gade, Terence P. |
| description | While patient-derived xenograft (PDX) models of hepatocellular carcinoma (HCC) have been successfully generated from resected tissues, no reliable methods have been reported for the generation of PDXs from patients who are not candidates for resection and represent the vast majority of patients with HCC. Here we compare two methods for the creation of PDXs from HCC biopsies and find that implantation of whole biopsy samples without the addition of basement membrane matrix favors the formation of PDX tumors that resemble Epstein-Barr virus (EBV)-driven B-cell lymphomas rather than HCC tumors. In contrast, implantation with Matrigel supports growth of HCC cells and leads to a high rate of HCC tumor formation from these biopsies. We validate the resulting PDXs, confirm their fidelity to the patients’ disease and conclude that minimally invasive percutaneous liver biopsies can be used with relatively high efficiency to generate PDXs of HCC. |
| doi_str_mv | 10.1038/s41598-019-47104-9 |
| format | Article |
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Here we compare two methods for the creation of PDXs from HCC biopsies and find that implantation of whole biopsy samples without the addition of basement membrane matrix favors the formation of PDX tumors that resemble Epstein-Barr virus (EBV)-driven B-cell lymphomas rather than HCC tumors. In contrast, implantation with Matrigel supports growth of HCC cells and leads to a high rate of HCC tumor formation from these biopsies. We validate the resulting PDXs, confirm their fidelity to the patients’ disease and conclude that minimally invasive percutaneous liver biopsies can be used with relatively high efficiency to generate PDXs of HCC.</description><subject>13/1</subject><subject>14/34</subject><subject>59/57</subject><subject>631/67/1504/1610/4029</subject><subject>631/67/70</subject><subject>64/60</subject><subject>82</subject><subject>82/51</subject><subject>82/80</subject><subject>Animals</subject><subject>B-cell lymphoma</subject><subject>Biopsy</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Carcinoma, Hepatocellular - virology</subject><subject>Collagen</subject><subject>Drug Combinations</subject><subject>Epstein-Barr virus</subject><subject>Hepatocellular carcinoma</subject><subject>Herpesvirus 4, Human - genetics</subject><subject>Herpesvirus 4, Human - isolation & purification</subject><subject>Heterografts</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Image-Guided Biopsy</subject><subject>Laminin</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tischfield, David J.</au><au>Ackerman, Daniel</au><au>Noji, Michael</au><au>Chen, James X.</au><au>Johnson, Omar</au><au>Perkons, Nicholas R.</au><au>Nadolski, Gregory J.</au><au>Hunt, Stephen J.</au><au>Soulen, Michael C.</au><au>Furth, Emma E.</au><au>Gade, Terence P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of hepatocellular carcinoma patient-derived xenografts from image-guided percutaneous biopsies</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-07-22</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>10546</spage><epage>8</epage><pages>10546-8</pages><artnum>10546</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>While patient-derived xenograft (PDX) models of hepatocellular carcinoma (HCC) have been successfully generated from resected tissues, no reliable methods have been reported for the generation of PDXs from patients who are not candidates for resection and represent the vast majority of patients with HCC. Here we compare two methods for the creation of PDXs from HCC biopsies and find that implantation of whole biopsy samples without the addition of basement membrane matrix favors the formation of PDX tumors that resemble Epstein-Barr virus (EBV)-driven B-cell lymphomas rather than HCC tumors. In contrast, implantation with Matrigel supports growth of HCC cells and leads to a high rate of HCC tumor formation from these biopsies. We validate the resulting PDXs, confirm their fidelity to the patients’ disease and conclude that minimally invasive percutaneous liver biopsies can be used with relatively high efficiency to generate PDXs of HCC.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31332214</pmid><doi>10.1038/s41598-019-47104-9</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/1 14/34 59/57 631/67/1504/1610/4029 631/67/70 64/60 82 82/51 82/80 Animals B-cell lymphoma Biopsy Carcinoma, Hepatocellular - pathology Carcinoma, Hepatocellular - virology Collagen Drug Combinations Epstein-Barr virus Hepatocellular carcinoma Herpesvirus 4, Human - genetics Herpesvirus 4, Human - isolation & purification Heterografts Humanities and Social Sciences Humans Image-Guided Biopsy Laminin Liver cancer Liver Neoplasms - pathology Liver Neoplasms - virology Liver Neoplasms, Experimental - pathology Liver Neoplasms, Experimental - virology Lymphocytes B Male Mice Mice, Inbred NOD Mice, SCID multidisciplinary Neoplasm Transplantation - methods Patients Proteoglycans Science Science (multidisciplinary) Tumors Xenograft Model Antitumor Assays Xenografts |
| title | Establishment of hepatocellular carcinoma patient-derived xenografts from image-guided percutaneous biopsies |
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