Post-Mortem Immunohistochemical Evidence of β2-Adrenergic Receptor Expression in the Adrenal Gland
The evidence from post-mortem biochemical studies conducted on cortisol and catecholamines suggest that analysis of the adrenal gland could provide useful information about its role in human pathophysiology and the stress response. Authors designed an immunohistochemical study on the expression of t...
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| Veröffentlicht in: | International journal of molecular sciences 2019-06, Vol.20 (12), p.3065 |
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| creator | Ventura Spagnolo, Elvira Mondello, Cristina Cardia, Luigi Minutoli, Letteria Puzzolo, Domenico Asmundo, Alessio Macaione, Vincenzo Alibrandi, Angela Malta, Consuelo Baldino, Gennaro Micali, Antonio |
| description | The evidence from post-mortem biochemical studies conducted on cortisol and catecholamines suggest that analysis of the adrenal gland could provide useful information about its role in human pathophysiology and the stress response. Authors designed an immunohistochemical study on the expression of the adrenal β2-adrenergic receptor (β2-AR), a receptor with high-affinity for catecholamines, with the aim to show which zones it is expressed in and how its expression differs in relation to the cause of death. The immunohistochemical study was performed on adrenal glands obtained from 48 forensic autopsies of subjects that died as a result of different pathogenic mechanisms using a mouse monoclonal β2-AR antibody. The results show that immunoreactivity for β2-AR was observed in all adrenal zones. Furthermore, immunoreactivity for β2-AR has shown variation in the localization and intensity of different patterns in relation to the original cause of death. To the best of our knowledge, this is the first study that demonstrates β2-AR expression in the human cortex and provides suggestions on the possible involvement of β2-AR in human cortex hormonal stimulation. In conclusion, the authors provide a possible explanation for the observed differences in expression in relation to the cause of death. |
| doi_str_mv | 10.3390/ijms20123065 |
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Authors designed an immunohistochemical study on the expression of the adrenal β2-adrenergic receptor (β2-AR), a receptor with high-affinity for catecholamines, with the aim to show which zones it is expressed in and how its expression differs in relation to the cause of death. The immunohistochemical study was performed on adrenal glands obtained from 48 forensic autopsies of subjects that died as a result of different pathogenic mechanisms using a mouse monoclonal β2-AR antibody. The results show that immunoreactivity for β2-AR was observed in all adrenal zones. Furthermore, immunoreactivity for β2-AR has shown variation in the localization and intensity of different patterns in relation to the original cause of death. To the best of our knowledge, this is the first study that demonstrates β2-AR expression in the human cortex and provides suggestions on the possible involvement of β2-AR in human cortex hormonal stimulation. In conclusion, the authors provide a possible explanation for the observed differences in expression in relation to the cause of death.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms20123065</identifier><identifier>PMID: 31234562</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adrenal glands ; Adrenergic receptors ; Adrenocorticotropic hormone ; Blood vessels ; Catecholamines ; Corticosteroids ; Death ; Fatalities ; Forensic pathology ; Forensic science ; Glucocorticoids ; Localization ; Morphology ; Nervous system ; Organs ; Proteins ; Receptors (physiology) ; Sepsis ; Statistical analysis ; Stimulation ; Stress response ; Studies</subject><ispartof>International journal of molecular sciences, 2019-06, Vol.20 (12), p.3065</ispartof><rights>2019. 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Authors designed an immunohistochemical study on the expression of the adrenal β2-adrenergic receptor (β2-AR), a receptor with high-affinity for catecholamines, with the aim to show which zones it is expressed in and how its expression differs in relation to the cause of death. The immunohistochemical study was performed on adrenal glands obtained from 48 forensic autopsies of subjects that died as a result of different pathogenic mechanisms using a mouse monoclonal β2-AR antibody. The results show that immunoreactivity for β2-AR was observed in all adrenal zones. Furthermore, immunoreactivity for β2-AR has shown variation in the localization and intensity of different patterns in relation to the original cause of death. To the best of our knowledge, this is the first study that demonstrates β2-AR expression in the human cortex and provides suggestions on the possible involvement of β2-AR in human cortex hormonal stimulation. 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| subjects | Adrenal glands Adrenergic receptors Adrenocorticotropic hormone Blood vessels Catecholamines Corticosteroids Death Fatalities Forensic pathology Forensic science Glucocorticoids Localization Morphology Nervous system Organs Proteins Receptors (physiology) Sepsis Statistical analysis Stimulation Stress response Studies |
| title | Post-Mortem Immunohistochemical Evidence of β2-Adrenergic Receptor Expression in the Adrenal Gland |
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