Network Pharmacology-Based Prediction and Verification of the Targets and Mechanism for Panax Notoginseng Saponins against Coronary Heart Disease
Coronary heart disease (CHD) is the worldwide leading cause for cardiovascular death. Panax notoginseng saponin (PNS), which is the main bioactive compound of panax notoginseng, has been generally accepted to exert a remarkable effect on CHD for a long time. However, to reveal the underlying treatme...
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| description | Coronary heart disease (CHD) is the worldwide leading cause for cardiovascular death. Panax notoginseng saponin (PNS), which is the main bioactive compound of panax notoginseng, has been generally accepted to exert a remarkable effect on CHD for a long time. However, to reveal the underlying treatment target and corresponding mechanism of PNS against CHD is still a substantial challenge. In this work, the targets and mechanism of PNS against CHD were successfully achieved by pharmacology-based prediction and experimental verification. 36 common targets were screened out through integrating the gene expression profile of CHD and the chemical-protein data of PNS. Then, two key nodes were further selected for verification by experiment after analyzing GO function, KEGG pathway, coexpression, and topology analysis. Results showed that PNS has protected the human umbilical vein endothelial cells from H2O2-induced oxidative stress by inhibiting early cell apoptosis via upregulating VEGFA mRNA expression. Therefore, our research has successfully pointed out one treatment target and apoptotic inhibition caused by PNS with method of integrating bioinformatics prediction and experimental verification, which has partially explained the pharmacological mechanism of PNS against CHD. |
| doi_str_mv | 10.1155/2019/6503752 |
| format | Article |
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Panax notoginseng saponin (PNS), which is the main bioactive compound of panax notoginseng, has been generally accepted to exert a remarkable effect on CHD for a long time. However, to reveal the underlying treatment target and corresponding mechanism of PNS against CHD is still a substantial challenge. In this work, the targets and mechanism of PNS against CHD were successfully achieved by pharmacology-based prediction and experimental verification. 36 common targets were screened out through integrating the gene expression profile of CHD and the chemical-protein data of PNS. Then, two key nodes were further selected for verification by experiment after analyzing GO function, KEGG pathway, coexpression, and topology analysis. Results showed that PNS has protected the human umbilical vein endothelial cells from H2O2-induced oxidative stress by inhibiting early cell apoptosis via upregulating VEGFA mRNA expression. Therefore, our research has successfully pointed out one treatment target and apoptotic inhibition caused by PNS with method of integrating bioinformatics prediction and experimental verification, which has partially explained the pharmacological mechanism of PNS against CHD.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2019/6503752</identifier><identifier>PMID: 31354855</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Angina pectoris ; Apoptosis ; Arteriosclerosis ; Bioactive compounds ; Bioinformatics ; Brain research ; Cardiovascular disease ; Cardiovascular diseases ; Chinese medicine ; Coronary artery disease ; Drugs ; Endothelial cells ; Gene expression ; Heart attacks ; Heart diseases ; Hydrogen peroxide ; Kinases ; Lipids ; Metabolism ; Metabolites ; Oxidative stress ; Panax notoginseng ; Pharmacology ; Proteins ; Saponins ; Thrombosis ; Topology ; Umbilical vein</subject><ispartof>Evidence-based complementary and alternative medicine, 2019-01, Vol.2019 (2019), p.1-13</ispartof><rights>Copyright © 2019 Yan Dong et al.</rights><rights>COPYRIGHT 2019 John Wiley & Sons, Inc.</rights><rights>Copyright © 2019 Yan Dong et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2019 Yan Dong et al. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-cd1463df21d76ad783ad3a0aa091bbc8cfe44a8f6637b112afc518b646a16e8b3</citedby><cites>FETCH-LOGICAL-c499t-cd1463df21d76ad783ad3a0aa091bbc8cfe44a8f6637b112afc518b646a16e8b3</cites><orcidid>0000-0001-7870-0646</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6636530/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6636530/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31354855$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Cho, Jae Youl</contributor><contributor>Jae Youl Cho</contributor><creatorcontrib>Zhang, Yun</creatorcontrib><creatorcontrib>Liu, Yong-mei</creatorcontrib><creatorcontrib>Chen, Heng-Wen</creatorcontrib><creatorcontrib>Duan, Lian</creatorcontrib><creatorcontrib>Dong, Yan</creatorcontrib><creatorcontrib>Wang, Jie</creatorcontrib><title>Network Pharmacology-Based Prediction and Verification of the Targets and Mechanism for Panax Notoginseng Saponins against Coronary Heart Disease</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Coronary heart disease (CHD) is the worldwide leading cause for cardiovascular death. Panax notoginseng saponin (PNS), which is the main bioactive compound of panax notoginseng, has been generally accepted to exert a remarkable effect on CHD for a long time. However, to reveal the underlying treatment target and corresponding mechanism of PNS against CHD is still a substantial challenge. In this work, the targets and mechanism of PNS against CHD were successfully achieved by pharmacology-based prediction and experimental verification. 36 common targets were screened out through integrating the gene expression profile of CHD and the chemical-protein data of PNS. Then, two key nodes were further selected for verification by experiment after analyzing GO function, KEGG pathway, coexpression, and topology analysis. Results showed that PNS has protected the human umbilical vein endothelial cells from H2O2-induced oxidative stress by inhibiting early cell apoptosis via upregulating VEGFA mRNA expression. Therefore, our research has successfully pointed out one treatment target and apoptotic inhibition caused by PNS with method of integrating bioinformatics prediction and experimental verification, which has partially explained the pharmacological mechanism of PNS against CHD.</description><subject>Angina pectoris</subject><subject>Apoptosis</subject><subject>Arteriosclerosis</subject><subject>Bioactive compounds</subject><subject>Bioinformatics</subject><subject>Brain research</subject><subject>Cardiovascular disease</subject><subject>Cardiovascular diseases</subject><subject>Chinese medicine</subject><subject>Coronary artery disease</subject><subject>Drugs</subject><subject>Endothelial cells</subject><subject>Gene expression</subject><subject>Heart attacks</subject><subject>Heart diseases</subject><subject>Hydrogen peroxide</subject><subject>Kinases</subject><subject>Lipids</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Oxidative stress</subject><subject>Panax notoginseng</subject><subject>Pharmacology</subject><subject>Proteins</subject><subject>Saponins</subject><subject>Thrombosis</subject><subject>Topology</subject><subject>Umbilical 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Pharmacology-Based Prediction and Verification of the Targets and Mechanism for Panax Notoginseng Saponins against Coronary Heart Disease</title><author>Zhang, Yun ; Liu, Yong-mei ; Chen, Heng-Wen ; Duan, Lian ; Dong, Yan ; Wang, Jie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-cd1463df21d76ad783ad3a0aa091bbc8cfe44a8f6637b112afc518b646a16e8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Angina pectoris</topic><topic>Apoptosis</topic><topic>Arteriosclerosis</topic><topic>Bioactive compounds</topic><topic>Bioinformatics</topic><topic>Brain research</topic><topic>Cardiovascular disease</topic><topic>Cardiovascular diseases</topic><topic>Chinese medicine</topic><topic>Coronary artery disease</topic><topic>Drugs</topic><topic>Endothelial cells</topic><topic>Gene expression</topic><topic>Heart attacks</topic><topic>Heart diseases</topic><topic>Hydrogen peroxide</topic><topic>Kinases</topic><topic>Lipids</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Oxidative stress</topic><topic>Panax notoginseng</topic><topic>Pharmacology</topic><topic>Proteins</topic><topic>Saponins</topic><topic>Thrombosis</topic><topic>Topology</topic><topic>Umbilical vein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yun</creatorcontrib><creatorcontrib>Liu, Yong-mei</creatorcontrib><creatorcontrib>Chen, Heng-Wen</creatorcontrib><creatorcontrib>Duan, Lian</creatorcontrib><creatorcontrib>Dong, Yan</creatorcontrib><creatorcontrib>Wang, Jie</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing 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Complement Alternat Med</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>2019</volume><issue>2019</issue><spage>1</spage><epage>13</epage><pages>1-13</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Coronary heart disease (CHD) is the worldwide leading cause for cardiovascular death. Panax notoginseng saponin (PNS), which is the main bioactive compound of panax notoginseng, has been generally accepted to exert a remarkable effect on CHD for a long time. However, to reveal the underlying treatment target and corresponding mechanism of PNS against CHD is still a substantial challenge. In this work, the targets and mechanism of PNS against CHD were successfully achieved by pharmacology-based prediction and experimental verification. 36 common targets were screened out through integrating the gene expression profile of CHD and the chemical-protein data of PNS. Then, two key nodes were further selected for verification by experiment after analyzing GO function, KEGG pathway, coexpression, and topology analysis. Results showed that PNS has protected the human umbilical vein endothelial cells from H2O2-induced oxidative stress by inhibiting early cell apoptosis via upregulating VEGFA mRNA expression. Therefore, our research has successfully pointed out one treatment target and apoptotic inhibition caused by PNS with method of integrating bioinformatics prediction and experimental verification, which has partially explained the pharmacological mechanism of PNS against CHD.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>31354855</pmid><doi>10.1155/2019/6503752</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-7870-0646</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Angina pectoris Apoptosis Arteriosclerosis Bioactive compounds Bioinformatics Brain research Cardiovascular disease Cardiovascular diseases Chinese medicine Coronary artery disease Drugs Endothelial cells Gene expression Heart attacks Heart diseases Hydrogen peroxide Kinases Lipids Metabolism Metabolites Oxidative stress Panax notoginseng Pharmacology Proteins Saponins Thrombosis Topology Umbilical vein |
| title | Network Pharmacology-Based Prediction and Verification of the Targets and Mechanism for Panax Notoginseng Saponins against Coronary Heart Disease |
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