RNA silencing-mediated resistance to a crinivirus (Closteroviridae) in cultivated sweetpotato (Ipomoea batatas L.) and development of sweetpotato virus disease following co-infection with a potyvirus

Sweetpotato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) is one of the most important pathogens of sweetpotato (Ipomoea batatas L.). It can reduce yields by 50% by itself and cause various synergistic disease complexes when co-infecting with other viruses, including sweetp...

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Veröffentlicht in:Molecular plant pathology 2008-09, Vol.9 (5), p.589-598
Hauptverfasser: KREUZE, JAN F, KLEIN, ILANIT SAMOLSKI, LAZARO, MILTON UNTIVEROS, CHUQUIYURI, WILMER J. CUELLAR, MORGAN, GABRIELA LAJO, MEJÍA, PATRICIA G. CIPRIANI, GHISLAIN, MARC, VALKONEN, JARI P.T
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container_end_page 598
container_issue 5
container_start_page 589
container_title Molecular plant pathology
container_volume 9
creator KREUZE, JAN F
KLEIN, ILANIT SAMOLSKI
LAZARO, MILTON UNTIVEROS
CHUQUIYURI, WILMER J. CUELLAR
MORGAN, GABRIELA LAJO
MEJÍA, PATRICIA G. CIPRIANI
GHISLAIN, MARC
VALKONEN, JARI P.T
description Sweetpotato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) is one of the most important pathogens of sweetpotato (Ipomoea batatas L.). It can reduce yields by 50% by itself and cause various synergistic disease complexes when co-infecting with other viruses, including sweetpotato feathery mottle virus (SPFMV; genus Potyvirus, family Potyviridae). Because no sources of true resistance to SPCSV are available in sweetpotato germplasm, a pathogen-derived transgenic resistance strategy was tested as an alternative solution in this study. A Peruvian sweetpotato landrace 'Huachano' was transformed with an intron-spliced hairpin construct targeting the replicase encoding sequences of SPCSV and SPFMV using an improved genetic transformation procedure with reproducible efficiency. Twenty-eight independent transgenic events were obtained in three transformation experiments using a highly virulent Agrobacterium tumefaciens strain and regeneration through embryogenesis. Molecular analysis indicated that all regenerants were transgenic, with 1-7 transgene loci. Accumulation of transgene-specific siRNA was detected in most of them. None of the transgenic events was immune to SPCSV, but ten of the 20 tested transgenic events exhibited mild or no symptoms following infection, and accumulation of SPCSV was significantly reduced. There are few previous reports of RNA silencing-mediated transgenic resistance to viruses of Closteroviridae in cultivated plants. However, the high levels of resistance to accumulation of SPCSV could not prevent development of synergistic sweet potato virus disease in those transgenic plants also infected with SPFMV.
doi_str_mv 10.1111/j.1364-3703.2008.00480.x
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CUELLAR</au><au>MORGAN, GABRIELA LAJO</au><au>MEJÍA, PATRICIA G. CIPRIANI</au><au>GHISLAIN, MARC</au><au>VALKONEN, JARI P.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNA silencing-mediated resistance to a crinivirus (Closteroviridae) in cultivated sweetpotato (Ipomoea batatas L.) and development of sweetpotato virus disease following co-infection with a potyvirus</atitle><jtitle>Molecular plant pathology</jtitle><date>2008-09</date><risdate>2008</risdate><volume>9</volume><issue>5</issue><spage>589</spage><epage>598</epage><pages>589-598</pages><issn>1464-6722</issn><eissn>1364-3703</eissn><abstract>Sweetpotato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) is one of the most important pathogens of sweetpotato (Ipomoea batatas L.). It can reduce yields by 50% by itself and cause various synergistic disease complexes when co-infecting with other viruses, including sweetpotato feathery mottle virus (SPFMV; genus Potyvirus, family Potyviridae). Because no sources of true resistance to SPCSV are available in sweetpotato germplasm, a pathogen-derived transgenic resistance strategy was tested as an alternative solution in this study. A Peruvian sweetpotato landrace 'Huachano' was transformed with an intron-spliced hairpin construct targeting the replicase encoding sequences of SPCSV and SPFMV using an improved genetic transformation procedure with reproducible efficiency. Twenty-eight independent transgenic events were obtained in three transformation experiments using a highly virulent Agrobacterium tumefaciens strain and regeneration through embryogenesis. Molecular analysis indicated that all regenerants were transgenic, with 1-7 transgene loci. Accumulation of transgene-specific siRNA was detected in most of them. None of the transgenic events was immune to SPCSV, but ten of the 20 tested transgenic events exhibited mild or no symptoms following infection, and accumulation of SPCSV was significantly reduced. There are few previous reports of RNA silencing-mediated transgenic resistance to viruses of Closteroviridae in cultivated plants. However, the high levels of resistance to accumulation of SPCSV could not prevent development of synergistic sweet potato virus disease in those transgenic plants also infected with SPFMV.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>19018990</pmid><doi>10.1111/j.1364-3703.2008.00480.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
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ispartof Molecular plant pathology, 2008-09, Vol.9 (5), p.589-598
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source Wiley Online Library (Open Access Collection)
subjects Agrobacterium tumefaciens
Agronomy. Soil science and plant productions
Biological and medical sciences
Crinivirus
Fundamental and applied biological sciences. Psychology
Genetics and breeding of economic plants
Ipomoea batatas
Original
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Potyviridae
Potyvirus
Solanum tuberosum
Sweetpotato chlorotic stunt virus
title RNA silencing-mediated resistance to a crinivirus (Closteroviridae) in cultivated sweetpotato (Ipomoea batatas L.) and development of sweetpotato virus disease following co-infection with a potyvirus
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