A novel Meloidogyne graminicola effector, MgMO237, interacts with multiple host defence‐related proteins to manipulate plant basal immunity and promote parasitism
Summary Plant‐parasitic nematodes can secrete effector proteins into the host tissue to facilitate their parasitism. In this study, we report a novel effector protein, MgMO237, from Meloidogyne graminicola, which is exclusively expressed within the dorsal oesophageal gland cell and markedly up‐regul...
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| Veröffentlicht in: | Molecular plant pathology 2018-08, Vol.19 (8), p.1942-1955 |
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| container_end_page | 1955 |
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| container_issue | 8 |
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| container_title | Molecular plant pathology |
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| creator | Chen, Jiansong Hu, Lili Sun, Longhua Lin, Borong Huang, Kun Zhuo, Kan Liao, Jinling |
| description | Summary
Plant‐parasitic nematodes can secrete effector proteins into the host tissue to facilitate their parasitism. In this study, we report a novel effector protein, MgMO237, from Meloidogyne graminicola, which is exclusively expressed within the dorsal oesophageal gland cell and markedly up‐regulated in parasitic third‐/fourth‐stage juveniles of M. graminicola. Transient expression of MgMO237 in protoplasts from rice roots showed that MgMO237 was localized in the cytoplasm and nucleus of the host cells. Rice plants overexpressing MgMO237 showed an increased susceptibility to M. graminicola. In contrast, rice plants expressing RNA interference vectors targeting MgMO237 showed an increased resistance to M. graminicola. In addition, yeast two‐hybrid and co‐immunoprecipitation assays showed that MgMO237 interacted specifically with three rice endogenous proteins, i.e. 1,3‐β‐glucan synthase component (OsGSC), cysteine‐rich repeat secretory protein 55 (OsCRRSP55) and pathogenesis‐related BetvI family protein (OsBetvI), which are all related to host defences. Moreover, MgMO237 can suppress host defence responses, including the expression of host defence‐related genes, cell wall callose deposition and the burst of reactive oxygen species. These results demonstrate that the effector MgMO237 probably promotes the parasitism of M. graminicola by interacting with multiple host defence‐related proteins and suppressing plant basal immunity in the later parasitic stages of nematodes. |
| doi_str_mv | 10.1111/mpp.12671 |
| format | Article |
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Plant‐parasitic nematodes can secrete effector proteins into the host tissue to facilitate their parasitism. In this study, we report a novel effector protein, MgMO237, from Meloidogyne graminicola, which is exclusively expressed within the dorsal oesophageal gland cell and markedly up‐regulated in parasitic third‐/fourth‐stage juveniles of M. graminicola. Transient expression of MgMO237 in protoplasts from rice roots showed that MgMO237 was localized in the cytoplasm and nucleus of the host cells. Rice plants overexpressing MgMO237 showed an increased susceptibility to M. graminicola. In contrast, rice plants expressing RNA interference vectors targeting MgMO237 showed an increased resistance to M. graminicola. In addition, yeast two‐hybrid and co‐immunoprecipitation assays showed that MgMO237 interacted specifically with three rice endogenous proteins, i.e. 1,3‐β‐glucan synthase component (OsGSC), cysteine‐rich repeat secretory protein 55 (OsCRRSP55) and pathogenesis‐related BetvI family protein (OsBetvI), which are all related to host defences. Moreover, MgMO237 can suppress host defence responses, including the expression of host defence‐related genes, cell wall callose deposition and the burst of reactive oxygen species. These results demonstrate that the effector MgMO237 probably promotes the parasitism of M. graminicola by interacting with multiple host defence‐related proteins and suppressing plant basal immunity in the later parasitic stages of nematodes.</description><identifier>ISSN: 1464-6722</identifier><identifier>EISSN: 1364-3703</identifier><identifier>DOI: 10.1111/mpp.12671</identifier><identifier>PMID: 29485753</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Cytoplasm ; effector ; Esophagus ; Gene expression ; Glucan ; host defence suppression ; Host plants ; Immunity ; Immunoprecipitation ; interaction ; Meloidogyne graminicola ; Nematodes ; Nuclei ; Nuclei (cytology) ; Original ; OsBetvI ; OsCRRSP55 ; OsGSC ; Parasitic diseases ; Parasitism ; Pathogenesis ; Plant cells ; Proteins ; Protoplasts ; Reactive oxygen species ; Ribonucleic acid ; Rice ; RNA ; RNA-mediated interference ; Vectors ; Yeast ; β-Glucan</subject><ispartof>Molecular plant pathology, 2018-08, Vol.19 (8), p.1942-1955</ispartof><rights>2018 BSPP AND JOHN WILEY & SONS LTD</rights><rights>2018 BSPP AND JOHN WILEY & SONS LTD.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4431-8ed24d51d3b05ebd522e2acfebbbae2074a98aa50f74d3ee227253a345c7a27a3</citedby><cites>FETCH-LOGICAL-c4431-8ed24d51d3b05ebd522e2acfebbbae2074a98aa50f74d3ee227253a345c7a27a3</cites><orcidid>0000-0002-4175-7750</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6638000/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6638000/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1111%2Fmpp.12671$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29485753$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Jiansong</creatorcontrib><creatorcontrib>Hu, Lili</creatorcontrib><creatorcontrib>Sun, Longhua</creatorcontrib><creatorcontrib>Lin, Borong</creatorcontrib><creatorcontrib>Huang, Kun</creatorcontrib><creatorcontrib>Zhuo, Kan</creatorcontrib><creatorcontrib>Liao, Jinling</creatorcontrib><title>A novel Meloidogyne graminicola effector, MgMO237, interacts with multiple host defence‐related proteins to manipulate plant basal immunity and promote parasitism</title><title>Molecular plant pathology</title><addtitle>Mol Plant Pathol</addtitle><description>Summary
Plant‐parasitic nematodes can secrete effector proteins into the host tissue to facilitate their parasitism. In this study, we report a novel effector protein, MgMO237, from Meloidogyne graminicola, which is exclusively expressed within the dorsal oesophageal gland cell and markedly up‐regulated in parasitic third‐/fourth‐stage juveniles of M. graminicola. Transient expression of MgMO237 in protoplasts from rice roots showed that MgMO237 was localized in the cytoplasm and nucleus of the host cells. Rice plants overexpressing MgMO237 showed an increased susceptibility to M. graminicola. In contrast, rice plants expressing RNA interference vectors targeting MgMO237 showed an increased resistance to M. graminicola. In addition, yeast two‐hybrid and co‐immunoprecipitation assays showed that MgMO237 interacted specifically with three rice endogenous proteins, i.e. 1,3‐β‐glucan synthase component (OsGSC), cysteine‐rich repeat secretory protein 55 (OsCRRSP55) and pathogenesis‐related BetvI family protein (OsBetvI), which are all related to host defences. Moreover, MgMO237 can suppress host defence responses, including the expression of host defence‐related genes, cell wall callose deposition and the burst of reactive oxygen species. These results demonstrate that the effector MgMO237 probably promotes the parasitism of M. graminicola by interacting with multiple host defence‐related proteins and suppressing plant basal immunity in the later parasitic stages of nematodes.</description><subject>Cytoplasm</subject><subject>effector</subject><subject>Esophagus</subject><subject>Gene expression</subject><subject>Glucan</subject><subject>host defence suppression</subject><subject>Host plants</subject><subject>Immunity</subject><subject>Immunoprecipitation</subject><subject>interaction</subject><subject>Meloidogyne graminicola</subject><subject>Nematodes</subject><subject>Nuclei</subject><subject>Nuclei (cytology)</subject><subject>Original</subject><subject>OsBetvI</subject><subject>OsCRRSP55</subject><subject>OsGSC</subject><subject>Parasitic diseases</subject><subject>Parasitism</subject><subject>Pathogenesis</subject><subject>Plant cells</subject><subject>Proteins</subject><subject>Protoplasts</subject><subject>Reactive oxygen species</subject><subject>Ribonucleic acid</subject><subject>Rice</subject><subject>RNA</subject><subject>RNA-mediated interference</subject><subject>Vectors</subject><subject>Yeast</subject><subject>β-Glucan</subject><issn>1464-6722</issn><issn>1364-3703</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kUtuFDEQhlsIREJgwQWQJVaRMonbj3bPBimKeEkZJQtYW9Xd1TOO_Ghsd6LZcQQOwck4CZ5MiGBBbapkf_qqpL-qXtf0tC515qbptGaNqp9UhzVvxIIryp-WWZS5UYwdVC9SuqG0Vksmn1cHbClaqSQ_rH6eEx9u0ZIV2mCGsN56JOsIznjTBwsExxH7HOIJWa1XV4yrE2J8xgh9TuTO5A1xs81mskg2IWUy4Ii-x1_ff0S0kHEgUwwZjU8kB-LAm2nevZPJgs-kgwSWGOdmb_KWgL_nXdgBECGZbJJ7WT0bwSZ89dCPqq8f3n-5-LS4vPr4-eL8ctELwetFiwMTg6wH3lGJ3SAZQwb9iF3XATKqBCxbAElHJQaOyJhikgMXslfAFPCj6t3eO82dw6FHnyNYPUXjIG51AKP__fFmo9fhVjcNbymlRfD2QRDDtxlT1jdhjr7crMt6Jtoll7JQx3uqjyGliOPjhprqXaC6BKrvAy3sm79PeiT_JFiAsz1wZyxu_2_Sq-vrvfI3Ywiwyg</recordid><startdate>201808</startdate><enddate>201808</enddate><creator>Chen, Jiansong</creator><creator>Hu, Lili</creator><creator>Sun, Longhua</creator><creator>Lin, Borong</creator><creator>Huang, Kun</creator><creator>Zhuo, Kan</creator><creator>Liao, Jinling</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4175-7750</orcidid></search><sort><creationdate>201808</creationdate><title>A novel Meloidogyne graminicola effector, MgMO237, interacts with multiple host defence‐related proteins to manipulate plant basal immunity and promote parasitism</title><author>Chen, Jiansong ; Hu, Lili ; Sun, Longhua ; Lin, Borong ; Huang, Kun ; Zhuo, Kan ; Liao, Jinling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4431-8ed24d51d3b05ebd522e2acfebbbae2074a98aa50f74d3ee227253a345c7a27a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Cytoplasm</topic><topic>effector</topic><topic>Esophagus</topic><topic>Gene expression</topic><topic>Glucan</topic><topic>host defence suppression</topic><topic>Host plants</topic><topic>Immunity</topic><topic>Immunoprecipitation</topic><topic>interaction</topic><topic>Meloidogyne graminicola</topic><topic>Nematodes</topic><topic>Nuclei</topic><topic>Nuclei (cytology)</topic><topic>Original</topic><topic>OsBetvI</topic><topic>OsCRRSP55</topic><topic>OsGSC</topic><topic>Parasitic diseases</topic><topic>Parasitism</topic><topic>Pathogenesis</topic><topic>Plant cells</topic><topic>Proteins</topic><topic>Protoplasts</topic><topic>Reactive oxygen species</topic><topic>Ribonucleic acid</topic><topic>Rice</topic><topic>RNA</topic><topic>RNA-mediated interference</topic><topic>Vectors</topic><topic>Yeast</topic><topic>β-Glucan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Jiansong</creatorcontrib><creatorcontrib>Hu, Lili</creatorcontrib><creatorcontrib>Sun, Longhua</creatorcontrib><creatorcontrib>Lin, Borong</creatorcontrib><creatorcontrib>Huang, Kun</creatorcontrib><creatorcontrib>Zhuo, Kan</creatorcontrib><creatorcontrib>Liao, Jinling</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Chen, Jiansong</au><au>Hu, Lili</au><au>Sun, Longhua</au><au>Lin, Borong</au><au>Huang, Kun</au><au>Zhuo, Kan</au><au>Liao, Jinling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel Meloidogyne graminicola effector, MgMO237, interacts with multiple host defence‐related proteins to manipulate plant basal immunity and promote parasitism</atitle><jtitle>Molecular plant pathology</jtitle><addtitle>Mol Plant Pathol</addtitle><date>2018-08</date><risdate>2018</risdate><volume>19</volume><issue>8</issue><spage>1942</spage><epage>1955</epage><pages>1942-1955</pages><issn>1464-6722</issn><eissn>1364-3703</eissn><abstract>Summary
Plant‐parasitic nematodes can secrete effector proteins into the host tissue to facilitate their parasitism. In this study, we report a novel effector protein, MgMO237, from Meloidogyne graminicola, which is exclusively expressed within the dorsal oesophageal gland cell and markedly up‐regulated in parasitic third‐/fourth‐stage juveniles of M. graminicola. Transient expression of MgMO237 in protoplasts from rice roots showed that MgMO237 was localized in the cytoplasm and nucleus of the host cells. Rice plants overexpressing MgMO237 showed an increased susceptibility to M. graminicola. In contrast, rice plants expressing RNA interference vectors targeting MgMO237 showed an increased resistance to M. graminicola. In addition, yeast two‐hybrid and co‐immunoprecipitation assays showed that MgMO237 interacted specifically with three rice endogenous proteins, i.e. 1,3‐β‐glucan synthase component (OsGSC), cysteine‐rich repeat secretory protein 55 (OsCRRSP55) and pathogenesis‐related BetvI family protein (OsBetvI), which are all related to host defences. Moreover, MgMO237 can suppress host defence responses, including the expression of host defence‐related genes, cell wall callose deposition and the burst of reactive oxygen species. These results demonstrate that the effector MgMO237 probably promotes the parasitism of M. graminicola by interacting with multiple host defence‐related proteins and suppressing plant basal immunity in the later parasitic stages of nematodes.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>29485753</pmid><doi>10.1111/mpp.12671</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-4175-7750</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Cytoplasm effector Esophagus Gene expression Glucan host defence suppression Host plants Immunity Immunoprecipitation interaction Meloidogyne graminicola Nematodes Nuclei Nuclei (cytology) Original OsBetvI OsCRRSP55 OsGSC Parasitic diseases Parasitism Pathogenesis Plant cells Proteins Protoplasts Reactive oxygen species Ribonucleic acid Rice RNA RNA-mediated interference Vectors Yeast β-Glucan |
| title | A novel Meloidogyne graminicola effector, MgMO237, interacts with multiple host defence‐related proteins to manipulate plant basal immunity and promote parasitism |
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