The Gossypium hirsutum TIR‐NBS‐LRR gene GhDSC1 mediates resistance against Verticillium wilt
Summary Improving genetic resistance is a preferred method to manage Verticillium wilt of cotton and other hosts. Identifying host resistance is difficult because of the dearth of resistance genes against this pathogen. Previously, a novel candidate gene involved in Verticillium wilt resistance was...
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| Veröffentlicht in: | Molecular plant pathology 2019-06, Vol.20 (6), p.857-876 |
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| creator | Li, Ting‐Gang Wang, Bao‐Li Yin, Chun‐Mei Zhang, Dan‐Dan Wang, Dan Song, Jian Zhou, Lei Kong, Zhi‐Qiang Klosterman, Steven J. Li, Jun‐Jiao Adamu, Sabiu Liu, Ting‐Li Subbarao, Krishna V. Chen, Jie‐Yin Dai, Xiao‐Feng |
| description | Summary
Improving genetic resistance is a preferred method to manage Verticillium wilt of cotton and other hosts. Identifying host resistance is difficult because of the dearth of resistance genes against this pathogen. Previously, a novel candidate gene involved in Verticillium wilt resistance was identified by a genome‐wide association study using a panel of Gossypium hirsutum accessions. In this study, we cloned the candidate resistance gene from cotton that encodes a protein sharing homology with the TIR‐NBS‐LRR receptor‐like defence protein DSC1 in Arabidopsis thaliana (hereafter named GhDSC1). GhDSC1 expressed at higher levels in response to Verticillium wilt and jasmonic acid (JA) treatment in resistant cotton cultivars as compared to susceptible cultivars and its product was localized to nucleus. The transfer of GhDSC1 to Arabidopsis conferred Verticillium resistance in an A. thaliana dsc1 mutant. This resistance response was associated with reactive oxygen species (ROS) accumulation and increased expression of JA‐signalling‐related genes. Furthermore, the expression of GhDSC1 in response to Verticillium wilt and JA signalling in A. thaliana displayed expression patterns similar to GhCAMTA3 in cotton under identical conditions, suggesting a coordinated DSC1 and CAMTA3 response in A. thaliana to Verticillium wilt. Analyses of GhDSC1 sequence polymorphism revealed a single nucleotide polymorphism (SNP) difference between resistant and susceptible cotton accessions, within the P‐loop motif encoded by GhDSC1. This SNP difference causes ineffective activation of defence response in susceptible cultivars. These results demonstrated that GhDSC1 confers Verticillium resistance in the model plant system of A. thaliana, and therefore represents a suitable candidate for the genetic engineering of Verticillium wilt resistance in cotton. |
| doi_str_mv | 10.1111/mpp.12797 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6637886</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A732108425</galeid><sourcerecordid>A732108425</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5437-cd74ca4ed05b6948124ccab9f5e8d0a93edb3da1e16bbb228ba6776cc4dc8b863</originalsourceid><addsrcrecordid>eNp1ks1u1DAQxy0Eoh9w4AVQJC5w2K2_YscXpHaBUmmBartwNY4z2XWVOMFOivbGI_CMPAnebikUgS15RvZv_vZ4BqEnBE9JGkdt308JlUreQ_uECT5hErP7yefJF5LSPXQQ4yXGRCqaP0R7DKtcKk730eflGrLTLsZN78Y2W7sQxyE5y7PFj2_f359cpHW-WGQr8Ilbv7qYkayFypkBYhYgujgYbyEzK-N8HLJPEAZnXdNs1b66ZniEHtSmifD4xh6ij29eL2dvJ_MPp2ez4_nE5pzJia0kt4ZDhfNSKF4Qyq01papzKCpsFIOqZJUhQERZlpQWpRFSCmt5ZYuyEOwQvdzp9mOZHmjBD8E0ug-uNWGjO-P03RPv1nrVXWkhmCyuBZ7fCITuywhx0K2LFprGeOjGqCnDOZFCKJbQZ3-hl90YfEpP0y2mCqHUb2plGtDO1126125F9bFklOCC0zxR039QaVbQOtt5qF3avxPwYhdgQypbgPo2R4L1th10agd93Q6Jffrnp9ySv-qfgKMdkEoFm_8r6Xfn5zvJn4uUwLM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2230598699</pqid></control><display><type>article</type><title>The Gossypium hirsutum TIR‐NBS‐LRR gene GhDSC1 mediates resistance against Verticillium wilt</title><source>MEDLINE</source><source>Wiley Online Library Open Access</source><source>DOAJ Directory of Open Access Journals</source><source>Wiley Online Library Journals Frontfile Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Li, Ting‐Gang ; Wang, Bao‐Li ; Yin, Chun‐Mei ; Zhang, Dan‐Dan ; Wang, Dan ; Song, Jian ; Zhou, Lei ; Kong, Zhi‐Qiang ; Klosterman, Steven J. ; Li, Jun‐Jiao ; Adamu, Sabiu ; Liu, Ting‐Li ; Subbarao, Krishna V. ; Chen, Jie‐Yin ; Dai, Xiao‐Feng</creator><creatorcontrib>Li, Ting‐Gang ; Wang, Bao‐Li ; Yin, Chun‐Mei ; Zhang, Dan‐Dan ; Wang, Dan ; Song, Jian ; Zhou, Lei ; Kong, Zhi‐Qiang ; Klosterman, Steven J. ; Li, Jun‐Jiao ; Adamu, Sabiu ; Liu, Ting‐Li ; Subbarao, Krishna V. ; Chen, Jie‐Yin ; Dai, Xiao‐Feng</creatorcontrib><description>Summary
Improving genetic resistance is a preferred method to manage Verticillium wilt of cotton and other hosts. Identifying host resistance is difficult because of the dearth of resistance genes against this pathogen. Previously, a novel candidate gene involved in Verticillium wilt resistance was identified by a genome‐wide association study using a panel of Gossypium hirsutum accessions. In this study, we cloned the candidate resistance gene from cotton that encodes a protein sharing homology with the TIR‐NBS‐LRR receptor‐like defence protein DSC1 in Arabidopsis thaliana (hereafter named GhDSC1). GhDSC1 expressed at higher levels in response to Verticillium wilt and jasmonic acid (JA) treatment in resistant cotton cultivars as compared to susceptible cultivars and its product was localized to nucleus. The transfer of GhDSC1 to Arabidopsis conferred Verticillium resistance in an A. thaliana dsc1 mutant. This resistance response was associated with reactive oxygen species (ROS) accumulation and increased expression of JA‐signalling‐related genes. Furthermore, the expression of GhDSC1 in response to Verticillium wilt and JA signalling in A. thaliana displayed expression patterns similar to GhCAMTA3 in cotton under identical conditions, suggesting a coordinated DSC1 and CAMTA3 response in A. thaliana to Verticillium wilt. Analyses of GhDSC1 sequence polymorphism revealed a single nucleotide polymorphism (SNP) difference between resistant and susceptible cotton accessions, within the P‐loop motif encoded by GhDSC1. This SNP difference causes ineffective activation of defence response in susceptible cultivars. These results demonstrated that GhDSC1 confers Verticillium resistance in the model plant system of A. thaliana, and therefore represents a suitable candidate for the genetic engineering of Verticillium wilt resistance in cotton.</description><identifier>ISSN: 1464-6722</identifier><identifier>EISSN: 1364-3703</identifier><identifier>DOI: 10.1111/mpp.12797</identifier><identifier>PMID: 30957942</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Acid resistance ; Analysis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis - microbiology ; Arabidopsis thaliana ; calmodulin binding transcription activator (CAMTA) ; Cotton ; Cultivars ; Disease Resistance - physiology ; Drug resistance in microorganisms ; Gene expression ; Gene Expression Regulation, Plant - physiology ; Genes ; Genetic engineering ; Genetic research ; genetic resistance ; Genetically modified organisms ; Genome-wide association studies ; Genome-Wide Association Study ; Genomes ; Genomics ; Gossypium - genetics ; Gossypium - metabolism ; Gossypium - microbiology ; Gossypium hirsutum ; Homology ; hosts ; Jasmonic acid ; mutants ; NBS-LRR gene ; nonsynonymous mutation ; Original ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Plants, Genetically Modified - microbiology ; Polymorphism ; Proteins ; Reactive oxygen species ; resistance genes ; Signaling ; Single nucleotide polymorphisms ; Single-nucleotide polymorphism ; TIR‐NBS‐LRR gene ; Verticillium ; Verticillium - pathogenicity ; Verticillium wilt</subject><ispartof>Molecular plant pathology, 2019-06, Vol.20 (6), p.857-876</ispartof><rights>2019 The Authors. published by British Society for Plant Pathology and John Wiley & Sons Ltd</rights><rights>2019 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.</rights><rights>COPYRIGHT 2019 John Wiley & Sons, Inc.</rights><rights>2019 BSPP and John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5437-cd74ca4ed05b6948124ccab9f5e8d0a93edb3da1e16bbb228ba6776cc4dc8b863</citedby><cites>FETCH-LOGICAL-c5437-cd74ca4ed05b6948124ccab9f5e8d0a93edb3da1e16bbb228ba6776cc4dc8b863</cites><orcidid>0000-0002-2075-1835 ; 0000-0002-8040-099X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637886/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637886/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30957942$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Ting‐Gang</creatorcontrib><creatorcontrib>Wang, Bao‐Li</creatorcontrib><creatorcontrib>Yin, Chun‐Mei</creatorcontrib><creatorcontrib>Zhang, Dan‐Dan</creatorcontrib><creatorcontrib>Wang, Dan</creatorcontrib><creatorcontrib>Song, Jian</creatorcontrib><creatorcontrib>Zhou, Lei</creatorcontrib><creatorcontrib>Kong, Zhi‐Qiang</creatorcontrib><creatorcontrib>Klosterman, Steven J.</creatorcontrib><creatorcontrib>Li, Jun‐Jiao</creatorcontrib><creatorcontrib>Adamu, Sabiu</creatorcontrib><creatorcontrib>Liu, Ting‐Li</creatorcontrib><creatorcontrib>Subbarao, Krishna V.</creatorcontrib><creatorcontrib>Chen, Jie‐Yin</creatorcontrib><creatorcontrib>Dai, Xiao‐Feng</creatorcontrib><title>The Gossypium hirsutum TIR‐NBS‐LRR gene GhDSC1 mediates resistance against Verticillium wilt</title><title>Molecular plant pathology</title><addtitle>Mol Plant Pathol</addtitle><description>Summary
Improving genetic resistance is a preferred method to manage Verticillium wilt of cotton and other hosts. Identifying host resistance is difficult because of the dearth of resistance genes against this pathogen. Previously, a novel candidate gene involved in Verticillium wilt resistance was identified by a genome‐wide association study using a panel of Gossypium hirsutum accessions. In this study, we cloned the candidate resistance gene from cotton that encodes a protein sharing homology with the TIR‐NBS‐LRR receptor‐like defence protein DSC1 in Arabidopsis thaliana (hereafter named GhDSC1). GhDSC1 expressed at higher levels in response to Verticillium wilt and jasmonic acid (JA) treatment in resistant cotton cultivars as compared to susceptible cultivars and its product was localized to nucleus. The transfer of GhDSC1 to Arabidopsis conferred Verticillium resistance in an A. thaliana dsc1 mutant. This resistance response was associated with reactive oxygen species (ROS) accumulation and increased expression of JA‐signalling‐related genes. Furthermore, the expression of GhDSC1 in response to Verticillium wilt and JA signalling in A. thaliana displayed expression patterns similar to GhCAMTA3 in cotton under identical conditions, suggesting a coordinated DSC1 and CAMTA3 response in A. thaliana to Verticillium wilt. Analyses of GhDSC1 sequence polymorphism revealed a single nucleotide polymorphism (SNP) difference between resistant and susceptible cotton accessions, within the P‐loop motif encoded by GhDSC1. This SNP difference causes ineffective activation of defence response in susceptible cultivars. These results demonstrated that GhDSC1 confers Verticillium resistance in the model plant system of A. thaliana, and therefore represents a suitable candidate for the genetic engineering of Verticillium wilt resistance in cotton.</description><subject>Acid resistance</subject><subject>Analysis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis - microbiology</subject><subject>Arabidopsis thaliana</subject><subject>calmodulin binding transcription activator (CAMTA)</subject><subject>Cotton</subject><subject>Cultivars</subject><subject>Disease Resistance - physiology</subject><subject>Drug resistance in microorganisms</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant - physiology</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetic research</subject><subject>genetic resistance</subject><subject>Genetically modified organisms</subject><subject>Genome-wide association studies</subject><subject>Genome-Wide Association Study</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Gossypium - genetics</subject><subject>Gossypium - metabolism</subject><subject>Gossypium - microbiology</subject><subject>Gossypium hirsutum</subject><subject>Homology</subject><subject>hosts</subject><subject>Jasmonic acid</subject><subject>mutants</subject><subject>NBS-LRR gene</subject><subject>nonsynonymous mutation</subject><subject>Original</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Plants, Genetically Modified - microbiology</subject><subject>Polymorphism</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>resistance genes</subject><subject>Signaling</subject><subject>Single nucleotide polymorphisms</subject><subject>Single-nucleotide polymorphism</subject><subject>TIR‐NBS‐LRR gene</subject><subject>Verticillium</subject><subject>Verticillium - pathogenicity</subject><subject>Verticillium wilt</subject><issn>1464-6722</issn><issn>1364-3703</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>EIF</sourceid><recordid>eNp1ks1u1DAQxy0Eoh9w4AVQJC5w2K2_YscXpHaBUmmBartwNY4z2XWVOMFOivbGI_CMPAnebikUgS15RvZv_vZ4BqEnBE9JGkdt308JlUreQ_uECT5hErP7yefJF5LSPXQQ4yXGRCqaP0R7DKtcKk730eflGrLTLsZN78Y2W7sQxyE5y7PFj2_f359cpHW-WGQr8Ilbv7qYkayFypkBYhYgujgYbyEzK-N8HLJPEAZnXdNs1b66ZniEHtSmifD4xh6ij29eL2dvJ_MPp2ez4_nE5pzJia0kt4ZDhfNSKF4Qyq01papzKCpsFIOqZJUhQERZlpQWpRFSCmt5ZYuyEOwQvdzp9mOZHmjBD8E0ug-uNWGjO-P03RPv1nrVXWkhmCyuBZ7fCITuywhx0K2LFprGeOjGqCnDOZFCKJbQZ3-hl90YfEpP0y2mCqHUb2plGtDO1126125F9bFklOCC0zxR039QaVbQOtt5qF3avxPwYhdgQypbgPo2R4L1th10agd93Q6Jffrnp9ySv-qfgKMdkEoFm_8r6Xfn5zvJn4uUwLM</recordid><startdate>201906</startdate><enddate>201906</enddate><creator>Li, Ting‐Gang</creator><creator>Wang, Bao‐Li</creator><creator>Yin, Chun‐Mei</creator><creator>Zhang, Dan‐Dan</creator><creator>Wang, Dan</creator><creator>Song, Jian</creator><creator>Zhou, Lei</creator><creator>Kong, Zhi‐Qiang</creator><creator>Klosterman, Steven J.</creator><creator>Li, Jun‐Jiao</creator><creator>Adamu, Sabiu</creator><creator>Liu, Ting‐Li</creator><creator>Subbarao, Krishna V.</creator><creator>Chen, Jie‐Yin</creator><creator>Dai, Xiao‐Feng</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2075-1835</orcidid><orcidid>https://orcid.org/0000-0002-8040-099X</orcidid></search><sort><creationdate>201906</creationdate><title>The Gossypium hirsutum TIR‐NBS‐LRR gene GhDSC1 mediates resistance against Verticillium wilt</title><author>Li, Ting‐Gang ; Wang, Bao‐Li ; Yin, Chun‐Mei ; Zhang, Dan‐Dan ; Wang, Dan ; Song, Jian ; Zhou, Lei ; Kong, Zhi‐Qiang ; Klosterman, Steven J. ; Li, Jun‐Jiao ; Adamu, Sabiu ; Liu, Ting‐Li ; Subbarao, Krishna V. ; Chen, Jie‐Yin ; Dai, Xiao‐Feng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5437-cd74ca4ed05b6948124ccab9f5e8d0a93edb3da1e16bbb228ba6776cc4dc8b863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acid resistance</topic><topic>Analysis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis - microbiology</topic><topic>Arabidopsis thaliana</topic><topic>calmodulin binding transcription activator (CAMTA)</topic><topic>Cotton</topic><topic>Cultivars</topic><topic>Disease Resistance - physiology</topic><topic>Drug resistance in microorganisms</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant - physiology</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genetic research</topic><topic>genetic resistance</topic><topic>Genetically modified organisms</topic><topic>Genome-wide association studies</topic><topic>Genome-Wide Association Study</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Gossypium - genetics</topic><topic>Gossypium - metabolism</topic><topic>Gossypium - microbiology</topic><topic>Gossypium hirsutum</topic><topic>Homology</topic><topic>hosts</topic><topic>Jasmonic acid</topic><topic>mutants</topic><topic>NBS-LRR gene</topic><topic>nonsynonymous mutation</topic><topic>Original</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Plants, Genetically Modified - microbiology</topic><topic>Polymorphism</topic><topic>Proteins</topic><topic>Reactive oxygen species</topic><topic>resistance genes</topic><topic>Signaling</topic><topic>Single nucleotide polymorphisms</topic><topic>Single-nucleotide polymorphism</topic><topic>TIR‐NBS‐LRR gene</topic><topic>Verticillium</topic><topic>Verticillium - pathogenicity</topic><topic>Verticillium wilt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Ting‐Gang</creatorcontrib><creatorcontrib>Wang, Bao‐Li</creatorcontrib><creatorcontrib>Yin, Chun‐Mei</creatorcontrib><creatorcontrib>Zhang, Dan‐Dan</creatorcontrib><creatorcontrib>Wang, Dan</creatorcontrib><creatorcontrib>Song, Jian</creatorcontrib><creatorcontrib>Zhou, Lei</creatorcontrib><creatorcontrib>Kong, Zhi‐Qiang</creatorcontrib><creatorcontrib>Klosterman, Steven J.</creatorcontrib><creatorcontrib>Li, Jun‐Jiao</creatorcontrib><creatorcontrib>Adamu, Sabiu</creatorcontrib><creatorcontrib>Liu, Ting‐Li</creatorcontrib><creatorcontrib>Subbarao, Krishna V.</creatorcontrib><creatorcontrib>Chen, Jie‐Yin</creatorcontrib><creatorcontrib>Dai, Xiao‐Feng</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Ting‐Gang</au><au>Wang, Bao‐Li</au><au>Yin, Chun‐Mei</au><au>Zhang, Dan‐Dan</au><au>Wang, Dan</au><au>Song, Jian</au><au>Zhou, Lei</au><au>Kong, Zhi‐Qiang</au><au>Klosterman, Steven J.</au><au>Li, Jun‐Jiao</au><au>Adamu, Sabiu</au><au>Liu, Ting‐Li</au><au>Subbarao, Krishna V.</au><au>Chen, Jie‐Yin</au><au>Dai, Xiao‐Feng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Gossypium hirsutum TIR‐NBS‐LRR gene GhDSC1 mediates resistance against Verticillium wilt</atitle><jtitle>Molecular plant pathology</jtitle><addtitle>Mol Plant Pathol</addtitle><date>2019-06</date><risdate>2019</risdate><volume>20</volume><issue>6</issue><spage>857</spage><epage>876</epage><pages>857-876</pages><issn>1464-6722</issn><eissn>1364-3703</eissn><abstract>Summary
Improving genetic resistance is a preferred method to manage Verticillium wilt of cotton and other hosts. Identifying host resistance is difficult because of the dearth of resistance genes against this pathogen. Previously, a novel candidate gene involved in Verticillium wilt resistance was identified by a genome‐wide association study using a panel of Gossypium hirsutum accessions. In this study, we cloned the candidate resistance gene from cotton that encodes a protein sharing homology with the TIR‐NBS‐LRR receptor‐like defence protein DSC1 in Arabidopsis thaliana (hereafter named GhDSC1). GhDSC1 expressed at higher levels in response to Verticillium wilt and jasmonic acid (JA) treatment in resistant cotton cultivars as compared to susceptible cultivars and its product was localized to nucleus. The transfer of GhDSC1 to Arabidopsis conferred Verticillium resistance in an A. thaliana dsc1 mutant. This resistance response was associated with reactive oxygen species (ROS) accumulation and increased expression of JA‐signalling‐related genes. Furthermore, the expression of GhDSC1 in response to Verticillium wilt and JA signalling in A. thaliana displayed expression patterns similar to GhCAMTA3 in cotton under identical conditions, suggesting a coordinated DSC1 and CAMTA3 response in A. thaliana to Verticillium wilt. Analyses of GhDSC1 sequence polymorphism revealed a single nucleotide polymorphism (SNP) difference between resistant and susceptible cotton accessions, within the P‐loop motif encoded by GhDSC1. This SNP difference causes ineffective activation of defence response in susceptible cultivars. These results demonstrated that GhDSC1 confers Verticillium resistance in the model plant system of A. thaliana, and therefore represents a suitable candidate for the genetic engineering of Verticillium wilt resistance in cotton.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>30957942</pmid><doi>10.1111/mpp.12797</doi><tpages>20</tpages><orcidid>https://orcid.org/0000-0002-2075-1835</orcidid><orcidid>https://orcid.org/0000-0002-8040-099X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1464-6722 |
| ispartof | Molecular plant pathology, 2019-06, Vol.20 (6), p.857-876 |
| issn | 1464-6722 1364-3703 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6637886 |
| source | MEDLINE; Wiley Online Library Open Access; DOAJ Directory of Open Access Journals; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Acid resistance Analysis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis - microbiology Arabidopsis thaliana calmodulin binding transcription activator (CAMTA) Cotton Cultivars Disease Resistance - physiology Drug resistance in microorganisms Gene expression Gene Expression Regulation, Plant - physiology Genes Genetic engineering Genetic research genetic resistance Genetically modified organisms Genome-wide association studies Genome-Wide Association Study Genomes Genomics Gossypium - genetics Gossypium - metabolism Gossypium - microbiology Gossypium hirsutum Homology hosts Jasmonic acid mutants NBS-LRR gene nonsynonymous mutation Original Plant Diseases - genetics Plant Diseases - microbiology Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Plants, Genetically Modified - microbiology Polymorphism Proteins Reactive oxygen species resistance genes Signaling Single nucleotide polymorphisms Single-nucleotide polymorphism TIR‐NBS‐LRR gene Verticillium Verticillium - pathogenicity Verticillium wilt |
| title | The Gossypium hirsutum TIR‐NBS‐LRR gene GhDSC1 mediates resistance against Verticillium wilt |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T12%3A33%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Gossypium%20hirsutum%20TIR%E2%80%90NBS%E2%80%90LRR%20gene%20GhDSC1%20mediates%20resistance%20against%20Verticillium%20wilt&rft.jtitle=Molecular%20plant%20pathology&rft.au=Li,%20Ting%E2%80%90Gang&rft.date=2019-06&rft.volume=20&rft.issue=6&rft.spage=857&rft.epage=876&rft.pages=857-876&rft.issn=1464-6722&rft.eissn=1364-3703&rft_id=info:doi/10.1111/mpp.12797&rft_dat=%3Cgale_pubme%3EA732108425%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2230598699&rft_id=info:pmid/30957942&rft_galeid=A732108425&rfr_iscdi=true |